LOCUS       HCT44_pAAV-eHGT_1186m-minBglobin-SYFP2-WPRE3-bGHpA        5413 bp    DNA     circular UNA 09-JUL-2024
DEFINITION  Restriction Cloning Ligation Product .
ACCESSION   urn.local...gci-b00ahia
VERSION     urn.local...gci-b00ahia
KEYWORDS    .
SOURCE      
  ORGANISM  Mouse.
FEATURES             Location/Qualifiers
     misc_feature    1..130
                     /vntifkey=21
                     /locus_tag="L-ITR"
                     /ApEinfo_label="L-ITR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="L-ITR"
     misc_feature    46^47
                     /Original_Bases="AAAGCCCGGGC"
                     /label="AAAGCCCGGGC"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    complement(126..1355)
                     /Source="referenceToDocument: urn:local:.:27-hwraz6g"
                     /label="& Trimmed m462-RMSYFP2seqR.ab1 (concatenated
                     sequence 2) (reversed)"
                     /note="Geneious type: Concatenated sequence"
     Enhancer        145..1314
                     /created_by="brooke.wynalda"
                     /modified_by="brooke.wynalda"
                     /label="eGT_1186m_Enhancer"
     primer_bind     145..163
                     /Sequence="CCCTAGTGTTCCAGTGTTC"
                     /Tm="54.8"
                     /%GC="52.6"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /created_by="brooke.wynalda"
                     /label="GT_121"
     primer_bind     complement(1294..1315)
                     /Sequence="CCACAGCAAATCAAAGAAGATG"
                     /Tm="56.2"
                     /%GC="40.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /created_by="brooke.wynalda"
                     /label="GT_122"
     misc_feature    1337..143
                     /Source="referenceToDocument: urn:local:.:26-hwraz6g"
                     /label="CN1244_pAAV Linearized (digested by SacI, MluI)
                     Fragment 3 (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     promoter        1338..1390
                     /vntifkey=21
                     /locus_tag="Beta\Globin\minimal\promoter"
                     /ApEinfo_label="Beta\Globin\minimal\promoter"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /modified_by="jacqueline.bendrick"
                     /label="beta-globin minimal promoter"
     misc_feature    1347..1352
                     /vntifkey=21
                     /locus_tag="TATA\box"
                     /ApEinfo_label="TATA\box"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="TATA\box"
     misc_feature    1379
                     /vntifkey=21
                     /locus_tag="transcriptional\start"
                     /ApEinfo_label="transcriptional\start"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="transcriptional\start"
     primer_bind     1428..1446
                     /%GC="63.2"
                     /Hairpin_Tm="47.5"
                     /Self_Dimer_Tm="19.0"
                     /Tm="62.9"
                     /annotation_group="2674114959566006bfd1120-fbec-4fb0-a4ef-
                     1786f2ea2e82"
                     /created_by="primer3"
                     /Sequence="ACCATGGTGAGCAAGGGCG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-791"
     primer_bind     complement(1428..1446)
                     /%GC="63.2"
                     /Hairpin_Tm="37.1"
                     /Self_Dimer_Tm="13.0"
                     /Tm="62.9"
                     /annotation_group="26741393307789903d436ce-462d-444b-ae29-
                     84b7c64ed2b1"
                     /created_by="primer3"
                     /Sequence="CGCCCTTGCTCACCATGGT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-792"
     primer_bind     1428..1446
                     /%GC="63.2"
                     /Hairpin_Tm="47.5"
                     /Self_Dimer_Tm="19.0"
                     /Tm="62.9"
                     /annotation_group="268225705991100a1c86835-2d9e-4a94-867d-
                     59d0721fdd51"
                     /created_by="primer3"
                     /Sequence="ACCATGGTGAGCAAGGGCG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-791"
     primer_bind     complement(1428..1446)
                     /%GC="63.2"
                     /Hairpin_Tm="37.1"
                     /Self_Dimer_Tm="13.0"
                     /Tm="62.9"
                     /annotation_group="268228180191200bfec062e-823a-4689-a151-
                     95c8c50f0fc4"
                     /created_by="primer3"
                     /Sequence="CGCCCTTGCTCACCATGGT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-792"
     CDS             1431..2150
                     /kind="reporter"
                     /note="Yellow fluorescent reporter protein - Ex:515,
                     Em:527"
                     /Color="#e1ff2f"
                     /product="SYFP2"
                     /gene="SYFP2"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SYFP2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:dx-enavel9"">SYFP2</a>"
                     /label="SYFP2"
     primer_bind     complement(1431..1451)
                     /%GC="61.9"
                     /Hairpin_Tm="34.4"
                     /Self_Dimer_Tm="None"
                     /Tm="64.5"
                     /annotation_group="267411313157700ab081476-d455-484b-9f16-
                     f7265b92726c"
                     /created_by="primer3"
                     /Sequence="CTCCTCGCCCTTGCTCACCAT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-2595"
     primer_bind     complement(1431..1451)
                     /%GC="61.9"
                     /Hairpin_Tm="34.4"
                     /Self_Dimer_Tm="None"
                     /Tm="64.5"
                     /annotation_group="268225471053600fb284203-12a5-4b6f-aa11-
                     a50fa338630d"
                     /created_by="primer3"
                     /Sequence="CTCCTCGCCCTTGCTCACCAT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-2595"
     primer_bind     1440..1459
                     /%GC="60.0"
                     /Hairpin_Tm="47.3"
                     /Self_Dimer_Tm="None"
                     /Tm="62.5"
                     /annotation_group="2674137644551000debb238-62ff-48b0-84db-
                     2273d95aad00"
                     /created_by="primer3"
                     /Sequence="AAGGGCGAGGAGCTGTTCAC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-667"
     primer_bind     1440..1459
                     /%GC="60.0"
                     /Hairpin_Tm="47.3"
                     /Self_Dimer_Tm="None"
                     /Tm="62.5"
                     /annotation_group="268228007567900bf107f4b-dace-47cf-9678-
                     9d0ce8c6c327"
                     /created_by="primer3"
