LOCUS       Exported                5574 bp ds-DNA     circular UNA 01-MAY-2024
DEFINITION  pAAV-3xSP10ins-AiE0219h_3xC2-minRho-SYFP2-8X2C-WPRE-BGHpA.
ACCESSION   Circular
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5574)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 12, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5574
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     LTR             complement(1..141)
                     /label=ITR
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     ITR"
                     /note="/Transferred_From=ITR"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=yoav.ben-simon"
     misc_feature    163..312
                     /label=3xSP10 core insulator
                     /label=nonstandard type:  Insulator
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     3xSP10 core insulator"
                     /note="/Transferred_From=3xSP10 core insulator"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     3xSP10 core insulator"
                     /note="/X-Transferred_From=3xSP10 core insulator"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/modified_by=yoav.ben-simon"
     misc_feature    319..999
                     /label=AiE0219h_3xC2
                     /label=nonstandard type:  Bashed_enhancer
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     AiE0219h_3xC 2"
                     /note="/Transferred_From=AiE0219h_3xC2"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     AiE0219h_3xC 2"
                     /note="/X-Transferred_From=AiE0219h_3xC2"
                     /note="/X-Transferred_Similarity=100.00%"
     promoter        1006..1125
                     /label=MinRho
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=98.33%; Adjusted-Interval-0:0/0; 
                     MinRho
                     (Rho basal promoter)"
                     /note="/Transferred_From=Rho basal promoter"
                     /note="/Transferred_Similarity=98.33%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     Rho basal promoter"
                     /note="/X-Transferred_From=Rho basal promoter"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/modified_by=yoav.ben-simon"
     regulatory      1160..1169
                     /label=Kozak sequence
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     Kozak sequence"
                     /note="/Transferred_From=Kozak sequence"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     Kozak sequence"
                     /note="/X-Transferred_From=Kozak sequence"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/modified_by=yoav.ben-simon"
     CDS             1166..1885
                     /label=SYFP2
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     SYFP2"
                     /note="/Transferred_From=SYFP2"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=yoav.ben-simon"
     regulatory      1881..2342
                     /label=8X2C
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; 
                     Adjusted-Interval-0:199/-201;
                     1X2C (1X2C )"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     8X2C (8X2C )"
                     /note="/Transferred_From=8X2C "
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=yoav.ben-simon"
     misc_feature    1900..1920
                     /label=miRNA128 recognition sequence
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     miRNA128 recognition sequence"
                     /note="/Transferred_From=miRNA128 recognition sequence"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     miRNA128 recognition sequence"
                     /note="/X-Transferred_From=miRNA128 recognition sequence"
                     /note="/X-Transferred_Similarity=100.00%"
     misc_feature    1926..1946
                     /label=miRNA128 recognition sequence
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     miRNA128 recognition sequence"
                     /note="/Transferred_From=miRNA128 recognition sequence"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     miRNA128 recognition sequence"
                     /note="/X-Transferred_From=miRNA128 recognition sequence"
                     /note="/X-Transferred_Similarity=100.00%"
     misc_feature    1952..1972
                     /label=miRNA128 recognition sequence
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     miRNA128 recognition sequence"
                     /note="/Transferred_From=miRNA128 recognition sequence"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     miRNA128 recognition sequence"
                     /note="/X-Transferred_From=miRNA128 recognition sequence"
                     /note="/X-Transferred_Similarity=100.00%"
     misc_feature    1978..1998
                     /label=miRNA128 recognition sequence
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     miRNA128 recognition sequence"
                     /note="/Transferred_From=miRNA128 recognition sequence"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     miRNA128 recognition sequence"
                     /note="/X-Transferred_From=miRNA128 recognition sequence"
                     /note="/X-Transferred_Similarity=100.00%"
     misc_feature    2007..2027
                     /label=miRNA128 recognition sequence
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     miRNA128 recognition sequence"
                     /note="/Transferred_From=miRNA128 recognition sequence"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     miRNA128 recognition sequence"
                     /note="/X-Transferred_From=miRNA128 recognition sequence"
                     /note="/X-Transferred_Similarity=100.00%"
     misc_feature    2033..2053
                     /label=miRNA128 recognition sequence
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     miRNA128 recognition sequence"
                     /note="/Transferred_From=miRNA128 recognition sequence"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     miRNA128 recognition sequence"
                     /note="/X-Transferred_From=miRNA128 recognition sequence"
                     /note="/X-Transferred_Similarity=100.00%"
     misc_feature    2059..2079
                     /label=miRNA128 recognition sequence
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     miRNA128 recognition sequence"
                     /note="/Transferred_From=miRNA128 recognition sequence"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     miRNA128 recognition sequence"
