LOCUS       Exported                7442 bp ds-DNA     circular SYN 01-JUL-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7442)
  AUTHORS   eliasa55
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jul 8, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7442
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_binding    3..25
                     /label=LacO
     primer_bind     45..61
                     /label=SMP2196
     CDS             83..100
                     /codon_start=1
                     /label=6His
                     /translation="HHHHHH"
     primer_bind     83..100
                     /label=SMP3647
     primer_bind     complement(97..124)
                     /label=SMP3044
     primer_bind     189..208
                     /label=SMP50
     primer_bind     complement(189..208)
                     /label=SMP51
     primer_bind     complement(191..208)
                     /label=SMP3659
     primer_bind     214..233
                     /label=T7
     misc_binding    233..255
                     /label=LacO
     primer_bind     complement(254..299)
                     /label=Vector.REV
     primer_bind     275..321
                     /label=Fragment.FOR
     CDS             303..1693
                     /codon_start=1
                     /label=BCL2L13 1-465 (soluble)
                     /translation="ASSSTVPLGFHYETKYVVLSYLGLLSQEKLQEQHLSSPQGVQLDI
                     ASQSLDQEILLKVKTEIEEELKSLDKEISEAFTSTGFDRHTSPVFSPANPESSMEDCLA
                     HLGEKVSQELKEPLHKALQMLLSQPVTYQAFRECTLETTVHASGWNKILVPLVLLRQML
                     LELTRRGQEPLSALLQFGVTYLEDYSAEYIIQQGGWGTVFSLESEEEEYPGITAEDSND
                     IYILPSDNSGQVSPPESPTVTTSWQSESLPVSLSASQSWHTESLPVSLGPESWQQIAMD
                     PEEVKSLDSNGAGEKSENNSSNSDIVHVEKEEVPEGMEEAAVASVVLPARELQEALPEA
                     PAPLLPHITATSLLGTREPDTEVITVEKSSPATSLFVELDEEEVKAATTEPTEVEEVVP
                     ALEPTETLLSEKEINAREESLVEELSPASEKKPVPPSEGKSRLSPAGEMKPMPLSEGKS
                     ILLFG"
     primer_bind     complement(1669..1706)
                     /label=Fragment.REV
     primer_bind     1692..1710
                     /label=Vector.FOR
     CDS             1698..1715
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     1716..1740
                     /label=SMP2156
     misc_feature    1722..2372
                     /label=GST no Met
     primer_bind     complement(1762..1779)
                     /label=SMP2192
     primer_bind     complement(2018..2041)
                     /label=SMP1669
     primer_bind     2257..2278
                     /label=SMP358
     primer_bind     2302..2321
                     /label=GEX5-for
     primer_bind     2333..2350
                     /label=pETG-for
     primer_bind     2334..2353
                     /label=SMP3299
     CDS             2388..2432
                     /label=S-tag
     primer_bind     complement(2388..2404)
                     /label=SMP2284
     primer_bind     complement(2470..2489)
                     /label=T7term
     primer_bind     complement(2470..2488)
                     /label=SMP545
     primer_bind     complement(2511..2526)
                     /label=T7terbis
     primer_bind     complement(2531..2551)
                     /label=pGMP-rev
     rep_origin      2568..3023
                     /direction=RIGHT
                     /label=M13 origin
     rep_origin      complement(2573..3013)
                     /direction=LEFT
                     /label=F1 ori
     CDS             complement(3141..3800)
                     /label=AmpR
     primer_bind     complement(3188..3207)
                     /label=AmpStop
     primer_bind     3934..3953
                     /label=AmpStart
     promoter        complement(4041..4069)
                     /label=Amp prom
     rep_origin      4150..4778
                     /direction=RIGHT
                     /label=ColE1 origin
     primer_bind     5149..5168
                     /label=GEX3-rev
     primer_bind     5178..5196
                     /label=GEX3-rev-25
     primer_bind     5210..5229
                     /label=SMP3644
     primer_bind     complement(5955..5972)
                     /label=SMP2212
     CDS             complement(5956..6912)
                     /label=LacI
     primer_bind     7116..7134
                     /label=SMP52
     primer_bind     7281..7298
                     /label=SMP2285
     primer_bind     7364..7383
                     /label=SMP2293
     primer_bind     7380..7395
                     /label=SMP49