                     /Sequence="AAGGGCGAGGAGCTGTTCAC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-667"
     primer_bind     1475..1494
                     /%GC="75.0"
                     /Hairpin_Tm="37.8"
                     /Self_Dimer_Tm="30.2"
                     /Tm="67.6"
                     /annotation_group="267412029042400bd640d40-19ee-4d12-9e22-
                     213c87aeb9c3"
                     /created_by="primer3"
                     /Sequence="CCTGGTCGAGCTGGACGGCG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-331"
     primer_bind     1475..1494
                     /%GC="75.0"
                     /Hairpin_Tm="37.8"
                     /Self_Dimer_Tm="30.2"
                     /Tm="67.6"
                     /annotation_group="268226282281000335e9e10-e95a-4203-8c38-
                     08482524df12"
                     /created_by="primer3"
                     /Sequence="CCTGGTCGAGCTGGACGGCG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-331"
     primer_bind     1493..1515
                     /%GC="52.2"
                     /Hairpin_Tm="46.5"
                     /Self_Dimer_Tm="2.2"
                     /Tm="63.9"
                     /annotation_group="2674124959641004c3bdab9-67bb-4058-8635-
                     cfc5da5dc9be"
                     /created_by="primer3"
                     /Sequence="CGACGTAAACGGCCACAAGTTCA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-736"
     primer_bind     1493..1515
                     /%GC="52.2"
                     /Hairpin_Tm="46.5"
                     /Self_Dimer_Tm="2.2"
                     /Tm="63.9"
                     /annotation_group="26822674369520051e099fe-a601-405e-9758-
                     e618b8fdca09"
                     /created_by="primer3"
                     /Sequence="CGACGTAAACGGCCACAAGTTCA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-736"
     primer_bind     1493..1512
                     /%GC="55.0"
                     /Hairpin_Tm="46.5"
                     /Self_Dimer_Tm="2.2"
                     /Tm="61.2"
                     /annotation_group="267414048146100c47d653d-e673-4064-a014-
                     182b5cb2d872"
                     /created_by="primer3"
                     /Sequence="CGACGTAAACGGCCACAAGT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-2645"
     primer_bind     1493..1512
                     /%GC="55.0"
                     /Hairpin_Tm="46.5"
                     /Self_Dimer_Tm="2.2"
                     /Tm="61.2"
                     /annotation_group="268228293463600a2c515e3-a96e-4e46-b3ef-
                     e4b6ec799334"
                     /created_by="primer3"
                     /Sequence="CGACGTAAACGGCCACAAGT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-2645"
     primer_bind     complement(1498..1520)
                     /%GC="52.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="64.2"
                     /annotation_group="2674125527081006f937811-e03e-437a-83f0-
                     1772fa76fe10"
                     /created_by="primer3"
                     /Sequence="CACGCTGAACTTGTGGCCGTTTA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-2462"
     primer_bind     complement(1498..1520)
                     /%GC="52.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="64.2"
                     /annotation_group="26822679981429913c8b7a9-10b5-4fff-acc5-
                     f73c489d02cd"
                     /created_by="primer3"
                     /Sequence="CACGCTGAACTTGTGGCCGTTTA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-2462"
     primer_bind     1499..1518
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="2.2"
                     /Tm="62.1"
                     /annotation_group="2674122629687991b662f0d-b2ff-4b6f-a40b-
                     2c716e0ece3f"
                     /created_by="primer3"
                     /Sequence="AAACGGCCACAAGTTCAGCG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-1027"
     primer_bind     1499..1518
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="2.2"
                     /Tm="62.1"
                     /annotation_group="268226512923799f4a38d0a-b6eb-4653-9e50-
                     42272b630244"
                     /created_by="primer3"
                     /Sequence="AAACGGCCACAAGTTCAGCG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-1027"
     primer_bind     complement(1544..1567)
                     /%GC="58.3"
                     /Hairpin_Tm="58.5"
                     /Self_Dimer_Tm="None"
                     /Tm="66.7"
                     /annotation_group="2674123779128000a63cd81-84ab-46e1-ac7e-
                     3e592aa419bb"
                     /created_by="primer3"
                     /Sequence="TTCAGGGTCAGCTTGCCGTAGGTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-598"
     primer_bind     complement(1544..1567)
                     /%GC="58.3"
                     /Hairpin_Tm="58.5"
                     /Self_Dimer_Tm="None"
                     /Tm="66.7"
                     /annotation_group="2682266283519002c557c85-ad28-417d-b2e6-
                     b28f9797a5e7"
                     /created_by="primer3"
                     /Sequence="TTCAGGGTCAGCTTGCCGTAGGTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-598"
     primer_bind     complement(1545..1564)
                     /%GC="60.0"
                     /Hairpin_Tm="58.5"
                     /Self_Dimer_Tm="None"
                     /Tm="63.4"
                     /annotation_group="26741347531369925c9f147-2bed-450a-b081-
                     a5dae9644517;
                     26741353304070092ae149d-f2a5-4953-8fea-69cc724c05e2"
                     /created_by="primer3"
                     /Sequence="AGGGTCAGCTTGCCGTAGGT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-977; MG-160"
     primer_bind     complement(1545..1564)
                     /%GC="60.0"
                     /Hairpin_Tm="58.5"
                     /Self_Dimer_Tm="None"
                     /Tm="63.4"
                     /annotation_group="26822771877980052ff1eaa-1d5d-46da-bfdb-
                     0fefead0e928;
                     2682277748689004255a9d0-f587-47e5-8056-5fc97ef247ab"
                     /created_by="primer3"
                     /Sequence="AGGGTCAGCTTGCCGTAGGT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-977; MG-160"
     primer_bind     1572..1591
                     /%GC="60.0"
                     /Hairpin_Tm="41.4"
                     /Self_Dimer_Tm="None"
                     /Tm="64.9"
                     /annotation_group="26741278488210043a619de-baac-4f09-82de-
                     9299b2274c97"
                     /created_by="primer3"
