LOCUS       pILFPZ1A9               7726 bp    DNA     circular     22-Feb-2024
FEATURES             Location/Qualifiers
     misc_feature    2425..3285
                     /label=bla
                     /note=misc_feature
     CDS             4057..4860
                     /label=KlURA3
                     /note=Detected CDS 267aa
     rep_origin      1651..2270
                     /label=rep
                     /note=rep_origin
     promoter        3891..4056
                     /label=URA3p
                     /note=promoter
     misc_feature    1035..1235
                     /label=URA3(705, 905)
                     /note=misc_feature
     terminator      729..1028
                     /label=PGK1 terminator
                     /note=terminator
     promoter        6378..7006
                     /label=TEF1 p
                     /note=promoter
     CDS             1..711
                     /label=mNeonGreen
                     /note=CDS
     misc_feature    5027..5066
                     /label=A
                     /note=misc_feature
     misc_feature    6203..6242
                     /label=A
                     /note=misc_feature
     misc_feature    5073..5112
                     /label=B
                     /note=misc_feature
     misc_feature    6249..6288
                     /label=B
                     /note=misc_feature
     misc_feature    5113..5142
                     /label=I-SceI
                     /note=misc_feature
     misc_feature    6173..6202
                     /label=I-SceI
                     /note=misc_feature
     misc_feature    5143..5526
                     /label=TEFp
                     /note=TEF1 promoter of Ashbya gossypii
     CDS             5528..5908
                     /label=ble
                     /note=ble gene from Tn5, conferring resistance to bleomycin and phleomycin
     misc_feature    5915..6172
                     /label=TEFt
                     /note=TEF1 terminator of Ashbya gossypii
     CDS             7016..7726
                     /label=mTagBFP2 (no stop codon)
                     /note=CDS
BASE COUNT     2130 a      1711 c      1738 g      2147 t 
ORIGIN
        1 ATGGTGTCTA AAGGTGAAGA GGATAACATG GCTTCTTTGC CAGCTACTCA TGAATTGCAT 
       61 ATTTTCGGTT CCATCAACGG TGTTGATTTC GATATGGTTG GTCAAGGTAC TGGTAATCCA 
      121 AATGATGGTT ACGAAGAGTT GAACCTGAAA TCTACAAAAG GTGACTTGCA ATTCTCCCCA 
      181 TGGATTTTGG TTCCACATAT TGGTTACGGT TTCCACCAAT ATTTGCCATA TCCAGATGGT 
      241 ATGTCTCCAT TTCAAGCTGC TATGGTTGAT GGTTCTGGTT ACCAAGTTCA TAGAACCATG 
      301 CAATTTGAAG ATGGTGCTTC TTTGACCGTC AACTACAGAT ATACTTACGA AGGTTCCCAT 
      361 ATTAAGGGTG AAGCTCAAGT AAAAGGTACA GGTTTTCCAG CTGATGGTCC AGTTATGACT 
      421 AATTCTTTGA CTGCTGCTGA TTGGTGCAGA TCCAAGAAAA CTTATCCAAA CGATAAGACC 
      481 ATCATCTCCA CCTTTAAGTG GTCTTACACT ACTGGTAACG GTAAAAGATA CAGATCCACT 
