LOCUS       pET50b_del_(G)_SynPspA_ 5879 bp ds-DNA     circular     14-MAR-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             122..937
                     /label="KanR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     rep_origin      complement(1030..1485)
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     terminator      1522..1569
                     /label="T7 terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(1664..1708)
                     /label="S-Tag"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             complement(1709..1726)
                     /label="thrombin site"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1730..1796
                     /label="MCS"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          complement(1777..1796)
                     /label="pET-50del_REV"
                     /note="sequence: taccgccgccaagctcgtaaGGATCCGAATTCTGTACAGG"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          1797..1816
                     /label="pET50b SynPspA FWD"
                     /note="sequence: CCTGTACAGAATTCGGATCCttacgagcttggcggcggta"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    complement(1797..2468)
                     /label="synPspA"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             complement(1797..2468)
                     /label="Translation 2468-1797"
                     /translation="MELFNRVGRVLKSQLTHWQQQQEAPEDLLERLLGEMELELIELKRALAQTIATFKSTERQRDAQQLIAQRWYEKAQAALDRGNEQLAREALGQRQSYQSHTEALGKSLGEQRALVEQVRGQLQKLQRKYLELKSQKNLYLARLKSAIAAQKIEEIAGNLDNASASSLFERIETKILELQAERELLNPPPSPLDKKFEQWEEQQAVEATLAAMKARRSLPPPSS*"
     primer          complement(2309..2326)
                     /label="Q5_SynPspA(G)_R44K_R"
                     /note="sequence: cgcagaccattgcgacgt"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          2327..2351
                     /label="Q5_SynPspA(G)_R44K_FW"
                     /note="sequence: ccagtgcgcgtttcagctcgatcag"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(2436..2468)
                     /label="pET50b SynPspA REV"
                     /note="sequence: AAGTCCTCTTTCAGGGACCCatggaactatttaatagggttggaagagtattg"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          2469..2488
                     /label="pET-50del_FWD"
                     /note="sequence: accctattaaatagttccatGGGTCCCTGAAAGAGGACTT"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             complement(2469..2492)
                     /label="HRV 3C site"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             complement(2508..2525)
                     /label="6xHis"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             complement(2535..2537)
                     /label="ATG"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     RBS             2546..2551
                     /label="RBS"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     protein_bind    2582..2606
                     /label="lac operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     promoter        complement(2607..2625)
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        2938..3015
                     /label="lacI promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             3016..4098
                     /label="lacI"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     CDS             4670..4861
                     /label="rop"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     rep_origin      complement(5291..5879)
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA
