LOCUS       pET50b_SynPspA          7339 bp ds-DNA     circular     30-AUG-2022
DEFINITION  .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
COMMENT     Imported using the Genbank importer. File name: pET-50b(+)
            synPspA.seq synthetic circular DNA ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             10..27
                     /label="thrombin site"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             28..72
                     /label="S-Tag"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     terminator      167..214
                     /label="T7 terminator"
                     /ApEinfo_revcolor="#9d1b1c"
                     /ApEinfo_fwdcolor="#9d1b1c"
     rep_origin      251..706
                     /label="f1 ori"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             complement(799..1614)
                     /label="KanR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     rep_origin      1736..2324
                     /label="ori"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2510..2652
                     /label="bom"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             complement(2754..2945)
                     /label="rop"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             complement(3517..4599)
                     /label="lacI"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     promoter        complement(4600..4677)
                     /label="lacI promoter"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_fwdcolor="#346ee0"
     promoter        4990..5008
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_fwdcolor="#346ee0"
     protein_bind    5009..5033
                     /label="lac operator"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     RBS             5048..5070
                     /label="efficient ribosome binding site frombacteriophage T7 gene 10 (Olins and Rangwala, 1989)"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             5078..7339
                     /label="Translation 5078-7339"
                     /translation="MGSSHHHHHHSSMNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEIDVRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTFDRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEAVILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGEEVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLWDDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGWELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKELLEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAARGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEATSGSGHHHHHHSAALEVLFQGPGMELFNRVGRVLKSQLTHWQQQQEAPEDLLERLLGEMELELIELRRALAQTIATFKSTERQRDAQQLIAQRWYEKAQAALDRGNEQLAREALGQRQSYQSHTEALGKSLGEQRALVEQVRGQLQKLERKYLELKSQKNLYLARLKSAIAAQKIEEIAGNLDNASASSLFERIETKILELEAERELLNPPPSPLDKKFEQWEEQQAVEATLAAMKARRSLPPPSS*"
     CDS             5078..6664
                     /label="Translation 5078-6664"
                     /translation="MGSSHHHHHHSSMNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEIDVRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTFDRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEAVILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGEEVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLWDDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGWELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKELLEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAARGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEATSGSGHHHHHHSAALEVLFQGP"
     CDS             5090..5107
                     /label="6xHis"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             5114..6598
                     /label="NusA"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             6614..6631
                     /label="6xHis"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             6641..6664
                     /label="HRV 3C site"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          6661..6684
                     /label="SynPspA_SapI_FW"
                     /note="sequence: GGTGGTTGCTCTTCCAACatggaactatttaataggg"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    6668..7339
                     /label="synPspA"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(7325..7339)
                     /label="SynPspA_SpeI_REV"
                     /note="sequence: GGTGGTACTAGTttacgagcttggcgg"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
ORIGIN
        1 gagctcgctc tggtgccacg cggtagtaaa gaaaccgctg ctgctaaatt cgaacgccag
       61 cacatggaca gctctacttc tgctgctctc gaggcttaat taacctaggc tgctaaacaa
      121 agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct
      181 tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggatt
      241 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
      301 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
      361 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
      421 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
      481 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
      541 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
      601 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
      661 caaaaattta acgcgaattt taacaaaata ttaacgttta caatttcagg tggcactttt
      721 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
      781 ccgctcatga attaattctt agaaaaactc atcgagcatc aaatgaaact gcaatttatt
      841 catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa
      901 ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg
      961 tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa
     1021 atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca
     1081 gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc
     1141 gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcactgt taaaaggaca
     1201 attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt
     1261 ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttgccgg ggatcgcagt
     1321 ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat
     1381 aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc
     1441 tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt
     1501 cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat
     1561 gttggaattt aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc tcataacacc
     1621 ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgaccaaa atcccttaac
