LOCUS       Exported                7099 bp ds-DNA     circular SYN 27-JUN-2024
DEFINITION  pCAGGSsH-C.
ACCESSION   pCAGGSsH-C
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7099)
  AUTHORS   Self
  JOURNAL   Unpublished.
REFERENCE   2  (bases 1 to 7099)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jul 8, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     SECID/File created by Clone Manager, Scientific
FEATURES             Location/Qualifiers
     source          1..7099
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..1341
                     /gene="pAG"
                     /product="AG-promotor"
                     /label=AG-promotor
     misc_feature    370..1293
                     /gene="Intron pAG"
                     /product="Intron AG-promotor"
                     /label=Intron AG-promotor
     primer_bind     1247..1264
                     /label=SMP2546
     primer_bind     1254..1273
                     /label=SMP2581
     primer_bind     1254..1270
                     /label=SMP2817
     primer_bind     1295..1312
                     /label=SMP2571
     primer_bind     1334..1348
                     /label=SMP2574
     misc_signal     1353..1360
                     /gene="Kozack"
                     /label=Kozack
     CDS             1361..2068
                     /codon_start=1
                     /label=mCherry
                     /note="mCherry"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     1361..1380
                     /label=SMP2235
     primer_bind     1361..1380
                     /label=SMP3364
     primer_bind     1364..1381
                     /label=SMP2824
     primer_bind     complement(1364..1381)
                     /label=SMP3729
     primer_bind     complement(1399..1416)
                     /label=SMP289
     primer_bind     complement(1402..1421)
                     /label=SMP366
     primer_bind     complement(1492..1511)
                     /label=SMP496
     primer_bind     complement(1506..1524)
                     /label=SMP3613
     primer_bind     1685..1704
                     /label=SMP1664
     primer_bind     1720..1739
                     /label=SMP1695
     primer_bind     complement(1771..1794)
                     /label=SMP2765
     primer_bind     1774..1793
                     /label=SMP2283
     primer_bind     1774..1793
                     /label=SMP3614
     primer_bind     1790..1807
                     /label=SMP1583
     primer_bind     1821..1838
                     /label=SMP1581
     primer_bind     complement(1889..1908)
                     /label=SMP1822
     primer_bind     1897..1915
                     /label=SMP527
     primer_bind     1909..1928
                     /label=SMP1821
     primer_bind     1999..2018
                     /label=SMP365
     primer_bind     complement(2048..2068)
                     /label=SMP2429
     primer_bind     2048..2068
                     /label=SMP3728
     primer_bind     complement(2048..2067)
                     /label=SMP2596
     CDS             2075..3622
                     /codon_start=1
                     /label=ATG13
                     /note="ATG13"
                     /translation="ETDLNSQDRKDLDKFIKFFALKTVQVIVQARLGEKICTRSSSSPT
                     GSDWFNLAIKDIPEVTHEAKKALAGQLPAVGRSMCVEISLKTSEGDSMELEIWCLEMNE
                     KCDKEIKVSYTVYNRLSLLLKSLLAITRVTPAYRLSRKQGHEYVILYRIYFGEVQLSGL
                     GEGFQTVRVGTVGTPVGTITLSCAYRINLAFMSTRQFERTPPIMGIIIDHFVDRPYPSS
                     SPMHPCNYRTAGEDTGVIYPSVEDSQEVCTTSFSTSPPSQLSSSRLSYQPAALGVGSAD
                     LAYPVVFAAGLNATHPHQLMVPGKEGGVPLAPNQPVHGTQADQERLATCTPSDRTHCAA
                     TPSSSEDTETVSNSSEGRASPHDVLETIFVRKVGAFVNKPINQVTLTSLDIPFAMFAPK
                     NLELEDTDPMVNPPDSPETESPLQGSLHSDGSSGGSSGNTHDDFVMIDFKPAFSKDDIL
                     PMDLGTFYREFQNPPQLSSLSIDIGAQSMAEDLDSLPEKLAVHEKNVREFDAFVETLQ"
     CDS             2598..2609
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     2723..2740
                     /label=SMP2547
     primer_bind     complement(2860..2876)
                     /label=SMP2554
     misc_feature    3626..3649
                     /label=ccdB_F
     primer_bind     3626..3649
                     /label=SMP1609
     primer_bind     complement(3626..3643)
                     /label=SMP1807
     primer_bind     complement(3688..3703)
                     /label=SMP2553
     misc_feature    3734..3825
                     /gene="3'UTR"
                     /product="3' UTR of rabbit B-globin, incl. polyA"
                     /label=3' UTR of rabbit B-globin, incl. polyA
     primer_bind     complement(3784..3799)
                     /label=SMP2818
     misc_feature    3826..4182
                     /gene="3'FR"
                     /product="3' flanking region of rabbit B-globin"
                     /label=3' flanking region of rabbit B-globin
     primer_bind     complement(4213..4233)
                     /label=M13-rev
     primer_bind     complement(4217..4233)
                     /label=M13r
     primer_bind     complement(4219..4238)
                     /label=SMP993
     primer_bind     complement(4230..4250)
                     /label=SMP547
     primer_bind     complement(4231..4250)
