LOCUS       Exported                4737 bp ds-DNA     circular UNA 01-MAY-2024
DEFINITION  pAAV-AiE0499m-minBG-SYFP2-WPRE-BGHpA.
ACCESSION   Circular
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 12, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4737
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     LTR             complement(1..141)
                     /label=ITR
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     ITR"
                     /note="/Transferred_From=ITR"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=yoav.ben-simon"
     enhancer        354..656
                     /label=AiE0499m
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     AiE0499m "
                     /note="/Transferred_From=AiE0499m"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     AiE0499m "
                     /note="/X-Transferred_From=AiE0499m"
                     /note="/X-Transferred_Similarity=100.00%"
     promoter        662..714
                     /label=minBG promoter
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     minBG
                     promoter (Beta Globin minimal promoter)"
                     /note="/Transferred_From=Beta Globin minimal promoter"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=yoav.ben-simon"
                     /note="/modified_by=yoav.ben-simon"
     regulatory      749..758
                     /label=Kozak sequence
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     Kozak sequence"
                     /note="/Transferred_From=Kozak sequence"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     Kozak sequence"
                     /note="/X-Transferred_From=Kozak sequence"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/modified_by=yoav.ben-simon"
     CDS             755..1474
                     /label=SYFP2
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     SYFP2"
                     /note="/Transferred_From=SYFP2"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=yoav.ben-simon"
     3'UTR           1509..1754
                     /label=WPRE3
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     WPRE3"
                     /note="/Transferred_From=WPRE3"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=yoav.ben-simon"
                     /note="/modified_by=yoav.ben-simon"
     polyA_signal    1768..1971
                     /label=BGH-pA
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     BGH-pA"
                     /note="/Transferred_From=BGH-pA"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=yoav.ben-simon"
     LTR             2000..2140
                     /label=ITR
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     ITR"
                     /note="/Transferred_From=ITR"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=yoav.ben-simon"
     rep_origin      2232..2538
                     /label=f1 Origin
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; f1 
                     Origin"
                     /note="/Transferred_From=f1 Origin"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     f1 Origin"
                     /note="/X-Transferred_From=f1 Origin"
                     /note="/X-Transferred_Similarity=100.00%"
     promoter        2952..3056
                     /gene="bla"
                     /label=AmpR promoter
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     AmpR promoter"
                     /note="/Transferred_From=AmpR promoter"
                     /note="/Transferred_Similarity=100.00%"
     misc_feature    2987..2994
                     /label=FRT3 spacer (FASEBJ 2011, 25, 1)
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     FRT3 spacer (FASEBJ 2011, 25, 1)"
                     /note="/Transferred_From=FRT3 spacer (FASEBJ 2011, 25, 1)"
                     /note="/Transferred_Similarity=100.00%"
     CDS             3057..3914
                     /label=AmpR
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     AmpR"
                     /note="/Transferred_From=AmpR"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=yoav.ben-simon"
                     /note="/modified_by=yoav.ben-simon"
     rep_origin      4065..4732
                     /label=pUC ori
                     /label=pUC\ori
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     pUC\ori
                     (pUC ori)"
                     /note="/Transferred_From=pUC ori"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/vntifkey=33"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtcatgc cccggctgca tcccggccgc
      181 cccggcgagc cggcggcaag gggcgcgaga ggccgggttg gagcggcggt ggctgccgcg
      241 gcgtctccgg agaccacgcg agctgctggg gcaggcgccc gactgctgtc cgaggaggtt
      301 tccgccggtt ccggagcaac ggctccgtgt ccccgctccg cttgcctcgc cgactctctc
      361 tctctctctc tctctctctc tctctctctc tctctctctc tctctctctc tctctgccac
      421 ccccccctcg ttcctctggc tggctcggct gaagcgagaa ggcagcagga gcacgcggcg
      481 gcggtggcgg tggtggtggc ggctgcacgg gcggagcgcg cagctcggcg agggagggag
      541 ggggctccgg cggtcgcgcg gccagggagg gcggcgaggc agggagggcc gcgagccagc
      601 tgaatgtggc cacaggagcc ctggagtccc agctccagcc ttggagaacg gggatgagct
      661 cgggctgggc ataaaagtca gggcagagcc atctattgct tacatttgct tctgggatcc
      721 agatctttcg aagctagcgc taccggtcgc caccatggtg agcaagggcg aggagctgtt
      781 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      841 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agctgatctg
      901 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctgg gctacggcgt
      961 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     1021 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     1081 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     1141 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     1201 caacgtctat atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg
     1261 ccacaacatc gaggacggcg gcgtgcagct cgccgaccac taccagcaga acacccccat
     1321 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccaagctgag
     1381 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     1441 gatcactctc ggcatggacg agctgtacaa gtaagtcgac ggcgcgccgc ggccgcgaat
     1501 tcgatatcat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     1561 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     1621 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttag ttcttgccac
     1681 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     1741 tgacaattcc gtggctcgag agatcttcga ctgtgccttc tagttgccag ccatctgttg
     1801 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     1861 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     1921 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gagatctcac
     1981 gtgcggaccg agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc
     2041 gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg
     2101 gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt
     2161 ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc
     2221 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     2281 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     2341 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     2401 tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc
     2461 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     2521 tgttccaaac tggaacaaca ctcaacccta tctcgggcta ttcttttgat ttataaggga
     2581 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     2641 attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg
     2701 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     2761 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     2821 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     2881 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     2941 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     3001 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     3061 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     3121 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     3181 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     3241 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     3301 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     3361 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     3421 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     3481 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     3541 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     3601 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     3661 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     3721 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     3781 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     3841 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     3901 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     3961 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     4021 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     4081 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     4141 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     4201 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     4261 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     4321 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     4381 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     4441 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     4501 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     4561 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     4621 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     4681 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt
//
