LOCUS       pET50bdel_SynPspA_H1-5  5810 bp ds-DNA     circular     19-JUL-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      complement(363..951)
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             1073..1888
                     /label="KanR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     rep_origin      complement(1981..2436)
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     terminator      2473..2520
                     /label="T7 terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(2615..2659)
                     /label="S-Tag"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             complement(2660..2677)
                     /label="thrombin site"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2681..2747
                     /label="MCS"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          complement(2728..2747)
                     /label="pET-50del_REV"
                     /note="sequence: taccgccgccaagctcgtaaGGATCCGAATTCTGTACAGG"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          2748..2767
                     /label="pET50b SynPspA FWD"
                     /note="sequence: CCTGTACAGAATTCGGATCCttacgagcttggcggcggta"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    complement(2748..3350)
                     /label="synPspA"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             complement(2748..3350)
                     /label="Translation 3350-2748"
                     /translation="APEDLLERLLGEMELELIELRRALAQTIATFKSTERQRDAQQLIAQRWYEKAQAALDRGNEQLAREALGQRQSYQSHTEALGKSLGEQRALVEQVRGQLQKLERKYLELKSQKNLYLARLKSAIAAQKIEEIAGNLDNASASSLFERIETKILELEAERELLNPPPSPLDKKFEQWEEQQAVEATLAAMKARRSLPPPSS*"
     CDS             complement(2748..3419)
                     /label="Translation 3419-2748"
                     /translation="MGSSHHHHHHSSSAALEVLFQGPAPEDLLERLLGEMELELIELRRALAQTIATFKSTERQRDAQQLIAQRWYEKAQAALDRGNEQLAREALGQRQSYQSHTEALGKSLGEQRALVEQVRGQLQKLERKYLELKSQKNLYLARLKSAIAAQKIEEIAGNLDNASASSLFERIETKILELEAERELLNPPPSPLDKKFEQWEEQQAVEATLAAMKARRSLPPPSS*"
     primer          complement(3329..3350)
                     /label="pET50b SynPspA REV(delH0)"
                     /note="sequence: AAGTCCTCTTTCAGGGACCCgctccggaagatctgttggagc"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          3351..3370
                     /label="pET-50del_FWD(delH0)"
                     /note="sequence: tccaacagatcttccggagcGGGTCCCTGAAAGAGGACTT"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             complement(3351..3374)
                     /label="HRV 3C site"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             complement(3390..3407)
                     /label="6xHis"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             complement(3417..3419)
                     /label="ATG"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     RBS             3428..3433
                     /label="RBS"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     protein_bind    3464..3488
                     /label="lac operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     promoter        complement(3489..3507)
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        3820..3897
                     /label="lacI promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             3898..4980
                     /label="lacI"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     CDS             5552..5743
                     /label="rop"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
ORIGIN