     1681 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     1741 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     1801 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     1861 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     1921 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     1981 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     2041 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     2101 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     2161 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     2221 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     2281 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     2341 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     2401 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     2461 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt
     2521 attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac
     2581 aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg
     2641 gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     2701 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     2761 ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg
     2821 tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga
     2881 agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg
     2941 gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa
     3001 cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt
     3061 tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt
     3121 caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct
     3181 gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac
     3241 gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag
     3301 cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc
     3361 cgccagccta gccgggtcct caacgacagg agcacgatca tgctagtcat gccccgcgcc
     3421 caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc
     3481 taatgagtga gctaacttac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     3541 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     3601 attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt
     3661 caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg
     3721 aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtctt cggtatcgtc
     3781 gtatcccact accgagatgt ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat
     3841 tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt
     3901 cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc
     3961 tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc
     4021 cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag
     4081 atgctccacg cccagtcgcg taccgtcttc atgggagaaa ataatactgt tgatgggtgt
     4141 ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat
     4201 ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag
     4261 attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac
     4321 gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg
     4381 cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg
     4441 tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt
     4501 tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc
     4561 ggcatactct gcgacatcgt ataacgttac tggtttcaca ttcaccaccc tgaattgact
     4621 ctcttccggg cgctatcatg ccataccgcg aaaggttttg cgccattcga tggtgtccgg
     4681 gatctcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga
     4741 ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc
     4801 ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt
     4861 ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg
     4921 tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc gatctcgatc
     4981 ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag
     5041 aaataatttt gtttaacttt aagaaggaga tatacatatg ggcagcagcc atcaccacca
     5101 tcaccattct agtatgaaca aagaaatttt ggctgtagtt gaagccgtat ccaatgaaaa
     5161 ggcgctacct cgcgagaaga ttttcgaagc attggaaagc gcgctggcga cagcaacaaa
     5221 gaaaaaatat gaacaagaga tcgacgtccg cgtacagatc gatcgcaaaa gcggtgattt
     5281 tgacactttc cgtcgctggt tagttgttga tgaagtcacc cagccgacca aggaaatcac
     5341 ccttgaagcc gcacgttatg aagatgaaag cctgaacctg ggcgattacg ttgaagatca
     5401 gattgagtct gttacctttg accgtatcac tacccagacg gcaaaacagg ttatcgtgca
     5461 gaaagtgcgt gaagccgaac gtgcgatggt ggttgatcag ttccgtgaac acgaaggtga
     5521 aatcatcacc ggcgtggtga aaaaagtaaa ccgcgacaac atctctctgg atctgggcaa
     5581 caacgctgaa gccgtgatcc tgcgcgaaga tatgctgccg cgtgaaaact tccgccctgg
     5641 cgaccgcgtt cgtggcgtgc tctattccgt tcgcccggaa gcgcgtggcg cgcaactgtt
     5701 cgtcactcgt tccaagccgg aaatgctgat cgaactgttc cgtattgaag tgccagaaat
     5761 cggcgaagaa gtgattgaaa ttaaagcagc ggctcgcgat ccgggttctc gtgcgaaaat
     5821 cgcggtgaaa accaacgata aacgtatcga tccggtaggt gcttgcgtag gtatgcgtgg
     5881 cgcgcgtgtt caggcggtgt ctactgaact gggtggcgag cgtatcgata tcgtcctgtg
     5941 ggatgataac ccggcgcagt tcgtgattaa cgcaatggca ccggcagacg ttgcttctat
     6001 cgtggtggat gaagataaac acaccatgga catcgccgtt gaagccggta atctggcgca
     6061 ggcgattggc cgtaacggtc agaacgtgcg tctggcttcg caactgagcg gttgggaact
     6121 caacgtgatg accgttgacg acctgcaagc taagcatcag gcggaagcgc acgcagcgat
     6181 cgacaccttc accaaatatc tcgacatcga cgaagacttc gcgactgttc tggtagaaga
     6241 aggcttctcg acgctggaag aattggccta tgtgccgatg aaagagctgt tggaaatcga
     6301 aggccttgat gagccgaccg ttgaagcact gcgcgagcgt gctaaaaatg cactggccac
     6361 cattgcacag gcccaggaag aaagcctcgg tgataacaaa ccggctgacg atctgctgaa
     6421 ccttgaaggg gtagatcgtg atttggcatt caaactggcc gcccgtggcg tttgtacgct
     6481 ggaagatctc gccgaacagg gcattgatga tctggctgat atcgaagggt tgaccgacga
     6541 aaaagccgga gcactgatta tggctgcccg taatatttgc tggttcggtg acgaagcgac
     6601 tagtggttct ggtcatcacc atcaccatca ctccgcggct cttgaagtcc tctttcaggg
     6661 acccgggatg gaactattta atagggttgg aagagtattg aagtcccaat tgacccattg
     6721 gcagcagcag caagaagctc cggaagatct gttggagcgc ctgctgggcg aaatggaatt
     6781 ggagctgatc gagctgcgtc gcgcactggc gcagaccatt gcgacgttta aaagcactga
     6841 gcgccaacgt gatgcgcaac agctgattgc ccaacgttgg tacgaaaagg cgcaggcggc
     6901 gttggaccgt ggtaatgagc aactagctcg tgaagcgctg ggtcagcgtc agagctatca
     6961 gtcccacacc gaagcgctcg gcaaatctct gggtgaacaa cgtgcattgg tcgagcaggt
     7021 tagaggccag ctgcaaaagc tggagcgtaa atacctggag cttaagagcc agaagaacct
     7081 ttatctggcc cgcctgaagt cagcaattgc tgcgcagaag atcgaggaga tcgcgggtaa
     7141 cctggacaac gcgagcgcaa gctctctgtt cgaacggatt gaaaccaaaa tcctggagtt
     7201 agaggccgaa cgtgaactgt tgaatccgcc tccgtccccg ctggacaaaa aattcgagca
     7261 gtgggaagag caacaagctg tggaagcaac cctggctgcc atgaaagcgc gccgtagctt
     7321 accgccgcca agctcgtaa
//