LOCUS       HCT45_pAAV-eHGT_1187m-minBglobin-SYFP2-WPRE3-bGHpA        5168 bp    DNA     circular UNA 09-JUL-2024
DEFINITION  Restriction Cloning Ligation Product .
ACCESSION   urn.local...gci-b00ahia
VERSION     urn.local...gci-b00ahia
KEYWORDS    .
SOURCE      
  ORGANISM  Mouse.
FEATURES             Location/Qualifiers
     misc_feature    1..130
                     /vntifkey=21
                     /locus_tag="L-ITR"
                     /ApEinfo_label="L-ITR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="L-ITR"
     misc_feature    46^47
                     /Original_Bases="AAAGCCCGGGC"
                     /label="AAAGCCCGGGC"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    complement(126..1110)
                     /Source="referenceToDocument: urn:local:.:2f-hwraz6j"
                     /label="& Trimmed m462-RMSYFP2seqR.ab1 (concatenated
                     sequence 2) (reversed)"
                     /note="Geneious type: Concatenated sequence"
     Enhancer        145..1069
                     /created_by="brooke.wynalda"
                     /label="eGT_1187m_Enhancer"
     primer_bind     145..163
                     /created_by="brooke.wynalda"
                     /Sequence="GCTATTGTTCCTGGCTGTG"
                     /Tm="56.3"
                     /%GC="52.6"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /modified_by="brooke.wynalda"
                     /label="GT_123"
     primer_bind     complement(1051..1069)
                     /created_by="brooke.wynalda"
                     /Sequence="GTTACTGGCAGCCAAACTC"
                     /Tm="56.5"
                     /%GC="52.6"
                     /Hairpin_Tm="30.8"
                     /Self_Dimer_Tm="17.0"
                     /modified_by="brooke.wynalda"
                     /label="GT_124"
     misc_feature    1092..143
                     /Source="referenceToDocument: urn:local:.:2e-hwraz6j"
                     /label="CN1244_pAAV Linearized (digested by SacI, MluI)
                     Fragment 3 (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     promoter        1093..1145
                     /vntifkey=21
                     /locus_tag="Beta\Globin\minimal\promoter"
                     /ApEinfo_label="Beta\Globin\minimal\promoter"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /modified_by="jacqueline.bendrick"
                     /label="beta-globin minimal promoter"
     misc_feature    1102..1107
                     /vntifkey=21
                     /locus_tag="TATA\box"
                     /ApEinfo_label="TATA\box"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="TATA\box"
     misc_feature    1134
                     /vntifkey=21
                     /locus_tag="transcriptional\start"
                     /ApEinfo_label="transcriptional\start"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="transcriptional\start"
     primer_bind     1183..1201
                     /%GC="63.2"
                     /Hairpin_Tm="47.5"
                     /Self_Dimer_Tm="19.0"
                     /Tm="62.9"
                     /annotation_group="2674114959566006bfd1120-fbec-4fb0-a4ef-
                     1786f2ea2e82"
                     /created_by="primer3"
                     /Sequence="ACCATGGTGAGCAAGGGCG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-791"
     primer_bind     complement(1183..1201)
                     /%GC="63.2"
                     /Hairpin_Tm="37.1"
                     /Self_Dimer_Tm="13.0"
                     /Tm="62.9"
                     /annotation_group="26741393307789903d436ce-462d-444b-ae29-
                     84b7c64ed2b1"
                     /created_by="primer3"
                     /Sequence="CGCCCTTGCTCACCATGGT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-792"
     primer_bind     1183..1201
                     /%GC="63.2"
                     /Hairpin_Tm="47.5"
                     /Self_Dimer_Tm="19.0"
                     /Tm="62.9"
                     /annotation_group="268225705991100a1c86835-2d9e-4a94-867d-
                     59d0721fdd51"
                     /created_by="primer3"
                     /Sequence="ACCATGGTGAGCAAGGGCG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-791"
     primer_bind     complement(1183..1201)
                     /%GC="63.2"
                     /Hairpin_Tm="37.1"
                     /Self_Dimer_Tm="13.0"
                     /Tm="62.9"
                     /annotation_group="268228180191200bfec062e-823a-4689-a151-
                     95c8c50f0fc4"
                     /created_by="primer3"
                     /Sequence="CGCCCTTGCTCACCATGGT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-792"
     CDS             1186..1905
                     /kind="reporter"
                     /note="Yellow fluorescent reporter protein - Ex:515,
                     Em:527"
                     /Color="#e1ff2f"
                     /product="SYFP2"
                     /gene="SYFP2"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SYFP2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:dx-enavel9"">SYFP2</a>"
                     /label="SYFP2"
     primer_bind     complement(1186..1206)
                     /%GC="61.9"
                     /Hairpin_Tm="34.4"
                     /Self_Dimer_Tm="None"
                     /Tm="64.5"
                     /annotation_group="267411313157700ab081476-d455-484b-9f16-
                     f7265b92726c"
                     /created_by="primer3"
                     /Sequence="CTCCTCGCCCTTGCTCACCAT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-2595"
     primer_bind     complement(1186..1206)
                     /%GC="61.9"
                     /Hairpin_Tm="34.4"
                     /Self_Dimer_Tm="None"
                     /Tm="64.5"
                     /annotation_group="268225471053600fb284203-12a5-4b6f-aa11-
                     a50fa338630d"
                     /created_by="primer3"
                     /Sequence="CTCCTCGCCCTTGCTCACCAT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-2595"
     primer_bind     1195..1214
                     /%GC="60.0"
                     /Hairpin_Tm="47.3"
                     /Self_Dimer_Tm="None"
                     /Tm="62.5"
                     /annotation_group="2674137644551000debb238-62ff-48b0-84db-
                     2273d95aad00"
                     /created_by="primer3"
                     /Sequence="AAGGGCGAGGAGCTGTTCAC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-667"
     primer_bind     1195..1214
                     /%GC="60.0"
                     /Hairpin_Tm="47.3"
                     /Self_Dimer_Tm="None"
                     /Tm="62.5"