        1 AGGGCGCGTC AGCGGGTGTT GGCGGGTGTC GGGGCGCAGC CATGACCCAG TCACGTAGCG
       61 ATAGCGGAGT GTATACTGGC TTAACTATGC GGCATCAGAG CAGATTGTAC TGAGAGTGCA
      121 CCATATATGC GGTGTGAAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG CATCAGGCGC
      181 TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA
      241 TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG
      301 AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG
      361 TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG
      421 TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG
      481 CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA
      541 AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC
      601 TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT
      661 AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT
      721 GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG
      781 CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT
      841 ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT
      901 GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT
      961 TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG
     1021 GTCATGAACA ATAAAACTGT CTGCTTACAT AAACAGTAAT ACAAGGGGTG TTATGAGCCA
     1081 TATTCAACGG GAAACGTCTT GCTCTAGGCC GCGATTAAAT TCCAACATGG ATGCTGATTT
     1141 ATATGGGTAT AAATGGGCTC GCGATAATGT CGGGCAATCA GGTGCGACAA TCTATCGATT
     1201 GTATGGGAAG CCCGATGCGC CAGAGTTGTT TCTGAAACAT GGCAAAGGTA GCGTTGCCAA
     1261 TGATGTTACA GATGAGATGG TCAGACTAAA CTGGCTGACG GAATTTATGC CTCTTCCGAC
     1321 CATCAAGCAT TTTATCCGTA CTCCTGATGA TGCATGGTTA CTCACCACTG CGATCCCCGG
     1381 CAAAACAGCA TTCCAGGTAT TAGAAGAATA TCCTGATTCA GGTGAAAATA TTGTTGATGC
     1441 GCTGGCAGTG TTCCTGCGCC GGTTGCATTC GATTCCTGTT TGTAATTGTC CTTTTAACAG
     1501 TGATCGCGTA TTTCGTCTCG CTCAGGCGCA ATCACGAATG AATAACGGTT TGGTTGATGC
     1561 GAGTGATTTT GATGACGAGC GTAATGGCTG GCCTGTTGAA CAAGTCTGGA AAGAAATGCA
     1621 TAAACTTTTG CCATTCTCAC CGGATTCAGT CGTCACTCAT GGTGATTTCT CACTTGATAA
     1681 CCTTATTTTT GACGAGGGGA AATTAATAGG TTGTATTGAT GTTGGACGAG TCGGAATCGC
     1741 AGACCGATAC CAGGATCTTG CCATCCTATG GAACTGCCTC GGTGAGTTTT CTCCTTCATT
     1801 ACAGAAACGG CTTTTTCAAA AATATGGTAT TGATAATCCT GATATGAATA AATTGCAGTT
     1861 TCATTTGATG CTCGATGAGT TTTTCTAAGA ATTAATTCAT GAGCGGATAC ATATTTGAAT
     1921 GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG
     1981 AAATTGTAAA CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT
     2041 TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA TAGACCGAGA
     2101 TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC GTGGACTCCA
     2161 ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA CCATCACCCT
     2221 AATCAAGTTT TTTGGGGTCG AGGTGCCGTA AAGCACTAAA TCGGAACCCT AAAGGGAGCC
     2281 CCCGATTTAG AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA GGGAAGAAAG
     2341 CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC GTAACCACCA
     2401 CACCCGCCGC GCTTAATGCG CCGCTACAGG GCGCGTCCCA TTCGCCAATC CGGATATAGT
     2461 TCCTCCTTTC AGCAAAAAAC CCCTCAAGAC CCGTTTAGAG GCCCCAAGGG GTTATGCTAG
     2521 TTATTGCTCA GCGGTGGCAG CAGCCAACTC AGCTTCCTTT CGGGCTTTGT TTAGCAGCCT
     2581 AGGTTAATTA AGCCTCGAGA GCAGCAGAAG TAGAGCTGTC CATGTGCTGG CGTTCGAATT
     2641 TAGCAGCAGC GGTTTCTTTA CTACCGCGTG GCACCAGAGC GAGCTCTGCG GCCGCAAGCT
     2701 TGTCGACGGA CGTCGGGCGC GCCAAGGCCT GTACAGAATT CGGATCCtta cgagcttggc
     2761 ggcggtaagc tacggcgcgc tttcatggca gccagggttg cttccacagc ttgttgctct
     2821 tcccactgct cgaatttttt gtccagcggg gacggaggcg gattcaacag ttcacgttcg
     2881 gcctctaact ccaggatttt ggtttcaatc cgttcgaaca gagagcttgc gctcgcgttg