      541 GCTAGAACTA CTTACACTTT TGCTAAACCT ATGGCTGCCA ACTACTTGAA GAATCAACCT 
      601 ATGTACGTTT TCCGTAAGAC CGAATTGAAA CACTCCAAAA CTGAGCTGAA CTTCAAAGAA 
      661 TGGCAAAAGG CTTTCACTGA TGTTATGGGT ATGGATGAGT TGTACAAGTA ATAAAGATCT 
      721 GATCTTAAAT TGAATTGAAT TGAAATCGAT AGATCAATTT TTTTCTTTTC TCTTTCCCCA 
      781 TCCTTTACGC TAAAATAATA GTTTATTTTA TTTTTTGAAT ATTTTTTATT TATATACGTA 
      841 TATATAGACT ATTATTTATC TTTTAATGAT TATTAAGATT TTTATTAAAA AAAAATTCGC 
      901 TCCTCTTTTA ATGCCTTTAT GCAGTTTTTT TTTCCCATTC GATATTTCTA TGTTCGGGTT 
      961 CAGCGTATTT TAAGTTTAAT AACTCGAAAA TTCTGCGTTC GTTAAAGCTT TCGAGAAGGA 
     1021 TATTATTTAC TAGTAGGACT ATTTGCAAAG GGAAGGGATG CTAAGGTAGA GGGTGAACGT 
     1081 TACAGAAAAG CAGGCTGGGA AGCATATTTG AGAAGATGCG GCCAGCAAAA CTAAAAAACT 
     1141 GTATTATAAG TAAATGCATG TATACTAAAC TCACAAATTA GAGCTTCAAT TTAATTATAT 
     1201 CAGTTATTAC CCGGGAATCT CGGTCGTAAT GATTTAAATG CATGCAAGCT TGGCGTAATC 
     1261 ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG 
     1321 AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT 
     1381 TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG 
     1441 AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT 
     1501 CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     1561 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     1621 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     1681 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     1741 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     1801 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     1861 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     1921 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     1981 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     2041 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     2101 TAGAAGGACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     2161 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     2221 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     2281 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     2341 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     2401 ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC 
     2461 GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT 
     2521 ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC 
     2581 GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC 
     2641 TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG 
     2701 TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG 
     2761 CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG 
     2821 ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG 
     2881 TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT 
     2941 CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA 
     3001 ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC 
     3061 ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC 
