LOCUS       pLenti_pRRL-SV40        9636 bp DNA     circular SYN 27-MAR-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 9636)
  AUTHORS   Ortiz
  TITLE     Direct Submission
  JOURNAL   Exported Jul 12, 2024 from SnapGene 7.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9636
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     enhancer        21..324
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        325..528
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     479..499
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     525..549
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     582..625
                     /label=FW-mCherry-IF-LC3B
     regulatory      623..632
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      624..633
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             629..1336
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(774..792)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1042..1061
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    1364..1388
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1397..1406
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1403..1405,1406..1408,1409..2119)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1403..1405,1406..1408,1409..2119)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1403..1405,1406..1408,1409..2116)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     primer_bind     complement(1448..1469)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1709..1728)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2056..2077
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2147..2521
                     /codon_start=1
                     /gene="MAP1LC3B"
                     /product="human LC3 protein"
                     /label=LC3B
                     /note="Atg8 family member found on autophagosomes"
                     /translation="MPSEKTFKQRRTFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLP
                     VLDKTKFLVPDHVNMSELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESEKD
                     EDGFLYMVYASQETFGMKLSV"
     primer_bind     complement(2496..2543)
                     /label=RV-mCherry-LC3B-IF
     primer_bind     complement(2504..2524)
                     /label=RV-mCherry-LC3B
     misc_feature    2548..3136
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2601..2621)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3019..3030)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3227..3460
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3532..3653
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3569..3588)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3623..3642
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3693..3828
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3848..3867)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3849..3867)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3877..3894)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3877..3893)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3886..3908)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3985..3998
                     /label=FW-mCherry-IF-LC3B
     rep_origin      4035..4490
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4122..4141)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4332..4353
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4516..4620
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4621..4689,4690..5481)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4839..4858)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5652..6240
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6141..6160
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6394..6411
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6528..6549
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6564..6569,6570..6587,6588..6594)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6602..6618
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6607..6629
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6626..6642
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6626..6642
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6661..6681
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6663..6681
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6709..6935
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6936..7116
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7163..7288
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7781..8014
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8199..8243
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8510..8626
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     complement(8638..8658)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8643..8972
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8823..8958
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8982..9581
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8982..9001)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9478..9498
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 cgataagctt gggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
       61 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      121 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      181 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      241 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      301 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
      361 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
      421 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
      481 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
      541 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgactc
      601 tagaccgcta gcgctaccgg tcgccaccat ggtgagcaag ggcgaggagg ataacatggc
      661 catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga
      721 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
      781 gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat
      841 gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc
      901 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac
      961 cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg
     1021 caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
     1081 ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa
     1141 gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc
     1201 cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga
     1261 ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat
     1321 ggacgagctg tacaagtccg gactcagatc cgctggccca ggaacaagtt tgtacaaaaa
     1381 agcaggcttt ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt
     1441 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     1501 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     1561 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     1621 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     1681 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     1741 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     1801 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     1861 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     1921 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     1981 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
     2041 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     2101 catggacgag ctgtacaagt ccggactcag atccggtacc gaattcatgc cgtcggagaa
     2161 gaccttcaag cagcgccgca ccttcgaaca aagagtagaa gatgtccgac ttattcgaga
     2221 gcagcatcca accaaaatcc cggtgataat agaacgatac aagggtgaga agcagcttcc
     2281 tgttctggat aaaacaaagt tccttgtacc tgaccatgtc aacatgagtg agctcatcaa
     2341 gataattaga aggcgcttac agctcaatgc taatcaggcc ttcttcctgt tggtgaacgg
     2401 acacagcatg gtcagcgtct ccacaccaat ctcagaggtg tatgagagtg agaaagatga
     2461 agatggattc ctgtacatgg tctatgcctc ccaggagacg ttcgggatga aattgtcagt
     2521 gtaactcgag caccagaatt cgtcgacaat caacctctgg attacaaaat ttgtgaaaga
     2581 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     2641 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     2701 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     2761 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     2821 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     2881 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     2941 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     3001 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     3061 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     3121 tgggccgcct ccccgcctgg aattaattcg agctcggtac ctttaagacc aatgacttac
     3181 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     3241 cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
     3301 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     3361 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     3421 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt
     3481 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt
     3541 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     3601 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     3661 tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     3721 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     3781 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc
     3841 caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg
     3901 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     3961 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     4021 gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     4081 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     4141 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     4201 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     4261 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     4321 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     4381 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     4441 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact
     4501 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     4561 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     4621 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     4681 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     4741 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     4801 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     4861 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     4921 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     4981 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     5041 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     5101 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     5161 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     5221 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     5281 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     5341 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     5401 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     5461 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     5521 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     5581 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     5641 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     5701 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     5761 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     5821 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     5881 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     5941 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     6001 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     6061 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     6121 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     6181 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     6241 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     6301 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     6361 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     6421 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     6481 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     6541 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     6601 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc
     6661 gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg
     6721 caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag
     6781 agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag
     6841 gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag
     6901 atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct
     6961 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     7021 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     7081 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     7141 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     7201 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     7261 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     7321 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     7381 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     7441 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     7501 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     7561 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     7621 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     7681 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     7741 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     7801 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     7861 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     7921 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     7981 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     8041 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     8101 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     8161 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     8221 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     8281 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     8341 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     8401 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
     8461 gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact tttaaaagaa
     8521 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
     8581 tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg accctgtgga
     8641 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     8701 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     8761 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     8821 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     8881 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     8941 gaggcttttt tggaggccta ggcttttgca aaaagcttac catgaccgag tacaagccca
     9001 cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt
     9061 tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca
     9121 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
     9181 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
     9241 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
     9301 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
     9361 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
     9421 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
     9481 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
     9541 gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc cacgacccgc
     9601 agcgcccgac cgaaaggagc gcacgacccc atgcat
//