       61 GGGATTTTGG TCATGAACAA TAAAACTGTC TGCTTACATA AACAGTAATA CAAGGGGTGT
      121 TATGAGCCAT ATTCAACGGG AAACGTCTTG CTCTAGGCCG CGATTAAATT CCAACATGGA
      181 TGCTGATTTA TATGGGTATA AATGGGCTCG CGATAATGTC GGGCAATCAG GTGCGACAAT
      241 CTATCGATTG TATGGGAAGC CCGATGCGCC AGAGTTGTTT CTGAAACATG GCAAAGGTAG
      301 CGTTGCCAAT GATGTTACAG ATGAGATGGT CAGACTAAAC TGGCTGACGG AATTTATGCC
      361 TCTTCCGACC ATCAAGCATT TTATCCGTAC TCCTGATGAT GCATGGTTAC TCACCACTGC
      421 GATCCCCGGC AAAACAGCAT TCCAGGTATT AGAAGAATAT CCTGATTCAG GTGAAAATAT
      481 TGTTGATGCG CTGGCAGTGT TCCTGCGCCG GTTGCATTCG ATTCCTGTTT GTAATTGTCC
      541 TTTTAACAGT GATCGCGTAT TTCGTCTCGC TCAGGCGCAA TCACGAATGA ATAACGGTTT
      601 GGTTGATGCG AGTGATTTTG ATGACGAGCG TAATGGCTGG CCTGTTGAAC AAGTCTGGAA
      661 AGAAATGCAT AAACTTTTGC CATTCTCACC GGATTCAGTC GTCACTCATG GTGATTTCTC
      721 ACTTGATAAC CTTATTTTTG ACGAGGGGAA ATTAATAGGT TGTATTGATG TTGGACGAGT
      781 CGGAATCGCA GACCGATACC AGGATCTTGC CATCCTATGG AACTGCCTCG GTGAGTTTTC
      841 TCCTTCATTA CAGAAACGGC TTTTTCAAAA ATATGGTATT GATAATCCTG ATATGAATAA
      901 ATTGCAGTTT CATTTGATGC TCGATGAGTT TTTCTAAGAA TTAATTCATG AGCGGATACA
      961 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG
     1021 TGCCACCTGA AATTGTAAAC GTTAATATTT TGTTAAAATT CGCGTTAAAT TTTTGTTAAA
     1081 TCAGCTCATT TTTTAACCAA TAGGCCGAAA TCGGCAAAAT CCCTTATAAA TCAAAAGAAT
     1141 AGACCGAGAT AGGGTTGAGT GTTGTTCCAG TTTGGAACAA GAGTCCACTA TTAAAGAACG
     1201 TGGACTCCAA CGTCAAAGGG CGAAAAACCG TCTATCAGGG CGATGGCCCA CTACGTGAAC
     1261 CATCACCCTA ATCAAGTTTT TTGGGGTCGA GGTGCCGTAA AGCACTAAAT CGGAACCCTA
     1321 AAGGGAGCCC CCGATTTAGA GCTTGACGGG GAAAGCCGGC GAACGTGGCG AGAAAGGAAG
     1381 GGAAGAAAGC GAAAGGAGCG GGCGCTAGGG CGCTGGCAAG TGTAGCGGTC ACGCTGCGCG
     1441 TAACCACCAC ACCCGCCGCG CTTAATGCGC CGCTACAGGG CGCGTCCCAT TCGCCAATCC
     1501 GGATATAGTT CCTCCTTTCA GCAAAAAACC CCTCAAGACC CGTTTAGAGG CCCCAAGGGG
     1561 TTATGCTAGT TATTGCTCAG CGGTGGCAGC AGCCAACTCA GCTTCCTTTC GGGCTTTGTT
     1621 TAGCAGCCTA GGTTAATTAA GCCTCGAGAG CAGCAGAAGT AGAGCTGTCC ATGTGCTGGC
     1681 GTTCGAATTT AGCAGCAGCG GTTTCTTTAC TACCGCGTGG CACCAGAGCG AGCTCTGCGG
     1741 CCGCAAGCTT GTCGACGGAC GTCGGGCGCG CCAAGGCCTG TACAGAATTC GGATCCttac
     1801 gagcttggcg gcggtaagct acggcgcgct ttcatggcag ccagggttgc ttccacagct
     1861 tgttgctctt cccactgctc gaattttttg tccagcgggg acggaggcgg attcaacagt
     1921 tcacgttcgg cctGtaactc caggattttg gtttcaatcc gttcgaacag agagcttgcg
     1981 ctcgcgttgt ccaggttacc cgcgatctcc tcgatcttct gcgcagcaat tgctgacttc
     2041 aggcgggcca gataaaggtt cttctggctc ttaagctcca ggtatttacg ctgcagcttt
     2101 tgcagctggc ctctaacctg ctcgaccaat gcacgttgtt cacccagaga tttgccgagc
     2161 gcttcggtgt gggactgata gctctgacgc tgacccagcg cttcacgagc tagttgctca
     2221 ttaccacggt ccaacgccgc ctgcgccttt tcgtaccaac gttgggcaat cagctgttgc
     2281 gcatcacgtt ggcgctcagt gcttttaaac gtcgcaatgg tctgcgccag tgcgcgtttc
     2341 agctcgatca gctccaattc catttcgccc agcaggcgct ccaacagatc ttccggagct
     2401 tcttgctgct gctgccaatg ggtcaattgg gacttcaata ctcttccaac cctattaaat
     2461 agttccatGG GTCCCTGAAA GAGGACTTCA AGAGCCGCGG AACTAGAATG GTGATGGTGG
     2521 TGATGGCTGC TGCCCATATG TATATCTCCT TCTTAAAGTT AAACAAAATT ATTTCTAGAG
     2581 GGGAATTGTT ATCCGCTCAC AATTCCCCTA TAGTGAGTCG TATTAATTTC GCGGGATCGA
     2641 GATCGATCTC GATCCTCTAC GCCGGACGCA TCGTGGCCGG CATCACCGGC GCCACAGGTG
     2701 CGGTTGCTGG CGCCTATATC GCCGACATCA CCGATGGGGA AGATCGGGCT CGCCACTTCG
     2761 GGCTCATGAG CGCTTGTTTC GGCGTGGGTA TGGTGGCAGG CCCCGTGGCC GGGGGACTGT
     2821 TGGGCGCCAT CTCCTTGCAT GCACCATTCC TTGCGGCGGC GGTGCTCAAC GGCCTCAACC
     2881 TACTACTGGG CTGCTTCCTA ATGCAGGAGT CGCATAAGGG AGAGCGTCGA GATCCCGGAC