                     /note="/X-Transferred_From=miRNA128 recognition sequence"
                     /note="/X-Transferred_Similarity=100.00%"
     misc_feature    2085..2105
                     /label=miRNA128 recognition sequence
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     miRNA128 recognition sequence"
                     /note="/Transferred_From=miRNA128 recognition sequence"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     miRNA128 recognition sequence"
                     /note="/X-Transferred_From=miRNA128 recognition sequence"
                     /note="/X-Transferred_Similarity=100.00%"
     misc_feature    2114..2136
                     /label=miR221 recognition sequence
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     miR221 recognition sequence"
                     /note="/Transferred_From=miR221 recognition sequence"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     miR221 recognition sequence"
                     /note="/X-Transferred_From=miR221 recognition sequence"
                     /note="/X-Transferred_Similarity=100.00%"
     misc_feature    2142..2164
                     /label=miR221 recognition sequence
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     miR221 recognition sequence"
                     /note="/Transferred_From=miR221 recognition sequence"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     miR221 recognition sequence"
                     /note="/X-Transferred_From=miR221 recognition sequence"
                     /note="/X-Transferred_Similarity=100.00%"
     misc_feature    2170..2192
                     /label=miR221 recognition sequence
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     miR221 recognition sequence"
                     /note="/Transferred_From=miR221 recognition sequence"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     miR221 recognition sequence"
                     /note="/X-Transferred_From=miR221 recognition sequence"
                     /note="/X-Transferred_Similarity=100.00%"
     misc_feature    2198..2220
                     /label=miR221 recognition sequence
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     miR221 recognition sequence"
                     /note="/Transferred_From=miR221 recognition sequence"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     miR221 recognition sequence"
                     /note="/X-Transferred_From=miR221 recognition sequence"
                     /note="/X-Transferred_Similarity=100.00%"
     misc_feature    2229..2251
                     /label=miR221 recognition sequence
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     miR221 recognition sequence"
                     /note="/Transferred_From=miR221 recognition sequence"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     miR221 recognition sequence"
                     /note="/X-Transferred_From=miR221 recognition sequence"
                     /note="/X-Transferred_Similarity=100.00%"
     misc_feature    2257..2279
                     /label=miR221 recognition sequence
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     miR221 recognition sequence"
                     /note="/Transferred_From=miR221 recognition sequence"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     miR221 recognition sequence"
                     /note="/X-Transferred_From=miR221 recognition sequence"
                     /note="/X-Transferred_Similarity=100.00%"
     misc_feature    2285..2307
                     /label=miR221 recognition sequence
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     miR221 recognition sequence"
                     /note="/Transferred_From=miR221 recognition sequence"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     miR221 recognition sequence"
                     /note="/X-Transferred_From=miR221 recognition sequence"
                     /note="/X-Transferred_Similarity=100.00%"
     misc_feature    2313..2335
                     /label=miR221 recognition sequence
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     miR221 recognition sequence"
                     /note="/Transferred_From=miR221 recognition sequence"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     miR221 recognition sequence"
                     /note="/X-Transferred_From=miR221 recognition sequence"
                     /note="/X-Transferred_Similarity=100.00%"
     3'UTR           2346..2591
                     /label=WPRE3
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     WPRE3"
                     /note="/Transferred_From=WPRE3"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=yoav.ben-simon"
                     /note="/modified_by=yoav.ben-simon"
     polyA_signal    2605..2808
                     /label=BGH-pA
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     BGH-pA"
                     /note="/Transferred_From=BGH-pA"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=yoav.ben-simon"
     LTR             2837..2977
                     /label=ITR
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     ITR"
                     /note="/Transferred_From=ITR"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=yoav.ben-simon"
     rep_origin      3069..3375
                     /label=f1 Origin
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; f1 
                     Origin"
                     /note="/Transferred_From=f1 Origin"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     f1 Origin"
                     /note="/X-Transferred_From=f1 Origin"
                     /note="/X-Transferred_Similarity=100.00%"
     promoter        3789..3893
                     /gene="bla"
                     /label=AmpR promoter
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     AmpR promoter"
                     /note="/Transferred_From=AmpR promoter"
                     /note="/Transferred_Similarity=100.00%"
     misc_feature    3824..3831
                     /label=FRT3 spacer (FASEBJ 2011, 25, 1)
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     FRT3 spacer (FASEBJ 2011, 25, 1)"
                     /note="/Transferred_From=FRT3 spacer (FASEBJ 2011, 25, 1)"
                     /note="/Transferred_Similarity=100.00%"
     CDS             3894..4751
                     /label=AmpR
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     AmpR"
                     /note="/Transferred_From=AmpR"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=yoav.ben-simon"
                     /note="/modified_by=yoav.ben-simon"
