LOCUS       Exported                4470 bp ds-DNA     circular UNA 01-MAY-2024
DEFINITION  pAAV-AiE0675m-minBG-SYFP2-WPRE-BGHpA.
ACCESSION   Circular
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 12, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     LTR             complement(1..141)
                     /label=ITR
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     ITR"
                     /note="/Transferred_From=ITR"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=yoav.ben-simon"
     enhancer        152..391
                     /label=AiE0675m
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     AiE0675m "
                     /note="/Transferred_From=AiE0675m"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     AiE0675m "
                     /note="/X-Transferred_From=AiE0675m"
                     /note="/X-Transferred_Similarity=100.00%"
     promoter        395..447
                     /label=minBG promoter
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     minBG
                     promoter (Beta Globin minimal promoter)"
                     /note="/Transferred_From=Beta Globin minimal promoter"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=yoav.ben-simon"
                     /note="/modified_by=yoav.ben-simon"
     regulatory      482..491
                     /label=Kozak sequence
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     Kozak sequence"
                     /note="/Transferred_From=Kozak sequence"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     Kozak sequence"
                     /note="/X-Transferred_From=Kozak sequence"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/modified_by=yoav.ben-simon"
     CDS             488..1207
                     /label=SYFP2
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     SYFP2"
                     /note="/Transferred_From=SYFP2"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=yoav.ben-simon"
     3'UTR           1242..1487
                     /label=WPRE3
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     WPRE3"
                     /note="/Transferred_From=WPRE3"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=yoav.ben-simon"
                     /note="/modified_by=yoav.ben-simon"
     polyA_signal    1501..1704
                     /label=BGH-pA
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     BGH-pA"
                     /note="/Transferred_From=BGH-pA"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=yoav.ben-simon"
     LTR             1733..1873
                     /label=ITR
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     ITR"
                     /note="/Transferred_From=ITR"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=yoav.ben-simon"
     rep_origin      1965..2271
                     /label=f1 Origin
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; f1 
                     Origin"
                     /note="/Transferred_From=f1 Origin"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     f1 Origin"
                     /note="/X-Transferred_From=f1 Origin"
                     /note="/X-Transferred_Similarity=100.00%"
     promoter        2685..2789
                     /gene="bla"
                     /label=AmpR promoter
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     AmpR promoter"
                     /note="/Transferred_From=AmpR promoter"
                     /note="/Transferred_Similarity=100.00%"
     misc_feature    2720..2727
                     /label=FRT3 spacer (FASEBJ 2011, 25, 1)
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     FRT3 spacer (FASEBJ 2011, 25, 1)"
                     /note="/Transferred_From=FRT3 spacer (FASEBJ 2011, 25, 1)"
                     /note="/Transferred_Similarity=100.00%"
     CDS             2790..3647
                     /label=AmpR
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     AmpR"
                     /note="/Transferred_From=AmpR"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=yoav.ben-simon"
                     /note="/modified_by=yoav.ben-simon"
     rep_origin      3798..4465
                     /label=pUC ori
                     /label=pUC\ori
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     pUC\ori
                     (pUC ori)"
                     /note="/Transferred_From=pUC ori"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/vntifkey=33"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtaagca gagccccttt tgtcagcctt
      181 ctctcttact gttgtggttg tcatcacaac cccctccctc cagtaaaatg aagacacgga
      241 ttctccttgg ttactggggg aggtatccag gatacgccag gcctccagca ccagaataag
      301 acagccaagg cctccagcct tgtacactga agcagctcct ttcctctcta gaacaaagct
      361 agccattcct gaattaccca gaacgcgtga gctcgggctg ggcataaaag tcagggcaga
      421 gccatctatt gcttacattt gcttctggga tccagatctt tcgaagctag cgctaccggt
      481 cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
      541 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
      601 cacctacggc aagctgaccc tgaagctgat ctgcaccacc ggcaagctgc ccgtgccctg
      661 gcccaccctc gtgaccaccc tgggctacgg cgtgcagtgc ttcgcccgct accccgacca
      721 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
      781 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
      841 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
      901 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcaccg ccgacaagca
      961 gaagaacggc atcaaggcca acttcaagat ccgccacaac atcgaggacg gcggcgtgca
     1021 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     1081 caaccactac ctgagctacc agtccaagct gagcaaagac cccaacgaga agcgcgatca
     1141 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     1201 caagtaagtc gacggcgcgc cgcggccgcg aattcgatat cataatcaac ctctggatta
     1261 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     1321 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     1381 ctccttgtat aaatcctggt tagttcttgc cacggcggaa ctcatcgccg cctgccttgc
     1441 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggctc gagagatctt
     1501 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     1561 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     1621 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     1681 attgggaaga caatagcagg catgagatct cacgtgcgga ccgagcggcc gcaggaaccc
     1741 ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga
     1801 ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc
     1861 agctgcctgc aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     1921 caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg
     1981 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     2041 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     2101 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     2161 atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     2221 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     2281 ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     2341 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa
     2401 ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     2461 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     2521 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     2581 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     2641 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     2701 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     2761 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     2821 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     2881 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     2941 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     3001 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     3061 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     3121 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     3181 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     3241 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     3301 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     3361 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     3421 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     3481 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     3541 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     3601 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     3661 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     3721 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     3781 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     3841 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     3901 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     3961 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     4021 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     4081 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     4141 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     4201 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     4261 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     4321 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     4381 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     4441 tggccttttg ctggcctttt gctcacatgt
//