                     /annotation_group="268228007567900bf107f4b-dace-47cf-9678-
                     9d0ce8c6c327"
                     /created_by="primer3"
                     /Sequence="AAGGGCGAGGAGCTGTTCAC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-667"
     primer_bind     1230..1249
                     /%GC="75.0"
                     /Hairpin_Tm="37.8"
                     /Self_Dimer_Tm="30.2"
                     /Tm="67.6"
                     /annotation_group="267412029042400bd640d40-19ee-4d12-9e22-
                     213c87aeb9c3"
                     /created_by="primer3"
                     /Sequence="CCTGGTCGAGCTGGACGGCG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-331"
     primer_bind     1230..1249
                     /%GC="75.0"
                     /Hairpin_Tm="37.8"
                     /Self_Dimer_Tm="30.2"
                     /Tm="67.6"
                     /annotation_group="268226282281000335e9e10-e95a-4203-8c38-
                     08482524df12"
                     /created_by="primer3"
                     /Sequence="CCTGGTCGAGCTGGACGGCG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-331"
     primer_bind     1248..1270
                     /%GC="52.2"
                     /Hairpin_Tm="46.5"
                     /Self_Dimer_Tm="2.2"
                     /Tm="63.9"
                     /annotation_group="2674124959641004c3bdab9-67bb-4058-8635-
                     cfc5da5dc9be"
                     /created_by="primer3"
                     /Sequence="CGACGTAAACGGCCACAAGTTCA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-736"
     primer_bind     1248..1270
                     /%GC="52.2"
                     /Hairpin_Tm="46.5"
                     /Self_Dimer_Tm="2.2"
                     /Tm="63.9"
                     /annotation_group="26822674369520051e099fe-a601-405e-9758-
                     e618b8fdca09"
                     /created_by="primer3"
                     /Sequence="CGACGTAAACGGCCACAAGTTCA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-736"
     primer_bind     1248..1267
                     /%GC="55.0"
                     /Hairpin_Tm="46.5"
                     /Self_Dimer_Tm="2.2"
                     /Tm="61.2"
                     /annotation_group="267414048146100c47d653d-e673-4064-a014-
                     182b5cb2d872"
                     /created_by="primer3"
                     /Sequence="CGACGTAAACGGCCACAAGT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-2645"
     primer_bind     1248..1267
                     /%GC="55.0"
                     /Hairpin_Tm="46.5"
                     /Self_Dimer_Tm="2.2"
                     /Tm="61.2"
                     /annotation_group="268228293463600a2c515e3-a96e-4e46-b3ef-
                     e4b6ec799334"
                     /created_by="primer3"
                     /Sequence="CGACGTAAACGGCCACAAGT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-2645"
     primer_bind     complement(1253..1275)
                     /%GC="52.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="64.2"
                     /annotation_group="2674125527081006f937811-e03e-437a-83f0-
                     1772fa76fe10"
                     /created_by="primer3"
                     /Sequence="CACGCTGAACTTGTGGCCGTTTA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-2462"
     primer_bind     complement(1253..1275)
                     /%GC="52.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="64.2"
                     /annotation_group="26822679981429913c8b7a9-10b5-4fff-acc5-
                     f73c489d02cd"
                     /created_by="primer3"
                     /Sequence="CACGCTGAACTTGTGGCCGTTTA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-2462"
     primer_bind     1254..1273
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="2.2"
                     /Tm="62.1"
                     /annotation_group="2674122629687991b662f0d-b2ff-4b6f-a40b-
                     2c716e0ece3f"
                     /created_by="primer3"
                     /Sequence="AAACGGCCACAAGTTCAGCG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-1027"
     primer_bind     1254..1273
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="2.2"
                     /Tm="62.1"
                     /annotation_group="268226512923799f4a38d0a-b6eb-4653-9e50-
                     42272b630244"
                     /created_by="primer3"
                     /Sequence="AAACGGCCACAAGTTCAGCG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-1027"
     primer_bind     complement(1299..1322)
                     /%GC="58.3"
                     /Hairpin_Tm="58.5"
                     /Self_Dimer_Tm="None"
                     /Tm="66.7"
                     /annotation_group="2674123779128000a63cd81-84ab-46e1-ac7e-
                     3e592aa419bb"
                     /created_by="primer3"
                     /Sequence="TTCAGGGTCAGCTTGCCGTAGGTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-598"
     primer_bind     complement(1299..1322)
                     /%GC="58.3"
                     /Hairpin_Tm="58.5"
                     /Self_Dimer_Tm="None"
                     /Tm="66.7"
                     /annotation_group="2682266283519002c557c85-ad28-417d-b2e6-
                     b28f9797a5e7"
                     /created_by="primer3"
                     /Sequence="TTCAGGGTCAGCTTGCCGTAGGTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-598"
     primer_bind     complement(1300..1319)
                     /%GC="60.0"
                     /Hairpin_Tm="58.5"
                     /Self_Dimer_Tm="None"
                     /Tm="63.4"
                     /annotation_group="26741347531369925c9f147-2bed-450a-b081-
                     a5dae9644517;
                     26741353304070092ae149d-f2a5-4953-8fea-69cc724c05e2"
                     /created_by="primer3"
                     /Sequence="AGGGTCAGCTTGCCGTAGGT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-977; MG-160"
     primer_bind     complement(1300..1319)
                     /%GC="60.0"
                     /Hairpin_Tm="58.5"
                     /Self_Dimer_Tm="None"
                     /Tm="63.4"
                     /annotation_group="26822771877980052ff1eaa-1d5d-46da-bfdb-
                     0fefead0e928;
                     2682277748689004255a9d0-f587-47e5-8056-5fc97ef247ab"
                     /created_by="primer3"
                     /Sequence="AGGGTCAGCTTGCCGTAGGT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-977; MG-160"
     primer_bind     1327..1346
                     /%GC="60.0"
                     /Hairpin_Tm="41.4"
                     /Self_Dimer_Tm="None"
                     /Tm="64.9"
