LOCUS       pET50delCT_Vipp1_dL10Al 5984 bp ds-DNA     circular     10-JUL-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             160..975
                     /label="KanR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     rep_origin      complement(1068..1523)
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     terminator      1560..1607
                     /label="T7 terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(1702..1746)
                     /label="S-Tag"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             complement(1747..1764)
                     /label="thrombin site"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1768..1834
                     /label="MCS"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             complement(1827..2680)
                     /label="Translation 2680-1827"
                     /translation="MGLFDRLGRVVRANLNDLVSKAEDPEKVLEQAVIDMQEDLVQLRQAVARTIAEEKRTEQRLNQDTQEAKKWEDRAKLALTNGEENLAREALARKKSLTDTAAAYQTQLAQQRTMSENLRRNLAALEAKISEAKTKKNMLQARAKAAKANAELQQTLAAAAAAAAAAAFERMENKVLDMEATSQAAGELAGFGIENQFAQLEASSGVEDELAALKASMAGGALPGTSAATPQLEAAPVDSSVPANNASQDDAVIDQELDDLRRRLNAALEVLFQGPHHHHHH*GS"
     misc_feature    complement(1856..1885)
                     /label="3C site"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             complement(1859..2680)
                     /label="Translation 2680-1859"
                     /translation="MGLFDRLGRVVRANLNDLVSKAEDPEKVLEQAVIDMQEDLVQLRQAVARTIAEEKRTEQRLNQDTQEAKKWEDRAKLALTNGEENLAREALARKKSLTDTAAAYQTQLAQQRTMSENLRRNLAALEAKISEAKTKKNMLQARAKAAKANAELQQTLAAAAAAAAAAAFERMENKVLDMEATSQAAGELAGFGIENQFAQLEASSGVEDELAALKASMAGGALPGTSAATPQLEAAPVDSSVPANNASQDDAVIDQELDDLRRRLNAALEVLFQG"
     primer          complement(1866..1885)
                     /label="pET50delCT SynPspA REV"
                     /note="sequence: gtcgtcggttaaatGCGGCTCTTGAAGTCCTCTT"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          1880..1899
                     /label="pRSET6a IM30 wt FWD"
                     /note="sequence: AAGAGGACTTCAAGcagattatttaaccgacgac"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    complement(1886..2680)
                     /label="Vipp1"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          complement(2167..2197)
                     /label="q5_Vipp1_dL10ALA_RV"
                     /note="sequence: gcggcggctgcagcggcttttgaacggatgg"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          2198..2227
                     /label="q5_Vipp1_dL10ALA_FW"
                     /note="sequence: tgcagctgctgccgcgagggtttgctgcag"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(2661..2680)
                     /label="pRSET6a IM30 wt REV"
                     /note="sequence: TAAGAAGGAGATATACATatgggattatttgaccgttt"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          2681..2711
                     /label="pET50delCT SynPspA FWD"
                     /note="sequence: acggtcaaataatcccatATGTATATCTCCTTCTTAAAGTTAAACAAAA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     RBS             2689..2694
                     /label="RBS"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     protein_bind    2725..2749
                     /label="lac operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     promoter        complement(2750..2768)
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        3081..3158
                     /label="lacI promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             3159..4241
                     /label="lacI"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     CDS             4813..5004
                     /label="rop"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     rep_origin      complement(5434..38)