     3121 AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC 
     3181 TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC 
     3241 CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA 
     3301 ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT 
     3361 TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGT 
     3421 CTAAGAAACC ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT 
     3481 TCGTCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC 
     3541 GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC 
     3601 GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG 
     3661 AGTGCACCAT ATGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT ACCGCATCAG 
     3721 GCGCCATTCG CCATTCAGGC TGCGCAACTG TTGGGAAGGG CGATCGGTGC GGGCCTCTTC 
     3781 GCTATTACGC CAGCTGGCGA AAGGGGGATG TGCTGCAAGG CGATTAAGTT GGGTAACGCC 
     3841 AGGGTTTTCC CAGTCACGAC GTTGTAAAAC GACGGCCAGT GAATTCATTT AAATTTTTTT 
     3901 GATTCGGTAA TCTCCGAGCA GAAGGAAGAA CGAAGGAAGG AGCACAGACT TAGATTGGTA 
     3961 TATATACGCA TATGTGGTGT TGAAGAAACA TGAAATTGCC CAGTATTCTT AACCCAACTG 
     4021 CACAGAACAA AAACCTGCAG GAAACGAAGA TAAAACATGT CCACAAAATC ATATACCAGT 
     4081 AGAGCTGAGA CTCATGCAAG TCCGGTTGCA TCGAAACTTT TACGTTTAAT GGATGAAAAG 
     4141 AAGACCAATT TGTGTGCTTC TCTTGACGTT CGTTCGACTG ATGAGCTATT GAAACTTGTT 
     4201 GAAACGTTGG GTCCATACAT TTGCCTTTTG AAAACACACG TTGATATCTT GGATGATTTC 
     4261 AGTTATGAGG GTACTGTCGT TCCATTGAAA GCATTGGCAG AGAAATACAA GTTCTTGATA 
     4321 TTTGAGGACA GAAAATTCGC CGATATCGGT AACACAGTCA AATTACAATA TACATCGGGC 
     4381 GTTTACCGTA TCGCAGAATG GTCTGATATC ACCAACGCCC ACGGGGTTAC TGGTGCTGGT 
     4441 ATTGTTGCTG GCTTGAAACA AGGTGCGCAA GAGGTCACCA AAGAACCAAG GGGATTATTG 
     4501 ATGCTTGCTG AATTGTCTTC CAAGGGTTCT CTAGCACACG GTGAATATAC TAAGGGTACC 
     4561 GTTGATATTG CAAAGAGTGA TAAAGATTTC GTTATTGGGT TCATTGCTCA GAACGATATG 
     4621 GGAGGAAGAG AAGAAGGGTT TGATTGGCTA ATCATGACCC CAGGTGTAGG TTTAGACGAC 
     4681 AAAGGCGATG CATTGGGTCA GCAGTACAGA ACCGTCGACG AAGTTGTAAG TGGTGGATCA 
     4741 GATATCATCA TTGTTGGCAG AGGACTTTTC GCCAAGGGTA GAGATCCTAA GGTTGAAGGT 
     4801 GAAAGATACA GAAATGCTGG ATGGGAAGCG TACCAAAAGA GAATCAGCGC TCCCCATTAA 
     4861 TTATACAGGA AACTTAATAG AACAAATCAC ATATTTAATC TAATAGCCAC CTGCATTGGC 
     4921 ACGGTGCAAC ACTCACTTCA ACTTCATCTT ACAAAAGATC ACGTGATCTG TTGTATTGGG 
     4981 ATCTCTAGGC TGAAGCTTCG TACGCTGCAG GTCGACAACC CTTAATGTTC TTCATTCTAC 
     5041 GCACGGTCCT TCAATTCGCT TGGCGGAGAT CTATGACAAG AGGGTCGAAC TCGCCTAAGT 
     5101 CGTAATTGAG TCAGTTACGC TAGGGATAAC AGGGTAATAT AGCTGTTTAG CTTGCCTCGT 
     5161 CCCCGCCGGG TCACCCGGCC AGCGACATGG AGGCCCAGAA TACCCTCCTT GACAGTCTTG 
     5221 ACGTGCGCAG CTCAGGGGCA TGATGTGACT GTCGCCCGTA CATTTAGCCC ATACATCCCC 
     5281 ATGTATAATC ATTTGCATCC ATACATTTTG ATGGCCGCAC GGCGCGAAGC AAAAATTACG 
     5341 GCTCCTCGCT GCAGACCTGC GAGCAGGGAA ACGCTCCCCT CACAGACGCG TTGAATTGTC 
     5401 CCCACGCCGC GCCCCTGTAG AGAAATATAA AAGGTTAGGA TTTGCCACTG AGGTTCTTCT 