                     /annotation_group="26741278488210043a619de-baac-4f09-82de-
                     9299b2274c97"
                     /created_by="primer3"
                     /Sequence="ATCTGCACCACCGGCAAGCT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-135"
     primer_bind     1327..1346
                     /%GC="60.0"
                     /Hairpin_Tm="41.4"
                     /Self_Dimer_Tm="None"
                     /Tm="64.9"
                     /annotation_group="2682270314221993df7db86-a02a-4d68-92d8-
                     56b071fde0f6"
                     /created_by="primer3"
                     /Sequence="ATCTGCACCACCGGCAAGCT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-135"
     primer_bind     1398..1417
                     /%GC="75.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="67.4"
                     /annotation_group="26741167619720041cc659a-68f5-466d-949f-
                     406d63ae464f;
                     267414329676900b9c185a4-3605-4637-a320-93005b784649"
                     /created_by="primer3"
                     /Sequence="CTTCGCCCGCTACCCCGACC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-548; MG-2641"
     primer_bind     1398..1417
                     /%GC="75.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="67.4"
                     /annotation_group="268225937281800eeef4b57-dc1e-4d30-b300-
                     5040c8295f1a;
                     26822858318680039ab94f4-7e3e-4446-b7b3-97b26f94d73c"
                     /created_by="primer3"
                     /Sequence="CTTCGCCCGCTACCCCGACC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-548; MG-2641"
     primer_bind     complement(1407..1426)
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.5"
                     /annotation_group="2674128985969005a169f75-75c7-4008-95eb-
                     179e70fa2507"
                     /created_by="primer3"
                     /Sequence="GCTTCATGTGGTCGGGGTAG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-3143-ES-R"
     primer_bind     complement(1407..1426)
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.5"
                     /annotation_group="268227144880099862bdb6a-5e90-450b-8719-
                     743f8ac05ea8"
                     /created_by="primer3"
                     /Sequence="GCTTCATGTGGTCGGGGTAG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-3143-ES-R"
     primer_bind     1416..1437
                     /%GC="54.5"
                     /Hairpin_Tm="42.2"
                     /Self_Dimer_Tm="None"
                     /Tm="62.3"
                     /annotation_group="2674136475308003a9a5f3e-4337-4fb4-9d58-
                     2199b166c803"
                     /created_by="primer3"
                     /Sequence="CCACATGAAGCAGCACGACTTC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-2836-MM-F"
     primer_bind     1416..1437
                     /%GC="54.5"
                     /Hairpin_Tm="42.2"
                     /Self_Dimer_Tm="None"
                     /Tm="62.3"
                     /annotation_group="2682278891931990ee60755-286b-4400-a608-
                     49d44f437a6f"
                     /created_by="primer3"
                     /Sequence="CCACATGAAGCAGCACGACTTC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-2836-MM-F"
     primer_bind     1417..1436
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.1"
                     /annotation_group="267411738546499b3885738-5cf7-45f5-b70f-
                     a0e6037f3066"
                     /created_by="primer3"
                     /Sequence="CACATGAAGCAGCACGACTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-129"
     primer_bind     1417..1436
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.1"
                     /annotation_group="2682259951647005c2624f4-3104-49ab-81d7-
                     b3ff611ea54a"
                     /created_by="primer3"
                     /Sequence="CACATGAAGCAGCACGACTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-129"
     primer_bind     complement(1461..1484)
                     /%GC="58.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="27.2"
                     /Tm="66.5"
                     /annotation_group="26741382075440072520115-a4f7-4215-a874-
                     3d463d9bf95b"
                     /created_by="primer3"
                     /Sequence="AAGATGGTGCGCTCCTGGACGTAG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-158"
     primer_bind     complement(1461..1484)
                     /%GC="58.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="27.2"
                     /Tm="66.5"
                     /annotation_group="2682280652442004a9ec3cb-860b-40ae-b470-
                     1cce0714b1a3"
                     /created_by="primer3"
                     /Sequence="AAGATGGTGCGCTCCTGGACGTAG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-158"
     primer_bind     1467..1484
                     /%GC="61.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="26.0"
                     /Tm="59.7"
                     /annotation_group="2674144440330999cf47a6a-d778-4bc0-864e-
                     5dcddc7ac10d"
                     /created_by="primer3"
                     /Sequence="CCAGGAGCGCACCATCTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-111"
     primer_bind     1467..1484
                     /%GC="61.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="26.0"
                     /Tm="59.7"
                     /annotation_group="2682287001684009447d0f6-b139-4dd5-9a48-
                     673a5138554a"
                     /created_by="primer3"
                     /Sequence="CCAGGAGCGCACCATCTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-111"
     primer_bind     complement(1468..1490)
                     /%GC="52.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="27.2"
                     /Tm="63.3"
                     /annotation_group="2674113730263007bb01a25-0cf9-4884-bf4e-
                     2f6763f2e498;
                     2674132447892004aa5b78d-4b12-435b-8296-030ef25cd804"
                     /created_by="primer3"
                     /Sequence="TTGAAGAAGATGGTGCGCTCCTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-777; MG-2467"
     primer_bind     complement(1468..1490)
                     /%GC="52.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="27.2"
                     /Tm="63.3"
                     /annotation_group="2682255293740991c69ecfe-e739-4ec2-8d99-
                     f68330a4abfd;
                     268227489622999d3e6423a-7c81-4fab-8918-133accaf46be"
                     /created_by="primer3"
                     /Sequence="TTGAAGAAGATGGTGCGCTCCTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-777; MG-2467"