     primer_bind     7380..7394
                     /label=pET-up
     primer_bind     7408..7424
                     /label=SMP1677
     primer_bind     7415..7432
                     /label=T7probis
     primer_bind     join(7426..7442,1..3)
                     /label=T7
ORIGIN
        1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
       61 gagatatacc atgggcagca gccatcacca tcatcaccac agccaggatc cgaattcgag
      121 ctcggcgcgc ctgcaggtcg acaagcttgc ggccgcataa tgcttaagtc gaacagaaag
      181 taatcgtatt gtacacggcc gcataatcga aattaatacg actcactata ggggaattgt
      241 gagcggataa caattcccca tcttagtata ttagttaagt ataagaagga gatatacata
      301 tggcgtcctc ttctactgtg cctctgggat ttcactatga aacaaagtat gttgttctca
      361 gctacttggg actcctctct caagagaagc tgcaagagca acatctttcc tcaccccaag
      421 gggttcaact agatatagct tcacaatctc tggatcaaga aattttatta aaagttaaaa
      481 ctgaaattga agaagagcta aaatctctgg acaaagaaat ttctgaagcc ttcaccagca
      541 caggctttga ccgtcacact tctccagtgt tcagccctgc caatccagaa agctcaatgg
      601 aagactgctt ggcccatctt ggagaaaaag tgtcccagga actgaaagag cctctccata
      661 aagcattgca aatgctcctg agccagccag tgacatatca ggcatttcgg gaatgtacac
      721 tggagaccac agttcatgcc agcggctgga ataagatttt ggtgcctctg gttttgctac
      781 gacaaatgct tttggaattg acaagacgtg gtcaagaacc tttgagcgca ctgctgcagt
      841 ttggcgtgac atacctggag gactattcgg cagagtacat cattcagcaa ggtggctggg
      901 gcactgtgtt tagtcttgag tcagaggagg aggaataccc tggaatcact gcagaagata
      961 gcaatgacat ttacatcctg cccagcgaca actctggaca agtcagtccc ccagagtctc
     1021 caactgtgac cacttcctgg cagtctgaga gcttacctgt gtcactgtca gctagccaga
     1081 gttggcacac agaaagcctg ccagtgtcac taggccctga gtcctggcag cagattgcaa
     1141 tggatcctga agaagtgaaa agcttagaca gcaacggagc tggagagaag agtgagaaca
     1201 actcctctaa ttctgacatt gtgcacgtgg agaaagaaga ggtgcccgag ggcatggaag
     1261 aggctgctgt ggcttctgtg gtcttgccag cgcgggagct gcaagaggca cttcctgaag
     1321 ccccagctcc cttgcttcca catatcactg ccacctccct gctggggaca agggaacctg
     1381 acacagaagt gatcacagtt gagaaatcca gccctgctac atctctgttt gtagaacttg
     1441 atgaagaaga ggtgaaagca gcaacaactg aacctactga agtggaggag gtggtccccg
     1501 cactggaacc cacagaaacg ctgctgagtg agaaggagat aaacgcaagg gaagagagcc
     1561 ttgtggaaga gctgtcccct gccagcgaga agaagcccgt gccgccgtct gagggcaagt
     1621 ctagactgtc ccccgccggt gagatgaagc ccatgccgct gtctgagggc aagtctatac
     1681 tgctgtttgg aggatcactg gttccgcgtg gatccggcgg atcccctata ctaggttatt
     1741 ggaaaattaa gggccttgtg caacccactc gacttctttt ggaatatctt gaagaaaaat
     1801 atgaagagca tttgtatgag cgcgatgaag gtgataaatg gcgaaacaaa aagtttgaat
     1861 tgggtttgga gtttcccaat cttccttatt atattgatgg tgatgttaaa ttaacacagt
     1921 ctatggccat catacgttat atagctgaca agcacaacat gttgggtggt tgtccaaaag
     1981 agcgtgcaga gatttcaatg cttgaaggag cggttttgga tattagatac ggtgtttcga
     2041 gaattgcata tagtaaagac tttgaaactc tcaaagttga ttttcttagc aagctacctg
     2101 aaatgctgaa aatgttcgaa gatcgtttat gtcataaaac atatttaaat ggtgatcatg
     2161 taacccatcc tgacttcatg ttgtatgacg ctcttgatgt tgttttatac atggacccaa
     2221 tgtgcctgga tgcgttccca aaattagttt gttttaaaaa acgtattgaa gctatcccac
     2281 aaattgataa gtacttgaaa tccagcaagt atatagcatg gcctttgcag ggctggcaag
     2341 ccacgtttgg tggtggcgac catcctccaa aatagctcga gtctggtaaa gaaaccgctg
     2401 ctgcgaaatt tgaacgccag cacatggact cgtctactag cgcagcttaa ttaacctagg
     2461 ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga
     2521 ggggtttttt gctgaaagga ggaactatat ccggattggc gaatgggacg cgccctgtag
     2581 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     2641 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     2701 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     2761 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     2821 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     2881 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     2941 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     3001 caaaatatta acgtttacaa tttctggcgg cacgatggca tgagattatc aaaaaggatc
