LOCUS       Exported                6706 bp ds-DNA     circular SYN 03-JUL-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6706)
  AUTHORS   eliasa55
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jul 8, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6706
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     149..166
                     /label=SMP_2198
     primer_bind     177..196
                     /label=SMP_1565
     primer_bind     179..201
                     /label=SMP_357
     promoter        183..211
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     primer_bind     183..202
                     /label=SMP_3645
     primer_bind     187..204
                     /label=SMP_2193
     protein_bind    219..235
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(227..266)
                     /label=Vector.REV
     primer_bind     238..252
                     /label=SMP_993
     primer_bind     249..285
                     /label=Fragment.FOR
     CDS             258..914
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MASPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNK
                     KFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIR
                     YGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVV
                     LYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     258..285
                     /label=SMP4833
                     /note="gibson into pCAG with ClaI"
     primer_bind     258..285
                     /label=SMP4862
                     /note="for putting point mutants (round-the-horns of 
                     SMC2178) back into pCAGG vector (SMC1776 as template + ClaI
                     + XmaI)forward gibson primer (can be used with SMP4863, 
                     4864 and 4865)"
     primer_bind     258..276
                     /label=SMP2582
     regulatory      258..261
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     primer_bind     complement(304..321)
                     /label=SMP2192
     primer_bind     complement(560..583)
                     /label=SMP1669
     primer_bind     799..820
                     /label=SMP358
     primer_bind     844..863
                     /label=GEX5-for
     primer_bind     875..892
                     /label=pETG-for
     primer_bind     876..895
                     /label=SMP3299
     primer_bind     complement(894..920)
                     /label=SMP2863
     primer_bind     925..941
                     /label=SMP2555
     CDS             942..962
                     /codon_start=1
                     /label=TEV cleavage site
                     /note="TEV cleavage site"
                     /translation="ENLYFQG"
     CDS             969..1685
                     /codon_start=1
                     /label=mEGFP
                     /note="EGFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     969..988
                     /label=SMP2235
     primer_bind     969..988
                     /label=SMP3364
     primer_bind     972..989
                     /label=SMP2824
     primer_bind     complement(972..989)
                     /label=SMP3729
     primer_bind     975..992
                     /label=SMP2016
     primer_bind     complement(1011..1027)
                     /label=PEN1-737R
     primer_bind     complement(1014..1035)
                     /label=SMP3301
     primer_bind     complement(1016..1036)
                     /label=SMP919
     primer_bind     complement(1031..1049)
                     /label=SMP2564
     primer_bind     complement(1042..1062)
                     /label=SMP2562
     primer_bind     complement(1044..1062)
                     /label=EGFP-N-R+33
     primer_bind     complement(1077..1095)
                     /label=EGFP-N-rev
     primer_bind     complement(1085..1104)
                     /label=SMP2433
     primer_bind     complement(1100..1118)
                     /label=SMP3388
     primer_bind     1143..1163
                     /label=SMP2316
     primer_bind     1217..1236
                     /label=SMP1001
     primer_bind     1263..1282
                     /label=SMP1325
     primer_bind     complement(1273..1293)
                     /label=SMP2969
     primer_bind     complement(1330..1350)
                     /label=SMP2971
     primer_bind     complement(1333..1349)
                     /label=SMP2578
     primer_bind     complement(1363..1376)
                     /label=SMP2969
     primer_bind     1376..1394
                     /label=SMP444
     primer_bind     1386..1410
                     /label=SMP1666
     primer_bind     1440..1457
                     /label=SMP3040
     primer_bind     1440..1457
                     /label=SMP3104
     primer_bind     1445..1464
                     /label=SMP1330
     primer_bind     1472..1491
                     /label=SMP437
     primer_bind     complement(1472..1491)
                     /label=SMP471
     primer_bind     1523..1542
                     /label=SMP364
     primer_bind     1545..1554
                     /label=SMP4863
                     /note="for putting point mutants (round-the-horns of 
                     SMC2178) back into pCAGG vector (SMC1776 as template + ClaI
                     + XmaI)reverse gibson primer"
     primer_bind     1572..1604
                     /label=SMP295
     primer_bind     complement(1572..1604)
                     /label=SMP296
     primer_bind     1595..1613
                     /label=EGFP-C-F-31
     primer_bind     1604..1620
                     /label=EGFP-for
     primer_bind     1610..1628
                     /label=SMP564
     primer_bind     1622..1643
                     /label=SMP3300
     primer_bind     1626..1643
                     /label=EGFP-C-for
     primer_bind     complement(1658..1676)
                     /label=SMP2415
     primer_bind     complement(1665..1685)
                     /label=SMP2429
     primer_bind     1665..1685
                     /label=SMP3728
