LOCUS       HCT42_pAAV-eHGT_1184m-minBglobin-SYFP2-WPRE3-bGHpA        4881 bp    DNA     circular UNA 09-JUL-2024
DEFINITION  Restriction Cloning Ligation Product .
ACCESSION   urn.local...gci-b00ahia
VERSION     urn.local...gci-b00ahia
KEYWORDS    .
SOURCE      
  ORGANISM  Mouse.
FEATURES             Location/Qualifiers
     misc_feature    1..130
                     /vntifkey=21
                     /locus_tag="L-ITR"
                     /ApEinfo_label="L-ITR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="L-ITR"
     misc_feature    46^47
                     /Original_Bases="AAAGCCCGGGC"
                     /label="AAAGCCCGGGC"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    complement(126..823)
                     /Source="referenceToDocument: urn:local:.:dj-hwrnsre"
                     /label="& Trimmed m462-RMSYFP2seqR.ab1 (concatenated
                     sequence 2) (reversed)"
                     /note="Geneious type: Concatenated sequence"
     Enhancer        145..783
                     /created_by="brooke.wynalda"
                     /modified_by="brooke.wynalda"
                     /label="eGT_1184m_Enhancer"
     primer_bind     145..163
                     /Sequence="GGACAGCTACTTCAGAAGG"
                     /Tm="54.6"
                     /%GC="52.6"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="14.9"
                     /created_by="brooke.wynalda"
                     /label="GT_117"
     primer_bind     complement(764..783)
                     /Sequence="CCCAGCCCCTCAAAGATAGG"
                     /Tm="59.5"
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /created_by="brooke.wynalda"
                     /label="GT_118"
     misc_feature    805..143
                     /Source="referenceToDocument: urn:local:.:di-hwrnsre"
                     /label="CN1244_pAAV Linearized (digested by SacI, MluI)
                     Fragment 3 (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     promoter        806..858
                     /vntifkey=21
                     /locus_tag="Beta\Globin\minimal\promoter"
                     /ApEinfo_label="Beta\Globin\minimal\promoter"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /modified_by="jacqueline.bendrick"
                     /label="beta-globin minimal promoter"
     misc_feature    815..820
                     /vntifkey=21
                     /locus_tag="TATA\box"
                     /ApEinfo_label="TATA\box"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="TATA\box"
     misc_feature    847
                     /vntifkey=21
                     /locus_tag="transcriptional\start"
                     /ApEinfo_label="transcriptional\start"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="transcriptional\start"
     primer_bind     896..914
                     /%GC="63.2"
                     /Hairpin_Tm="47.5"
                     /Self_Dimer_Tm="19.0"
                     /Tm="62.9"
                     /annotation_group="2674114959566006bfd1120-fbec-4fb0-a4ef-
                     1786f2ea2e82"
                     /created_by="primer3"
                     /Sequence="ACCATGGTGAGCAAGGGCG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-791"
     primer_bind     complement(896..914)
                     /%GC="63.2"
                     /Hairpin_Tm="37.1"
                     /Self_Dimer_Tm="13.0"
                     /Tm="62.9"
                     /annotation_group="26741393307789903d436ce-462d-444b-ae29-
                     84b7c64ed2b1"
                     /created_by="primer3"
                     /Sequence="CGCCCTTGCTCACCATGGT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-792"
     primer_bind     896..914
                     /%GC="63.2"
                     /Hairpin_Tm="47.5"
                     /Self_Dimer_Tm="19.0"
                     /Tm="62.9"
                     /annotation_group="268225705991100a1c86835-2d9e-4a94-867d-
                     59d0721fdd51"
                     /created_by="primer3"
                     /Sequence="ACCATGGTGAGCAAGGGCG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-791"
     primer_bind     complement(896..914)
                     /%GC="63.2"
                     /Hairpin_Tm="37.1"
                     /Self_Dimer_Tm="13.0"
                     /Tm="62.9"
                     /annotation_group="268228180191200bfec062e-823a-4689-a151-
                     95c8c50f0fc4"
                     /created_by="primer3"
                     /Sequence="CGCCCTTGCTCACCATGGT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-792"
     CDS             899..1618
                     /kind="reporter"
                     /note="Yellow fluorescent reporter protein - Ex:515,
                     Em:527"
                     /Color="#e1ff2f"
                     /product="SYFP2"
                     /gene="SYFP2"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SYFP2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:dx-enavel9"">SYFP2</a>"
                     /label="SYFP2"
     primer_bind     complement(899..919)
                     /%GC="61.9"
                     /Hairpin_Tm="34.4"
                     /Self_Dimer_Tm="None"
                     /Tm="64.5"
                     /annotation_group="267411313157700ab081476-d455-484b-9f16-
                     f7265b92726c"
                     /created_by="primer3"
                     /Sequence="CTCCTCGCCCTTGCTCACCAT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-2595"
     primer_bind     complement(899..919)
                     /%GC="61.9"
                     /Hairpin_Tm="34.4"
                     /Self_Dimer_Tm="None"
                     /Tm="64.5"
                     /annotation_group="268225471053600fb284203-12a5-4b6f-aa11-
                     a50fa338630d"
                     /created_by="primer3"
                     /Sequence="CTCCTCGCCCTTGCTCACCAT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-2595"
     primer_bind     908..927
                     /%GC="60.0"
                     /Hairpin_Tm="47.3"
                     /Self_Dimer_Tm="None"
                     /Tm="62.5"
                     /annotation_group="2674137644551000debb238-62ff-48b0-84db-
                     2273d95aad00"
                     /created_by="primer3"
                     /Sequence="AAGGGCGAGGAGCTGTTCAC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-667"
     primer_bind     908..927
                     /%GC="60.0"
                     /Hairpin_Tm="47.3"
                     /Self_Dimer_Tm="None"
                     /Tm="62.5"
                     /annotation_group="268228007567900bf107f4b-dace-47cf-9678-
