LOCUS       Exported                5100 bp ds-DNA     circular UNA 04-JUN-2024
DEFINITION  pAAV-AiE0459m-minBG-iCre(R297T)-BGHpA.
ACCESSION   Circular
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 12, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5100
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     LTR             complement(1..141)
                     /label=ITR
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     ITR"
                     /note="/Transferred_From=ITR"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=yoav.ben-simon"
     enhancer        174..960
                     /label=AiE0459m
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=99.81%; Adjusted-Interval-0:0/0; 
                     AiE0459m "
                     /note="/Transferred_From=AiE0459m"
                     /note="/Transferred_Similarity=99.81%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     AiE0459m "
                     /note="/X-Transferred_From=AiE0459m"
                     /note="/X-Transferred_Similarity=100.00%"
     promoter        967..1019
                     /label=minBG promoter
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     minBG
                     promoter (Beta Globin minimal promoter)"
                     /note="/Transferred_From=Beta Globin minimal promoter"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=yoav.ben-simon"
                     /note="/modified_by=yoav.ben-simon"
     regulatory      1042..1051
                     /label=Kozak sequence
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     Kozak sequence"
                     /note="/Transferred_From=Kozak sequence"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     Kozak sequence"
                     /note="/X-Transferred_From=Kozak sequence"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/modified_by=yoav.ben-simon"
     regulatory      complement(1046..1055)
                     /label=Kozak sequence
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=90.00%; Adjusted-Interval-0:0/0; 
                     Kozak sequence"
                     /note="/Transferred_From=Kozak sequence"
                     /note="/Transferred_Similarity=90.00%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     Kozak sequence"
                     /note="/X-Transferred_From=Kozak sequence"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/modified_by=yoav.ben-simon"
     CDS             1048..2103
                     /codon_start=1
                     /label=iCre(R297T)
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     iCre(297T)"
                     /note="/Transferred_From=iCre(297T)"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/modified_by=yoav.ben-simon"
                     /note="/vntifkey=21"
                     /translation="MVPKKKRKVSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAF
                     SEHTWKMLLSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLN
                     MLHRRSGLPRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRC
                     QDIRNLAFLGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALS
                     LGVTKLVERWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIY
                     GAKDDSGQRYLAWSGHSARVGAATDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSET
                     GAMVRLLEDGD"
     polyA_signal    2137..2340
                     /label=BGH-pA
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     BGH-pA"
                     /note="/Transferred_From=BGH-pA"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=yoav.ben-simon"
     LTR             2363..2503
                     /label=ITR
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     ITR"
                     /note="/Transferred_From=ITR"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=yoav.ben-simon"
     rep_origin      2595..2901
                     /label=f1 Origin
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; f1 
                     Origin"
                     /note="/Transferred_From=f1 Origin"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     f1 Origin"
                     /note="/X-Transferred_From=f1 Origin"
                     /note="/X-Transferred_Similarity=100.00%"
     promoter        3315..3419
                     /gene="bla"
                     /label=AmpR promoter
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     AmpR promoter"
                     /note="/Transferred_From=AmpR promoter"
                     /note="/Transferred_Similarity=100.00%"
     misc_feature    3350..3357
                     /label=FRT3 spacer (FASEBJ 2011, 25, 1)
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     FRT3 spacer (FASEBJ 2011, 25, 1)"
                     /note="/Transferred_From=FRT3 spacer (FASEBJ 2011, 25, 1)"
                     /note="/Transferred_Similarity=100.00%"
     CDS             3420..4277
                     /codon_start=1
                     /label=AmpR
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     AmpR"
                     /note="/Transferred_From=AmpR"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=yoav.ben-simon"
                     /note="/modified_by=yoav.ben-simon"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4428..5095
                     /label=pUC ori
                     /label=pUC\ori
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     pUC\ori
                     (pUC ori)"
                     /note="/Transferred_From=pUC ori"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/vntifkey=33"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgttcngc ggccgcacgc ggtngactgt
