LOCUS       pET50delCT_Vipp1_H1-6   5924 bp ds-DNA     circular     10-JUL-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             160..975
                     /label="KanR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     rep_origin      complement(1068..1523)
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     terminator      1560..1607
                     /label="T7 terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(1702..1746)
                     /label="S-Tag"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             complement(1747..1764)
                     /label="thrombin site"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1768..1834
                     /label="MCS"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             complement(1838..2620)
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MDPEKVLEQAVIDMQEDLVQLRQAVARTIAEEKRTEQRLNQDTQEAKKWEDRAKLALTNGEENLAREALARKKSLTDTAAAYQTQLAQQRTMSENLRRNLAALEAKISEAKTKKNMLQARAKAAKANAELQQTLGGLGTSSATSAFERMENKVLDMEATSQAAGELAGFGIENQFAQLEASSGVEDELAALKASMAGGALPGTSAATPQLEAAPVDSSVPANNASQDDAVIDQELDDLRRRLNNLAALEVLFQGPHHHHHH"
     misc_feature    complement(1856..1885)
                     /label="3C site"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(1886..2617)
                     /label="Vipp1"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          complement(2599..2617)
                     /label="Q5_Vipp1_delH0_R"
                     /note="sequence: gatccagaaaaagttctgg"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          2618..2641
                     /label="Q5_Vipp1_delH0_F"
                     /note="sequence: catATGTATATCTCCTTCTTAAAG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     RBS             2629..2634
                     /label="RBS"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     protein_bind    2665..2689
                     /label="lac operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     promoter        complement(2690..2708)
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        3021..3098
                     /label="lacI promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             3099..4181
                     /label="lacI"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     CDS             4753..4944
                     /label="rop"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     rep_origin      complement(5374..38)
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA
       61 CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAACAATA
      121 AAACTGTCTG CTTACATAAA CAGTAATACA AGGGGTGTTA TGAGCCATAT TCAACGGGAA
      181 ACGTCTTGCT CTAGGCCGCG ATTAAATTCC AACATGGATG CTGATTTATA TGGGTATAAA
      241 TGGGCTCGCG ATAATGTCGG GCAATCAGGT GCGACAATCT ATCGATTGTA TGGGAAGCCC
      301 GATGCGCCAG AGTTGTTTCT GAAACATGGC AAAGGTAGCG TTGCCAATGA TGTTACAGAT
      361 GAGATGGTCA GACTAAACTG GCTGACGGAA TTTATGCCTC TTCCGACCAT CAAGCATTTT
      421 ATCCGTACTC CTGATGATGC ATGGTTACTC ACCACTGCGA TCCCCGGCAA AACAGCATTC
      481 CAGGTATTAG AAGAATATCC TGATTCAGGT GAAAATATTG TTGATGCGCT GGCAGTGTTC
      541 CTGCGCCGGT TGCATTCGAT TCCTGTTTGT AATTGTCCTT TTAACAGTGA TCGCGTATTT
      601 CGTCTCGCTC AGGCGCAATC ACGAATGAAT AACGGTTTGG TTGATGCGAG TGATTTTGAT