     primer_bind     complement(1665..1684)
                     /label=SMP2596
     CDS             1698..2678
                     /codon_start=1
                     /label=ATG13-IDR (191-517)
                     /note="ATG13"
                     /translation="INLAFAATAQFARAAPAAGAIIAAFVDRPYPSSSPMHPCNYRTAG
                     EDTGVIYPSVEDSQEVCTTSFSTSPPSQLSSSRLSYQPAALGVGSADLAYPVVFAAGLN
                     ATHPHQLMVPGKEGGVPLAPNQPVHGTQADQERLATCTPSDRTHCAATPSSSEDTETVS
                     NSSEGRASPHDVLETIFVRKVGAFVNKPINQVTLTSLDIPFAMFAPKNLELEDTDPMVN
                     PPDSPETESPLQGSLHSDGSSGGSSGNTHDDFVMIDFKPAFSKDDILPMDLGTFYREFQ
                     NPPQLSSLSIDIGAQSMAEDLDSLPEKLAVHEKNVREFDAFVETLQ"
     misc_feature    1713..1715
                     /label=M196A
                     /note="potentially abrogates WIPI2d interaction"
     misc_feature    1716..1718
                     /label=S197A
                     /note="potentially abrogates WIPI2d interaction"
     misc_feature    1722..1724
                     /label=R199A
                     /note="potentially abrogates WIPI2d interaction"
     misc_feature    1731..1733
                     /label=G202A
     misc_feature    1737..1739
                     /label=T204A
     misc_feature    1740..1742
                     /label=P205A
     misc_feature    1746..1748
                     /label=I207A
     misc_feature    1749..1751
                     /label=M208A
     misc_feature    1755..1757
                     /label=I210A
     misc_feature    1764..1766
                     /label=D213A
     misc_feature    1767..1769
                     /label=H214A
     primer_bind     1779..1796
                     /label=SMP2547
     primer_bind     complement(1916..1932)
                     /label=SMP2554
     primer_bind     complement(2667..2690)
                     /label=SMP4834
                     /note="reverse gibson"
     primer_bind     complement(2667..2690)
                     /label=rvelias
     primer_bind     complement(2672..2698)
                     /label=Fragment.REV
     primer_bind     complement(2682..2698)
                     /label=Fragment.REVa
     primer_bind     2684..2703
                     /label=Vector.FOR
     primer_bind     complement(2723..2742)
                     /label=SMP_3644
     primer_bind     complement(2756..2774)
                     
     primer_bind     complement(2784..2803)
                     
     primer_bind     3008..3028
                     /label=SMP_646
     primer_bind     complement(3008..3028)
                     /label=SMP_654
     promoter        3009..3113
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3114..3974
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4145..4733
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4525..4542
                     /label=SMP_4077
     promoter        4977..5054
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             5055..6137
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(5322..5339)
                     /label=SMP_4079
     primer_bind     6118..6135
                     /label=SMP_2212
     primer_bind     complement(6120..6137)
                     /label=SMP_4078
     protein_bind    6150..6171
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6186..6216
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6224..6240
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(6232..6257)
                     /label=Vector.REV
     primer_bind     6243..6262
                     /label=SMP_993
     primer_bind     6248..6264
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6248..6264
                     /label=SMP_1770
     CDS             join(6260..6706,1..81)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVGITLSTARCTNASGVR
                     QPSEAVVWLCRS"
     primer_bind     complement(6280..6296)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6281..6296)
                     /label=SMP_1771
     primer_bind     complement(6284..6305)
                     /label=SMP_469
     primer_bind     complement(6320..6340)
                     /label=SMP_3886
     primer_bind     complement(6326..6346)
                     /label=SMP_3888
     primer_bind     complement(6334..6353)
                     /label=SMP_3890
ORIGIN
        1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
       61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
      121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
      181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
      241 cacaggaaac agtattcatg gcatccccta tactaggtta ttggaaaatt aagggccttg
      301 tgcaacccac tcgacttctt ttggaatatc ttgaagaaaa atatgaagag catttgtatg
      361 agcgcgatga aggtgataaa tggcgaaaca aaaagtttga attgggtttg gagtttccca
      421 atcttcctta ttatattgat ggtgatgtta aattaacaca gtctatggcc atcatacgtt
      481 atatagctga caagcacaac atgttgggtg gttgtccaaa agagcgtgca gagatttcaa
      541 tgcttgaagg agcggttttg gatattagat acggtgtttc gagaattgca tatagtaaag
      601 actttgaaac tctcaaagtt gattttctta gcaagctacc tgaaatgctg aaaatgttcg
      661 aagatcgttt atgtcataaa acatatttaa atggtgatca tgtaacccat cctgacttca
      721 tgttgtatga cgctcttgat gttgttttat acatggaccc aatgtgcctg gatgcgttcc
      781 caaaattagt ttgttttaaa aaacgtattg aagctatccc acaaattgat aagtacttga
      841 aatccagcaa gtatatagca tggcctttgc agggctggca agccacgttt ggtggtggcg
      901 accatcctcc aaaatcggat gattacgata tcccaacgac cgaaaacctg tattttcagg
      961 gtggtaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     1021 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     1081 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     1141 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
     1201 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     1261 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     1321 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     1381 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     1441 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     1501 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     1561 acaaccacta cctgagcacc cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc
     1621 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     1681 acaagggtac cggtggtatc aatctggcct tcgcggcaac cgcacagttt gcgcgtgctg
     1741 cccctgctgc gggagccatt atcgccgcct tcgtggatag accatacccc agtagctccc
     1801 ctatgcatcc atgcaactat cgtacagccg gtgaagacac cggcgtgatc taccccagtg
     1861 tcgaggatag ccaggaagtg tgtaccactt ccttctctac ctcaccaccc tcacagctct
     1921 cttcaagtcg cctgagttat cagcctgccg ctctcggagt cggtagcgca gacctggcct
     1981 acccagtggt cttcgcagca ggcctcaacg caacccaccc acatcagctg atggtgccag
     2041 gaaaggaggg tggagtccca ctggctccta atcagccagt gcacggtact caggctgacc
     2101 aggaaaggct ggcaacttgc acaccatccg atagaactca ttgtgctgca acacccagct
     2161 cctctgagga caccgaaact gtgagcaact caagtgaggg tcgcgcctcc ccacacgatg
     2221 tcctggaaac catcttcgtg agaaaagtcg gcgcttttgt gaacaagccc attaatcagg
     2281 tcacactcac ctctctggac atccctttcg ctatgtttgc accaaagaac ctcgagctgg
     2341 aagacactga tcccatggtg aatcctccag actcacccga gacagaaagt cctctccagg
     2401 gcagtctgca cagcgatgga agctccggtg gctcttcagg caacacacat gacgatttcg
     2461 tcatgattga cttcaaaccc gctttttcca aggacgatat cctccctatg gatctgggaa
     2521 ccttctacag ggagtttcag aatccccctc agctcagtag cctgagcatt gacatcggtg
     2581 cccagtccat ggctgaggac ctcgattctc tgcctgaaaa actggctgtg cacgagaaga
     2641 acgtcagaga attcgacgca tttgtggaga ccctgcagtg acccgggtga ctcgagcggc
     2701 cgcatcgtga ctgactgacg atctgcctcg cgcgtttcgg tgatgacggt gaaaacctct
     2761 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     2821 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt
     2881 cacgtagcga tagcggagtg tataattctt gaagacgaaa gggcctcgtg atacgcctat
     2941 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     3001 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     3061 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     3121 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     3181 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     3241 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     3301 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg
     3361 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     3421 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     3481 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     3541 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     3601 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag
     3661 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     3721 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     3781 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     3841 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     3901 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     3961 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     4021 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     4081 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     4141 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     4201 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     4261 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     4321 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     4381 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     4441 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     4501 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     4561 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     4621 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     4681 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     4741 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     4801 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     4861 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     4921 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata aattccgaca
     4981 ccatcgaatg gtgcaaaacc tttcgcggta tggcatgata gcgcccggaa gagagtcaat
     5041 tcagggtggt gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat gccggtgtct
     5101 cttatcagac cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg
     5161 aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg gcacaacaac
     5221 tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc
     5281 cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc agcgtggtgg
     5341 tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg
     5401 cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat gccattgctg
     5461 tggaagctgc ctgcactaat gttccggcgt tatttcttga tgtctctgac cagacaccca
     5521 tcaacagtat tattttctcc catgaagacg gtacgcgact gggcgtggag catctggtcg
     5581 cattgggtca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc
     5641 tgcgtctggc tggctggcat aaatatctca ctcgcaatca aattcagccg atagcggaac
     5701 gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg
     5761 gcatcgttcc cactgcgatg ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg
     5821 ccattaccga gtccgggctg cgcgttggtg cggatatctc ggtagtggga tacgacgata
     5881 ccgaagacag ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc
     5941 tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca
     6001 atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa
     6061 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     6121 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     6181 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     6241 tttcacacag gaaacagcta tgaccatgat tacggattca ctggccgtcg ttttacaacg
     6301 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     6361 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     6421 cctgaatggc gaatggcgct ttgcctggtt tccggcacca gaagcggtgc cggaaagctg
     6481 gctggagtgc gatcttcctg aggccgatac tgtcgtcgtc ccctcaaact ggcagatgca
     6541 cggttacgat gcgcccatct acaccaacgt aacctatccc attacggtca atccgccgtt
     6601 tgttcccacg gagaatccga cgggttgtta ctcgctcaca tttaatgttg atgaaagctg
     6661 gctacaggaa ggccagacgc gaattatttt tgatggcgtt ggaatt
//
