LOCUS       pET50delCT_Vipp1        5990 bp ds-DNA     circular     10-JUL-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..66
                     /label="H0"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(47..66)
                     /label="pRSET6a IM30 wt REV"
                     /note="sequence: TAAGAAGGAGATATACATatgggattatttgaccgttt"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          67..97
                     /label="pET50delCT SynPspA FWD"
                     /note="sequence: acggtcaaataatcccatATGTATATCTCCTTCTTAAAGTTAAACAAAA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     RBS             75..80
                     /label="RBS"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     protein_bind    111..135
                     /label="lac operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     promoter        complement(136..154)
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        467..544
                     /label="lacI promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             545..1627
                     /label="lacI"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     CDS             2199..2390
                     /label="rop"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     rep_origin      complement(2820..3408)
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             3530..4345
                     /label="KanR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     rep_origin      complement(4438..4893)
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     terminator      4930..4977
                     /label="T7 terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(5072..5116)
                     /label="S-Tag"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             complement(5117..5134)
                     /label="thrombin site"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    5138..5204
                     /label="MCS"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             complement(5197..66)
                     /label="Translation 66-5197"
                     /translation="MGLFDRLGRVVRANLNDLVSKAEDPEKVLEQAVIDMQEDLVQLRQAVARTIAEEKRTEQRLNQDTQEAKKWEDRAKLALTNGEENLAREALARKKSLTDTAAAYQTQLAQQRTMSENLRRNLAALEAKISEAKTKKNMLQARAKAAKANAELQQTLGGLGTSSATSAFERMENKVLDMEATSQAAGELAGFGIENQFAQLEASSGVEDELAALKASMAGGALPGTSAATPQLEAAPVDSSVPANNASQDDAVIDQELDDLRRRLNNLAALEVLFQGPHHHHHH*GS"
     misc_feature    complement(5226..5255)
                     /label="3C site"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             complement(5229..66)
                     /label="Translation 66-5229"
                     /translation="MGLFDRLGRVVRANLNDLVSKAEDPEKVLEQAVIDMQEDLVQLRQAVARTIAEEKRTEQRLNQDTQEAKKWEDRAKLALTNGEENLAREALARKKSLTDTAAAYQTQLAQQRTMSENLRRNLAALEAKISEAKTKKNMLQARAKAAKANAELQQTLGGLGTSSATSAFERMENKVLDMEATSQAAGELAGFGIENQFAQLEASSGVEDELAALKASMAGGALPGTSAATPQLEAAPVDSSVPANNASQDDAVIDQELDDLRRRLNNLAALEVLFQG"
     primer          complement(5236..5255)
                     /label="pET50delCT SynPspA REV"
                     /note="sequence: gtcgtcggttaaatGCGGCTCTTGAAGTCCTCTT"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(5256..66)
                     /label="Vipp1"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          5256..5275
                     /label="pRSET6a IM30 wt FWD"
                     /note="sequence: AAGAGGACTTCAAGcagattatttaaccgacgac"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
ORIGIN
        1 agctttactg acgaggtcgt tgaggttagc ccggacgacg cggcctaaac ggtcaaataa
       61 tcccatATGT ATATCTCCTT CTTAAAGTTA AACAAAATTA TTTCTAGAGG GGAATTGTTA