      661 GACGAGCGTA ATGGCTGGCC TGTTGAACAA GTCTGGAAAG AAATGCATAA ACTTTTGCCA
      721 TTCTCACCGG ATTCAGTCGT CACTCATGGT GATTTCTCAC TTGATAACCT TATTTTTGAC
      781 GAGGGGAAAT TAATAGGTTG TATTGATGTT GGACGAGTCG GAATCGCAGA CCGATACCAG
      841 GATCTTGCCA TCCTATGGAA CTGCCTCGGT GAGTTTTCTC CTTCATTACA GAAACGGCTT
      901 TTTCAAAAAT ATGGTATTGA TAATCCTGAT ATGAATAAAT TGCAGTTTCA TTTGATGCTC
      961 GATGAGTTTT TCTAAGAATT AATTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA
     1021 TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGAAA TTGTAAACGT
     1081 TAATATTTTG TTAAAATTCG CGTTAAATTT TTGTTAAATC AGCTCATTTT TTAACCAATA
     1141 GGCCGAAATC GGCAAAATCC CTTATAAATC AAAAGAATAG ACCGAGATAG GGTTGAGTGT
     1201 TGTTCCAGTT TGGAACAAGA GTCCACTATT AAAGAACGTG GACTCCAACG TCAAAGGGCG
     1261 AAAAACCGTC TATCAGGGCG ATGGCCCACT ACGTGAACCA TCACCCTAAT CAAGTTTTTT
     1321 GGGGTCGAGG TGCCGTAAAG CACTAAATCG GAACCCTAAA GGGAGCCCCC GATTTAGAGC
     1381 TTGACGGGGA AAGCCGGCGA ACGTGGCGAG AAAGGAAGGG AAGAAAGCGA AAGGAGCGGG
     1441 CGCTAGGGCG CTGGCAAGTG TAGCGGTCAC GCTGCGCGTA ACCACCACAC CCGCCGCGCT
     1501 TAATGCGCCG CTACAGGGCG CGTCCCATTC GCCAATCCGG ATATAGTTCC TCCTTTCAGC
     1561 AAAAAACCCC TCAAGACCCG TTTAGAGGCC CCAAGGGGTT ATGCTAGTTA TTGCTCAGCG
     1621 GTGGCAGCAG CCAACTCAGC TTCCTTTCGG GCTTTGTTTA GCAGCCTAGG TTAATTAAGC
     1681 CTCGAGAGCA GCAGAAGTAG AGCTGTCCAT GTGCTGGCGT TCGAATTTAG CAGCAGCGGT
     1741 TTCTTTACTA CCGCGTGGCA CCAGAGCGAG CTCTGCGGCC GCAAGCTTGT CGACGGACGT
     1801 CGGGCGCGCC AAGGCCTGTA CAGAATTCGG ATCCttaATG GTGATGGTGG TGATGGGGTC
     1861 CCTGAAAGAG GACTTCAAGA GCCGCcagat tatttaaccg acgacgtagg tcgtccaatt
     1921 cctggtcaat caccgcatca tcttgactgg cattattagc tggtactgag gaatcaacag
     1981 gagccgcttc gagttggggc gtagcggcag aggttcccgg taatgctcca ccggccatgg
     2041 aagctttcag ggcggccaac tcgtcttcta ccccactgct ggcttccaac tgggcaaact
     2101 ggttctcgat gccaaagccg gctaactccc ctgccgcttg ggaagtggct tccatatcca
     2161 gtaccttgtt ctccatccgt tcaaaagcac tagtagcact gctagtacct aagcccccga
     2221 gggtttgctg cagttcagca ttagccttgg ccgctttggc cctggcctgc aacatatttt
     2281 tcttggtttt agcttcggaa atttttgctt ctagagccgc gaggttgcga cggagattct
     2341 ccgacatggt cctctgttgg gctagctggg tttggtacgc cgccgccgta tctgtcagac
     2401 tttttttgcg ggccagggct tccctagcca aattttcttc cccattggtg agggctaatt
     2461 ttgcccggtc ttcccatttc ttggcttcct gggtgtcttg gttaagacgt tgctctgttc
     2521 gtttttcttc ggcgatcgtg cgggcaacgg cctggcggag ttgcaccagg tcttcctgca
     2581 tatcgatcac ggcctgttcc agaacttttt ctggatccat ATGTATATCT CCTTCTTAAA
     2641 GTTAAACAAA ATTATTTCTA GAGGGGAATT GTTATCCGCT CACAATTCCC CTATAGTGAG
     2701 TCGTATTAAT TTCGCGGGAT CGAGATCGAT CTCGATCCTC TACGCCGGAC GCATCGTGGC
     2761 CGGCATCACC GGCGCCACAG GTGCGGTTGC TGGCGCCTAT ATCGCCGACA TCACCGATGG
     2821 GGAAGATCGG GCTCGCCACT TCGGGCTCAT GAGCGCTTGT TTCGGCGTGG GTATGGTGGC
     2881 AGGCCCCGTG GCCGGGGGAC TGTTGGGCGC CATCTCCTTG CATGCACCAT TCCTTGCGGC
     2941 GGCGGTGCTC AACGGCCTCA ACCTACTACT GGGCTGCTTC CTAATGCAGG AGTCGCATAA
     3001 GGGAGAGCGT CGAGATCCCG GACACCATCG AATGGCGCAA AACCTTTCGC GGTATGGCAT
     3061 GATAGCGCCC GGAAGAGAGT CAATTCAGGG TGGTGAATGT GAAACCAGTA ACGTTATACG
     3121 ATGTCGCAGA GTATGCCGGT GTCTCTTATC AGACCGTTTC CCGCGTGGTG AACCAGGCCA
     3181 GCCACGTTTC TGCGAAAACG CGGGAAAAAG TGGAAGCGGC GATGGCGGAG CTGAATTACA
     3241 TTCCCAACCG CGTGGCACAA CAACTGGCGG GCAAACAGTC GTTGCTGATT GGCGTTGCCA