     2941 tccaggttac ccgcgatctc ctcgatcttc tgcgcagcaa ttgctgactt caggcgggcc
     3001 agataaaggt tcttctggct cttaagctcc aggtatttac gctccagctt ttgcagctgg
     3061 cctctaacct gctcgaccaa tgcacgttgt tcacccagag atttgccgag cgcttcggtg
     3121 tgggactgat agctctgacg ctgacccagc gcttcacgag ctagttgctc attaccacgg
     3181 tccaacgccg cctgcgcctt ttcgtaccaa cgttgggcaa tcagctgttg cgcatcacgt
     3241 tggcgctcag tgcttttaaa cgtcgcaatg gtctgcgcca gtgcgcgacg cagctcgatc
     3301 agctccaatt ccatttcgcc cagcaggcgc tccaacagat cttccggagc GGGTCCCTGA
     3361 AAGAGGACTT CAAGAGCCGC GGAACTAGAA TGGTGATGGT GGTGATGGCT GCTGCCCATA
     3421 TGTATATCTC CTTCTTAAAG TTAAACAAAA TTATTTCTAG AGGGGAATTG TTATCCGCTC
     3481 ACAATTCCCC TATAGTGAGT CGTATTAATT TCGCGGGATC GAGATCGATC TCGATCCTCT
     3541 ACGCCGGACG CATCGTGGCC GGCATCACCG GCGCCACAGG TGCGGTTGCT GGCGCCTATA
     3601 TCGCCGACAT CACCGATGGG GAAGATCGGG CTCGCCACTT CGGGCTCATG AGCGCTTGTT
     3661 TCGGCGTGGG TATGGTGGCA GGCCCCGTGG CCGGGGGACT GTTGGGCGCC ATCTCCTTGC
     3721 ATGCACCATT CCTTGCGGCG GCGGTGCTCA ACGGCCTCAA CCTACTACTG GGCTGCTTCC
     3781 TAATGCAGGA GTCGCATAAG GGAGAGCGTC GAGATCCCGG ACACCATCGA ATGGCGCAAA
     3841 ACCTTTCGCG GTATGGCATG ATAGCGCCCG GAAGAGAGTC AATTCAGGGT GGTGAATGTG
     3901 AAACCAGTAA CGTTATACGA TGTCGCAGAG TATGCCGGTG TCTCTTATCA GACCGTTTCC
     3961 CGCGTGGTGA ACCAGGCCAG CCACGTTTCT GCGAAAACGC GGGAAAAAGT GGAAGCGGCG
     4021 ATGGCGGAGC TGAATTACAT TCCCAACCGC GTGGCACAAC AACTGGCGGG CAAACAGTCG
     4081 TTGCTGATTG GCGTTGCCAC CTCCAGTCTG GCCCTGCACG CGCCGTCGCA AATTGTCGCG
     4141 GCGATTAAAT CTCGCGCCGA TCAACTGGGT GCCAGCGTGG TGGTGTCGAT GGTAGAACGA
     4201 AGCGGCGTCG AAGCCTGTAA AGCGGCGGTG CACAATCTTC TCGCGCAACG CGTCAGTGGG
     4261 CTGATCATTA ACTATCCGCT GGATGACCAG GATGCCATTG CTGTGGAAGC TGCCTGCACT
     4321 AATGTTCCGG CGTTATTTCT TGATGTCTCT GACCAGACAC CCATCAACAG TATTATTTTC
     4381 TCCCATGAAG ACGGTACGCG ACTGGGCGTG GAGCATCTGG TCGCATTGGG TCACCAGCAA
     4441 ATCGCGCTGT TAGCGGGCCC ATTAAGTTCT GTCTCGGCGC GTCTGCGTCT GGCTGGCTGG
     4501 CATAAATATC TCACTCGCAA TCAAATTCAG CCGATAGCGG AACGGGAAGG CGACTGGAGT
     4561 GCCATGTCCG GTTTTCAACA AACCATGCAA ATGCTGAATG AGGGCATCGT TCCCACTGCG
     4621 ATGCTGGTTG CCAACGATCA GATGGCGCTG GGCGCAATGC GCGCCATTAC CGAGTCCGGG
     4681 CTGCGCGTTG GTGCGGACAT CTCGGTAGTG GGATACGACG ATACCGAAGA CAGCTCATGT
     4741 TATATCCCGC CGTTAACCAC CATCAAACAG GATTTTCGCC TGCTGGGGCA AACCAGCGTG
     4801 GACCGCTTGC TGCAACTCTC TCAGGGCCAG GCGGTGAAGG GCAATCAGCT GTTGCCCGTC
     4861 TCACTGGTGA AAAGAAAAAC CACCCTGGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG
     4921 TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA
     4981 GCGCAACGCA ATTAATGTAA GTTAGCTCAC TCATTAGGCA CCGGGATCTC GACCGATGCC
     5041 CTTGAGAGCC TTCAACCCAG TCAGCTCCTT CCGGTGGGCG CGGGGCATGA CTAGCATGAT
     5101 CGTGCTCCTG TCGTTGAGGA CCCGGCTAGG CTGGCGGGGT TGCCTTACTG GTTAGCAGAA
     5161 TGAATCACCG ATACGCGAGC GAACGTGAAG CGACTGCTGC TGCAAAACGT CTGCGACCTG
     5221 AGCAACAACA TGAATGGTCT TCGGTTTCCG TGTTTCGTAA AGTCTGGAAA CGCGGAAGTC
     5281 AGCGCCCTGC ACCATTATGT TCCGGATCTG CATCGCAGGA TGCTGCTGGC TACCCTGTGG
     5341 AACACCTACA TCTGTATTAA CGAAGCGCTG GCATTGACCC TGAGTGATTT TTCTCTGGTC
     5401 CCGCCGCATC CATACCGCCA GTTGTTTACC CTCACAACGT TCCAGTAACC GGGCATGTTC
     5461 ATCATCAGTA ACCCGTATCG TGAGCATCCT CTCTCGTTTC ATCGGTATCA TTACCCCCAT
     5521 GAACAGAAAT CCCCCTTACA CGGAGGCATC AGTGACCAAA CAGGAAAAAA CCGCCCTTAA
     5581 CATGGCCCGC TTTATCAGAA GCCAGACATT AACGCTTCTG GAGAAACTCA ACGAGCTGGA
     5641 CGCGGATGAA CAGGCAGACA TCTGTGAATC GCTTCACGAC CACGCTGATG AGCTTTACCG
     5701 CAGCTGCCTC GCGCGTTTCG GTGATGACGG TGAAAACCTC TGACACATGC AGCTCCCGGA
     5761 GACGGTCACA GCTTGTCTGT AAGCGGATGC CGGGAGCAGA CAAGCCCGTC
//