     3061 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     3121 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     3181 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     3241 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     3301 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     3361 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     3421 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     3481 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     3541 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     3601 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     3661 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     3721 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     3781 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     3841 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     3901 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     3961 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatcatga
     4021 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     4081 aaataaacaa ataggtcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     4141 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     4201 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     4261 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     4321 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     4381 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     4441 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     4501 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     4561 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     4621 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     4681 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     4741 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     4801 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     4861 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     4921 cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg
     4981 gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     5041 aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg
     5101 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     5161 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     5221 gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc
     5281 tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata
     5341 aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg
     5401 ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg
     5461 gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta
     5521 tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca
     5581 gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg
     5641 gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt
     5701 catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt
     5761 atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac
     5821 gacaggagca cgatcatgct agtcatgccc cgcgcccacc ggaaggagct gactgggttg
     5881 aaggctctca agggcatcgg tcgagatccc ggtgcctaat gagtgagcta acttacatta
     5941 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     6001 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg tggtttttct
     6061 tttcaccagt gagacgggca acagctgatt gcccttcacc gcctggccct gagagagttg
     6121 cagcaagcgg tccacgctgg tttgccccag caggcgaaaa tcctgtttga tggtggttaa
     6181 cggcgggata taacatgagc tgtcttcggt atcgtcgtat cccactaccg agatgtccgc
     6241 accaacgcgc agcccggact cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt
     6301 ggcaaccagc atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa
     6361 accggacatg gcactccagt cgccttcccg ttccgctatc ggctgaattt gattgcgagt
     6421 gagatattta tgccagccag ccagacgcag acgcgccgag acagaactta atgggcccgc
     6481 taacagcgcg atttgctggt gacccaatgc gaccagatgc tccacgccca gtcgcgtacc
     6541 gtcttcatgg gagaaaataa tactgttgat gggtgtctgg tcagagacat caagaaataa
     6601 cgccggaaca ttagtgcagg cagcttccac agcaatggca tcctggtcat ccagcggata
     6661 gttaatgatc agcccactga cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc
     6721 ttcgacgccg cttcgttcta ccatcgacac caccacgctg gcacccagtt gatcggcgcg
     6781 agatttaatc gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac
     6841 gccaatcagc aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt
     6901 cagctccgcc atcgccgctt ccactttttc ccgcgttttc gcagaaacgt ggctggcctg
     6961 gttcaccacg cgggaaacgg tctgataaga gacaccggca tactctgcga catcgtataa
     7021 cgttactggt ttcacattca ccaccctgaa ttgactctct tccgggcgct atcatgccat
     7081 accgcgaaag gttttgcgcc attcgatggt gtccgggatc tcgacgctct cccttatgcg
     7141 actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa
     7201 ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca
     7261 tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg
     7321 tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga
     7381 tgcgtccggc gtagaggatc gagatcgatc tcgatcccgc gaaattaata cgactcacta
     7441 ta
//
