LOCUS       Exported                5103 bp ds-DNA     circular UNA 01-MAY-2024
DEFINITION  pAAV-AiE0552h-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE-BGHpA.
ACCESSION   Circular
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5103)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 12, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5103
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     LTR             complement(1..130)
                     /label=ITR
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=92.20%; Adjusted-Interval-0:0/0; ITR"
                     /note="/Transferred_From=ITR"
                     /note="/Transferred_Similarity=92.20%"
                     /note="/created_by=yoav.ben-simon"
     enhancer        144..475
                     /label=AiE0552h
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     AiE0552h "
                     /note="/Transferred_From=AiE0552h"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     AiE0552h "
                     /note="/X-Transferred_From=AiE0552h"
                     /note="/X-Transferred_Similarity=100.00%"
     promoter        482..534
                     /label=minBG promoter
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     minBG
                     promoter (Beta Globin minimal promoter)"
                     /note="/Transferred_From=Beta Globin minimal promoter"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=yoav.ben-simon"
                     /note="/modified_by=yoav.ben-simon"
     regulatory      569..578
                     /label=Kozak sequence
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     Kozak sequence"
                     /note="/Transferred_From=Kozak sequence"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     Kozak sequence"
                     /note="/X-Transferred_From=Kozak sequence"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/modified_by=yoav.ben-simon"
     CDS             575..1294
                     /label=SYFP2
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=97.35%; Adjusted-Interval-0:0/-3;
                     Cerulean "
                     /note="/Alternative_Match=96.94%; Adjusted-Interval-0:0/0; 
                     EYFP"
                     /note="/Alternative_Match=96.81%; Adjusted-Interval-0:0/0; 
                     mCitrine "
                     /note="/Alternative_Match=96.54%; Adjusted-Interval-0:-3/0;
                     EGFP"
                     /note="/Alternative_Match=95.56%; Adjusted-Interval-0:0/0; 
                     mCerulean3"
                     /note="/Alternative_Match=93.30%; 
                     Adjusted-Interval-0:-44/-3; mTurquoise"
                     /note="/Primary_Match=98.75%; Adjusted-Interval-0:0/0; 
                     SYFP2"
                     /note="/Transferred_From=SYFP2"
                     /note="/Transferred_Similarity=98.75%"
                     /note="/created_by=yoav.ben-simon"
     misc_feature    1370..1435
                     /label=3X FLAG
                     /label=nonstandard type:  Tag
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=98.48%; Adjusted-Interval-0:0/0; 3X 
                     FLAG
                     (3XFLAG)"
                     /note="/Transferred_From=3XFLAG"
                     /note="/Transferred_Similarity=98.48%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     3X FLAG"
                     /note="/X-Transferred_From=3X FLAG"
                     /note="/X-Transferred_Similarity=100.00%"
     misc_feature    1436..1465
                     /label=10 a.a. linker
                     /label=nonstandard type:  Linker
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 10 
                     a.a. linker"
                     /note="/Transferred_From=10 a.a. linker"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     10 a.a. linker"
                     /note="/X-Transferred_From=10 a.a. linker"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/modified_by=yoav.ben-simon"
     sig_peptide     1466..1840
                     /label=H2B
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=98.93%; Adjusted-Interval-0:0/0; H2B"
                     /note="/Transferred_From=H2B"
                     /note="/Transferred_Similarity=98.93%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     H2B"
                     /note="/X-Transferred_From=H2B"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/modified_by=yoav.ben-simon"
     3'UTR           1875..2120
                     /label=WPRE3
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     WPRE3"
                     /note="/Transferred_From=WPRE3"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=yoav.ben-simon"
                     /note="/modified_by=yoav.ben-simon"
     polyA_signal    2134..2337
                     /label=BGH-pA
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     BGH-pA"
                     /note="/Transferred_From=BGH-pA"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=yoav.ben-simon"
     LTR             2366..2506
                     /label=ITR
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     ITR"
                     /note="/Transferred_From=ITR"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=yoav.ben-simon"
     rep_origin      2598..2904
                     /label=f1 Origin
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; f1 
                     Origin"
                     /note="/Transferred_From=f1 Origin"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     f1 Origin"
                     /note="/X-Transferred_From=f1 Origin"
                     /note="/X-Transferred_Similarity=100.00%"
     promoter        3318..3422
                     /gene="bla"
                     /label=AmpR promoter
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     AmpR promoter"
                     /note="/Transferred_From=AmpR promoter"
                     /note="/Transferred_Similarity=100.00%"
     misc_feature    3353..3360
                     /label=FRT3 spacer (FASEBJ 2011, 25, 1)
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     FRT3 spacer (FASEBJ 2011, 25, 1)"
                     /note="/Transferred_From=FRT3 spacer (FASEBJ 2011, 25, 1)"
                     /note="/Transferred_Similarity=100.00%"