     3301 CCTCCAGTCT GGCCCTGCAC GCGCCGTCGC AAATTGTCGC GGCGATTAAA TCTCGCGCCG
     3361 ATCAACTGGG TGCCAGCGTG GTGGTGTCGA TGGTAGAACG AAGCGGCGTC GAAGCCTGTA
     3421 AAGCGGCGGT GCACAATCTT CTCGCGCAAC GCGTCAGTGG GCTGATCATT AACTATCCGC
     3481 TGGATGACCA GGATGCCATT GCTGTGGAAG CTGCCTGCAC TAATGTTCCG GCGTTATTTC
     3541 TTGATGTCTC TGACCAGACA CCCATCAACA GTATTATTTT CTCCCATGAA GACGGTACGC
     3601 GACTGGGCGT GGAGCATCTG GTCGCATTGG GTCACCAGCA AATCGCGCTG TTAGCGGGCC
     3661 CATTAAGTTC TGTCTCGGCG CGTCTGCGTC TGGCTGGCTG GCATAAATAT CTCACTCGCA
     3721 ATCAAATTCA GCCGATAGCG GAACGGGAAG GCGACTGGAG TGCCATGTCC GGTTTTCAAC
     3781 AAACCATGCA AATGCTGAAT GAGGGCATCG TTCCCACTGC GATGCTGGTT GCCAACGATC
     3841 AGATGGCGCT GGGCGCAATG CGCGCCATTA CCGAGTCCGG GCTGCGCGTT GGTGCGGACA
     3901 TCTCGGTAGT GGGATACGAC GATACCGAAG ACAGCTCATG TTATATCCCG CCGTTAACCA
     3961 CCATCAAACA GGATTTTCGC CTGCTGGGGC AAACCAGCGT GGACCGCTTG CTGCAACTCT
     4021 CTCAGGGCCA GGCGGTGAAG GGCAATCAGC TGTTGCCCGT CTCACTGGTG AAAAGAAAAA
     4081 CCACCCTGGC GCCCAATACG CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC
     4141 AGCTGGCACG ACAGGTTTCC CGACTGGAAA GCGGGCAGTG AGCGCAACGC AATTAATGTA
     4201 AGTTAGCTCA CTCATTAGGC ACCGGGATCT CGACCGATGC CCTTGAGAGC CTTCAACCCA
     4261 GTCAGCTCCT TCCGGTGGGC GCGGGGCATG ACTAGCATGA TCGTGCTCCT GTCGTTGAGG
     4321 ACCCGGCTAG GCTGGCGGGG TTGCCTTACT GGTTAGCAGA ATGAATCACC GATACGCGAG
     4381 CGAACGTGAA GCGACTGCTG CTGCAAAACG TCTGCGACCT GAGCAACAAC ATGAATGGTC
     4441 TTCGGTTTCC GTGTTTCGTA AAGTCTGGAA ACGCGGAAGT CAGCGCCCTG CACCATTATG
     4501 TTCCGGATCT GCATCGCAGG ATGCTGCTGG CTACCCTGTG GAACACCTAC ATCTGTATTA
     4561 ACGAAGCGCT GGCATTGACC CTGAGTGATT TTTCTCTGGT CCCGCCGCAT CCATACCGCC
     4621 AGTTGTTTAC CCTCACAACG TTCCAGTAAC CGGGCATGTT CATCATCAGT AACCCGTATC
     4681 GTGAGCATCC TCTCTCGTTT CATCGGTATC ATTACCCCCA TGAACAGAAA TCCCCCTTAC
     4741 ACGGAGGCAT CAGTGACCAA ACAGGAAAAA ACCGCCCTTA ACATGGCCCG CTTTATCAGA
     4801 AGCCAGACAT TAACGCTTCT GGAGAAACTC AACGAGCTGG ACGCGGATGA ACAGGCAGAC
     4861 ATCTGTGAAT CGCTTCACGA CCACGCTGAT GAGCTTTACC GCAGCTGCCT CGCGCGTTTC
     4921 GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG AGACGGTCAC AGCTTGTCTG
     4981 TAAGCGGATG CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT
     5041 CGGGGCGCAG CCATGACCCA GTCACGTAGC GATAGCGGAG TGTATACTGG CTTAACTATG
     5101 CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATATATG CGGTGTGAAA TACCGCACAG
     5161 ATGCGTAAGG AGAAAATACC GCATCAGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT
     5221 GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT
     5281 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC
     5341 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA
     5401 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA
     5461 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC
     5521 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG
     5581 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC
     5641 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG
     5701 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT
     5761 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT
     5821 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG
     5881 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTT
//