LOCUS       pET50b_del_(G)_SynPspA  5879 bp ds-DNA     circular     03-JAN-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     primer          1..20
                     /label="pET50b SynPspA FWD"
                     /note="sequence: CCTGTACAGAATTCGGATCCttacgagcttggcggcggta"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    complement(1..672)
                     /label="synPspA"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             complement(1..672)
                     /label="Translation 672-1"
                     /translation="MELFNRVGRVLKSQLTHWQQQQEAPEDLLERLLGEMELELIELRRALAQTIATFKSTERQRDAQQLIAQRWYEKAQAALDRGNEQLAREALGQRQSYQSHTEALGKSLGEQRALVEQVRGQLQKLERKYLELKSQKNLYLARLKSAIAAQKIEEIAGNLDNASASSLFERIETKILELEAERELLNPPPSPLDKKFEQWEEQQAVEATLAAMKARRSLPPPSS*"
     CDS             complement(1..741)
                     /label="Translation 741-1"
                     /translation="MGSSHHHHHHSSSAALEVLFQGPMELFNRVGRVLKSQLTHWQQQQEAPEDLLERLLGEMELELIELRRALAQTIATFKSTERQRDAQQLIAQRWYEKAQAALDRGNEQLAREALGQRQSYQSHTEALGKSLGEQRALVEQVRGQLQKLERKYLELKSQKNLYLARLKSAIAAQKIEEIAGNLDNASASSLFERIETKILELEAERELLNPPPSPLDKKFEQWEEQQAVEATLAAMKARRSLPPPSS*"
     misc_feature    604..672
                     /label="H0"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          complement(640..672)
                     /label="pET50b SynPspA REV"
                     /note="sequence: AAGTCCTCTTTCAGGGACCCatggaactatttaatagggttggaagagtattg"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          673..692
                     /label="pET-50del_FWD"
                     /note="sequence: accctattaaatagttccatGGGTCCCTGAAAGAGGACTT"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             complement(673..696)
                     /label="HRV 3C site"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             complement(712..729)
                     /label="6xHis"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             complement(739..741)
                     /label="ATG"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     RBS             750..755
                     /label="RBS"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     protein_bind    786..810
                     /label="lac operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     promoter        complement(811..829)
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        1142..1219
                     /label="lacI promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             1220..2302
                     /label="lacI"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     CDS             2874..3065
                     /label="rop"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     rep_origin      complement(3495..4083)
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             4205..5020
                     /label="KanR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     rep_origin      complement(5113..5568)
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     terminator      5605..5652
                     /label="T7 terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(5747..5791)
                     /label="S-Tag"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             complement(5792..5809)
                     /label="thrombin site"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    5813..5879
                     /label="MCS"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          complement(5860..5879)
                     /label="pET-50del_REV"
                     /note="sequence: taccgccgccaagctcgtaaGGATCCGAATTCTGTACAGG"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
ORIGIN