                     9d0ce8c6c327"
                     /created_by="primer3"
                     /Sequence="AAGGGCGAGGAGCTGTTCAC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-667"
     primer_bind     943..962
                     /%GC="75.0"
                     /Hairpin_Tm="37.8"
                     /Self_Dimer_Tm="30.2"
                     /Tm="67.6"
                     /annotation_group="267412029042400bd640d40-19ee-4d12-9e22-
                     213c87aeb9c3"
                     /created_by="primer3"
                     /Sequence="CCTGGTCGAGCTGGACGGCG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-331"
     primer_bind     943..962
                     /%GC="75.0"
                     /Hairpin_Tm="37.8"
                     /Self_Dimer_Tm="30.2"
                     /Tm="67.6"
                     /annotation_group="268226282281000335e9e10-e95a-4203-8c38-
                     08482524df12"
                     /created_by="primer3"
                     /Sequence="CCTGGTCGAGCTGGACGGCG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-331"
     primer_bind     961..983
                     /%GC="52.2"
                     /Hairpin_Tm="46.5"
                     /Self_Dimer_Tm="2.2"
                     /Tm="63.9"
                     /annotation_group="2674124959641004c3bdab9-67bb-4058-8635-
                     cfc5da5dc9be"
                     /created_by="primer3"
                     /Sequence="CGACGTAAACGGCCACAAGTTCA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-736"
     primer_bind     961..983
                     /%GC="52.2"
                     /Hairpin_Tm="46.5"
                     /Self_Dimer_Tm="2.2"
                     /Tm="63.9"
                     /annotation_group="26822674369520051e099fe-a601-405e-9758-
                     e618b8fdca09"
                     /created_by="primer3"
                     /Sequence="CGACGTAAACGGCCACAAGTTCA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-736"
     primer_bind     961..980
                     /%GC="55.0"
                     /Hairpin_Tm="46.5"
                     /Self_Dimer_Tm="2.2"
                     /Tm="61.2"
                     /annotation_group="267414048146100c47d653d-e673-4064-a014-
                     182b5cb2d872"
                     /created_by="primer3"
                     /Sequence="CGACGTAAACGGCCACAAGT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-2645"
     primer_bind     961..980
                     /%GC="55.0"
                     /Hairpin_Tm="46.5"
                     /Self_Dimer_Tm="2.2"
                     /Tm="61.2"
                     /annotation_group="268228293463600a2c515e3-a96e-4e46-b3ef-
                     e4b6ec799334"
                     /created_by="primer3"
                     /Sequence="CGACGTAAACGGCCACAAGT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-2645"
     primer_bind     complement(966..988)
                     /%GC="52.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="64.2"
                     /annotation_group="2674125527081006f937811-e03e-437a-83f0-
                     1772fa76fe10"
                     /created_by="primer3"
                     /Sequence="CACGCTGAACTTGTGGCCGTTTA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-2462"
     primer_bind     complement(966..988)
                     /%GC="52.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="64.2"
                     /annotation_group="26822679981429913c8b7a9-10b5-4fff-acc5-
                     f73c489d02cd"
                     /created_by="primer3"
                     /Sequence="CACGCTGAACTTGTGGCCGTTTA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-2462"
     primer_bind     967..986
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="2.2"
                     /Tm="62.1"
                     /annotation_group="2674122629687991b662f0d-b2ff-4b6f-a40b-
                     2c716e0ece3f"
                     /created_by="primer3"
                     /Sequence="AAACGGCCACAAGTTCAGCG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-1027"
     primer_bind     967..986
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="2.2"
                     /Tm="62.1"
                     /annotation_group="268226512923799f4a38d0a-b6eb-4653-9e50-
                     42272b630244"
                     /created_by="primer3"
                     /Sequence="AAACGGCCACAAGTTCAGCG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-1027"
     primer_bind     complement(1012..1035)
                     /%GC="58.3"
                     /Hairpin_Tm="58.5"
                     /Self_Dimer_Tm="None"
                     /Tm="66.7"
                     /annotation_group="2674123779128000a63cd81-84ab-46e1-ac7e-
                     3e592aa419bb"
                     /created_by="primer3"
                     /Sequence="TTCAGGGTCAGCTTGCCGTAGGTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-598"
     primer_bind     complement(1012..1035)
                     /%GC="58.3"
                     /Hairpin_Tm="58.5"
                     /Self_Dimer_Tm="None"
                     /Tm="66.7"
                     /annotation_group="2682266283519002c557c85-ad28-417d-b2e6-
                     b28f9797a5e7"
                     /created_by="primer3"
                     /Sequence="TTCAGGGTCAGCTTGCCGTAGGTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-598"
     primer_bind     complement(1013..1032)
                     /%GC="60.0"
                     /Hairpin_Tm="58.5"
                     /Self_Dimer_Tm="None"
                     /Tm="63.4"
                     /annotation_group="26741347531369925c9f147-2bed-450a-b081-
                     a5dae9644517;
                     26741353304070092ae149d-f2a5-4953-8fea-69cc724c05e2"
                     /created_by="primer3"
                     /Sequence="AGGGTCAGCTTGCCGTAGGT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-977; MG-160"
     primer_bind     complement(1013..1032)
                     /%GC="60.0"
                     /Hairpin_Tm="58.5"
                     /Self_Dimer_Tm="None"
                     /Tm="63.4"
                     /annotation_group="26822771877980052ff1eaa-1d5d-46da-bfdb-
                     0fefead0e928;
                     2682277748689004255a9d0-f587-47e5-8056-5fc97ef247ab"
                     /created_by="primer3"
                     /Sequence="AGGGTCAGCTTGCCGTAGGT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-977; MG-160"
     primer_bind     1040..1059
                     /%GC="60.0"
                     /Hairpin_Tm="41.4"
                     /Self_Dimer_Tm="None"
                     /Tm="64.9"