     primer_bind     1484..1503
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="1.6"
                     /Tm="60.6"
                     /annotation_group="2674133020390993b496e9d-a602-4a72-9d4b-
                     54ae379a5fc2"
                     /created_by="primer3"
                     /Sequence="TCTTCAAGGACGACGGCAAC"
                     /Mismatches=0
                     /#_Off-target_Sites=1
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA<br>"
                     /Off-target_#1="<html><font
                     color=""red""><b>A</b></font>CTTCAAGGA<font
                     color=""red""><b>G</b></font>GACGGCAAC</html>"
                     /Location_#1="<html>1,339 -&gt; 1,358 (AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA)</html>"
                     /label="MG-2709"
     primer_bind     1484..1503
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="1.6"
                     /Tm="60.6"
                     /annotation_group="268227546899900707ec0b3-fe17-47a1-ac82-
                     dcc31350e42c"
                     /created_by="primer3"
                     /Sequence="TCTTCAAGGACGACGGCAAC"
                     /Mismatches=0
                     /#_Off-target_Sites=1
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA<br>"
                     /Off-target_#1="<html><font
                     color=""red""><b>A</b></font>CTTCAAGGA<font
                     color=""red""><b>G</b></font>GACGGCAAC</html>"
                     /Location_#1="<html>1,339 -&gt; 1,358 (AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA)</html>"
                     /label="MG-2709"
     primer_bind     1501..1520
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="64.3"
                     /annotation_group="26741161711820049095d55-cfe9-47c3-b28b-
                     867056dfbd2a"
                     /created_by="primer3"
                     /Sequence="AACTACAAGACCCGCGCCGA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-627"
     primer_bind     1501..1520
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="64.3"
                     /annotation_group="268225822236299ede3a2d2-7b40-457a-b132-
                     a58a31178ac0"
                     /created_by="primer3"
                     /Sequence="AACTACAAGACCCGCGCCGA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-627"
     primer_bind     complement(1519..1538)
                     /%GC="60.0"
                     /Hairpin_Tm="42.4"
                     /Self_Dimer_Tm="None"
                     /Tm="61.9"
                     /annotation_group="2674134183220000d7df526-d7f2-431e-902b-
                     bf945b0500fe"
                     /created_by="primer3"
                     /Sequence="TCGCCCTCGAACTTCACCTC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-62"
     primer_bind     complement(1519..1538)
                     /%GC="60.0"
                     /Hairpin_Tm="42.4"
                     /Self_Dimer_Tm="None"
                     /Tm="61.9"
                     /annotation_group="268227662973599eb4edfb3-2f79-4399-a647-
                     c36fdb05d642"
                     /created_by="primer3"
                     /Sequence="TCGCCCTCGAACTTCACCTC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-62"
     primer_bind     1572..1591
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="62.2"
                     /annotation_group="2674130725738006e5fc539-a586-425e-9d20-
                     a708e48fbaa4"
                     /created_by="primer3"
                     /Sequence="CGACTTCAAGGAGGACGGCA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-144"
     primer_bind     1572..1591
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="62.2"
                     /annotation_group="26822731525589972790ab7-303b-43c6-bf49-
                     0b1064dc0478"
                     /created_by="primer3"
                     /Sequence="CGACTTCAAGGAGGACGGCA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-144"
     primer_bind     1600..1619
                     /%GC="55.0"
                     /Hairpin_Tm="41.7"
                     /Self_Dimer_Tm="None"
                     /Tm="60.3"
                     /annotation_group="267414387800999594dca57-9329-4ad1-8638-
                     89a44afc8958"
                     /created_by="primer3"
                     /Sequence="GGGCACAAGCTGGAGTACAA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-3140-ES-F"
     primer_bind     1600..1619
                     /%GC="55.0"
                     /Hairpin_Tm="41.7"
                     /Self_Dimer_Tm="None"
                     /Tm="60.3"
                     /annotation_group="26822864026620046a6d84c-d712-456a-81fc-
                     f011d2d94421"
                     /created_by="primer3"
                     /Sequence="GGGCACAAGCTGGAGTACAA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-3140-ES-F"
     primer_bind     1614..1633
                     /%GC="45.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="55.6"
                     /annotation_group="267411854696300d78eb1c8-8e39-4cad-a32f-
                     c93910a9f3fe"
                     /created_by="primer3"
                     /Sequence="GTACAACTACAACAGCCACA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-668"
     primer_bind     1614..1633
                     /%GC="45.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="55.6"
                     /annotation_group="268226111931500efa4420b-5557-45e1-b25d-
                     44c3d2858256"
                     /created_by="primer3"
                     /Sequence="GTACAACTACAACAGCCACA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-668"
     primer_bind     1631..1650
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="57.5"
                     /annotation_group="267412204882500787da284-21cb-489f-8d7b-
                     0b85eac53fa1"
                     /created_by="primer3"
                     /Sequence="ACAACGTCTATATCACCGCC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-332"
     primer_bind     1631..1650
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="57.5"
                     /annotation_group="2682264547963999dd023bd-9bce-4c3f-81b4-
                     da593d987cec"
                     /created_by="primer3"
                     /Sequence="ACAACGTCTATATCACCGCC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-332"
     primer_bind     1680..1702
                     /%GC="52.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="62.3"
                     /annotation_group="267411796101100c06fe419-583b-411b-856c-
                     860c017d311f;
                     26741260922340067be3c63-c47d-47e6-b838-fab263ec1655"