     5461 TTCATATACT TCCTTTTAAA ATCTTGCTAG GATACAGTTC TCACATCACA TCCGAACATA 
     5521 AACAACCATG GCCGACCAAG CGACGCCCAA CCTGCCATCA CGAGATTTCG ATTCCACGGC 
     5581 CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT 
     5641 CCAGCGCGGG GATCTCAAGC TGGAGTTCTT CGCCCACCCC GGGCTCGATC CCCTCGCGAG 
     5701 TTGGTTCAGC TGCTGCCTGA GGCTGGACGA CCTCGCGGAG TTCTACCGGC AGTGCAAATC 
     5761 CGTCGGCATC CAGGAAACCA GCAGCGGCTA TCCGCGCATC CATGCCCCCG AACTGCAGGA 
     5821 GTGGGGAGGC ACGATGGCCG CTTTGGTCGA CCCGGACGGG ACGCTCCTGC GCCTGATACA 
     5881 GAACGAATTG CTTGCAGGCA TCTCATGATC AGTACTGACA ATAAAAAGAT TCTTGTTTTC 
     5941 AAGAACTTGT CATTTGTATA GTTTTTTTAT ATTGTAGTTG TTCTATTTTA ATCAAATGTT 
     6001 AGCGTGATTT ATATTTTTTT TCGCCTCGAC ATCATCTGCC CAGATGCGAA GTTAAGTGCG 
     6061 CAGAAAGTAA TATCATGCGT CAATCGTATG TGAATGCTGG TCGCTATACT GCTGTCGATT 
     6121 CGATACTAAC GCCGCCATCC AGTGTCGAAA ACGAGCTCTC GAGAACCCTT AAAGTTACGC 
     6181 TAGGGATAAC AGGGTAATAT AGGTTCTTCA TTCTACGCAC GGTCCTTCAA TTCGCTTGGC 
     6241 GGTCTAGAAT GACAAGAGGG TCGAACTCGC CTAAGTCGTA ATTGAGTCCA GATCCACTAG 
     6301 TGGCCTATCT AGACCTAATA ACTTCGTATA GCATACATTA TACGAAGTTA TATTAAGGGT 
     6361 TGCTCGAGAA AGAGCTCCAG AAAGCGACCA CCCAACTTTG GCTGATAATA GCGTATAAAC 
     6421 AATGCATACT TTGTACGTTC AAAATACAAT GCAGTAGATA TATTTATGCA TATTACATAT 
     6481 AATACATATC ACATAGGAAG CAACAGGCGC GTTGGACTTT TAATTTTCGA GGACCGCGAA 
     6541 TCCTTACATC ACACCCAATC CCCCACAAGT GATCCCCCAC ACACCATAGC TTCAAAATGT 
     6601 TTCTACTCCT TTTTTACTCT TCCAGATTTT CTCGGACTCC GCGCATCGCC GTACCACTTC 
     6661 AAAACACCCA AGCACAGCAT ACTAAATTTC CCCTCTTTCT TCCTCTAGGG TGTCGTTAAT 
     6721 TACCCGTACT AAAGGTTTGG AAAAGAAAAA AGAGACCGCC TCGTTTCTTT TTCTTCGTCG 
     6781 AAAAAGGCAA TAAAAATTTT TATCACGTTT CTTTTTCTTG AAAATTTTTT TTTTTGATTT 
     6841 TTTTCTCTTT CGATGACCTC CCATTGATAT TTAAGTTAAT AAACGGTCTT CAATTTCTCA 
     6901 AGTTTCAGTT TCATTTTTCT TGTTCTATTA CAACTTTTTT TACTTCTTGC TCATTAGAAA 
     6961 GAAAGCATAG CAATCTAATC TAAGTTTTAA TTACAAACTC GAGAAAGGAT CCAAAATGGT 
     7021 GTCTAAGGGT GAAGAGCTGA TCAAAGAAAA CATGCACATG AAGTTGTACA TGGAAGGTAC 
     7081 TGTTGATAAC CATCATTTCA AGTGTACCTC TGAAGGTGAA GGTAAACCAT ACGAAGGTAC 
     7141 TCAAACTATG AGAATCAAGG TTGTTGAAGG TGGTCCATTG CCATTTGCTT TTGATATTTT 
     7201 GGCTACCTCC TTCTTGTACG GTTCTAAGAC TTTCATCAAC CACACTCAAG GTATCCCAGA 
     7261 TTTCTTCAAG CAATCTTTTC CAGAAGGTTT CACTTGGGAA AGAGTTACTA CTTACGAAGA 
     7321 TGGTGGTGTT TTGACTGCTA CTCAAGATAC TTCTTTACAA GACGGTTGCT TGATCTACAA 
     7381 CGTTAAGATT AGAGGTGTCA ACTTCACTTC TAACGGTCCA GTTATGCAAA AGAAAACTTT 
     7441 AGGTTGGGAA GCCTTCACCG AAACATTATA TCCAGCTGAT GGTGGTTTGG AAGGTAGAAA 
     7501 TGATATGGCT TTGAAATTGG TTGGTGGCTC CCATTTGATT GCTAACGCTA AGACTACCTA 
     7561 CAGGTCTAAG AAGCCAGCTA AGAATTTGAA AATGCCAGGT GTTTACTACG TGGACTATAG 
     7621 ATTGGAAAGA ATCAAAGAAG CCAACAACGA AACCTACGTT GAACAACACG AAGTTGCTGT 
     7681 TGCTAGATAT TGTGATTTGC CATCAAAGTT GGGTCACAAG TTGAAC 
//