        1 ttacgagctt ggcggcggta agctacggcg cgctttcatg gcagccaggg ttgcttccac
       61 agcttgttgc tcttcccact gctcgaattt tttgtccagc ggggacggag gcggattcaa
      121 cagttcacgt tcggcctcta actccaggat tttggtttca atccgttcga acagagagct
      181 tgcgctcgcg ttgtccaggt tacccgcgat ctcctcgatc ttctgcgcag caattgctga
      241 cttcaggcgg gccagataaa ggttcttctg gctcttaagc tccaggtatt tacgctccag
      301 cttttgcagc tggcctctaa cctgctcgac caatgcacgt tgttcaccca gagatttgcc
      361 gagcgcttcg gtgtgggact gatagctctg acgctgaccc agcgcttcac gagctagttg
      421 ctcattacca cggtccaacg ccgcctgcgc cttttcgtac caacgttggg caatcagctg
      481 ttgcgcatca cgttggcgct cagtgctttt aaacgtcgca atggtctgcg ccagtgcgcg
      541 acgcagctcg atcagctcca attccatttc gcccagcagg cgctccaaca gatcttccgg
      601 agcttcttgc tgctgctgcc aatgggtcaa ttgggacttc aatactcttc caaccctatt
      661 aaatagttcc atGGGTCCCT GAAAGAGGAC TTCAAGAGCC GCGGAACTAG AATGGTGATG
      721 GTGGTGATGG CTGCTGCCCA TATGTATATC TCCTTCTTAA AGTTAAACAA AATTATTTCT
      781 AGAGGGGAAT TGTTATCCGC TCACAATTCC CCTATAGTGA GTCGTATTAA TTTCGCGGGA
      841 TCGAGATCGA TCTCGATCCT CTACGCCGGA CGCATCGTGG CCGGCATCAC CGGCGCCACA
      901 GGTGCGGTTG CTGGCGCCTA TATCGCCGAC ATCACCGATG GGGAAGATCG GGCTCGCCAC
      961 TTCGGGCTCA TGAGCGCTTG TTTCGGCGTG GGTATGGTGG CAGGCCCCGT GGCCGGGGGA
     1021 CTGTTGGGCG CCATCTCCTT GCATGCACCA TTCCTTGCGG CGGCGGTGCT CAACGGCCTC
     1081 AACCTACTAC TGGGCTGCTT CCTAATGCAG GAGTCGCATA AGGGAGAGCG TCGAGATCCC
     1141 GGACACCATC GAATGGCGCA AAACCTTTCG CGGTATGGCA TGATAGCGCC CGGAAGAGAG
     1201 TCAATTCAGG GTGGTGAATG TGAAACCAGT AACGTTATAC GATGTCGCAG AGTATGCCGG
     1261 TGTCTCTTAT CAGACCGTTT CCCGCGTGGT GAACCAGGCC AGCCACGTTT CTGCGAAAAC
     1321 GCGGGAAAAA GTGGAAGCGG CGATGGCGGA GCTGAATTAC ATTCCCAACC GCGTGGCACA
     1381 ACAACTGGCG GGCAAACAGT CGTTGCTGAT TGGCGTTGCC ACCTCCAGTC TGGCCCTGCA
     1441 CGCGCCGTCG CAAATTGTCG CGGCGATTAA ATCTCGCGCC GATCAACTGG GTGCCAGCGT
     1501 GGTGGTGTCG ATGGTAGAAC GAAGCGGCGT CGAAGCCTGT AAAGCGGCGG TGCACAATCT
     1561 TCTCGCGCAA CGCGTCAGTG GGCTGATCAT TAACTATCCG CTGGATGACC AGGATGCCAT
     1621 TGCTGTGGAA GCTGCCTGCA CTAATGTTCC GGCGTTATTT CTTGATGTCT CTGACCAGAC
     1681 ACCCATCAAC AGTATTATTT TCTCCCATGA AGACGGTACG CGACTGGGCG TGGAGCATCT
     1741 GGTCGCATTG GGTCACCAGC AAATCGCGCT GTTAGCGGGC CCATTAAGTT CTGTCTCGGC
     1801 GCGTCTGCGT CTGGCTGGCT GGCATAAATA TCTCACTCGC AATCAAATTC AGCCGATAGC
     1861 GGAACGGGAA GGCGACTGGA GTGCCATGTC CGGTTTTCAA CAAACCATGC AAATGCTGAA
     1921 TGAGGGCATC GTTCCCACTG CGATGCTGGT TGCCAACGAT CAGATGGCGC TGGGCGCAAT
     1981 GCGCGCCATT ACCGAGTCCG GGCTGCGCGT TGGTGCGGAC ATCTCGGTAG TGGGATACGA
     2041 CGATACCGAA GACAGCTCAT GTTATATCCC GCCGTTAACC ACCATCAAAC AGGATTTTCG
     2101 CCTGCTGGGG CAAACCAGCG TGGACCGCTT GCTGCAACTC TCTCAGGGCC AGGCGGTGAA
     2161 GGGCAATCAG CTGTTGCCCG TCTCACTGGT GAAAAGAAAA ACCACCCTGG CGCCCAATAC
     2221 GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC
     2281 CCGACTGGAA AGCGGGCAGT GAGCGCAACG CAATTAATGT AAGTTAGCTC ACTCATTAGG
     2341 CACCGGGATC TCGACCGATG CCCTTGAGAG CCTTCAACCC AGTCAGCTCC TTCCGGTGGG
     2401 CGCGGGGCAT GACTAGCATG ATCGTGCTCC TGTCGTTGAG GACCCGGCTA GGCTGGCGGG
     2461 GTTGCCTTAC TGGTTAGCAG AATGAATCAC CGATACGCGA GCGAACGTGA AGCGACTGCT
     2521 GCTGCAAAAC GTCTGCGACC TGAGCAACAA CATGAATGGT CTTCGGTTTC CGTGTTTCGT
     2581 AAAGTCTGGA AACGCGGAAG TCAGCGCCCT GCACCATTAT GTTCCGGATC TGCATCGCAG
     2641 GATGCTGCTG GCTACCCTGT GGAACACCTA CATCTGTATT AACGAAGCGC TGGCATTGAC
     2701 CCTGAGTGAT TTTTCTCTGG TCCCGCCGCA TCCATACCGC CAGTTGTTTA CCCTCACAAC
     2761 GTTCCAGTAA CCGGGCATGT TCATCATCAG TAACCCGTAT CGTGAGCATC CTCTCTCGTT
     2821 TCATCGGTAT CATTACCCCC ATGAACAGAA ATCCCCCTTA CACGGAGGCA TCAGTGACCA
     2881 AACAGGAAAA AACCGCCCTT AACATGGCCC GCTTTATCAG AAGCCAGACA TTAACGCTTC