                     /created_by="primer3"
                     /Sequence="CTTCAAGATCCGCCACAACATCG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-776; MG-2748"
     primer_bind     1680..1702
                     /%GC="52.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="62.3"
                     /annotation_group="2682260547438004b9edede-d0fd-4139-a976-
                     003d274901a3;
                     2682268566269990c51739a-e9a5-44a4-a64b-1321e27af678"
                     /created_by="primer3"
                     /Sequence="CTTCAAGATCCGCCACAACATCG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-776; MG-2748"
     primer_bind     complement(1685..1704)
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.2"
                     /annotation_group="26741266608100013e31c0d-64a6-4768-86c3-
                     b2d00966b446"
                     /created_by="primer3"
                     /Sequence="CTCGATGTTGTGGCGGATCT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-3141-ES-R"
     primer_bind     complement(1685..1704)
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.2"
                     /annotation_group="268226914367000a3e191c5-0e97-4262-beb3-
                     a54391d74a4a"
                     /created_by="primer3"
                     /Sequence="CTCGATGTTGTGGCGGATCT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-3141-ES-R"
     primer_bind     1688..1707
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="3.1"
                     /Tm="62.5"
                     /annotation_group="26741155675870074c820e8-79b6-4791-a7f2-
                     8abf52b11b86"
                     /created_by="primer3"
                     /Sequence="TCCGCCACAACATCGAGGAC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-1026"
     primer_bind     1688..1707
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="3.1"
                     /Tm="62.5"
                     /annotation_group="268225763938300f1ac59da-bc30-4f61-8c0e-
                     4092b5d42c4a"
                     /created_by="primer3"
                     /Sequence="TCCGCCACAACATCGAGGAC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-1026"
     primer_bind     1716..1735
                     /%GC="70.0"
                     /Hairpin_Tm="32.9"
                     /Self_Dimer_Tm="None"
                     /Tm="65.6"
                     /annotation_group="267411971228300580948ea-3b41-4250-a89d-
                     89c4f2fff2a6"
                     /created_by="primer3"
                     /Sequence="GCAGCTCGCCGACCACTACC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-324"
     primer_bind     1716..1735
                     /%GC="70.0"
                     /Hairpin_Tm="32.9"
                     /Self_Dimer_Tm="None"
                     /Tm="65.6"
                     /annotation_group="26822622525740069f2e1e0-43ed-45fb-8dce-
                     b7b445f6303c"
                     /created_by="primer3"
                     /Sequence="GCAGCTCGCCGACCACTACC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-324"
     primer_bind     complement(1727..1749)
                     /%GC="60.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="66.2"
                     /annotation_group="2674129550655007058dc41-94fc-4246-bb26-
                     f7dc9e4dc535"
                     /created_by="primer3"
                     /Sequence="GGGGGTGTTCTGCTGGTAGTGGT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-737"
     primer_bind     complement(1727..1749)
                     /%GC="60.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="66.2"
                     /annotation_group="268227201736200aaf7f386-f92b-476e-89c8-
                     9bef161a7aab"
                     /created_by="primer3"
                     /Sequence="GGGGGTGTTCTGCTGGTAGTGGT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-737"
     primer_bind     1729..1748
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.3"
                     /annotation_group="26741284245720067b129d7-f33c-485b-a75c-
                     5c19e45501ff"
                     /created_by="primer3"
                     /Sequence="CACTACCAGCAGAACACCCC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-136"
     primer_bind     1729..1748
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.3"
                     /annotation_group="268227087475700f3fefa7b-7755-46fb-ab46-
                     1df0a7a67e58"
                     /created_by="primer3"
                     /Sequence="CACTACCAGCAGAACACCCC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-136"
     primer_bind     1809..1831
                     /%GC="56.5"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="64.0"
                     /annotation_group="267411436407100fc92f5b3-d91d-411e-9566-
                     fede56321fae"
                     /created_by="primer3"
                     /Sequence="GAGCAAAGACCCCAACGAGAAGC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-2412"
     primer_bind     1809..1831
                     /%GC="56.5"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="64.0"
                     /annotation_group="268225645089600c6605147-5d5a-4988-a769-
                     1f67bd82e174"
                     /created_by="primer3"
                     /Sequence="GAGCAAAGACCCCAACGAGAAGC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-2412"
     primer_bind     1811..1830
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.1"
                     /annotation_group="267413989304500b0e426d5-4535-4cab-833d-
                     924bf9f9a6db"
                     /created_by="primer3"
                     /Sequence="GCAAAGACCCCAACGAGAAG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-2243"
     primer_bind     1811..1830
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.1"
                     /annotation_group="268228236933399a31f7016-0edd-4148-b946-
                     f67d1de804b7"
                     /created_by="primer3"
                     /Sequence="GCAAAGACCCCAACGAGAAG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-2243"
     primer_bind     complement(1821..1843)
                     /%GC="56.5"
                     /Hairpin_Tm="41.1"
                     /Self_Dimer_Tm="5.7"
                     /Tm="64.7"
                     /annotation_group="26741243820099918a335b0-9ce8-48b1-8d20-
                     265c456ad293"
                     /created_by="primer3"
                     /Sequence="CCATGTGATCGCGCTTCTCGTTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-170"
     primer_bind     complement(1821..1843)
                     /%GC="56.5"
                     /Hairpin_Tm="41.1"
                     /Self_Dimer_Tm="5.7"
                     /Tm="64.7"