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA
       61 CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAACAATA
      121 AAACTGTCTG CTTACATAAA CAGTAATACA AGGGGTGTTA TGAGCCATAT TCAACGGGAA
      181 ACGTCTTGCT CTAGGCCGCG ATTAAATTCC AACATGGATG CTGATTTATA TGGGTATAAA
      241 TGGGCTCGCG ATAATGTCGG GCAATCAGGT GCGACAATCT ATCGATTGTA TGGGAAGCCC
      301 GATGCGCCAG AGTTGTTTCT GAAACATGGC AAAGGTAGCG TTGCCAATGA TGTTACAGAT
      361 GAGATGGTCA GACTAAACTG GCTGACGGAA TTTATGCCTC TTCCGACCAT CAAGCATTTT
      421 ATCCGTACTC CTGATGATGC ATGGTTACTC ACCACTGCGA TCCCCGGCAA AACAGCATTC
      481 CAGGTATTAG AAGAATATCC TGATTCAGGT GAAAATATTG TTGATGCGCT GGCAGTGTTC
      541 CTGCGCCGGT TGCATTCGAT TCCTGTTTGT AATTGTCCTT TTAACAGTGA TCGCGTATTT
      601 CGTCTCGCTC AGGCGCAATC ACGAATGAAT AACGGTTTGG TTGATGCGAG TGATTTTGAT
      661 GACGAGCGTA ATGGCTGGCC TGTTGAACAA GTCTGGAAAG AAATGCATAA ACTTTTGCCA
      721 TTCTCACCGG ATTCAGTCGT CACTCATGGT GATTTCTCAC TTGATAACCT TATTTTTGAC
      781 GAGGGGAAAT TAATAGGTTG TATTGATGTT GGACGAGTCG GAATCGCAGA CCGATACCAG
      841 GATCTTGCCA TCCTATGGAA CTGCCTCGGT GAGTTTTCTC CTTCATTACA GAAACGGCTT
      901 TTTCAAAAAT ATGGTATTGA TAATCCTGAT ATGAATAAAT TGCAGTTTCA TTTGATGCTC
      961 GATGAGTTTT TCTAAGAATT AATTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA
     1021 TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGAAA TTGTAAACGT
     1081 TAATATTTTG TTAAAATTCG CGTTAAATTT TTGTTAAATC AGCTCATTTT TTAACCAATA
     1141 GGCCGAAATC GGCAAAATCC CTTATAAATC AAAAGAATAG ACCGAGATAG GGTTGAGTGT
     1201 TGTTCCAGTT TGGAACAAGA GTCCACTATT AAAGAACGTG GACTCCAACG TCAAAGGGCG
     1261 AAAAACCGTC TATCAGGGCG ATGGCCCACT ACGTGAACCA TCACCCTAAT CAAGTTTTTT
     1321 GGGGTCGAGG TGCCGTAAAG CACTAAATCG GAACCCTAAA GGGAGCCCCC GATTTAGAGC
     1381 TTGACGGGGA AAGCCGGCGA ACGTGGCGAG AAAGGAAGGG AAGAAAGCGA AAGGAGCGGG
     1441 CGCTAGGGCG CTGGCAAGTG TAGCGGTCAC GCTGCGCGTA ACCACCACAC CCGCCGCGCT
     1501 TAATGCGCCG CTACAGGGCG CGTCCCATTC GCCAATCCGG ATATAGTTCC TCCTTTCAGC
     1561 AAAAAACCCC TCAAGACCCG TTTAGAGGCC CCAAGGGGTT ATGCTAGTTA TTGCTCAGCG
     1621 GTGGCAGCAG CCAACTCAGC TTCCTTTCGG GCTTTGTTTA GCAGCCTAGG TTAATTAAGC
     1681 CTCGAGAGCA GCAGAAGTAG AGCTGTCCAT GTGCTGGCGT TCGAATTTAG CAGCAGCGGT
     1741 TTCTTTACTA CCGCGTGGCA CCAGAGCGAG CTCTGCGGCC GCAAGCTTGT CGACGGACGT
     1801 CGGGCGCGCC AAGGCCTGTA CAGAATTCGG ATCCttaATG GTGATGGTGG TGATGGGGTC
     1861 CCTGAAAGAG GACTTCAAGA GCCGCattta accgacgacg taggtcgtcc aattcctggt
     1921 caatcaccgc atcatcttga ctggcattat tagctggtac tgaggaatca acaggagccg
     1981 cttcgagttg gggcgtagcg gcagaggttc ccggtaatgc tccaccggcc atggaagctt
     2041 tcagggcggc caactcgtct tctaccccac tgctggcttc caactgggca aactggttct
     2101 cgatgccaaa gccggctaac tcccctgccg cttgggaagt ggcttccata tccagtacct
     2161 tgttctccat ccgttcaaaa gccgctgcag ccgccgctgc agctgctgcc gcgagggttt
     2221 gctgcagttc agcattagcc ttggccgctt tggccctggc ctgcaacata tttttcttgg
     2281 ttttagcttc ggaaattttt gcttctagag ccgcgaggtt gcgacggaga ttctccgaca
     2341 tggtcctctg ttgggctagc tgggtttggt acgccgccgc cgtatctgtc agactttttt
     2401 tgcgggccag ggcttcccta gccaaatttt cttccccatt ggtgagggct aattttgccc
     2461 ggtcttccca tttcttggct tcctgggtgt cttggttaag acgttgctct gttcgttttt
     2521 cttcggcgat cgtgcgggca acggcctggc ggagttgcac caggtcttcc tgcatatcga
     2581 tcacggcctg ttccagaact ttttctggat cttcagcttt actgacgagg tcgttgaggt
     2641 tagcccggac gacgcggcct aaacggtcaa ataatcccat ATGTATATCT CCTTCTTAAA
     2701 GTTAAACAAA ATTATTTCTA GAGGGGAATT GTTATCCGCT CACAATTCCC CTATAGTGAG
     2761 TCGTATTAAT TTCGCGGGAT CGAGATCGAT CTCGATCCTC TACGCCGGAC GCATCGTGGC
     2821 CGGCATCACC GGCGCCACAG GTGCGGTTGC TGGCGCCTAT ATCGCCGACA TCACCGATGG
     2881 GGAAGATCGG GCTCGCCACT TCGGGCTCAT GAGCGCTTGT TTCGGCGTGG GTATGGTGGC
     2941 AGGCCCCGTG GCCGGGGGAC TGTTGGGCGC CATCTCCTTG CATGCACCAT TCCTTGCGGC