                     /Sequence="ATCTGCACCACCGGCAAGCT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-135"
     primer_bind     1572..1591
                     /%GC="60.0"
                     /Hairpin_Tm="41.4"
                     /Self_Dimer_Tm="None"
                     /Tm="64.9"
                     /annotation_group="2682270314221993df7db86-a02a-4d68-92d8-
                     56b071fde0f6"
                     /created_by="primer3"
                     /Sequence="ATCTGCACCACCGGCAAGCT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-135"
     primer_bind     1643..1662
                     /%GC="75.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="67.4"
                     /annotation_group="26741167619720041cc659a-68f5-466d-949f-
                     406d63ae464f;
                     267414329676900b9c185a4-3605-4637-a320-93005b784649"
                     /created_by="primer3"
                     /Sequence="CTTCGCCCGCTACCCCGACC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-548; MG-2641"
     primer_bind     1643..1662
                     /%GC="75.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="67.4"
                     /annotation_group="268225937281800eeef4b57-dc1e-4d30-b300-
                     5040c8295f1a;
                     26822858318680039ab94f4-7e3e-4446-b7b3-97b26f94d73c"
                     /created_by="primer3"
                     /Sequence="CTTCGCCCGCTACCCCGACC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-548; MG-2641"
     primer_bind     complement(1652..1671)
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.5"
                     /annotation_group="2674128985969005a169f75-75c7-4008-95eb-
                     179e70fa2507"
                     /created_by="primer3"
                     /Sequence="GCTTCATGTGGTCGGGGTAG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-3143-ES-R"
     primer_bind     complement(1652..1671)
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.5"
                     /annotation_group="268227144880099862bdb6a-5e90-450b-8719-
                     743f8ac05ea8"
                     /created_by="primer3"
                     /Sequence="GCTTCATGTGGTCGGGGTAG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-3143-ES-R"
     primer_bind     1661..1682
                     /%GC="54.5"
                     /Hairpin_Tm="42.2"
                     /Self_Dimer_Tm="None"
                     /Tm="62.3"
                     /annotation_group="2674136475308003a9a5f3e-4337-4fb4-9d58-
                     2199b166c803"
                     /created_by="primer3"
                     /Sequence="CCACATGAAGCAGCACGACTTC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-2836-MM-F"
     primer_bind     1661..1682
                     /%GC="54.5"
                     /Hairpin_Tm="42.2"
                     /Self_Dimer_Tm="None"
                     /Tm="62.3"
                     /annotation_group="2682278891931990ee60755-286b-4400-a608-
                     49d44f437a6f"
                     /created_by="primer3"
                     /Sequence="CCACATGAAGCAGCACGACTTC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-2836-MM-F"
     primer_bind     1662..1681
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.1"
                     /annotation_group="267411738546499b3885738-5cf7-45f5-b70f-
                     a0e6037f3066"
                     /created_by="primer3"
                     /Sequence="CACATGAAGCAGCACGACTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-129"
     primer_bind     1662..1681
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.1"
                     /annotation_group="2682259951647005c2624f4-3104-49ab-81d7-
                     b3ff611ea54a"
                     /created_by="primer3"
                     /Sequence="CACATGAAGCAGCACGACTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-129"
     primer_bind     complement(1706..1729)
                     /%GC="58.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="27.2"
                     /Tm="66.5"
                     /annotation_group="26741382075440072520115-a4f7-4215-a874-
                     3d463d9bf95b"
                     /created_by="primer3"
                     /Sequence="AAGATGGTGCGCTCCTGGACGTAG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-158"
     primer_bind     complement(1706..1729)
                     /%GC="58.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="27.2"
                     /Tm="66.5"
                     /annotation_group="2682280652442004a9ec3cb-860b-40ae-b470-
                     1cce0714b1a3"
                     /created_by="primer3"
                     /Sequence="AAGATGGTGCGCTCCTGGACGTAG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-158"
     primer_bind     1712..1729
                     /%GC="61.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="26.0"
                     /Tm="59.7"
                     /annotation_group="2674144440330999cf47a6a-d778-4bc0-864e-
                     5dcddc7ac10d"
                     /created_by="primer3"
                     /Sequence="CCAGGAGCGCACCATCTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-111"
     primer_bind     1712..1729
                     /%GC="61.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="26.0"
                     /Tm="59.7"
                     /annotation_group="2682287001684009447d0f6-b139-4dd5-9a48-
                     673a5138554a"
                     /created_by="primer3"
                     /Sequence="CCAGGAGCGCACCATCTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-111"
     primer_bind     complement(1713..1735)
                     /%GC="52.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="27.2"
                     /Tm="63.3"
                     /annotation_group="2674113730263007bb01a25-0cf9-4884-bf4e-
                     2f6763f2e498;
                     2674132447892004aa5b78d-4b12-435b-8296-030ef25cd804"
                     /created_by="primer3"
                     /Sequence="TTGAAGAAGATGGTGCGCTCCTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-777; MG-2467"
     primer_bind     complement(1713..1735)
                     /%GC="52.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="27.2"
                     /Tm="63.3"
                     /annotation_group="2682255293740991c69ecfe-e739-4ec2-8d99-
                     f68330a4abfd;
                     268227489622999d3e6423a-7c81-4fab-8918-133accaf46be"
                     /created_by="primer3"
                     /Sequence="TTGAAGAAGATGGTGCGCTCCTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-777; MG-2467"
     primer_bind     1729..1748
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="1.6"
                     /Tm="60.6"