                     /annotation_group="2682266872436000f179363-792a-4ef1-9837-
                     1a3f9f1d687c"
                     /created_by="primer3"
                     /Sequence="CCATGTGATCGCGCTTCTCGTTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-170"
     primer_bind     1824..1843
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="12.1"
                     /Tm="61.8"
                     /annotation_group="2674131295933006b0e11fe-d86f-4b9c-a9e3-
                     2860252ed02e"
                     /created_by="primer3"
                     /Sequence="CGAGAAGCGCGATCACATGG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-326"
     primer_bind     1824..1843
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="12.1"
                     /Tm="61.8"
                     /annotation_group="26822737466029900b3a073-3790-4502-b192-
                     36c042bda29b"
                     /created_by="primer3"
                     /Sequence="CGAGAAGCGCGATCACATGG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-326"
     primer_bind     1833..1855
                     /%GC="56.5"
                     /Hairpin_Tm="44.2"
                     /Self_Dimer_Tm="13.4"
                     /Tm="64.4"
                     /annotation_group="267412147271799645f147c-c487-4b31-af70-
                     9017e37e63fc"
                     /created_by="primer3"
                     /Sequence="CGATCACATGGTCCTGCTGGAGT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-2410"
     primer_bind     1833..1855
                     /%GC="56.5"
                     /Hairpin_Tm="44.2"
                     /Self_Dimer_Tm="13.4"
                     /Tm="64.4"
                     /annotation_group="2682263981515997f1c9153-b8d1-42f7-b8e0-
                     9fa3b10ad0c2"
                     /created_by="primer3"
                     /Sequence="CGATCACATGGTCCTGCTGGAGT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-2410"
     misc_feature    1940..2185
                     /vntifkey=21
                     /locus_tag="WPRE3"
                     /ApEinfo_label="WPRE3"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="WPRE3"
     primer_bind     complement(1999..2018)
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="56.6"
                     /annotation_group="267413876751800df0f669b-8247-4d6d-80b3-
                     703064036014"
                     /created_by="primer3"
                     /Sequence="CCACATAGCGTAAAAGGAGC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-110"
     primer_bind     complement(1999..2018)
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="56.6"
                     /annotation_group="268228123244100c0fee5a7-fbe6-49a7-b728-
                     8a56da26ac5d"
                     /created_by="primer3"
                     /Sequence="CCACATAGCGTAAAAGGAGC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-110"
     primer_bind     complement(2012..2031)
                     /%GC="40.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="54.3"
                     /annotation_group="26741421708039952f59c31-0fb9-4682-aa8d-
                     1a7865294f61"
                     /created_by="primer3"
                     /Sequence="TAAAGCAGCGTATCCACATA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-2396"
     primer_bind     complement(2012..2031)
                     /%GC="40.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="54.3"
                     /annotation_group="268228469072400601863b9-5e7e-4c17-ba3c-
                     ca48737cbb47"
                     /created_by="primer3"
                     /Sequence="TAAAGCAGCGTATCCACATA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-2396"
     polyA_signal    2199..2402
                     /vntifkey=21
                     /locus_tag="BGH-pA"
                     /ApEinfo_label="BGH-pA"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /modified_by="jacqueline.bendrick"
                     /label="bGHpA"
     primer_bind     2207..2226
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="58.3"
                     /annotation_group="267414272616799c4c23654-ead2-4f8e-b89e-
                     9053ecad5863"
                     /created_by="primer3"
                     /Sequence="CCTTCTAGTTGCCAGCCATC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-241"
     primer_bind     2207..2226
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="58.3"
                     /annotation_group="26822852565149949ce2de6-0b33-4bcc-b8a2-
                     85cb67ab4eb6"
                     /created_by="primer3"
                     /Sequence="CCTTCTAGTTGCCAGCCATC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-241"
     primer_bind     2214..2236
                     /%GC="52.2"
                     /Hairpin_Tm="38.7"
                     /Self_Dimer_Tm="None"
                     /Tm="63.7"
                     /annotation_group="267413012870599e58ca855-2c58-40fc-adb5-
                     390e7b9bb3b3;
                     2674133603596002949e7ed-af4c-40f7-9b8d-6cedb4be51fd;
                     26741416083720081a74d07-1058-4216-9c44-7185e88b24bd"
                     /created_by="primer3"
                     /Sequence="GTTGCCAGCCATCTGTTGTTTGC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="scAAV_R-ITR_2; MG-2274; MG-729"
     primer_bind     complement(2214..2236)
                     /%GC="52.2"
                     /Hairpin_Tm="46.6"
                     /Self_Dimer_Tm="8.4"
                     /Tm="63.7"
                     /annotation_group="2674131877977008b3794d1-e75b-42d0-b831-
                     b0acb4ec6f8f"
                     /created_by="primer3"
                     /Sequence="GCAAACAACAGATGGCTGGCAAC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-523"
     primer_bind     2214..2236
                     /%GC="52.2"
                     /Hairpin_Tm="38.7"
                     /Self_Dimer_Tm="None"
                     /Tm="63.7"
                     /annotation_group="26822725786860078ab4fd2-112c-491b-9ee6-
                     8eaaec938b49;
                     268227603505200edb06520-8f9e-409e-a4fa-680651cd2359;
                     268228410174000e6eef936-3117-4426-bf0e-717c22380831"
                     /created_by="primer3"
                     /Sequence="GTTGCCAGCCATCTGTTGTTTGC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="scAAV_R-ITR_2; MG-2274; MG-729"
     primer_bind     complement(2214..2236)
                     /%GC="52.2"
                     /Hairpin_Tm="46.6"
                     /Self_Dimer_Tm="8.4"
                     /Tm="63.7"
                     /annotation_group="268227432841199b57e857f-e712-424b-a54c-
                     8ed640e52bd4"