     rep_origin      4902..5569
                     /label=pUC ori
                     /label=pUC\ori
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     pUC\ori
                     (pUC ori)"
                     /note="/Transferred_From=pUC ori"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/vntifkey=33"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgccggta ccgaagctac ccctaacaca
      181 ctattctaca cacagaaaat gctcttcact aggaagctac ccctaacaca ctattctaca
      241 cacagaaaat gctcttcact aggaagctac ccctaacaca ctattctaca cacagaaaat
      301 gctcttcact agacgcgttg gtcttgatca attgtcccag cagagttggt gcatgggaaa
      361 gcctttcttg gccttatcag cagctcagcc tgggaggttg gcccgggggc tgaccaagca
      421 caaaatagaa aagccactaa taagatccag ctggaacaaa tagcctctat ttattttagg
      481 aaactaatga tatgtcatca tgacacatat ctaaccttag tatttctgat tggacaggcc
      541 agttatggtc ttgatcaatt gtcccagcag agttggtgca tgggaaagcc tttcttggcc
      601 ttatcagcag ctcagcctgg gaggttggcc cgggggctga ccaagcacaa aatagaaaag
      661 ccactaataa gatccagctg gaacaaatag cctctattta ttttaggaaa ctaatgatat
      721 gtcatcatga cacatatcta accttagtat ttctgattgg acaggccagt tatggtcttg
      781 atcaattgtc ccagcagagt tggtgcatgg gaaagccttt cttggcctta tcagcagctc
      841 agcctgggag gttggcccgg gggctgacca agcacaaaat agaaaagcca ctaataagat
      901 ccagctggaa caaatagcct ctatttattt taggaaacta atgatatgtc atcatgacac
      961 atatctaacc ttagtatttc tgattggaca ggccagttag agctcgattc agccgggagc
     1021 ttagggaggg gaggtcactt cataagggct tgggggggga gttggagcca cgagtcgtcc
     1081 agccggagcc ccgtgtggct gtgctccggc ctcagaagca tccccggatc cagatctttc
     1141 gaagctagcg ctaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt
     1201 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     1261 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagctgatct gcaccaccgg
     1321 caagctgccc gtgccctggc ccaccctcgt gaccaccctg ggctacggcg tgcagtgctt
     1381 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     1441 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     1501 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     1561 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     1621 tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat
     1681 cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     1741 ccccgtgctg ctgcccgaca accactacct gagctaccag tccaagctga gcaaagaccc
     1801 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     1861 cggcatggac gagctgtaca agtaaactag tgcggcctta aagagaccgg ttcactgtga
     1921 cagtaaaaga gaccggttca ctgtgagaat gaaagagacc ggttcactgt gatcggaaaa
     1981 gagaccggtt cactgtgagc ggccttaaag agaccggttc actgtgacag taaaagagac
     2041 cggttcactg tgagaatgaa agagaccggt tcactgtgat cggaaaagag accggttcac
     2101 tgtgagcggc cttgaaaccc agcagacaat gtagctcagt agaaacccag cagacaatgt
     2161 agctgaatgg aaacccagca gacaatgtag cttcggagaa acccagcaga caatgtagct
     2221 gcggccttga aacccagcag acaatgtagc tcagtagaaa cccagcagac aatgtagctg
     2281 aatggaaacc cagcagacaa tgtagcttcg gagaaaccca gcagacaatg tagctgcggc
     2341 cgatcataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     2401 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     2461 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttagttc ttgccacggc
     2521 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     2581 caattccgtg gctcgagaga tcttcgactg tgccttctag ttgccagcca tctgttgttt
     2641 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     2701 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     2761 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgag atctcacgtg
     2821 cggaccgagc ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct
     2881 cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg
     2941 gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc
     3001 cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct
     3061 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     3121 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     3181 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     3241 ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct
     3301 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     3361 tccaaactgg aacaacactc aaccctatct cgggctattc ttttgattta taagggattt
     3421 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     3481 ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg
     3541 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     3601 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     3661 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     3721 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     3781 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     3841 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     3901 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     3961 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     4021 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     4081 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     4141 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     4201 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     4261 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     4321 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     4381 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     4441 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     4501 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     4561 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     4621 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     4681 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     4741 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     4801 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     4861 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     4921 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     4981 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     5041 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     5101 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     5161 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     5221 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     5281 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     5341 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     5401 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     5461 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     5521 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgt
//