     2941 ACCATCGAAT GGCGCAAAAC CTTTCGCGGT ATGGCATGAT AGCGCCCGGA AGAGAGTCAA
     3001 TTCAGGGTGG TGAATGTGAA ACCAGTAACG TTATACGATG TCGCAGAGTA TGCCGGTGTC
     3061 TCTTATCAGA CCGTTTCCCG CGTGGTGAAC CAGGCCAGCC ACGTTTCTGC GAAAACGCGG
     3121 GAAAAAGTGG AAGCGGCGAT GGCGGAGCTG AATTACATTC CCAACCGCGT GGCACAACAA
     3181 CTGGCGGGCA AACAGTCGTT GCTGATTGGC GTTGCCACCT CCAGTCTGGC CCTGCACGCG
     3241 CCGTCGCAAA TTGTCGCGGC GATTAAATCT CGCGCCGATC AACTGGGTGC CAGCGTGGTG
     3301 GTGTCGATGG TAGAACGAAG CGGCGTCGAA GCCTGTAAAG CGGCGGTGCA CAATCTTCTC
     3361 GCGCAACGCG TCAGTGGGCT GATCATTAAC TATCCGCTGG ATGACCAGGA TGCCATTGCT
     3421 GTGGAAGCTG CCTGCACTAA TGTTCCGGCG TTATTTCTTG ATGTCTCTGA CCAGACACCC
     3481 ATCAACAGTA TTATTTTCTC CCATGAAGAC GGTACGCGAC TGGGCGTGGA GCATCTGGTC
     3541 GCATTGGGTC ACCAGCAAAT CGCGCTGTTA GCGGGCCCAT TAAGTTCTGT CTCGGCGCGT
     3601 CTGCGTCTGG CTGGCTGGCA TAAATATCTC ACTCGCAATC AAATTCAGCC GATAGCGGAA
     3661 CGGGAAGGCG ACTGGAGTGC CATGTCCGGT TTTCAACAAA CCATGCAAAT GCTGAATGAG
     3721 GGCATCGTTC CCACTGCGAT GCTGGTTGCC AACGATCAGA TGGCGCTGGG CGCAATGCGC
     3781 GCCATTACCG AGTCCGGGCT GCGCGTTGGT GCGGACATCT CGGTAGTGGG ATACGACGAT
     3841 ACCGAAGACA GCTCATGTTA TATCCCGCCG TTAACCACCA TCAAACAGGA TTTTCGCCTG
     3901 CTGGGGCAAA CCAGCGTGGA CCGCTTGCTG CAACTCTCTC AGGGCCAGGC GGTGAAGGGC
     3961 AATCAGCTGT TGCCCGTCTC ACTGGTGAAA AGAAAAACCA CCCTGGCGCC CAATACGCAA
     4021 ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA
     4081 CTGGAAAGCG GGCAGTGAGC GCAACGCAAT TAATGTAAGT TAGCTCACTC ATTAGGCACC
     4141 GGGATCTCGA CCGATGCCCT TGAGAGCCTT CAACCCAGTC AGCTCCTTCC GGTGGGCGCG
     4201 GGGCATGACT AGCATGATCG TGCTCCTGTC GTTGAGGACC CGGCTAGGCT GGCGGGGTTG
     4261 CCTTACTGGT TAGCAGAATG AATCACCGAT ACGCGAGCGA ACGTGAAGCG ACTGCTGCTG
     4321 CAAAACGTCT GCGACCTGAG CAACAACATG AATGGTCTTC GGTTTCCGTG TTTCGTAAAG
     4381 TCTGGAAACG CGGAAGTCAG CGCCCTGCAC CATTATGTTC CGGATCTGCA TCGCAGGATG
     4441 CTGCTGGCTA CCCTGTGGAA CACCTACATC TGTATTAACG AAGCGCTGGC ATTGACCCTG
     4501 AGTGATTTTT CTCTGGTCCC GCCGCATCCA TACCGCCAGT TGTTTACCCT CACAACGTTC
     4561 CAGTAACCGG GCATGTTCAT CATCAGTAAC CCGTATCGTG AGCATCCTCT CTCGTTTCAT
     4621 CGGTATCATT ACCCCCATGA ACAGAAATCC CCCTTACACG GAGGCATCAG TGACCAAACA
     4681 GGAAAAAACC GCCCTTAACA TGGCCCGCTT TATCAGAAGC CAGACATTAA CGCTTCTGGA
     4741 GAAACTCAAC GAGCTGGACG CGGATGAACA GGCAGACATC TGTGAATCGC TTCACGACCA
     4801 CGCTGATGAG CTTTACCGCA GCTGCCTCGC GCGTTTCGGT GATGACGGTG AAAACCTCTG
     4861 ACACATGCAG CTCCCGGAGA CGGTCACAGC TTGTCTGTAA GCGGATGCCG GGAGCAGACA
     4921 AGCCCGTCAG GGCGCGTCAG CGGGTGTTGG CGGGTGTCGG GGCGCAGCCA TGACCCAGTC
     4981 ACGTAGCGAT AGCGGAGTGT ATACTGGCTT AACTATGCGG CATCAGAGCA GATTGTACTG
     5041 AGAGTGCACC ATATATGCGG TGTGAAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA
     5101 TCAGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC
     5161 GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG
     5221 CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT
     5281 TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA
     5341 GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT
     5401 CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC
     5461 CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG
     5521 TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT
     5581 TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG
     5641 CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA
     5701 AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT TGGTATCTGC GCTCTGCTGA
     5761 AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG
     5821 GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAA
//