                     /annotation_group="26741278488210043a619de-baac-4f09-82de-
                     9299b2274c97"
                     /created_by="primer3"
                     /Sequence="ATCTGCACCACCGGCAAGCT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-135"
     primer_bind     1040..1059
                     /%GC="60.0"
                     /Hairpin_Tm="41.4"
                     /Self_Dimer_Tm="None"
                     /Tm="64.9"
                     /annotation_group="2682270314221993df7db86-a02a-4d68-92d8-
                     56b071fde0f6"
                     /created_by="primer3"
                     /Sequence="ATCTGCACCACCGGCAAGCT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-135"
     primer_bind     1111..1130
                     /%GC="75.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="67.4"
                     /annotation_group="26741167619720041cc659a-68f5-466d-949f-
                     406d63ae464f;
                     267414329676900b9c185a4-3605-4637-a320-93005b784649"
                     /created_by="primer3"
                     /Sequence="CTTCGCCCGCTACCCCGACC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-548; MG-2641"
     primer_bind     1111..1130
                     /%GC="75.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="67.4"
                     /annotation_group="268225937281800eeef4b57-dc1e-4d30-b300-
                     5040c8295f1a;
                     26822858318680039ab94f4-7e3e-4446-b7b3-97b26f94d73c"
                     /created_by="primer3"
                     /Sequence="CTTCGCCCGCTACCCCGACC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-548; MG-2641"
     primer_bind     complement(1120..1139)
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.5"
                     /annotation_group="2674128985969005a169f75-75c7-4008-95eb-
                     179e70fa2507"
                     /created_by="primer3"
                     /Sequence="GCTTCATGTGGTCGGGGTAG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-3143-ES-R"
     primer_bind     complement(1120..1139)
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.5"
                     /annotation_group="268227144880099862bdb6a-5e90-450b-8719-
                     743f8ac05ea8"
                     /created_by="primer3"
                     /Sequence="GCTTCATGTGGTCGGGGTAG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-3143-ES-R"
     primer_bind     1129..1150
                     /%GC="54.5"
                     /Hairpin_Tm="42.2"
                     /Self_Dimer_Tm="None"
                     /Tm="62.3"
                     /annotation_group="2674136475308003a9a5f3e-4337-4fb4-9d58-
                     2199b166c803"
                     /created_by="primer3"
                     /Sequence="CCACATGAAGCAGCACGACTTC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-2836-MM-F"
     primer_bind     1129..1150
                     /%GC="54.5"
                     /Hairpin_Tm="42.2"
                     /Self_Dimer_Tm="None"
                     /Tm="62.3"
                     /annotation_group="2682278891931990ee60755-286b-4400-a608-
                     49d44f437a6f"
                     /created_by="primer3"
                     /Sequence="CCACATGAAGCAGCACGACTTC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-2836-MM-F"
     primer_bind     1130..1149
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.1"
                     /annotation_group="267411738546499b3885738-5cf7-45f5-b70f-
                     a0e6037f3066"
                     /created_by="primer3"
                     /Sequence="CACATGAAGCAGCACGACTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-129"
     primer_bind     1130..1149
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.1"
                     /annotation_group="2682259951647005c2624f4-3104-49ab-81d7-
                     b3ff611ea54a"
                     /created_by="primer3"
                     /Sequence="CACATGAAGCAGCACGACTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-129"
     primer_bind     complement(1174..1197)
                     /%GC="58.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="27.2"
                     /Tm="66.5"
                     /annotation_group="26741382075440072520115-a4f7-4215-a874-
                     3d463d9bf95b"
                     /created_by="primer3"
                     /Sequence="AAGATGGTGCGCTCCTGGACGTAG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-158"
     primer_bind     complement(1174..1197)
                     /%GC="58.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="27.2"
                     /Tm="66.5"
                     /annotation_group="2682280652442004a9ec3cb-860b-40ae-b470-
                     1cce0714b1a3"
                     /created_by="primer3"
                     /Sequence="AAGATGGTGCGCTCCTGGACGTAG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-158"
     primer_bind     1180..1197
                     /%GC="61.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="26.0"
                     /Tm="59.7"
                     /annotation_group="2674144440330999cf47a6a-d778-4bc0-864e-
                     5dcddc7ac10d"
                     /created_by="primer3"
                     /Sequence="CCAGGAGCGCACCATCTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-111"
     primer_bind     1180..1197
                     /%GC="61.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="26.0"
                     /Tm="59.7"
                     /annotation_group="2682287001684009447d0f6-b139-4dd5-9a48-
                     673a5138554a"
                     /created_by="primer3"
                     /Sequence="CCAGGAGCGCACCATCTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-111"
     primer_bind     complement(1181..1203)
                     /%GC="52.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="27.2"
                     /Tm="63.3"
                     /annotation_group="2674113730263007bb01a25-0cf9-4884-bf4e-
                     2f6763f2e498;
                     2674132447892004aa5b78d-4b12-435b-8296-030ef25cd804"
                     /created_by="primer3"
                     /Sequence="TTGAAGAAGATGGTGCGCTCCTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-777; MG-2467"
     primer_bind     complement(1181..1203)
                     /%GC="52.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="27.2"
                     /Tm="63.3"
                     /annotation_group="2682255293740991c69ecfe-e739-4ec2-8d99-
                     f68330a4abfd;
                     268227489622999d3e6423a-7c81-4fab-8918-133accaf46be"
                     /created_by="primer3"