                     /annotation_group="2674133020390993b496e9d-a602-4a72-9d4b-
                     54ae379a5fc2"
                     /created_by="primer3"
                     /Sequence="TCTTCAAGGACGACGGCAAC"
                     /Mismatches=0
                     /#_Off-target_Sites=1
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA<br>"
                     /Off-target_#1="<html><font
                     color=""red""><b>A</b></font>CTTCAAGGA<font
                     color=""red""><b>G</b></font>GACGGCAAC</html>"
                     /Location_#1="<html>1,339 -&gt; 1,358 (AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA)</html>"
                     /label="MG-2709"
     primer_bind     1729..1748
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="1.6"
                     /Tm="60.6"
                     /annotation_group="268227546899900707ec0b3-fe17-47a1-ac82-
                     dcc31350e42c"
                     /created_by="primer3"
                     /Sequence="TCTTCAAGGACGACGGCAAC"
                     /Mismatches=0
                     /#_Off-target_Sites=1
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA<br>"
                     /Off-target_#1="<html><font
                     color=""red""><b>A</b></font>CTTCAAGGA<font
                     color=""red""><b>G</b></font>GACGGCAAC</html>"
                     /Location_#1="<html>1,339 -&gt; 1,358 (AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA)</html>"
                     /label="MG-2709"
     primer_bind     1746..1765
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="64.3"
                     /annotation_group="26741161711820049095d55-cfe9-47c3-b28b-
                     867056dfbd2a"
                     /created_by="primer3"
                     /Sequence="AACTACAAGACCCGCGCCGA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-627"
     primer_bind     1746..1765
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="64.3"
                     /annotation_group="268225822236299ede3a2d2-7b40-457a-b132-
                     a58a31178ac0"
                     /created_by="primer3"
                     /Sequence="AACTACAAGACCCGCGCCGA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-627"
     primer_bind     complement(1764..1783)
                     /%GC="60.0"
                     /Hairpin_Tm="42.4"
                     /Self_Dimer_Tm="None"
                     /Tm="61.9"
                     /annotation_group="2674134183220000d7df526-d7f2-431e-902b-
                     bf945b0500fe"
                     /created_by="primer3"
                     /Sequence="TCGCCCTCGAACTTCACCTC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-62"
     primer_bind     complement(1764..1783)
                     /%GC="60.0"
                     /Hairpin_Tm="42.4"
                     /Self_Dimer_Tm="None"
                     /Tm="61.9"
                     /annotation_group="268227662973599eb4edfb3-2f79-4399-a647-
                     c36fdb05d642"
                     /created_by="primer3"
                     /Sequence="TCGCCCTCGAACTTCACCTC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-62"
     primer_bind     1817..1836
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="62.2"
                     /annotation_group="2674130725738006e5fc539-a586-425e-9d20-
                     a708e48fbaa4"
                     /created_by="primer3"
                     /Sequence="CGACTTCAAGGAGGACGGCA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-144"
     primer_bind     1817..1836
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="62.2"
                     /annotation_group="26822731525589972790ab7-303b-43c6-bf49-
                     0b1064dc0478"
                     /created_by="primer3"
                     /Sequence="CGACTTCAAGGAGGACGGCA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-144"
     primer_bind     1845..1864
                     /%GC="55.0"
                     /Hairpin_Tm="41.7"
                     /Self_Dimer_Tm="None"
                     /Tm="60.3"
                     /annotation_group="267414387800999594dca57-9329-4ad1-8638-
                     89a44afc8958"
                     /created_by="primer3"
                     /Sequence="GGGCACAAGCTGGAGTACAA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-3140-ES-F"
     primer_bind     1845..1864
                     /%GC="55.0"
                     /Hairpin_Tm="41.7"
                     /Self_Dimer_Tm="None"
                     /Tm="60.3"
                     /annotation_group="26822864026620046a6d84c-d712-456a-81fc-
                     f011d2d94421"
                     /created_by="primer3"
                     /Sequence="GGGCACAAGCTGGAGTACAA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-3140-ES-F"
     primer_bind     1859..1878
                     /%GC="45.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="55.6"
                     /annotation_group="267411854696300d78eb1c8-8e39-4cad-a32f-
                     c93910a9f3fe"
                     /created_by="primer3"
                     /Sequence="GTACAACTACAACAGCCACA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-668"
     primer_bind     1859..1878
                     /%GC="45.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="55.6"
                     /annotation_group="268226111931500efa4420b-5557-45e1-b25d-
                     44c3d2858256"
                     /created_by="primer3"
                     /Sequence="GTACAACTACAACAGCCACA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-668"
     primer_bind     1876..1895
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="57.5"
                     /annotation_group="267412204882500787da284-21cb-489f-8d7b-
                     0b85eac53fa1"
                     /created_by="primer3"
                     /Sequence="ACAACGTCTATATCACCGCC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-332"
     primer_bind     1876..1895
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="57.5"
                     /annotation_group="2682264547963999dd023bd-9bce-4c3f-81b4-
                     da593d987cec"
                     /created_by="primer3"
                     /Sequence="ACAACGTCTATATCACCGCC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-332"
     primer_bind     1925..1947
                     /%GC="52.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="62.3"
                     /annotation_group="267411796101100c06fe419-583b-411b-856c-
                     860c017d311f;
                     26741260922340067be3c63-c47d-47e6-b838-fab263ec1655"
                     /created_by="primer3"
                     /Sequence="CTTCAAGATCCGCCACAACATCG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-776; MG-2748"
