LOCUS       Exported                5380 bp ds-DNA     circular UNA 01-MAY-2024
DEFINITION  pAAV-AiE2122_3xC2-minBG-SYFP2-WPRE-BGHpA.
ACCESSION   Circular
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5380)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 12, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5380
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     LTR             complement(1..141)
                     /label=ITR
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     ITR"
                     /note="/Transferred_From=ITR"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=yoav.ben-simon"
     misc_feature    156..1304
                     /label=AiE2112m_3xC2
                     /label=nonstandard type:  Bashed_enhancer
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; Adjusted-Interval-0:0/0;
                     AiE2112m_3x C2"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0;
                     AiE2112m_3xC2 (AiE2122m_3x C2)"
                     /note="/Transferred_From=AiE2122m_3xC2"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Alternative_Match=100.00%; 
                     Adjusted-Interval-0:0/0; AiE2122m_3x C2"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     AiE2112m_3x C2"
                     /note="/X-Transferred_From=AiE2112m_3xC2"
                     /note="/X-Transferred_Similarity=100.00%"
     promoter        1311..1363
                     /label=minBG promoter
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     minBG
                     promoter (Beta Globin minimal promoter)"
                     /note="/Transferred_From=Beta Globin minimal promoter"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=yoav.ben-simon"
                     /note="/modified_by=yoav.ben-simon"
     regulatory      1398..1407
                     /label=Kozak sequence
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     Kozak sequence"
                     /note="/Transferred_From=Kozak sequence"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     Kozak sequence"
                     /note="/X-Transferred_From=Kozak sequence"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/modified_by=yoav.ben-simon"
     CDS             1404..2123
                     /label=SYFP2
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     SYFP2"
                     /note="/Transferred_From=SYFP2"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=yoav.ben-simon"
     3'UTR           2158..2403
                     /label=WPRE3
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     WPRE3"
                     /note="/Transferred_From=WPRE3"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=yoav.ben-simon"
                     /note="/modified_by=yoav.ben-simon"
     polyA_signal    2417..2620
                     /label=BGH-pA
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     BGH-pA"
                     /note="/Transferred_From=BGH-pA"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=yoav.ben-simon"
     LTR             2643..2783
                     /label=ITR
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     ITR"
                     /note="/Transferred_From=ITR"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=yoav.ben-simon"
     rep_origin      2875..3181
                     /label=f1 Origin
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; f1 
                     Origin"
                     /note="/Transferred_From=f1 Origin"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     f1 Origin"
                     /note="/X-Transferred_From=f1 Origin"
                     /note="/X-Transferred_Similarity=100.00%"
     promoter        3595..3699
                     /gene="bla"
                     /label=AmpR promoter
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     AmpR promoter"
                     /note="/Transferred_From=AmpR promoter"
                     /note="/Transferred_Similarity=100.00%"
     misc_feature    3630..3637
                     /label=FRT3 spacer (FASEBJ 2011, 25, 1)
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     FRT3 spacer (FASEBJ 2011, 25, 1)"
                     /note="/Transferred_From=FRT3 spacer (FASEBJ 2011, 25, 1)"
                     /note="/Transferred_Similarity=100.00%"
     CDS             3700..4557
                     /label=AmpR
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     AmpR"
                     /note="/Transferred_From=AmpR"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=yoav.ben-simon"
                     /note="/modified_by=yoav.ben-simon"
     rep_origin      4708..5375
                     /label=pUC ori
                     /label=pUC\ori
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     pUC\ori
                     (pUC ori)"
                     /note="/Transferred_From=pUC ori"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/vntifkey=33"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgttcctc tgtcccactg gctgtggctc
      181 agtgtttatt tattggctgt ttagtatgga gaaacatcca gtctttcaac attgacaaca
      241 aatccaaata atgagcctgc ccatcacgct acactgggta tcatctttgc ctgttaggct
      301 cttggctggt tttttgctgt atttgactat cctcaaagtt tgggagttct gactgcgggt
      361 tgttattttc caattcttat taacacttgc caaatctttt gtcatgcatc tttatactca
      421 tgacagcttc taggtcaaat tttagttttg atcactggat ctttagaagg aaagctaagg
      481 aactgacaga catagccagc tccttttttt atgctggcaa tggatactga ttagctgttc
      541 ctctgtccca ctggctgtgg ctcagtgttt atttattggc tgtttagtat ggagaaacat
      601 ccagtctttc aacattgaca acaaatccaa ataatgagcc tgcccatcac gctacactgg
      661 gtatcatctt tgcctgttag gctcttggct ggttttttgc tgtatttgac tatcctcaaa
      721 gtttgggagt tctgactgcg ggttgttatt ttccaattct tattaacact tgccaaatct
      781 tttgtcatgc atctttatac tcatgacagc ttctaggtca aattttagtt ttgatcactg
      841 gatctttaga aggaaagcta aggaactgac agacatagcc agctcctttt tttatgctgg
      901 caatggatac tgattagctg ttcctctgtc ccactggctg tggctcagtg tttatttatt
      961 ggctgtttag tatggagaaa catccagtct ttcaacattg acaacaaatc caaataatga
     1021 gcctgcccat cacgctacac tgggtatcat ctttgcctgt taggctcttg gctggttttt
     1081 tgctgtattt gactatcctc aaagtttggg agttctgact gcgggttgtt attttccaat
     1141 tcttattaac acttgccaaa tcttttgtca tgcatcttta tactcatgac agcttctagg
     1201 tcaaatttta gttttgatca ctggatcttt agaaggaaag ctaaggaact gacagacata
     1261 gccagctcct ttttttatgc tggcaatgga tactgattag ctgtgagctc gggctgggca
     1321 taaaagtcag ggcagagcca tctattgctt acatttgctt ctgggatcca gatctttcga
     1381 agctagcgct accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg
     1441 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     1501 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gctgatctgc accaccggca
     1561 agctgcccgt gccctggccc accctcgtga ccaccctggg ctacggcgtg cagtgcttcg
     1621 cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     1681 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     1741 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     1801 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     1861 tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc cacaacatcg
     1921 aggacggcgg cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     1981 ccgtgctgct gcccgacaac cactacctga gctaccagtc caagctgagc aaagacccca
     2041 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     2101 gcatggacga gctgtacaag taagtcgacg gcgcgccgcg gccgcgaatt cgatatcata
     2161 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     2221 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     2281 tggctttcat tttctcctcc ttgtataaat cctggttagt tcttgccacg gcggaactca
     2341 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     2401 tggctcgaga gatcttcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     2461 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     2521 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     2581 gacagcaagg gggaggattg ggaagacaat agcaggcatg cacgtgcgga ccgagcggcc
     2641 gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga
     2701 ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga
     2761 gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat tttctcctta cgcatctgtg
     2821 cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta
     2881 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     2941 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     3001 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     3061 aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     3121 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     3181 acactcaacc ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggcc
     3241 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     3301 acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     3361 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     3421 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     3481 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     3541 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     3601 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     3661 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     3721 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     3781 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     3841 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     3901 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     3961 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     4021 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     4081 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     4141 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     4201 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     4261 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     4321 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     4381 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     4441 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     4501 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     4561 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     4621 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     4681 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     4741 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     4801 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     4861 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     4921 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     4981 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     5041 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     5101 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     5161 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     5221 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     5281 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     5341 ttacggttcc tggccttttg ctggcctttt gctcacatgt
//