                     /created_by="primer3"
                     /Sequence="GCAAACAACAGATGGCTGGCAAC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-523"
     primer_bind     2254..2276
                     /%GC="60.9"
                     /Hairpin_Tm="59.5"
                     /Self_Dimer_Tm="None"
                     /Tm="65.1"
                     /annotation_group="267412722872400e05fe536-90c7-4883-aa3c-
                     4808b96cd011"
                     /created_by="primer3"
                     /Sequence="CTTGACCCTGGAAGGTGCCACTC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-887"
     primer_bind     2254..2276
                     /%GC="60.9"
                     /Hairpin_Tm="59.5"
                     /Self_Dimer_Tm="None"
                     /Tm="65.1"
                     /annotation_group="268226971296900c620b8d3-cda2-410f-a354-
                     cf1aa692e207"
                     /created_by="primer3"
                     /Sequence="CTTGACCCTGGAAGGTGCCACTC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-887"
     primer_bind     complement(2264..2286)
                     /%GC="60.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="15.3"
                     /Tm="66.2"
                     /annotation_group="26741124988350069fe70ef-ae51-4502-9522-
                     5a38e5bc8d6a;
                     26741370456980024cd7320-681b-45f2-a474-ac75928abfc0"
                     /created_by="primer3"
                     /Sequence="AGGACAGTGGGAGTGGCACCTTC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-1080; MG-2202"
     primer_bind     complement(2264..2286)
                     /%GC="60.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="15.3"
                     /Tm="66.2"
                     /annotation_group="26822541279420001004aac-b7e1-460d-b5f4-
                     2b788c74c767;
                     2682279475903004bfbf0d5-a4d7-4195-9073-4d0e6d1366dd"
                     /created_by="primer3"
                     /Sequence="AGGACAGTGGGAGTGGCACCTTC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-1080; MG-2202"
     primer_bind     complement(2278..2297)
                     /%GC="35.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="50.0"
                     /annotation_group="26741208920190009ce0483-fa05-4fc5-a158-
                     4b40eb6ce6db"
                     /created_by="primer3"
                     /Sequence="TTTATTAGGAAAGGACAGTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-65"
     primer_bind     complement(2278..2297)
                     /%GC="35.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="50.0"
                     /annotation_group="268226340177799dec06043-766e-4f35-9181-
                     640bf198a070"
                     /created_by="primer3"
                     /Sequence="TTTATTAGGAAAGGACAGTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-65"
     primer          complement(2313..2332)
                     /vntifkey=27
                     /locus_tag="BGH-R"
                     /ApEinfo_label="BGH-R"
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#80ff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BGH-R"
     primer_bind     2363..2382
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.4"
                     /annotation_group="267414104542800b15fa760-bcad-438c-b07e-
                     1508bdd307ab"
                     /created_by="primer3"
                     /Sequence="GACAGCAAGGGGGAGGATTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-321"
     primer_bind     2363..2382
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.4"
                     /annotation_group="26822835333540047e91c64-215f-4be9-9532-
                     642405644933"
                     /created_by="primer3"
                     /Sequence="GACAGCAAGGGGGAGGATTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-321"
     primer_bind     2369..2391
                     /%GC="52.2"
                     /Hairpin_Tm="34.4"
                     /Self_Dimer_Tm="None"
                     /Tm="63.2"
                     /annotation_group="267411912744399c913542d-b8d6-4c96-a641-
                     a2b082cc4965;
                     267412320566600e66f0869-d6b5-4a19-9fae-005b7d688bf1;
                     2674135903643006bda018c-134d-4626-8506-1cd8a7b59390"
                     /created_by="primer3"
                     /Sequence="AAGGGGGAGGATTGGGAAGACAA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-886; MG-2701; scAAV_R-ITR_1"
     primer_bind     2369..2391
                     /%GC="52.2"
                     /Hairpin_Tm="34.4"
                     /Self_Dimer_Tm="None"
                     /Tm="63.2"
                     /annotation_group="268226169537300da9461bc-4231-47e8-be6e-
                     b055cd855309;
                     268226570018000f6fa4a20-74dd-4451-9fac-455f1124635b;
                     268227831740000ee4ed0cc-a4e5-45a3-834d-ef604ba52848"
                     /created_by="primer3"
                     /Sequence="AAGGGGGAGGATTGGGAAGACAA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="MG-886; MG-2701; scAAV_R-ITR_1"
     repeat_region   2431..2571
                     /note="adeno-associated virus (AAV-2) inverted terminal
                     repeat (ITR) region required for genome multiplication"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AAV2 ITR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:2m2-enavet2"">AAV2 ITR</a>"
                     /label="AAV2 ITR"
     misc_feature    2431..2571
                     /vntifkey=21
                     /locus_tag="R-ITR"
                     /ApEinfo_label="R-ITR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="R-ITR"
     rep_origin      2646..3101
                     /note="f1 replication origin derived from bacteriophage
                     f1"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="F1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:2jn-enavesu"">F1</a>"
                     /label="F1"
     primer_bind     complement(2654..2673)
                     /%GC="60.0"
                     /Hairpin_Tm="71.3"
                     /Self_Dimer_Tm="24.5"
                     /Tm="64.4"
                     /annotation_group="2682255868786006a6b12ce-306b-49f3-9563-
                     8d386c1fc9ac;
                     268225880054700337427b4-7798-4d71-b9c6-5edb676fe5e5"
                     /created_by="primer3"
                     /Sequence="GCGCTTAATGCGCCGCTACA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="AiP1270 -
                     pAAV-eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA"
                     /label="Npy-IRES2-Flpo\C - MG-290; MG-290"
     rep_origin      2663..2969