     CDS             3423..4280
                     /label=AmpR
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     AmpR"
                     /note="/Transferred_From=AmpR"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=yoav.ben-simon"
                     /note="/modified_by=yoav.ben-simon"
     rep_origin      4431..5098
                     /label=pUC ori
                     /label=pUC\ori
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     pUC\ori
                     (pUC ori)"
                     /note="/Transferred_From=pUC ori"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/vntifkey=33"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtaagcca tgttattgca agagcactga gagacaaact
      181 tttaaaaaag cagatcgcat ggcaagtcca taaaatcagg attacaaagg catctggaca
      241 cagacactat gaattctaga tctgaacggg aatagttaag ttcgggaaga gacaattgtc
      301 agaattcatg ggcttagaac agtggcctgc acagctccct ggcacgtggc tagatgaagc
      361 tggattaaaa ataaagatca acccaaacct atagcaaacc agactaaaca tatggacaga
      421 aattttatgg aaaaagaagt aaaaatatct cttagtcata tgaaaatata tttcagagct
      481 cgggctgggc ataaaagtca gggcagagcc atctattgct tacatttgct tctgggatcc
      541 agatctttcg aagctagcgc taccggtcgc caccatggtc tccaagggcg aggagctgtt
      601 caccggggtg gtgcccatcc tggtcgagct ggacggcgat gtcaacggcc acaagttcag
      661 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agctgatctg
      721 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctgg gctacggcgt
      781 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      841 gcccgaaggc tacgtccagg agcgcaccat cttcttcaaa gacgacggca actacaagac
      901 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
      961 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     1021 caacgtctat atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg
     1081 ccacaacatc gaggacggcg gcgtgcagct cgccgaccac taccagcaga acacccccat
     1141 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccaagctgag
     1201 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     1261 gatcactctc ggcatggacg agctgtacaa gggctctggt gctaccaact tctcactgtt
     1321 gaaacaggca ggggatgtag aggagaatcc agggcctggt gctagtggag actacaaaga
     1381 ccatgacgga gattataaag atcatgacat cgattacaag gatgacgatg acaagtccgg
     1441 actcagatct ggaggctccg gaggcccaga gccagcgaag tctgctcccg ccccgaaaaa
     1501 gggctccaag aaggcggtga ctaaggcgca gaagaaaggc ggcaagaagc gcaagcgcag
     1561 ccgcaaggag agctattcca tctatgtgta caaggttctg aagcaggtcc accctgacac
     1621 cggcatttcg tccaaggcca tgggcatcat gaattcgttt gtgaacgaca ttttcgagcg
     1681 catcgcagga gaggcttccc gcctggcgca ttacaacaag cgctcgacca tcacctcccg
     1741 ggagatccag acggccgtgc gcctgctgct gcctggggag ttggccaagc acgccgtgtc
     1801 cgagggtact aaggccatca ccaagtacac cagcgctaag taatgaggcg cgccgcggcc
     1861 gcgaattcga tatcataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     1921 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     1981 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttagttct
     2041 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     2101 gggcactgac aattccgtgg ctcgagagat cttcgactgt gccttctagt tgccagccat
     2161 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     2221 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     2281 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgaga
     2341 tctcacgtgc ggaccgagcg gccgcaggaa cccctagtga tggagttggc cactccctct
     2401 ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt
     2461 gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg
     2521 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt
     2581 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     2641 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     2701 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     2761 gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc
     2821 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     2881 gactcttgtt ccaaactgga acaacactca accctatctc gggctattct tttgatttat
     2941 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     3001 acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct
     3061 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     3121 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     3181 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
     3241 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     3301 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     3361 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     3421 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     3481 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     3541 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     3601 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     3661 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     3721 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     3781 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     3841 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     3901 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     3961 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     4021 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     4081 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     4141 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     4201 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     4261 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     4321 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     4381 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     4441 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     4501 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     4561 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     4621 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     4681 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     4741 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     4801 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     4861 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     4921 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     4981 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     5041 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     5101 tgt
//