     primer_bind     1925..1947
                     /%GC="52.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="62.3"
                     /annotation_group="2682260547438004b9edede-d0fd-4139-a976-
                     003d274901a3;
                     2682268566269990c51739a-e9a5-44a4-a64b-1321e27af678"
                     /created_by="primer3"
                     /Sequence="CTTCAAGATCCGCCACAACATCG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-776; MG-2748"
     primer_bind     complement(1930..1949)
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.2"
                     /annotation_group="26741266608100013e31c0d-64a6-4768-86c3-
                     b2d00966b446"
                     /created_by="primer3"
                     /Sequence="CTCGATGTTGTGGCGGATCT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-3141-ES-R"
     primer_bind     complement(1930..1949)
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.2"
                     /annotation_group="268226914367000a3e191c5-0e97-4262-beb3-
                     a54391d74a4a"
                     /created_by="primer3"
                     /Sequence="CTCGATGTTGTGGCGGATCT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-3141-ES-R"
     primer_bind     1933..1952
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="3.1"
                     /Tm="62.5"
                     /annotation_group="26741155675870074c820e8-79b6-4791-a7f2-
                     8abf52b11b86"
                     /created_by="primer3"
                     /Sequence="TCCGCCACAACATCGAGGAC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-1026"
     primer_bind     1933..1952
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="3.1"
                     /Tm="62.5"
                     /annotation_group="268225763938300f1ac59da-bc30-4f61-8c0e-
                     4092b5d42c4a"
                     /created_by="primer3"
                     /Sequence="TCCGCCACAACATCGAGGAC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-1026"
     primer_bind     1961..1980
                     /%GC="70.0"
                     /Hairpin_Tm="32.9"
                     /Self_Dimer_Tm="None"
                     /Tm="65.6"
                     /annotation_group="267411971228300580948ea-3b41-4250-a89d-
                     89c4f2fff2a6"
                     /created_by="primer3"
                     /Sequence="GCAGCTCGCCGACCACTACC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-324"
     primer_bind     1961..1980
                     /%GC="70.0"
                     /Hairpin_Tm="32.9"
                     /Self_Dimer_Tm="None"
                     /Tm="65.6"
                     /annotation_group="26822622525740069f2e1e0-43ed-45fb-8dce-
                     b7b445f6303c"
                     /created_by="primer3"
                     /Sequence="GCAGCTCGCCGACCACTACC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-324"
     primer_bind     complement(1972..1994)
                     /%GC="60.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="66.2"
                     /annotation_group="2674129550655007058dc41-94fc-4246-bb26-
                     f7dc9e4dc535"
                     /created_by="primer3"
                     /Sequence="GGGGGTGTTCTGCTGGTAGTGGT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-737"
     primer_bind     complement(1972..1994)
                     /%GC="60.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="66.2"
                     /annotation_group="268227201736200aaf7f386-f92b-476e-89c8-
                     9bef161a7aab"
                     /created_by="primer3"
                     /Sequence="GGGGGTGTTCTGCTGGTAGTGGT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-737"
     primer_bind     1974..1993
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.3"
                     /annotation_group="26741284245720067b129d7-f33c-485b-a75c-
                     5c19e45501ff"
                     /created_by="primer3"
                     /Sequence="CACTACCAGCAGAACACCCC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-136"
     primer_bind     1974..1993
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.3"
                     /annotation_group="268227087475700f3fefa7b-7755-46fb-ab46-
                     1df0a7a67e58"
                     /created_by="primer3"
                     /Sequence="CACTACCAGCAGAACACCCC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-136"
     primer_bind     2054..2076
                     /%GC="56.5"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="64.0"
                     /annotation_group="267411436407100fc92f5b3-d91d-411e-9566-
                     fede56321fae"
                     /created_by="primer3"
                     /Sequence="GAGCAAAGACCCCAACGAGAAGC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-2412"
     primer_bind     2054..2076
                     /%GC="56.5"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="64.0"
                     /annotation_group="268225645089600c6605147-5d5a-4988-a769-
                     1f67bd82e174"
                     /created_by="primer3"
                     /Sequence="GAGCAAAGACCCCAACGAGAAGC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-2412"
     primer_bind     2056..2075
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.1"
                     /annotation_group="267413989304500b0e426d5-4535-4cab-833d-
                     924bf9f9a6db"
                     /created_by="primer3"
                     /Sequence="GCAAAGACCCCAACGAGAAG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-2243"
     primer_bind     2056..2075
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.1"
                     /annotation_group="268228236933399a31f7016-0edd-4148-b946-
                     f67d1de804b7"
                     /created_by="primer3"
                     /Sequence="GCAAAGACCCCAACGAGAAG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-2243"
     primer_bind     complement(2066..2088)
                     /%GC="56.5"
                     /Hairpin_Tm="41.1"
                     /Self_Dimer_Tm="5.7"
                     /Tm="64.7"
                     /annotation_group="26741243820099918a335b0-9ce8-48b1-8d20-
                     265c456ad293"
                     /created_by="primer3"
                     /Sequence="CCATGTGATCGCGCTTCTCGTTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-170"
     primer_bind     complement(2066..2088)
                     /%GC="56.5"
                     /Hairpin_Tm="41.1"
                     /Self_Dimer_Tm="5.7"
                     /Tm="64.7"
                     /annotation_group="2682266872436000f179363-792a-4ef1-9837-
                     1a3f9f1d687c"
                     /created_by="primer3"