     3001 GGCGGTGCTC AACGGCCTCA ACCTACTACT GGGCTGCTTC CTAATGCAGG AGTCGCATAA
     3061 GGGAGAGCGT CGAGATCCCG GACACCATCG AATGGCGCAA AACCTTTCGC GGTATGGCAT
     3121 GATAGCGCCC GGAAGAGAGT CAATTCAGGG TGGTGAATGT GAAACCAGTA ACGTTATACG
     3181 ATGTCGCAGA GTATGCCGGT GTCTCTTATC AGACCGTTTC CCGCGTGGTG AACCAGGCCA
     3241 GCCACGTTTC TGCGAAAACG CGGGAAAAAG TGGAAGCGGC GATGGCGGAG CTGAATTACA
     3301 TTCCCAACCG CGTGGCACAA CAACTGGCGG GCAAACAGTC GTTGCTGATT GGCGTTGCCA
     3361 CCTCCAGTCT GGCCCTGCAC GCGCCGTCGC AAATTGTCGC GGCGATTAAA TCTCGCGCCG
     3421 ATCAACTGGG TGCCAGCGTG GTGGTGTCGA TGGTAGAACG AAGCGGCGTC GAAGCCTGTA
     3481 AAGCGGCGGT GCACAATCTT CTCGCGCAAC GCGTCAGTGG GCTGATCATT AACTATCCGC
     3541 TGGATGACCA GGATGCCATT GCTGTGGAAG CTGCCTGCAC TAATGTTCCG GCGTTATTTC
     3601 TTGATGTCTC TGACCAGACA CCCATCAACA GTATTATTTT CTCCCATGAA GACGGTACGC
     3661 GACTGGGCGT GGAGCATCTG GTCGCATTGG GTCACCAGCA AATCGCGCTG TTAGCGGGCC
     3721 CATTAAGTTC TGTCTCGGCG CGTCTGCGTC TGGCTGGCTG GCATAAATAT CTCACTCGCA
     3781 ATCAAATTCA GCCGATAGCG GAACGGGAAG GCGACTGGAG TGCCATGTCC GGTTTTCAAC
     3841 AAACCATGCA AATGCTGAAT GAGGGCATCG TTCCCACTGC GATGCTGGTT GCCAACGATC
     3901 AGATGGCGCT GGGCGCAATG CGCGCCATTA CCGAGTCCGG GCTGCGCGTT GGTGCGGACA
     3961 TCTCGGTAGT GGGATACGAC GATACCGAAG ACAGCTCATG TTATATCCCG CCGTTAACCA
     4021 CCATCAAACA GGATTTTCGC CTGCTGGGGC AAACCAGCGT GGACCGCTTG CTGCAACTCT
     4081 CTCAGGGCCA GGCGGTGAAG GGCAATCAGC TGTTGCCCGT CTCACTGGTG AAAAGAAAAA
     4141 CCACCCTGGC GCCCAATACG CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC
     4201 AGCTGGCACG ACAGGTTTCC CGACTGGAAA GCGGGCAGTG AGCGCAACGC AATTAATGTA
     4261 AGTTAGCTCA CTCATTAGGC ACCGGGATCT CGACCGATGC CCTTGAGAGC CTTCAACCCA
     4321 GTCAGCTCCT TCCGGTGGGC GCGGGGCATG ACTAGCATGA TCGTGCTCCT GTCGTTGAGG
     4381 ACCCGGCTAG GCTGGCGGGG TTGCCTTACT GGTTAGCAGA ATGAATCACC GATACGCGAG
     4441 CGAACGTGAA GCGACTGCTG CTGCAAAACG TCTGCGACCT GAGCAACAAC ATGAATGGTC
     4501 TTCGGTTTCC GTGTTTCGTA AAGTCTGGAA ACGCGGAAGT CAGCGCCCTG CACCATTATG
     4561 TTCCGGATCT GCATCGCAGG ATGCTGCTGG CTACCCTGTG GAACACCTAC ATCTGTATTA
     4621 ACGAAGCGCT GGCATTGACC CTGAGTGATT TTTCTCTGGT CCCGCCGCAT CCATACCGCC
     4681 AGTTGTTTAC CCTCACAACG TTCCAGTAAC CGGGCATGTT CATCATCAGT AACCCGTATC
     4741 GTGAGCATCC TCTCTCGTTT CATCGGTATC ATTACCCCCA TGAACAGAAA TCCCCCTTAC
     4801 ACGGAGGCAT CAGTGACCAA ACAGGAAAAA ACCGCCCTTA ACATGGCCCG CTTTATCAGA
     4861 AGCCAGACAT TAACGCTTCT GGAGAAACTC AACGAGCTGG ACGCGGATGA ACAGGCAGAC
     4921 ATCTGTGAAT CGCTTCACGA CCACGCTGAT GAGCTTTACC GCAGCTGCCT CGCGCGTTTC
     4981 GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG AGACGGTCAC AGCTTGTCTG
     5041 TAAGCGGATG CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT
     5101 CGGGGCGCAG CCATGACCCA GTCACGTAGC GATAGCGGAG TGTATACTGG CTTAACTATG
     5161 CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATATATG CGGTGTGAAA TACCGCACAG
     5221 ATGCGTAAGG AGAAAATACC GCATCAGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT
     5281 GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT
     5341 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC
     5401 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA
     5461 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA
     5521 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC
     5581 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG
     5641 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC
     5701 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG
     5761 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT
     5821 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT
     5881 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG
     5941 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTT
//