                     /vntifkey=33
                     /locus_tag="f1\Origin"
                     /ApEinfo_label="f1\Origin"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /modified_by="jacqueline.bendrick"
                     /label="f1 Origin"
     promoter        3383..3487
                     /note="Promoter for ampicillin resistance gene
                     (b-lactamase)"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="amp"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1jv-enaveop"">amp</a>"
                     /label="amp"
     misc_feature    3488..4345
                     /vntifkey=21
                     /locus_tag="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="AmpR"
     rep_origin      4496..5163
                     /vntifkey=33
                     /locus_tag="pUC\ori"
                     /ApEinfo_label="pUC\ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="pUC\ori"
     primer          5114..5133
                     /created_by="brooke.wynalda"
                     /label="MG_3012"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgctatt gttcctggct gtgcacagct ctgtgctgcc
      181 cacaccccac actttttcag aggctctttg gctaaggaga aggtgggggt cggaacaagc
      241 cacttctcat tttctttaac cagcccaagg agaaacagag catggatttc tgagagtctc
      301 ataattaaat gaattaaaat gttttccttc gtggtgactt tgccaactcc catccacccc
      361 tctttggccc tgttcccaga gagtggcaga gctgggagga tgcagaatga catgttggct
      421 ccaaggggtg agcaggggtg cctccagagc aattagctta tgcatcatca ttctggggca
      481 ggggtgggga tttatggcta gccttcagag cattccagcc cagaccttct cagtccagat
      541 ctttttgctg gagacgttcc tggaagcaga agctgggtat tcattccaat cgcagtcaga
      601 tgggagccca ttcataagat agctgtccac aggctcctct cctgtcactc cagcctgacc
      661 ttgagccacc agcgctgtca aggaggaaaa cattgggctc aaccgcagcc ttcccggcag
      721 gcctaagtgg caacagagga agtcacgtag aagaatggga tgtgacggcc taatggctca
      781 gcatctctag tctgggtgtc atcctcaata tcctcttcac attcttctgc cagggaggtg
      841 acatgcaatg gattatgcct gtggtctgaa acctcccagg ccccagggcc accctttgtc
      901 ttagtttctg aagtggtctc tcttgttcta gagtttcagt ccaagtttgg gatgagccac
      961 tgaagctttc aaaggccagg ttaggtagga agccaaagcc caccacggtc cccttctgac
     1021 ctgaggccac aactcctggg actccctaat gagtttggct gccagtaacg ctatttcact
     1081 gagtttgagc tcgggctggg cataaaagtc agggcagagc catctattgc ttacatttgc
     1141 ttctgggatc cagatctttc gaagctagcg ctaccggtcg ccaccatggt gagcaagggc
     1201 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     1261 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     1321 aagctgatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     1381 ggctacggcg tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc
     1441 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     1501 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     1561 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     1621 tacaacagcc acaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggccaac
     1681 ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag
     1741 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag
     1801 tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     1861 accgccgccg ggatcactct cggcatggac gagctgtaca agtaagtcga cggcgcgccg
     1921 cggccgcgaa ttcgatatca taatcaacct ctggattaca aaatttgtga aagattgact
     1981 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     2041 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggtta
     2101 gttcttgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     2161 ctgttgggca ctgacaattc cgtggctcga gagatcttcg actgtgcctt ctagttgcca
     2221 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     2281 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     2341 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     2401 tgagatctca cgtgcggacc gagcggccgc aggaacccct agtgatggag ttggccactc
     2461 cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg
     2521 gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga
     2581 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc
     2641 atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     2701 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     2761 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     2821 atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag
     2881 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     2941 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcgggct attcttttga
     3001 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     3061 atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac
     3121 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
     3181 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     3241 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
     3301 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     3361 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     3421 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     3481 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     3541 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     3601 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     3661 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     3721 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     3781 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     3841 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     3901 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     3961 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     4021 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     4081 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     4141 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     4201 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     4261 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     4321 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     4381 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     4441 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     4501 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     4561 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     4621 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     4681 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     4741 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     4801 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     4861 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     4921 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     4981 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     5041 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     5101 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     5161 tcacatgt
//