                     /label=LacOp-for
     misc_binding    complement(4239..4261)
                     /label=LacO
     misc_feature    complement(4260..4342)
                     /gene="pLac"
                     /label=pLac
     primer_bind     complement(4265..4284)
                     /label=pUCM13-rev-157
     primer_bind     complement(4345..4362)
                     /label=SMP2212
     primer_bind     4419..4438
                     /label=SV40-for
     primer_bind     4440..4456
                     /label=SV40-for
     misc_feature    4484..4559
                     /gene="SV40 ori"
                     /label=SV40 ori
     misc_feature    4590..4664
                     /gene="SV40 polyA late"
                     /product="Terminator SV40 Poly-adenylation signal"
                     /label=Terminator SV40 Poly-adenylation signal
     primer_bind     complement(4590..4607)
                     /label=EGFP-C-Rev
     primer_bind     4627..4648
                     /label=pCI-rev
     primer_bind     complement(4631..4650)
                     /label=SMP3314
     primer_bind     4681..4700
                     /label=EBV-rev
     misc_feature    complement(4779..5180)
                     /gene="pMB1 ori"
                     /product="pMB1 origin of replication of Escherichia coli"
                     /label=pMB1 origin of replication of Escherichia coli
     rep_origin      complement(4944..5626)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(5721..6581)
                     /gene="Ampicillin"
                     /product="Ampicillin"
                     /label=Ampicillin
     primer_bind     complement(5771..5790)
                     /label=AmpStop
     misc_feature    complement(5865)
                     /label=silent C to eliminate BsaI
     primer_bind     6517..6536
                     /label=AmpStart
     misc_feature    6623..6651
                     /label=AmpR promoter
     primer_bind     complement(6667..6687)
                     /label=SMP646
     primer_bind     6667..6687
                     /label=SMP654
     misc_feature    6717..7094
                     /gene="hCMV-IE enhancer"
                     /product="hCMV-IE enhancer"
                     /label=hCMV-IE enhancer
     primer_bind     complement(6938..6959)
                     /label=SMP1803
ORIGIN
        1 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt
       61 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg gggggggggg
      121 ccccccccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gaaaggtgcg
      181 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg
      241 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgttgcgcg ctgccttccc
      301 cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta
      361 ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta gcgcttggtt
      421 taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc
      481 cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc
      541 gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt
      601 gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg
      661 ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt
      721 gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc ctccccgagt tgctgagcac
      781 ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc
      841 ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc cggggagggc
      901 tcgggggagg ggcgcggcgg cccccggagc gccggcggct gtcgaggcgc ggcgagccgc
      961 agccattgcc ttttatggta atcgtgcgag agggcgcagg gacttccttt gtcccaaatc
     1021 tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag
     1081 cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc
     1141 cgtccccttc tccctctcca gcctcggggc tgtccgcggg gggacggctg ccttcggggg
     1201 ggacggggca gggcggggtt cggcttctgg cgtgtgaccg gcggctctag agcctctgct
     1261 aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct ggttattgtg
     1321 ctgtctcatc attttggcaa agaattcctc gagccaccat atggtgagca agggcgagga
     1381 ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt
     1441 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
     1501 gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc
     1561 ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta
     1621 cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
     1681 cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt
     1741 gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg
     1801 ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa
     1861 gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa
     1921 ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac
     1981 ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc
     2041 caccggcggc atggacgagc tgtacaaggg taccgagacc gacctcaaca gccaggaccg
     2101 taaagacctc gacaagttca ttaagttttt cgccctcaag accgtgcagg tgatcgtgca
     2161 ggcccggctg ggagagaaaa tctgcacacg cagctcctct tcacccaccg gttctgactg
     2221 gttcaacctg gctattaagg atatccctga agtgactcac gaagcaaaga aagcactcgc
     2281 tggacagctg ccagcagtgg gtcgttcaat gtgtgtcgag atctccctga agacatctga
     2341 aggagactca atggagctcg aaatttggtg cctggagatg aacgaaaagt gtgataaaga
     2401 gatcaaagtg agctacaccg tctataatcg tctgtccctg ctcctgaaat ctctcctggc
     2461 catcactcgg gtgacacctg cttaccgcct cagtaggaag cagggtcatg aatatgtgat