     2941 TGGAGAAACT CAACGAGCTG GACGCGGATG AACAGGCAGA CATCTGTGAA TCGCTTCACG
     3001 ACCACGCTGA TGAGCTTTAC CGCAGCTGCC TCGCGCGTTT CGGTGATGAC GGTGAAAACC
     3061 TCTGACACAT GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA
     3121 GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCGCA GCCATGACCC
     3181 AGTCACGTAG CGATAGCGGA GTGTATACTG GCTTAACTAT GCGGCATCAG AGCAGATTGT
     3241 ACTGAGAGTG CACCATATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC
     3301 CGCATCAGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG
     3361 CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT
     3421 AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC
     3481 GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC
     3541 TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA
     3601 AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT
     3661 CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG
     3721 TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC
     3781 GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG
     3841 GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC
     3901 TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG
     3961 CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC
     4021 GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT
     4081 CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT
     4141 TAAGGGATTT TGGTCATGAA CAATAAAACT GTCTGCTTAC ATAAACAGTA ATACAAGGGG
     4201 TGTTATGAGC CATATTCAAC GGGAAACGTC TTGCTCTAGG CCGCGATTAA ATTCCAACAT
     4261 GGATGCTGAT TTATATGGGT ATAAATGGGC TCGCGATAAT GTCGGGCAAT CAGGTGCGAC
     4321 AATCTATCGA TTGTATGGGA AGCCCGATGC GCCAGAGTTG TTTCTGAAAC ATGGCAAAGG
     4381 TAGCGTTGCC AATGATGTTA CAGATGAGAT GGTCAGACTA AACTGGCTGA CGGAATTTAT
     4441 GCCTCTTCCG ACCATCAAGC ATTTTATCCG TACTCCTGAT GATGCATGGT TACTCACCAC
     4501 TGCGATCCCC GGCAAAACAG CATTCCAGGT ATTAGAAGAA TATCCTGATT CAGGTGAAAA
     4561 TATTGTTGAT GCGCTGGCAG TGTTCCTGCG CCGGTTGCAT TCGATTCCTG TTTGTAATTG
     4621 TCCTTTTAAC AGTGATCGCG TATTTCGTCT CGCTCAGGCG CAATCACGAA TGAATAACGG
     4681 TTTGGTTGAT GCGAGTGATT TTGATGACGA GCGTAATGGC TGGCCTGTTG AACAAGTCTG
     4741 GAAAGAAATG CATAAACTTT TGCCATTCTC ACCGGATTCA GTCGTCACTC ATGGTGATTT
     4801 CTCACTTGAT AACCTTATTT TTGACGAGGG GAAATTAATA GGTTGTATTG ATGTTGGACG
     4861 AGTCGGAATC GCAGACCGAT ACCAGGATCT TGCCATCCTA TGGAACTGCC TCGGTGAGTT
     4921 TTCTCCTTCA TTACAGAAAC GGCTTTTTCA AAAATATGGT ATTGATAATC CTGATATGAA
     4981 TAAATTGCAG TTTCATTTGA TGCTCGATGA GTTTTTCTAA GAATTAATTC ATGAGCGGAT
     5041 ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA
     5101 AAGTGCCACC TGAAATTGTA AACGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT
     5161 AAATCAGCTC ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT AAATCAAAAG
     5221 AATAGACCGA GATAGGGTTG AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA
     5281 ACGTGGACTC CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG
     5341 AACCATCACC CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA AATCGGAACC
     5401 CTAAAGGGAG CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG GCGAGAAAGG
     5461 AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG GTCACGCTGC
     5521 GCGTAACCAC CACACCCGCC GCGCTTAATG CGCCGCTACA GGGCGCGTCC CATTCGCCAA
     5581 TCCGGATATA GTTCCTCCTT TCAGCAAAAA ACCCCTCAAG ACCCGTTTAG AGGCCCCAAG
     5641 GGGTTATGCT AGTTATTGCT CAGCGGTGGC AGCAGCCAAC TCAGCTTCCT TTCGGGCTTT
     5701 GTTTAGCAGC CTAGGTTAAT TAAGCCTCGA GAGCAGCAGA AGTAGAGCTG TCCATGTGCT
     5761 GGCGTTCGAA TTTAGCAGCA GCGGTTTCTT TACTACCGCG TGGCACCAGA GCGAGCTCTG
     5821 CGGCCGCAAG CTTGTCGACG GACGTCGGGC GCGCCAAGGC CTGTACAGAA TTCGGATCC
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