                     /Sequence="CCATGTGATCGCGCTTCTCGTTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-170"
     primer_bind     2069..2088
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="12.1"
                     /Tm="61.8"
                     /annotation_group="2674131295933006b0e11fe-d86f-4b9c-a9e3-
                     2860252ed02e"
                     /created_by="primer3"
                     /Sequence="CGAGAAGCGCGATCACATGG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-326"
     primer_bind     2069..2088
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="12.1"
                     /Tm="61.8"
                     /annotation_group="26822737466029900b3a073-3790-4502-b192-
                     36c042bda29b"
                     /created_by="primer3"
                     /Sequence="CGAGAAGCGCGATCACATGG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-326"
     primer_bind     2078..2100
                     /%GC="56.5"
                     /Hairpin_Tm="44.2"
                     /Self_Dimer_Tm="13.4"
                     /Tm="64.4"
                     /annotation_group="267412147271799645f147c-c487-4b31-af70-
                     9017e37e63fc"
                     /created_by="primer3"
                     /Sequence="CGATCACATGGTCCTGCTGGAGT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-2410"
     primer_bind     2078..2100
                     /%GC="56.5"
                     /Hairpin_Tm="44.2"
                     /Self_Dimer_Tm="13.4"
                     /Tm="64.4"
                     /annotation_group="2682263981515997f1c9153-b8d1-42f7-b8e0-
                     9fa3b10ad0c2"
                     /created_by="primer3"
                     /Sequence="CGATCACATGGTCCTGCTGGAGT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-2410"
     misc_feature    2185..2430
                     /vntifkey=21
                     /locus_tag="WPRE3"
                     /ApEinfo_label="WPRE3"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="WPRE3"
     primer_bind     complement(2244..2263)
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="56.6"
                     /annotation_group="267413876751800df0f669b-8247-4d6d-80b3-
                     703064036014"
                     /created_by="primer3"
                     /Sequence="CCACATAGCGTAAAAGGAGC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-110"
     primer_bind     complement(2244..2263)
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="56.6"
                     /annotation_group="268228123244100c0fee5a7-fbe6-49a7-b728-
                     8a56da26ac5d"
                     /created_by="primer3"
                     /Sequence="CCACATAGCGTAAAAGGAGC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-110"
     primer_bind     complement(2257..2276)
                     /%GC="40.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="54.3"
                     /annotation_group="26741421708039952f59c31-0fb9-4682-aa8d-
                     1a7865294f61"
                     /created_by="primer3"
                     /Sequence="TAAAGCAGCGTATCCACATA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-2396"
     primer_bind     complement(2257..2276)
                     /%GC="40.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="54.3"
                     /annotation_group="268228469072400601863b9-5e7e-4c17-ba3c-
                     ca48737cbb47"
                     /created_by="primer3"
                     /Sequence="TAAAGCAGCGTATCCACATA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-2396"
     polyA_signal    2444..2647
                     /vntifkey=21
                     /locus_tag="BGH-pA"
                     /ApEinfo_label="BGH-pA"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /modified_by="jacqueline.bendrick"
                     /label="bGHpA"
     primer_bind     2452..2471
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="58.3"
                     /annotation_group="267414272616799c4c23654-ead2-4f8e-b89e-
                     9053ecad5863"
                     /created_by="primer3"
                     /Sequence="CCTTCTAGTTGCCAGCCATC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-241"
     primer_bind     2452..2471
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="58.3"
                     /annotation_group="26822852565149949ce2de6-0b33-4bcc-b8a2-
                     85cb67ab4eb6"
                     /created_by="primer3"
                     /Sequence="CCTTCTAGTTGCCAGCCATC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-241"
     primer_bind     2459..2481
                     /%GC="52.2"
                     /Hairpin_Tm="38.7"
                     /Self_Dimer_Tm="None"
                     /Tm="63.7"
                     /annotation_group="267413012870599e58ca855-2c58-40fc-adb5-
                     390e7b9bb3b3;
                     2674133603596002949e7ed-af4c-40f7-9b8d-6cedb4be51fd;
                     26741416083720081a74d07-1058-4216-9c44-7185e88b24bd"
                     /created_by="primer3"
                     /Sequence="GTTGCCAGCCATCTGTTGTTTGC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="scAAV_R-ITR_2; MG-2274; MG-729"
     primer_bind     complement(2459..2481)
                     /%GC="52.2"
                     /Hairpin_Tm="46.6"
                     /Self_Dimer_Tm="8.4"
                     /Tm="63.7"
                     /annotation_group="2674131877977008b3794d1-e75b-42d0-b831-
                     b0acb4ec6f8f"
                     /created_by="primer3"
                     /Sequence="GCAAACAACAGATGGCTGGCAAC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-523"
     primer_bind     2459..2481
                     /%GC="52.2"
                     /Hairpin_Tm="38.7"
                     /Self_Dimer_Tm="None"
                     /Tm="63.7"
                     /annotation_group="26822725786860078ab4fd2-112c-491b-9ee6-
                     8eaaec938b49;
                     268227603505200edb06520-8f9e-409e-a4fa-680651cd2359;
                     268228410174000e6eef936-3117-4426-bf0e-717c22380831"
                     /created_by="primer3"
                     /Sequence="GTTGCCAGCCATCTGTTGTTTGC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="scAAV_R-ITR_2; MG-2274; MG-729"
     primer_bind     complement(2459..2481)
                     /%GC="52.2"
                     /Hairpin_Tm="46.6"
                     /Self_Dimer_Tm="8.4"
                     /Tm="63.7"
                     /annotation_group="268227432841199b57e857f-e712-424b-a54c-
                     8ed640e52bd4"
                     /created_by="primer3"
                     /Sequence="GCAAACAACAGATGGCTGGCAAC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-523"
