LOCUS       HCT7_pAAV-eHGT_1142m-minBglobin-SYFP2-WPRE3-bGHpA        4966 bp    DNA     circular UNA 09-JUL-2024
DEFINITION  Gibson Cloning Product .
ACCESSION   urn.local...gci-b00ahia
VERSION     urn.local...gci-b00ahia
KEYWORDS    .
SOURCE      
  ORGANISM  Mouse.
FEATURES             Location/Qualifiers
     misc_feature    1..130
                     /vntifkey=21
                     /locus_tag="L-ITR"
                     /ApEinfo_label="L-ITR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="L-ITR"
     misc_feature    46^47
                     /Original_Bases="AAAGCCCGGGC"
                     /label="AAAGCCCGGGC"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    complement(126..908)
                     /Source="referenceToDocument: urn:local:.:5x-hwrhcx4"
                     /label="& Trimmed m462-RMSYFP2seqR.ab1 (concatenated
                     sequence 2) (reversed)"
                     /note="Geneious type: Concatenated sequence"
     enhancer        146..867
                     /created_by="brooke.wynalda"
                     /label="eHGT_1142m_Enhancer"
     primer_bind     146..166
                     /created_by="brooke.wynalda"
                     /Sequence="GGCACACCTAGTCCAACAAAG"
                     /Tm="59.1"
                     /%GC="52.4"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /modified_by="brooke.wynalda"
                     /label="GT_3/GT_51"
     primer_bind     complement(846..867)
                     /created_by="brooke.wynalda"
                     /Sequence="CAGATGGAAGAAGCTCTGACTC"
                     /Tm="58.2"
                     /%GC="50.0"
                     /Hairpin_Tm="37.9"
                     /Self_Dimer_Tm="None"
                     /modified_by="brooke.wynalda"
                     /label="GT_4/GT_52"
     misc_feature    890..143
                     /Source="referenceToDocument: urn:local:.:5w-hwrhcx4"
                     /label="CN1244_pAAV Linearized (digested by SacI, MluI)
                     Fragment 3 (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     promoter        891..943
                     /vntifkey=21
                     /locus_tag="Beta\Globin\minimal\promoter"
                     /ApEinfo_label="Beta\Globin\minimal\promoter"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /modified_by="jacqueline.bendrick"
                     /label="beta-globin minimal promoter"
     misc_feature    900..905
                     /vntifkey=21
                     /locus_tag="TATA\box"
                     /ApEinfo_label="TATA\box"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="TATA\box"
     misc_feature    932
                     /vntifkey=21
                     /locus_tag="transcriptional\start"
                     /ApEinfo_label="transcriptional\start"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="transcriptional\start"
     misc_feature    1738..1983
                     /vntifkey=21
                     /locus_tag="WPRE3"
                     /ApEinfo_label="WPRE3"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="WPRE3"
     polyA_signal    1997..2200
                     /vntifkey=21
                     /locus_tag="BGH-pA"
                     /ApEinfo_label="BGH-pA"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /modified_by="jacqueline.bendrick"
                     /label="bGHpA"
     primer          complement(2111..2130)
                     /vntifkey=27
                     /locus_tag="BGH-R"
                     /ApEinfo_label="BGH-R"
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#80ff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BGH-R"
     misc_feature    2229..2369
                     /vntifkey=21
                     /locus_tag="R-ITR"
                     /ApEinfo_label="R-ITR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="R-ITR"
     rep_origin      2461..2767
                     /vntifkey=33
                     /locus_tag="f1\Origin"
                     /ApEinfo_label="f1\Origin"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /modified_by="jacqueline.bendrick"
                     /label="f1 Origin"
     misc_feature    3286..4143
                     /vntifkey=21
                     /locus_tag="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="AmpR"
     rep_origin      4294..4961
                     /vntifkey=33
                     /locus_tag="pUC\ori"
                     /ApEinfo_label="pUC\ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="pUC\ori"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtaggcac acctagtcca acaaagccat acttcctaat
      181 agtgccactc cctggtacaa gcatatacag agcatcacaa tttgttttct aacatacaga
      241 gttctaacac ggttaatcca gcttgagcac atttctgtca tctcaggatt tgtttctctt
      301 tctggctgca gctggccttt gtccttgatc ccctcccttc cctttggttc tcagctcttc
      361 catcgctgca ttctgctgtc ttggcacttt gccgtgcctt ttgactaata cctgcctctc
      421 tccccagccc ttggcagccc accattctac tactatgagc tcagcatatt acattccttc
      481 cctttatgcc attttaaaag acatgctttc taacacagaa acgctaagat gtaatgtgca
      541 tattgcattg aagttcaggc agcaagaggt gagatatttc agactttctt gttctgagct
      601 gttggagcac ggaacactga ggttggcttt gttcctagca caggagtggc gcttaaatat
      661 caaaccacac tggttttaac attaacatgt ccattagaat atgtcagcat tttcaaacac
      721 cacatatgca aacatgcttc taatgccaag gaagcccaga gtttgacaaa ttatccagac
      781 taagcatcag aattgccaag agaatttaac aaaaaaaaaa agttgatagt ttttctagat
      841 catctgagtc agagcttctt ccatctgact atttcactga gtttgagctc gggctgggca
      901 taaaagtcag ggcagagcca tctattgctt acatttgctt ctgggatcca gatctttcga
      961 agctagcgct accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg
     1021 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     1081 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gctgatctgc accaccggca
     1141 agctgcccgt gccctggccc accctcgtga ccaccctggg ctacggcgtg cagtgcttcg
     1201 cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     1261 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     1321 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     1381 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     1441 tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc cacaacatcg
     1501 aggacggcgg cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     1561 ccgtgctgct gcccgacaac cactacctga gctaccagtc caagctgagc aaagacccca
     1621 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     1681 gcatggacga gctgtacaag taagtcgacg gcgcgccgcg gccgcgaatt cgatatcata
     1741 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     1801 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     1861 tggctttcat tttctcctcc ttgtataaat cctggttagt tcttgccacg gcggaactca
     1921 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     1981 tggctcgaga gatcttcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     2041 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     2101 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     2161 gacagcaagg gggaggattg ggaagacaat agcaggcatg agatctcacg tgcggaccga
     2221 gcggccgcag gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct
     2281 cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt
     2341 gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc tccttacgca
     2401 tctgtgcggt atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc
     2461 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     2521 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     2581 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     2641 gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     2701 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     2761 ggaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat tttgccgatt
     2821 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     2881 atattaacgt ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag
     2941 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     3001 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     3061 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
     3121 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     3181 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     3241 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     3301 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     3361 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     3421 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     3481 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     3541 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     3601 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     3661 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     3721 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     3781 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     3841 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     3901 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     3961 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     4021 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     4081 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     4141 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     4201 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     4261 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     4321 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     4381 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     4441 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     4501 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     4561 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     4621 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     4681 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     4741 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     4801 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     4861 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     4921 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgt
//