      181 gtcatgactc tcagtgtctc ttttttccac ggttctggga tcatcatttc tttctcttca
      241 gacagtctag atgagcagcc gagtgcttat ctttaaggct gactctagac agccagttgt
      301 tatttcataa atctgttaaa actcaccgtg caaacaccag cgttcnttag ctgaccatcc
      361 tcaagcacac gtggagtgtg acctctgctt ccttctctca tttcacatat tagtaatgtg
      421 cgtctatgaa tagttacaag tgagaaagag aaatggagat tttaccattt aatcatctcc
      481 gaagagaagg tgagaagaaa ttgagaaagg taatttgcat accatgcata ggcattagag
      541 aaagcatttt ttttcttaac tctccatttg tttacagata tttccatatg gccttttaaa
      601 aacttcaaca gagaaattcg aacacaacta actggaattt caattttcat ataaaaccag
      661 catcatagat atatatgtag ctactctccc acttcacatt attcagttag tatattttct
      721 aagctaattt ccaatccatt ttaagactat gtacagctca tatgaggccc ccatatgttc
      781 gttcacgtta ctttgcatcc gtttacatga ttaaacccca agaacaaatc gattagaagc
      841 caagagcaaa tgcttttggc tccacattag aaaactcaac agagaaacct ttacaagtga
      901 tagtcatctg ggtaagagac atgcaaacat aattaccatt attcgtggac ttaattccct
      961 gagctcgggc tgggcataaa agtcagggca gagccatcta ttgcttacat ttgcttctgg
     1021 gatccagatc tttccaagct agccaccatg gtgcccaaga agaagaggaa agtctccaac
     1081 ctgctgactg tgcaccaaaa cctgcctgcc ctccctgtgg atgccacctc tgatgaagtc
     1141 aggaagaacc tgatggacat gttcagggac aggcaggcct tctctgaaca cacctggaag
     1201 atgctcctgt ctgtgtgcag atcctgggct gcctggtgca agctgaacaa caggaaatgg
     1261 ttccctgctg aacctgagga tgtgagggac tacctcctgt acctgcaagc cagaggcctg
     1321 gctgtgaaga ccatccaaca gcacctgggc cagctcaaca tgctgcacag gagatctggc
     1381 ctgcctcgcc cttctgactc caatgctgtg tccctggtga tgaggagaat cagaaaggag
     1441 aatgtggatg ctggggagag agccaagcag gccctggcct ttgaacgcac tgactttgac
     1501 caagtcagat ccctgatgga gaactctgac agatgccagg acatcaggaa cctggccttc
     1561 ctgggcattg cctacaacac cctgctgcgc attgccgaaa ttgccagaat cagagtgaag
     1621 gacatctccc gcaccgatgg tgggagaatg ctgatccaca ttggcaggac caagaccctg
     1681 gtgtccacag ctggtgtgga gaaggccctg tccctggggg ttaccaagct ggtggagaga
     1741 tggatctctg tgtctggtgt ggctgatgac cccaacaact acctgttctg ccgggtcaga
     1801 aagaatggtg tggctgcccc ttctgccacc tcccaactgt ccacccgggc cctggaaggg
     1861 atctttgagg ccacccaccg cctgatctat ggtgccaagg atgactctgg gcagagatac
     1921 ctggcctggt ctggccactc tgccagagtg ggtgctgcca ccgacatggc cagggctggt
     1981 gtgtccatcc ctgaaatcat gcaggctggt ggctggacca atgtgaacat tgtgatgaac
     2041 tacatcagaa acctggactc tgagactggg gccatggtga ggctgctcga agatggggac
     2101 tgaggcgcgc cgaattcaag cttctcgaga gatcttcgac tgtgccttct agttgccagc
     2161 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     2221 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     2281 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     2341 cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg
     2401 cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc
     2461 cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat
     2521 tttctcctta cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg
     2581 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     2641 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     2701 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     2761 ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat
     2821 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     2881 tcttgttcca aactggaaca acactcaacc ctatctcggg ctattctttt gatttataag
     2941 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     3001 cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct
     3061 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     3121 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     3181 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     3241 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     3301 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     3361 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     3421 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     3481 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     3541 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     3601 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     3661 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     3721 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     3781 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     3841 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     3901 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     3961 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     4021 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     4081 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     4141 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     4201 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     4261 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     4321 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     4381 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     4441 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     4501 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     4561 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     4621 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     4681 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     4741 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     4801 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     4861 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     4921 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     4981 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     5041 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
//