     primer_bind     2499..2521
                     /%GC="60.9"
                     /Hairpin_Tm="59.5"
                     /Self_Dimer_Tm="None"
                     /Tm="65.1"
                     /annotation_group="267412722872400e05fe536-90c7-4883-aa3c-
                     4808b96cd011"
                     /created_by="primer3"
                     /Sequence="CTTGACCCTGGAAGGTGCCACTC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-887"
     primer_bind     2499..2521
                     /%GC="60.9"
                     /Hairpin_Tm="59.5"
                     /Self_Dimer_Tm="None"
                     /Tm="65.1"
                     /annotation_group="268226971296900c620b8d3-cda2-410f-a354-
                     cf1aa692e207"
                     /created_by="primer3"
                     /Sequence="CTTGACCCTGGAAGGTGCCACTC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-887"
     primer_bind     complement(2509..2531)
                     /%GC="60.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="15.3"
                     /Tm="66.2"
                     /annotation_group="26741124988350069fe70ef-ae51-4502-9522-
                     5a38e5bc8d6a;
                     26741370456980024cd7320-681b-45f2-a474-ac75928abfc0"
                     /created_by="primer3"
                     /Sequence="AGGACAGTGGGAGTGGCACCTTC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-1080; MG-2202"
     primer_bind     complement(2509..2531)
                     /%GC="60.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="15.3"
                     /Tm="66.2"
                     /annotation_group="26822541279420001004aac-b7e1-460d-b5f4-
                     2b788c74c767;
                     2682279475903004bfbf0d5-a4d7-4195-9073-4d0e6d1366dd"
                     /created_by="primer3"
                     /Sequence="AGGACAGTGGGAGTGGCACCTTC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-1080; MG-2202"
     primer_bind     complement(2523..2542)
                     /%GC="35.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="50.0"
                     /annotation_group="26741208920190009ce0483-fa05-4fc5-a158-
                     4b40eb6ce6db"
                     /created_by="primer3"
                     /Sequence="TTTATTAGGAAAGGACAGTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-65"
     primer_bind     complement(2523..2542)
                     /%GC="35.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="50.0"
                     /annotation_group="268226340177799dec06043-766e-4f35-9181-
                     640bf198a070"
                     /created_by="primer3"
                     /Sequence="TTTATTAGGAAAGGACAGTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-65"
     primer          complement(2558..2577)
                     /vntifkey=27
                     /locus_tag="BGH-R"
                     /ApEinfo_label="BGH-R"
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#80ff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BGH-R"
     primer_bind     2608..2627
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.4"
                     /annotation_group="267414104542800b15fa760-bcad-438c-b07e-
                     1508bdd307ab"
                     /created_by="primer3"
                     /Sequence="GACAGCAAGGGGGAGGATTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-321"
     primer_bind     2608..2627
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.4"
                     /annotation_group="26822835333540047e91c64-215f-4be9-9532-
                     642405644933"
                     /created_by="primer3"
                     /Sequence="GACAGCAAGGGGGAGGATTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-321"
     primer_bind     2614..2636
                     /%GC="52.2"
                     /Hairpin_Tm="34.4"
                     /Self_Dimer_Tm="None"
                     /Tm="63.2"
                     /annotation_group="267411912744399c913542d-b8d6-4c96-a641-
                     a2b082cc4965;
                     267412320566600e66f0869-d6b5-4a19-9fae-005b7d688bf1;
                     2674135903643006bda018c-134d-4626-8506-1cd8a7b59390"
                     /created_by="primer3"
                     /Sequence="AAGGGGGAGGATTGGGAAGACAA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-886; MG-2701; scAAV_R-ITR_1"
     primer_bind     2614..2636
                     /%GC="52.2"
                     /Hairpin_Tm="34.4"
                     /Self_Dimer_Tm="None"
                     /Tm="63.2"
                     /annotation_group="268226169537300da9461bc-4231-47e8-be6e-
                     b055cd855309;
                     268226570018000f6fa4a20-74dd-4451-9fac-455f1124635b;
                     268227831740000ee4ed0cc-a4e5-45a3-834d-ef604ba52848"
                     /created_by="primer3"
                     /Sequence="AAGGGGGAGGATTGGGAAGACAA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-886; MG-2701; scAAV_R-ITR_1"
     repeat_region   2676..2816
                     /note="adeno-associated virus (AAV-2) inverted terminal
                     repeat (ITR) region required for genome multiplication"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AAV2 ITR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:2m2-enavet2"">AAV2 ITR</a>"
                     /label="AAV2 ITR"
     misc_feature    2676..2816
                     /vntifkey=21
                     /locus_tag="R-ITR"
                     /ApEinfo_label="R-ITR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="R-ITR"
     rep_origin      2891..3346
                     /note="f1 replication origin derived from bacteriophage
                     f1"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="F1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:2jn-enavesu"">F1</a>"
                     /label="F1"
     primer_bind     complement(2899..2918)
                     /%GC="60.0"
                     /Hairpin_Tm="71.3"
                     /Self_Dimer_Tm="24.5"
                     /Tm="64.4"
                     /annotation_group="2682255868786006a6b12ce-306b-49f3-9563-
                     8d386c1fc9ac;
                     268225880054700337427b4-7798-4d71-b9c6-5edb676fe5e5"
                     /created_by="primer3"
                     /Sequence="GCGCTTAATGCGCCGCTACA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="Npy-IRES2-Flpo\C - MG-290; MG-290"
     rep_origin      2908..3214
                     /vntifkey=33
                     /locus_tag="f1\Origin"
                     /ApEinfo_label="f1\Origin"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /modified_by="jacqueline.bendrick"
                     /label="f1 Origin"
     promoter        3628..3732
