LOCUS       Exported                5473 bp ds-DNA     circular UNA 01-MAY-2024
DEFINITION  pAAV-AiE2118m_3xC2-minBG-SYFP2-WPRE-BGHpA.
ACCESSION   Circular
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5473)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 12, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5473
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     LTR             complement(1..141)
                     /label=ITR
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     ITR"
                     /note="/Transferred_From=ITR"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=yoav.ben-simon"
     misc_feature    156..1397
                     /label=AiE2118m_3xC2
                     /label=nonstandard type:  Bashed_enhancer
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     AiE2118m_3x C2"
                     /note="/Transferred_From=AiE2118m_3xC2"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     AiE2118m_3x C2"
                     /note="/X-Transferred_From=AiE2118m_3xC2"
                     /note="/X-Transferred_Similarity=100.00%"
     promoter        1404..1456
                     /label=minBG promoter
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     minBG
                     promoter (Beta Globin minimal promoter)"
                     /note="/Transferred_From=Beta Globin minimal promoter"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=yoav.ben-simon"
                     /note="/modified_by=yoav.ben-simon"
     regulatory      1491..1500
                     /label=Kozak sequence
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     Kozak sequence"
                     /note="/Transferred_From=Kozak sequence"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     Kozak sequence"
                     /note="/X-Transferred_From=Kozak sequence"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/modified_by=yoav.ben-simon"
     CDS             1497..2216
                     /label=SYFP2
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     SYFP2"
                     /note="/Transferred_From=SYFP2"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=yoav.ben-simon"
     3'UTR           2251..2496
                     /label=WPRE3
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     WPRE3"
                     /note="/Transferred_From=WPRE3"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=yoav.ben-simon"
                     /note="/modified_by=yoav.ben-simon"
     polyA_signal    2510..2713
                     /label=BGH-pA
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     BGH-pA"
                     /note="/Transferred_From=BGH-pA"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=yoav.ben-simon"
     LTR             2736..2876
                     /label=ITR
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     ITR"
                     /note="/Transferred_From=ITR"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=yoav.ben-simon"
     rep_origin      2968..3274
                     /label=f1 Origin
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; f1 
                     Origin"
                     /note="/Transferred_From=f1 Origin"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     f1 Origin"
                     /note="/X-Transferred_From=f1 Origin"
                     /note="/X-Transferred_Similarity=100.00%"
     promoter        3688..3792
                     /gene="bla"
                     /label=AmpR promoter
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     AmpR promoter"
                     /note="/Transferred_From=AmpR promoter"
                     /note="/Transferred_Similarity=100.00%"
     misc_feature    3723..3730
                     /label=FRT3 spacer (FASEBJ 2011, 25, 1)
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     FRT3 spacer (FASEBJ 2011, 25, 1)"
                     /note="/Transferred_From=FRT3 spacer (FASEBJ 2011, 25, 1)"
                     /note="/Transferred_Similarity=100.00%"
     CDS             3793..4650
                     /label=AmpR
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     AmpR"
                     /note="/Transferred_From=AmpR"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=yoav.ben-simon"
                     /note="/modified_by=yoav.ben-simon"
     rep_origin      4801..5468
                     /label=pUC ori
                     /label=pUC\ori
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     pUC\ori
                     (pUC ori)"
                     /note="/Transferred_From=pUC ori"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/vntifkey=33"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgttgtga atctggatgc gtattgaaag
      181 aaacattagt ccttttgtca gcttggcaag tctattgttc tgagccaggc ccaaccaatt
      241 aacatctttt gccaatccct gtcagcaggg gcttgcaagg aggagagaaa ggggggccgg
      301 tcagatgcta atttagatac aattgtgtca gggtgcatgg gaggggtaac tctgaagaag
      361 agactccagt ttaatgaagc aaacagcttg ggtcccagtg accctgcagg ggtaatttta
      421 aaaggcccct ctgtgttcct gtgccaaatt gcagagggcc agcaacaaga agcccttcaa
      481 aaaaaaaaaa agagggagca ggataatcat ccacttaatc tgctcattaa acaaatctct
      541 caggcggact taacgtgaat tagcacttct gtgaatctgg atgcgtattg aaagaaacat
      601 tagtcctttt gtcagcttgg caagtctatt gttctgagcc aggcccaacc aattaacatc
      661 ttttgccaat ccctgtcagc aggggcttgc aaggaggaga gaaagggggg ccggtcagat
      721 gctaatttag atacaattgt gtcagggtgc atgggagggg taactctgaa gaagagactc
      781 cagtttaatg aagcaaacag cttgggtccc agtgaccctg caggggtaat tttaaaaggc
      841 ccctctgtgt tcctgtgcca aattgcagag ggccagcaac aagaagccct tcaaaaaaaa
      901 aaaaagaggg agcaggataa tcatccactt aatctgctca ttaaacaaat ctctcaggcg
      961 gacttaacgt gaattagcac ttctgtgaat ctggatgcgt attgaaagaa acattagtcc
     1021 ttttgtcagc ttggcaagtc tattgttctg agccaggccc aaccaattaa catcttttgc
     1081 caatccctgt cagcaggggc ttgcaaggag gagagaaagg ggggccggtc agatgctaat
     1141 ttagatacaa ttgtgtcagg gtgcatggga ggggtaactc tgaagaagag actccagttt
     1201 aatgaagcaa acagcttggg tcccagtgac cctgcagggg taattttaaa aggcccctct
     1261 gtgttcctgt gccaaattgc agagggccag caacaagaag cccttcaaaa aaaaaaaaag
     1321 agggagcagg ataatcatcc acttaatctg ctcattaaac aaatctctca ggcggactta
     1381 acgtgaatta gcacttcgag ctcgggctgg gcataaaagt cagggcagag ccatctattg
     1441 cttacatttg cttctgggat ccagatcttt cgaagctagc gctaccggtc gccaccatgg
     1501 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     1561 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     1621 agctgaccct gaagctgatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     1681 tgaccaccct gggctacggc gtgcagtgct tcgcccgcta ccccgaccac atgaagcagc
     1741 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     1801 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     1861 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     1921 tggagtacaa ctacaacagc cacaacgtct atatcaccgc cgacaagcag aagaacggca
     1981 tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cggcgtgcag ctcgccgacc
     2041 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     2101 tgagctacca gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     2161 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaagtcg
     2221 acggcgcgcc gcggccgcga attcgatatc ataatcaacc tctggattac aaaatttgtg
     2281 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     2341 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     2401 aatcctggtt agttcttgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     2461 caggggctcg gctgttgggc actgacaatt ccgtggctcg agagatcttc gactgtgcct
     2521 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     2581 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     2641 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     2701 aatagcaggc atgcacgtgc ggaccgagcg gccgcaggaa cccctagtga tggagttggc
     2761 cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg
     2821 cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg
     2881 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag
     2941 caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     3001 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     3061 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     3121 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca
     3181 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     3241 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc gggctattct
     3301 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     3361 caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc
     3421 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     3481 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     3541 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
     3601 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     3661 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     3721 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     3781 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     3841 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     3901 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     3961 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     4021 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     4081 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     4141 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     4201 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     4261 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     4321 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     4381 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     4441 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     4501 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     4561 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     4621 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     4681 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     4741 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     4801 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     4861 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     4921 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     4981 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     5041 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     5101 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     5161 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     5221 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     5281 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     5341 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     5401 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     5461 tttgctcaca tgt
//