                     /Sequence="TTGAAGAAGATGGTGCGCTCCTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-777; MG-2467"
     primer_bind     1197..1216
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="1.6"
                     /Tm="60.6"
                     /annotation_group="2674133020390993b496e9d-a602-4a72-9d4b-
                     54ae379a5fc2"
                     /created_by="primer3"
                     /Sequence="TCTTCAAGGACGACGGCAAC"
                     /Mismatches=0
                     /#_Off-target_Sites=1
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA<br>"
                     /Off-target_#1="<html><font
                     color=""red""><b>A</b></font>CTTCAAGGA<font
                     color=""red""><b>G</b></font>GACGGCAAC</html>"
                     /Location_#1="<html>1,339 -&gt; 1,358 (AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA)</html>"
                     /label="MG-2709"
     primer_bind     1197..1216
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="1.6"
                     /Tm="60.6"
                     /annotation_group="268227546899900707ec0b3-fe17-47a1-ac82-
                     dcc31350e42c"
                     /created_by="primer3"
                     /Sequence="TCTTCAAGGACGACGGCAAC"
                     /Mismatches=0
                     /#_Off-target_Sites=1
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA<br>"
                     /Off-target_#1="<html><font
                     color=""red""><b>A</b></font>CTTCAAGGA<font
                     color=""red""><b>G</b></font>GACGGCAAC</html>"
                     /Location_#1="<html>1,339 -&gt; 1,358 (AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA)</html>"
                     /label="MG-2709"
     primer_bind     1214..1233
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="64.3"
                     /annotation_group="26741161711820049095d55-cfe9-47c3-b28b-
                     867056dfbd2a"
                     /created_by="primer3"
                     /Sequence="AACTACAAGACCCGCGCCGA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-627"
     primer_bind     1214..1233
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="64.3"
                     /annotation_group="268225822236299ede3a2d2-7b40-457a-b132-
                     a58a31178ac0"
                     /created_by="primer3"
                     /Sequence="AACTACAAGACCCGCGCCGA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-627"
     primer_bind     complement(1232..1251)
                     /%GC="60.0"
                     /Hairpin_Tm="42.4"
                     /Self_Dimer_Tm="None"
                     /Tm="61.9"
                     /annotation_group="2674134183220000d7df526-d7f2-431e-902b-
                     bf945b0500fe"
                     /created_by="primer3"
                     /Sequence="TCGCCCTCGAACTTCACCTC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-62"
     primer_bind     complement(1232..1251)
                     /%GC="60.0"
                     /Hairpin_Tm="42.4"
                     /Self_Dimer_Tm="None"
                     /Tm="61.9"
                     /annotation_group="268227662973599eb4edfb3-2f79-4399-a647-
                     c36fdb05d642"
                     /created_by="primer3"
                     /Sequence="TCGCCCTCGAACTTCACCTC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-62"
     primer_bind     1285..1304
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="62.2"
                     /annotation_group="2674130725738006e5fc539-a586-425e-9d20-
                     a708e48fbaa4"
                     /created_by="primer3"
                     /Sequence="CGACTTCAAGGAGGACGGCA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-144"
     primer_bind     1285..1304
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="62.2"
                     /annotation_group="26822731525589972790ab7-303b-43c6-bf49-
                     0b1064dc0478"
                     /created_by="primer3"
                     /Sequence="CGACTTCAAGGAGGACGGCA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-144"
     primer_bind     1313..1332
                     /%GC="55.0"
                     /Hairpin_Tm="41.7"
                     /Self_Dimer_Tm="None"
                     /Tm="60.3"
                     /annotation_group="267414387800999594dca57-9329-4ad1-8638-
                     89a44afc8958"
                     /created_by="primer3"
                     /Sequence="GGGCACAAGCTGGAGTACAA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-3140-ES-F"
     primer_bind     1313..1332
                     /%GC="55.0"
                     /Hairpin_Tm="41.7"
                     /Self_Dimer_Tm="None"
                     /Tm="60.3"
                     /annotation_group="26822864026620046a6d84c-d712-456a-81fc-
                     f011d2d94421"
                     /created_by="primer3"
                     /Sequence="GGGCACAAGCTGGAGTACAA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-3140-ES-F"
     primer_bind     1327..1346
                     /%GC="45.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="55.6"
                     /annotation_group="267411854696300d78eb1c8-8e39-4cad-a32f-
                     c93910a9f3fe"
                     /created_by="primer3"
                     /Sequence="GTACAACTACAACAGCCACA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-668"
     primer_bind     1327..1346
                     /%GC="45.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="55.6"
                     /annotation_group="268226111931500efa4420b-5557-45e1-b25d-
                     44c3d2858256"
                     /created_by="primer3"
                     /Sequence="GTACAACTACAACAGCCACA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-668"
     primer_bind     1344..1363
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="57.5"
                     /annotation_group="267412204882500787da284-21cb-489f-8d7b-
                     0b85eac53fa1"
                     /created_by="primer3"
                     /Sequence="ACAACGTCTATATCACCGCC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-332"
     primer_bind     1344..1363
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="57.5"
                     /annotation_group="2682264547963999dd023bd-9bce-4c3f-81b4-
                     da593d987cec"
                     /created_by="primer3"
                     /Sequence="ACAACGTCTATATCACCGCC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-332"
     primer_bind     1393..1415
                     /%GC="52.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="62.3"