     2521 tctgtacagg atctatttcg gcgaggtcca gctctccgga ctgggtgaag gctttcagac
     2581 tgtgcgtgtc ggaaccgtgg gaactccagt cggcacaatt accctctctt gcgcataccg
     2641 tatcaatctg gccttcatgt caaccagaca gtttgagcgt actcccccta ttatgggaat
     2701 cattatcgac cacttcgtgg atagaccata ccccagtagc tcccctatgc atccatgcaa
     2761 ctatcgtaca gccggtgaag acaccggcgt gatctacccc agtgtcgagg atagccagga
     2821 agtgtgtacc acttccttct ctacctcacc accctcacag ctctcttcaa gtcgcctgag
     2881 ttatcagcct gccgctctcg gagtcggtag cgcagacctg gcctacccag tggtcttcgc
     2941 agcaggcctc aacgcaaccc acccacatca gctgatggtg ccaggaaagg agggtggagt
     3001 cccactggct cctaatcagc cagtgcacgg tactcaggct gaccaggaaa ggctggcaac
     3061 ttgcacacca tccgatagaa ctcattgtgc tgcaacaccc agctcctctg aggacaccga
     3121 aactgtgagc aactcaagtg agggtcgcgc ctccccacac gatgtcctgg aaaccatctt
     3181 cgtgagaaaa gtcggcgctt ttgtgaacaa gcccattaat caggtcacac tcacctctct
     3241 ggacatccct ttcgctatgt ttgcaccaaa gaacctcgag ctggaagaca ctgatcccat
     3301 ggtgaatcct ccagactcac ccgagacaga aagtcctctc cagggcagtc tgcacagcga
     3361 tggaagctcc ggtggctctt caggcaacac acatgacgat ttcgtcatga ttgacttcaa
     3421 acccgctttt tccaaggacg atatcctccc tatggatctg ggaaccttct acagggagtt
     3481 tcagaatccc cctcagctca gtagcctgag cattgacatc ggtgcccagt ccatggctga
     3541 ggacctcgat tctctgcctg aaaaactggc tgtgcacgag aagaacgtca gagaattcga
     3601 cgcatttgtg gagaccctgc agtgacgcca ttaacctgat gttctggggc aagcttacta
     3661 gtcctcagtg caggctgcct atcagaaggt ggtggctggt gtggccaatg ccctggctca
     3721 caaataccac tgagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct
     3781 tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa
     3841 ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa
     3901 tgagtatttg gtttagagtt tggcaacata tgcccatatg ctggctgcca tgaacaaagg
     3961 ttggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt ccttattcca
     4021 tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt tatttttttc
     4081 tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc tcctctcctg
     4141 actactccca gtcatagctg tccctcttct cttatggaga tccctcgacc tgcagcccaa
     4201 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     4261 cacacaacat acgagccgga agcataaagt gtaaagcctg ggtgcctaat gagtgagcta
     4321 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     4381 gcggatccgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     4441 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     4501 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     4561 tttggaggcc taggcttttg caaaaagcta acttgtttat tgcagcttat aatggttaca
     4621 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     4681 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccgctgc attaatgaat
     4741 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     4801 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     4861 aatacggtta tccacagaat cagggataac gcaggaaaga acatgtgagc aaaaggccag
     4921 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     4981 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     5041 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     5101 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     5161 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     5221 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     5281 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     5341 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     5401 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     5461 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     5521 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     5581 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     5641 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     5701 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     5761 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     5821 gagggcttac catctggccc cagtgctgca atgatacctc gagagccacg ctcaccggct
     5881 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     5941 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     6001 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     6061 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     6121 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     6181 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     6241 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     6301 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     6361 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     6421 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     6481 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     6541 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     6601 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     6661 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgggtcgaca
     6721 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
     6781 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     6841 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     6901 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     6961 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     7021 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     7081 catcgctatt accatggtc
//