      121 TCCGCTCACA ATTCCCCTAT AGTGAGTCGT ATTAATTTCG CGGGATCGAG ATCGATCTCG
      181 ATCCTCTACG CCGGACGCAT CGTGGCCGGC ATCACCGGCG CCACAGGTGC GGTTGCTGGC
      241 GCCTATATCG CCGACATCAC CGATGGGGAA GATCGGGCTC GCCACTTCGG GCTCATGAGC
      301 GCTTGTTTCG GCGTGGGTAT GGTGGCAGGC CCCGTGGCCG GGGGACTGTT GGGCGCCATC
      361 TCCTTGCATG CACCATTCCT TGCGGCGGCG GTGCTCAACG GCCTCAACCT ACTACTGGGC
      421 TGCTTCCTAA TGCAGGAGTC GCATAAGGGA GAGCGTCGAG ATCCCGGACA CCATCGAATG
      481 GCGCAAAACC TTTCGCGGTA TGGCATGATA GCGCCCGGAA GAGAGTCAAT TCAGGGTGGT
      541 GAATGTGAAA CCAGTAACGT TATACGATGT CGCAGAGTAT GCCGGTGTCT CTTATCAGAC
      601 CGTTTCCCGC GTGGTGAACC AGGCCAGCCA CGTTTCTGCG AAAACGCGGG AAAAAGTGGA
      661 AGCGGCGATG GCGGAGCTGA ATTACATTCC CAACCGCGTG GCACAACAAC TGGCGGGCAA
      721 ACAGTCGTTG CTGATTGGCG TTGCCACCTC CAGTCTGGCC CTGCACGCGC CGTCGCAAAT
      781 TGTCGCGGCG ATTAAATCTC GCGCCGATCA ACTGGGTGCC AGCGTGGTGG TGTCGATGGT
      841 AGAACGAAGC GGCGTCGAAG CCTGTAAAGC GGCGGTGCAC AATCTTCTCG CGCAACGCGT
      901 CAGTGGGCTG ATCATTAACT ATCCGCTGGA TGACCAGGAT GCCATTGCTG TGGAAGCTGC
      961 CTGCACTAAT GTTCCGGCGT TATTTCTTGA TGTCTCTGAC CAGACACCCA TCAACAGTAT
     1021 TATTTTCTCC CATGAAGACG GTACGCGACT GGGCGTGGAG CATCTGGTCG CATTGGGTCA
     1081 CCAGCAAATC GCGCTGTTAG CGGGCCCATT AAGTTCTGTC TCGGCGCGTC TGCGTCTGGC
     1141 TGGCTGGCAT AAATATCTCA CTCGCAATCA AATTCAGCCG ATAGCGGAAC GGGAAGGCGA
     1201 CTGGAGTGCC ATGTCCGGTT TTCAACAAAC CATGCAAATG CTGAATGAGG GCATCGTTCC
     1261 CACTGCGATG CTGGTTGCCA ACGATCAGAT GGCGCTGGGC GCAATGCGCG CCATTACCGA
     1321 GTCCGGGCTG CGCGTTGGTG CGGACATCTC GGTAGTGGGA TACGACGATA CCGAAGACAG
     1381 CTCATGTTAT ATCCCGCCGT TAACCACCAT CAAACAGGAT TTTCGCCTGC TGGGGCAAAC
     1441 CAGCGTGGAC CGCTTGCTGC AACTCTCTCA GGGCCAGGCG GTGAAGGGCA ATCAGCTGTT
     1501 GCCCGTCTCA CTGGTGAAAA GAAAAACCAC CCTGGCGCCC AATACGCAAA CCGCCTCTCC
     1561 CCGCGCGTTG GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG
     1621 GCAGTGAGCG CAACGCAATT AATGTAAGTT AGCTCACTCA TTAGGCACCG GGATCTCGAC
     1681 CGATGCCCTT GAGAGCCTTC AACCCAGTCA GCTCCTTCCG GTGGGCGCGG GGCATGACTA
     1741 GCATGATCGT GCTCCTGTCG TTGAGGACCC GGCTAGGCTG GCGGGGTTGC CTTACTGGTT
     1801 AGCAGAATGA ATCACCGATA CGCGAGCGAA CGTGAAGCGA CTGCTGCTGC AAAACGTCTG
     1861 CGACCTGAGC AACAACATGA ATGGTCTTCG GTTTCCGTGT TTCGTAAAGT CTGGAAACGC
     1921 GGAAGTCAGC GCCCTGCACC ATTATGTTCC GGATCTGCAT CGCAGGATGC TGCTGGCTAC
     1981 CCTGTGGAAC ACCTACATCT GTATTAACGA AGCGCTGGCA TTGACCCTGA GTGATTTTTC
     2041 TCTGGTCCCG CCGCATCCAT ACCGCCAGTT GTTTACCCTC ACAACGTTCC AGTAACCGGG
     2101 CATGTTCATC ATCAGTAACC CGTATCGTGA GCATCCTCTC TCGTTTCATC GGTATCATTA
     2161 CCCCCATGAA CAGAAATCCC CCTTACACGG AGGCATCAGT GACCAAACAG GAAAAAACCG
     2221 CCCTTAACAT GGCCCGCTTT ATCAGAAGCC AGACATTAAC GCTTCTGGAG AAACTCAACG
     2281 AGCTGGACGC GGATGAACAG GCAGACATCT GTGAATCGCT TCACGACCAC GCTGATGAGC
     2341 TTTACCGCAG CTGCCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC
     2401 TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG
     2461 GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCGCAGCCAT GACCCAGTCA CGTAGCGATA
     2521 GCGGAGTGTA TACTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA GAGTGCACCA
     2581 TATATGCGGT GTGAAATACC GCACAGATGC GTAAGGAGAA AATACCGCAT CAGGCGCTCT
     2641 TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA
     2701 GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC
     2761 ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT
     2821 TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG
     2881 CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC
     2941 TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC
     3001 GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC
     3061 AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC
     3121 TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT
     3181 AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT
     3241 AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC
     3301 TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT
     3361 TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG
     3421 ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC
     3481 ATGAACAATA AAACTGTCTG CTTACATAAA CAGTAATACA AGGGGTGTTA TGAGCCATAT
     3541 TCAACGGGAA ACGTCTTGCT CTAGGCCGCG ATTAAATTCC AACATGGATG CTGATTTATA
     3601 TGGGTATAAA TGGGCTCGCG ATAATGTCGG GCAATCAGGT GCGACAATCT ATCGATTGTA
     3661 TGGGAAGCCC GATGCGCCAG AGTTGTTTCT GAAACATGGC AAAGGTAGCG TTGCCAATGA
     3721 TGTTACAGAT GAGATGGTCA GACTAAACTG GCTGACGGAA TTTATGCCTC TTCCGACCAT
     3781 CAAGCATTTT ATCCGTACTC CTGATGATGC ATGGTTACTC ACCACTGCGA TCCCCGGCAA
     3841 AACAGCATTC CAGGTATTAG AAGAATATCC TGATTCAGGT GAAAATATTG TTGATGCGCT
     3901 GGCAGTGTTC CTGCGCCGGT TGCATTCGAT TCCTGTTTGT AATTGTCCTT TTAACAGTGA
     3961 TCGCGTATTT CGTCTCGCTC AGGCGCAATC ACGAATGAAT AACGGTTTGG TTGATGCGAG
     4021 TGATTTTGAT GACGAGCGTA ATGGCTGGCC TGTTGAACAA GTCTGGAAAG AAATGCATAA
     4081 ACTTTTGCCA TTCTCACCGG ATTCAGTCGT CACTCATGGT GATTTCTCAC TTGATAACCT
     4141 TATTTTTGAC GAGGGGAAAT TAATAGGTTG TATTGATGTT GGACGAGTCG GAATCGCAGA
     4201 CCGATACCAG GATCTTGCCA TCCTATGGAA CTGCCTCGGT GAGTTTTCTC CTTCATTACA
     4261 GAAACGGCTT TTTCAAAAAT ATGGTATTGA TAATCCTGAT ATGAATAAAT TGCAGTTTCA
     4321 TTTGATGCTC GATGAGTTTT TCTAAGAATT AATTCATGAG CGGATACATA TTTGAATGTA
     4381 TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGAAA
     4441 TTGTAAACGT TAATATTTTG TTAAAATTCG CGTTAAATTT TTGTTAAATC AGCTCATTTT
     4501 TTAACCAATA GGCCGAAATC GGCAAAATCC CTTATAAATC AAAAGAATAG ACCGAGATAG
     4561 GGTTGAGTGT TGTTCCAGTT TGGAACAAGA GTCCACTATT AAAGAACGTG GACTCCAACG
     4621 TCAAAGGGCG AAAAACCGTC TATCAGGGCG ATGGCCCACT ACGTGAACCA TCACCCTAAT
     4681 CAAGTTTTTT GGGGTCGAGG TGCCGTAAAG CACTAAATCG GAACCCTAAA GGGAGCCCCC
     4741 GATTTAGAGC TTGACGGGGA AAGCCGGCGA ACGTGGCGAG AAAGGAAGGG AAGAAAGCGA
     4801 AAGGAGCGGG CGCTAGGGCG CTGGCAAGTG TAGCGGTCAC GCTGCGCGTA ACCACCACAC
     4861 CCGCCGCGCT TAATGCGCCG CTACAGGGCG CGTCCCATTC GCCAATCCGG ATATAGTTCC
     4921 TCCTTTCAGC AAAAAACCCC TCAAGACCCG TTTAGAGGCC CCAAGGGGTT ATGCTAGTTA
     4981 TTGCTCAGCG GTGGCAGCAG CCAACTCAGC TTCCTTTCGG GCTTTGTTTA GCAGCCTAGG
     5041 TTAATTAAGC CTCGAGAGCA GCAGAAGTAG AGCTGTCCAT GTGCTGGCGT TCGAATTTAG
     5101 CAGCAGCGGT TTCTTTACTA CCGCGTGGCA CCAGAGCGAG CTCTGCGGCC GCAAGCTTGT
     5161 CGACGGACGT CGGGCGCGCC AAGGCCTGTA CAGAATTCGG ATCCttaATG GTGATGGTGG
     5221 TGATGGGGTC CCTGAAAGAG GACTTCAAGA GCCGCcagat tatttaaccg acgacgtagg
     5281 tcgtccaatt cctggtcaat caccgcatca tcttgactgg cattattagc tggtactgag
     5341 gaatcaacag gagccgcttc gagttggggc gtagcggcag aggttcccgg taatgctcca
     5401 ccggccatgg aagctttcag ggcggccaac tcgtcttcta ccccactgct ggcttccaac
     5461 tgggcaaact ggttctcgat gccaaagccg gctaactccc ctgccgcttg ggaagtggct
     5521 tccatatcca gtaccttgtt ctccatccgt tcaaaagcac tagtagcact gctagtacct
     5581 aagcccccga gggtttgctg cagttcagca ttagccttgg ccgctttggc cctggcctgc
     5641 aacatatttt tcttggtttt agcttcggaa atttttgctt ctagagccgc gaggttgcga
     5701 cggagattct ccgacatggt cctctgttgg gctagctggg tttggtacgc cgccgccgta
     5761 tctgtcagac tttttttgcg ggccagggct tccctagcca aattttcttc cccattggtg
     5821 agggctaatt ttgcccggtc ttcccatttc ttggcttcct gggtgtcttg gttaagacgt
     5881 tgctctgttc gtttttcttc ggcgatcgtg cgggcaacgg cctggcggag ttgcaccagg
     5941 tcttcctgca tatcgatcac ggcctgttcc agaacttttt ctggatcttc
//