                     /annotation_group="267411796101100c06fe419-583b-411b-856c-
                     860c017d311f;
                     26741260922340067be3c63-c47d-47e6-b838-fab263ec1655"
                     /created_by="primer3"
                     /Sequence="CTTCAAGATCCGCCACAACATCG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-776; MG-2748"
     primer_bind     1393..1415
                     /%GC="52.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="62.3"
                     /annotation_group="2682260547438004b9edede-d0fd-4139-a976-
                     003d274901a3;
                     2682268566269990c51739a-e9a5-44a4-a64b-1321e27af678"
                     /created_by="primer3"
                     /Sequence="CTTCAAGATCCGCCACAACATCG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-776; MG-2748"
     primer_bind     complement(1398..1417)
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.2"
                     /annotation_group="26741266608100013e31c0d-64a6-4768-86c3-
                     b2d00966b446"
                     /created_by="primer3"
                     /Sequence="CTCGATGTTGTGGCGGATCT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-3141-ES-R"
     primer_bind     complement(1398..1417)
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.2"
                     /annotation_group="268226914367000a3e191c5-0e97-4262-beb3-
                     a54391d74a4a"
                     /created_by="primer3"
                     /Sequence="CTCGATGTTGTGGCGGATCT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-3141-ES-R"
     primer_bind     1401..1420
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="3.1"
                     /Tm="62.5"
                     /annotation_group="26741155675870074c820e8-79b6-4791-a7f2-
                     8abf52b11b86"
                     /created_by="primer3"
                     /Sequence="TCCGCCACAACATCGAGGAC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-1026"
     primer_bind     1401..1420
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="3.1"
                     /Tm="62.5"
                     /annotation_group="268225763938300f1ac59da-bc30-4f61-8c0e-
                     4092b5d42c4a"
                     /created_by="primer3"
                     /Sequence="TCCGCCACAACATCGAGGAC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-1026"
     primer_bind     1429..1448
                     /%GC="70.0"
                     /Hairpin_Tm="32.9"
                     /Self_Dimer_Tm="None"
                     /Tm="65.6"
                     /annotation_group="267411971228300580948ea-3b41-4250-a89d-
                     89c4f2fff2a6"
                     /created_by="primer3"
                     /Sequence="GCAGCTCGCCGACCACTACC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-324"
     primer_bind     1429..1448
                     /%GC="70.0"
                     /Hairpin_Tm="32.9"
                     /Self_Dimer_Tm="None"
                     /Tm="65.6"
                     /annotation_group="26822622525740069f2e1e0-43ed-45fb-8dce-
                     b7b445f6303c"
                     /created_by="primer3"
                     /Sequence="GCAGCTCGCCGACCACTACC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-324"
     primer_bind     complement(1440..1462)
                     /%GC="60.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="66.2"
                     /annotation_group="2674129550655007058dc41-94fc-4246-bb26-
                     f7dc9e4dc535"
                     /created_by="primer3"
                     /Sequence="GGGGGTGTTCTGCTGGTAGTGGT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-737"
     primer_bind     complement(1440..1462)
                     /%GC="60.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="66.2"
                     /annotation_group="268227201736200aaf7f386-f92b-476e-89c8-
                     9bef161a7aab"
                     /created_by="primer3"
                     /Sequence="GGGGGTGTTCTGCTGGTAGTGGT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-737"
     primer_bind     1442..1461
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.3"
                     /annotation_group="26741284245720067b129d7-f33c-485b-a75c-
                     5c19e45501ff"
                     /created_by="primer3"
                     /Sequence="CACTACCAGCAGAACACCCC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-136"
     primer_bind     1442..1461
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.3"
                     /annotation_group="268227087475700f3fefa7b-7755-46fb-ab46-
                     1df0a7a67e58"
                     /created_by="primer3"
                     /Sequence="CACTACCAGCAGAACACCCC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-136"
     primer_bind     1522..1544
                     /%GC="56.5"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="64.0"
                     /annotation_group="267411436407100fc92f5b3-d91d-411e-9566-
                     fede56321fae"
                     /created_by="primer3"
                     /Sequence="GAGCAAAGACCCCAACGAGAAGC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-2412"
     primer_bind     1522..1544
                     /%GC="56.5"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="64.0"
                     /annotation_group="268225645089600c6605147-5d5a-4988-a769-
                     1f67bd82e174"
                     /created_by="primer3"
                     /Sequence="GAGCAAAGACCCCAACGAGAAGC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-2412"
     primer_bind     1524..1543
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.1"
                     /annotation_group="267413989304500b0e426d5-4535-4cab-833d-
                     924bf9f9a6db"
                     /created_by="primer3"
                     /Sequence="GCAAAGACCCCAACGAGAAG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-2243"
     primer_bind     1524..1543
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.1"
                     /annotation_group="268228236933399a31f7016-0edd-4148-b946-
                     f67d1de804b7"
                     /created_by="primer3"
                     /Sequence="GCAAAGACCCCAACGAGAAG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-2243"
     primer_bind     complement(1534..1556)
                     /%GC="56.5"
                     /Hairpin_Tm="41.1"
                     /Self_Dimer_Tm="5.7"
                     /Tm="64.7"
                     /annotation_group="26741243820099918a335b0-9ce8-48b1-8d20-
                     265c456ad293"
                     /created_by="primer3"