                     /note="Promoter for ampicillin resistance gene
                     (b-lactamase)"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="amp"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1jv-enaveop"">amp</a>"
                     /label="amp"
     misc_feature    3733..4590
                     /vntifkey=21
                     /locus_tag="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="AmpR"
     rep_origin      4741..5408
                     /vntifkey=33
                     /locus_tag="pUC\ori"
                     /ApEinfo_label="pUC\ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="pUC\ori"
     primer          5359..5378
                     /created_by="brooke.wynalda"
                     /label="MG_3012"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtccctag tgttccagtg ttcccatggc aggggaacac
      181 tcactctaat tccagccctg aattctaact taagaaccaa ggtcaccctt gatggtggca
      241 acttcactac cttagtattc tctctctctc tctctctctc tctctctctc tctctctctc
      301 tctctctctc catttatttt atgcatatga gtacactgtt gctgtcttca aacacaccag
      361 aagagggcat cagatcccat taaagatggt tgtaagccac catgtggtgg gtgctgggaa
      421 ttgaactcag gacctcttaa ccactgaacc atctctccag cccctacctc agtattttca
      481 gaccagtgtt gggcagattt aatttccaga cccctcctca ctcggggcct ggtttgtggt
      541 tttgtctaag tgatgacatc acttcggtgt cttcagagag catcctccac tgtcaggaga
      601 tacgcccact cagccaccag ccattgctgc acactgcatc atgtacacat actggagatg
      661 atctgaaagc gggtacccta gaggccgctc ctccgttccc caggtggggc tttagccagg
      721 tatcagtgga gctgggagca gacgtcttct ggacatccag agttgagcat gggaaccttc
      781 caacagagcc acctgctgca gcctgacaga ctgacgtaag gaaggtggaa acactctgtc
      841 cctggccttg gctgaccagc agaagaggat tgagaaatgt gtctggcact aaggatgctg
      901 gagaatgcat aagtagccag ctcacttact ggaagcaaaa tgatctaatc tgcctagcca
      961 gcctgaatct taatgggaag accacgccgc aagggtggga aggatggaaa gagggcttct
     1021 ctgtgcctgg agaggaagct tggctatgac caactcctgt ggccatctgc cattcgtcct
     1081 gcctgggaaa cgcagtggtc ctttcaaaac agtggtgctg ctgaaaatgt caccttgtaa
     1141 cccaagagct acctggtggg actaaaatgc acatggacaa ggagcaagat ttttaagtga
     1201 gcacctgagt gcgcctggcc ctctcatgac tgctgtcttc tgccaagaag gttttgatga
     1261 acaaatccat ctatgtattt ttgcagaaaa tatcatcttc tttgatttgc tgtggctatt
     1321 tcactgagtt tgagctcggg ctgggcataa aagtcagggc agagccatct attgcttaca
     1381 tttgcttctg ggatccagat ctttcgaagc tagcgctacc ggtcgccacc atggtgagca
     1441 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     1501 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     1561 ccctgaagct gatctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     1621 ccctgggcta cggcgtgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact
     1681 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     1741 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     1801 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     1861 acaactacaa cagccacaac gtctatatca ccgccgacaa gcagaagaac ggcatcaagg
     1921 ccaacttcaa gatccgccac aacatcgagg acggcggcgt gcagctcgcc gaccactacc
     1981 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct
     2041 accagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     2101 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa gtcgacggcg
     2161 cgccgcggcc gcgaattcga tatcataatc aacctctgga ttacaaaatt tgtgaaagat
     2221 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     2281 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     2341 ggttagttct tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     2401 ctcggctgtt gggcactgac aattccgtgg ctcgagagat cttcgactgt gccttctagt
     2461 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     2521 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     2581 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     2641 aggcatgaga tctcacgtgc ggaccgagcg gccgcaggaa cccctagtga tggagttggc
     2701 cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg
     2761 cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg
     2821 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag
     2881 caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     2941 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     3001 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     3061 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca
     3121 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     3181 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc gggctattct
     3241 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     3301 caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc
     3361 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     3421 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     3481 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
     3541 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     3601 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     3661 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     3721 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     3781 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     3841 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     3901 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     3961 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     4021 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     4081 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     4141 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     4201 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     4261 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     4321 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     4381 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     4441 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     4501 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     4561 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     4621 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     4681 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     4741 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     4801 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     4861 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     4921 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     4981 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     5041 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     5101 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     5161 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     5221 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     5281 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     5341 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     5401 tttgctcaca tgt
//