                     /Sequence="CCATGTGATCGCGCTTCTCGTTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-170"
     primer_bind     complement(1534..1556)
                     /%GC="56.5"
                     /Hairpin_Tm="41.1"
                     /Self_Dimer_Tm="5.7"
                     /Tm="64.7"
                     /annotation_group="2682266872436000f179363-792a-4ef1-9837-
                     1a3f9f1d687c"
                     /created_by="primer3"
                     /Sequence="CCATGTGATCGCGCTTCTCGTTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-170"
     primer_bind     1537..1556
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="12.1"
                     /Tm="61.8"
                     /annotation_group="2674131295933006b0e11fe-d86f-4b9c-a9e3-
                     2860252ed02e"
                     /created_by="primer3"
                     /Sequence="CGAGAAGCGCGATCACATGG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-326"
     primer_bind     1537..1556
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="12.1"
                     /Tm="61.8"
                     /annotation_group="26822737466029900b3a073-3790-4502-b192-
                     36c042bda29b"
                     /created_by="primer3"
                     /Sequence="CGAGAAGCGCGATCACATGG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-326"
     primer_bind     1546..1568
                     /%GC="56.5"
                     /Hairpin_Tm="44.2"
                     /Self_Dimer_Tm="13.4"
                     /Tm="64.4"
                     /annotation_group="267412147271799645f147c-c487-4b31-af70-
                     9017e37e63fc"
                     /created_by="primer3"
                     /Sequence="CGATCACATGGTCCTGCTGGAGT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-2410"
     primer_bind     1546..1568
                     /%GC="56.5"
                     /Hairpin_Tm="44.2"
                     /Self_Dimer_Tm="13.4"
                     /Tm="64.4"
                     /annotation_group="2682263981515997f1c9153-b8d1-42f7-b8e0-
                     9fa3b10ad0c2"
                     /created_by="primer3"
                     /Sequence="CGATCACATGGTCCTGCTGGAGT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-2410"
     misc_feature    1653..1898
                     /vntifkey=21
                     /locus_tag="WPRE3"
                     /ApEinfo_label="WPRE3"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="WPRE3"
     primer_bind     complement(1712..1731)
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="56.6"
                     /annotation_group="267413876751800df0f669b-8247-4d6d-80b3-
                     703064036014"
                     /created_by="primer3"
                     /Sequence="CCACATAGCGTAAAAGGAGC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-110"
     primer_bind     complement(1712..1731)
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="56.6"
                     /annotation_group="268228123244100c0fee5a7-fbe6-49a7-b728-
                     8a56da26ac5d"
                     /created_by="primer3"
                     /Sequence="CCACATAGCGTAAAAGGAGC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-110"
     primer_bind     complement(1725..1744)
                     /%GC="40.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="54.3"
                     /annotation_group="26741421708039952f59c31-0fb9-4682-aa8d-
                     1a7865294f61"
                     /created_by="primer3"
                     /Sequence="TAAAGCAGCGTATCCACATA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-2396"
     primer_bind     complement(1725..1744)
                     /%GC="40.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="54.3"
                     /annotation_group="268228469072400601863b9-5e7e-4c17-ba3c-
                     ca48737cbb47"
                     /created_by="primer3"
                     /Sequence="TAAAGCAGCGTATCCACATA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-2396"
     polyA_signal    1912..2115
                     /vntifkey=21
                     /locus_tag="BGH-pA"
                     /ApEinfo_label="BGH-pA"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /modified_by="jacqueline.bendrick"
                     /label="bGHpA"
     primer_bind     1920..1939
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="58.3"
                     /annotation_group="267414272616799c4c23654-ead2-4f8e-b89e-
                     9053ecad5863"
                     /created_by="primer3"
                     /Sequence="CCTTCTAGTTGCCAGCCATC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-241"
     primer_bind     1920..1939
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="58.3"
                     /annotation_group="26822852565149949ce2de6-0b33-4bcc-b8a2-
                     85cb67ab4eb6"
                     /created_by="primer3"
                     /Sequence="CCTTCTAGTTGCCAGCCATC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-241"
     primer_bind     1927..1949
                     /%GC="52.2"
                     /Hairpin_Tm="38.7"
                     /Self_Dimer_Tm="None"
                     /Tm="63.7"
                     /annotation_group="267413012870599e58ca855-2c58-40fc-adb5-
                     390e7b9bb3b3;
                     2674133603596002949e7ed-af4c-40f7-9b8d-6cedb4be51fd;
                     26741416083720081a74d07-1058-4216-9c44-7185e88b24bd"
                     /created_by="primer3"
                     /Sequence="GTTGCCAGCCATCTGTTGTTTGC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="scAAV_R-ITR_2; MG-2274; MG-729"
     primer_bind     complement(1927..1949)
                     /%GC="52.2"
                     /Hairpin_Tm="46.6"
                     /Self_Dimer_Tm="8.4"
                     /Tm="63.7"
                     /annotation_group="2674131877977008b3794d1-e75b-42d0-b831-
                     b0acb4ec6f8f"
                     /created_by="primer3"
                     /Sequence="GCAAACAACAGATGGCTGGCAAC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-523"
     primer_bind     1927..1949
                     /%GC="52.2"
                     /Hairpin_Tm="38.7"
                     /Self_Dimer_Tm="None"
                     /Tm="63.7"
                     /annotation_group="26822725786860078ab4fd2-112c-491b-9ee6-
                     8eaaec938b49;
                     268227603505200edb06520-8f9e-409e-a4fa-680651cd2359;
                     268228410174000e6eef936-3117-4426-bf0e-717c22380831"
                     /created_by="primer3"
                     /Sequence="GTTGCCAGCCATCTGTTGTTTGC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="scAAV_R-ITR_2; MG-2274; MG-729"
     primer_bind     complement(1927..1949)
                     /%GC="52.2"
                     /Hairpin_Tm="46.6"
                     /Self_Dimer_Tm="8.4"
                     /Tm="63.7"
                     /annotation_group="268227432841199b57e857f-e712-424b-a54c-
                     8ed640e52bd4"
                     /created_by="primer3"
                     /Sequence="GCAAACAACAGATGGCTGGCAAC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-523"
     primer_bind     1967..1989
                     /%GC="60.9"
                     /Hairpin_Tm="59.5"
                     /Self_Dimer_Tm="None"
                     /Tm="65.1"
                     /annotation_group="267412722872400e05fe536-90c7-4883-aa3c-
                     4808b96cd011"
                     /created_by="primer3"
                     /Sequence="CTTGACCCTGGAAGGTGCCACTC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-887"
     primer_bind     1967..1989
                     /%GC="60.9"
                     /Hairpin_Tm="59.5"
                     /Self_Dimer_Tm="None"
                     /Tm="65.1"
                     /annotation_group="268226971296900c620b8d3-cda2-410f-a354-
                     cf1aa692e207"
                     /created_by="primer3"
                     /Sequence="CTTGACCCTGGAAGGTGCCACTC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-887"
     primer_bind     complement(1977..1999)
                     /%GC="60.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="15.3"
                     /Tm="66.2"
                     /annotation_group="26741124988350069fe70ef-ae51-4502-9522-
                     5a38e5bc8d6a;
                     26741370456980024cd7320-681b-45f2-a474-ac75928abfc0"
                     /created_by="primer3"
                     /Sequence="AGGACAGTGGGAGTGGCACCTTC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-1080; MG-2202"
     primer_bind     complement(1977..1999)
                     /%GC="60.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="15.3"
                     /Tm="66.2"
                     /annotation_group="26822541279420001004aac-b7e1-460d-b5f4-
                     2b788c74c767;
                     2682279475903004bfbf0d5-a4d7-4195-9073-4d0e6d1366dd"
                     /created_by="primer3"
                     /Sequence="AGGACAGTGGGAGTGGCACCTTC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-1080; MG-2202"
     primer_bind     complement(1991..2010)
                     /%GC="35.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="50.0"
                     /annotation_group="26741208920190009ce0483-fa05-4fc5-a158-
                     4b40eb6ce6db"
                     /created_by="primer3"
                     /Sequence="TTTATTAGGAAAGGACAGTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-65"
     primer_bind     complement(1991..2010)
                     /%GC="35.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="50.0"
                     /annotation_group="268226340177799dec06043-766e-4f35-9181-
                     640bf198a070"
                     /created_by="primer3"
                     /Sequence="TTTATTAGGAAAGGACAGTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-65"
     primer          complement(2026..2045)
                     /vntifkey=27
                     /locus_tag="BGH-R"
                     /ApEinfo_label="BGH-R"
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#80ff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BGH-R"
     primer_bind     2076..2095
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.4"
                     /annotation_group="267414104542800b15fa760-bcad-438c-b07e-
                     1508bdd307ab"
                     /created_by="primer3"
                     /Sequence="GACAGCAAGGGGGAGGATTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-321"
     primer_bind     2076..2095
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.4"
                     /annotation_group="26822835333540047e91c64-215f-4be9-9532-
                     642405644933"
                     /created_by="primer3"
                     /Sequence="GACAGCAAGGGGGAGGATTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-321"
     primer_bind     2082..2104
                     /%GC="52.2"
                     /Hairpin_Tm="34.4"
                     /Self_Dimer_Tm="None"
                     /Tm="63.2"
                     /annotation_group="267411912744399c913542d-b8d6-4c96-a641-
                     a2b082cc4965;
                     267412320566600e66f0869-d6b5-4a19-9fae-005b7d688bf1;
                     2674135903643006bda018c-134d-4626-8506-1cd8a7b59390"
                     /created_by="primer3"
                     /Sequence="AAGGGGGAGGATTGGGAAGACAA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-886; MG-2701; scAAV_R-ITR_1"
     primer_bind     2082..2104
                     /%GC="52.2"
                     /Hairpin_Tm="34.4"
                     /Self_Dimer_Tm="None"
                     /Tm="63.2"
                     /annotation_group="268226169537300da9461bc-4231-47e8-be6e-
                     b055cd855309;
                     268226570018000f6fa4a20-74dd-4451-9fac-455f1124635b;
                     268227831740000ee4ed0cc-a4e5-45a3-834d-ef604ba52848"
                     /created_by="primer3"
                     /Sequence="AAGGGGGAGGATTGGGAAGACAA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-886; MG-2701; scAAV_R-ITR_1"
     repeat_region   2144..2284
                     /note="adeno-associated virus (AAV-2) inverted terminal
                     repeat (ITR) region required for genome multiplication"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AAV2 ITR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:2m2-enavet2"">AAV2 ITR</a>"
                     /label="AAV2 ITR"
     misc_feature    2144..2284
                     /vntifkey=21
                     /locus_tag="R-ITR"
                     /ApEinfo_label="R-ITR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="R-ITR"
     rep_origin      2359..2814
                     /note="f1 replication origin derived from bacteriophage
                     f1"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="F1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:2jn-enavesu"">F1</a>"
                     /label="F1"
     primer_bind     complement(2367..2386)
                     /%GC="60.0"
                     /Hairpin_Tm="71.3"
                     /Self_Dimer_Tm="24.5"
                     /Tm="64.4"
                     /annotation_group="2682255868786006a6b12ce-306b-49f3-9563-
                     8d386c1fc9ac;
                     268225880054700337427b4-7798-4d71-b9c6-5edb676fe5e5"
                     /created_by="primer3"
                     /Sequence="GCGCTTAATGCGCCGCTACA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="Npy-IRES2-Flpo\C - MG-290; MG-290"
     rep_origin      2376..2682
                     /vntifkey=33
                     /locus_tag="f1\Origin"
                     /ApEinfo_label="f1\Origin"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /modified_by="jacqueline.bendrick"
                     /label="f1 Origin"
     promoter        3096..3200
                     /note="Promoter for ampicillin resistance gene
                     (b-lactamase)"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="amp"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1jv-enaveop"">amp</a>"
                     /label="amp"
     misc_feature    3201..4058
                     /vntifkey=21
                     /locus_tag="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="AmpR"
     rep_origin      4209..4876
                     /vntifkey=33
                     /locus_tag="pUC\ori"
                     /ApEinfo_label="pUC\ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="pUC\ori"
     primer          4827..4846
                     /created_by="brooke.wynalda"
                     /label="MG_3012"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtggacag ctacttcaga aggcttgcta ttgttagtgt
      181 aacaaaagct tattctttcc agtgccaaat tataccagtt taatgcagtg gattaagtaa
      241 aatcattgca cactcttggt cttctgaaga agacaatcta taaagtcttt aattctcaaa
      301 ataaaattaa ttgccagggc cacaacagaa tgggtccgcc tctcccaacc ctctgagata
      361 catgcctggc catcctgcag cctcactgcc cagaaggatt aatgatcata tatattaaag
      421 agctggcaca cagcctggta gagcactgag agtggtgttt gtgatcatca ttataatgaa
      481 agtgctcccc ttccaggtgc caagctctat cacctaacag tgtgcagaaa gtattaattg
      541 aactcttttc taccttcttt tagaaatgtg ttcagtgtgt gtgtgtgtgt gtgtgtgtgt
      601 gtgtatgcac cttcctgtgc atgccacatg cacatataga ggtcaaagga caacagtcag
      661 gagcctgttc ttgttttcca cctttttgaa gcagtttcct ttgattctgt ttctgagact
      721 gcattccaga ctaacatgct tccactgatt ttcctagctc ttgcctatct ttgaggggct
      781 gggctatttc actgagtttg agctcgggct gggcataaaa gtcagggcag agccatctat
      841 tgcttacatt tgcttctggg atccagatct ttcgaagcta gcgctaccgg tcgccaccat
      901 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
      961 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     1021 caagctgacc ctgaagctga tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     1081 cgtgaccacc ctgggctacg gcgtgcagtg cttcgcccgc taccccgacc acatgaagca
     1141 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     1201 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     1261 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     1321 gctggagtac aactacaaca gccacaacgt ctatatcacc gccgacaagc agaagaacgg
     1381 catcaaggcc aacttcaaga tccgccacaa catcgaggac ggcggcgtgc agctcgccga
     1441 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     1501 cctgagctac cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     1561 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaagt
     1621 cgacggcgcg ccgcggccgc gaattcgata tcataatcaa cctctggatt acaaaatttg
     1681 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     1741 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     1801 taaatcctgg ttagttcttg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     1861 gacaggggct cggctgttgg gcactgacaa ttccgtggct cgagagatct tcgactgtgc
     1921 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     1981 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     2041 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     2101 acaatagcag gcatgagatc tcacgtgcgg accgagcggc cgcaggaacc cctagtgatg
     2161 gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc
     2221 gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg
     2281 caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     2341 cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     2401 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     2461 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     2521 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg
     2581 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     2641 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     2701 gctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     2761 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt
     2821 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     2881 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     2941 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     3001 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     3061 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     3121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     3181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     3241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     3301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     3361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     3421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     3481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     3541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     3601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     3661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     3721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     3781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     3841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     3901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     3961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     4021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     4081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     4141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     4201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     4261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     4321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     4381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     4441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     4501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     4561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     4621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     4681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     4741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     4801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     4861 gctggccttt tgctcacatg t
//
