LOCUS       Exported                5311 bp ds-DNA     circular UNA 01-MAY-2024
DEFINITION  pAAV-AiE1037m-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE-BGHpA.
ACCESSION   Circular
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5311)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 12, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5311
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     LTR             complement(1..130)
                     /label=ITR
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=92.20%; Adjusted-Interval-0:0/0; ITR"
                     /note="/Transferred_From=ITR"
                     /note="/Transferred_Similarity=92.20%"
                     /note="/created_by=yoav.ben-simon"
     enhancer        145..683
                     /label=AiE1037m
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     AiE1037m"
                     /note="/Transferred_From=AiE1037m"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     AiE1037m"
                     /note="/X-Transferred_From=AiE1037m"
                     /note="/X-Transferred_Similarity=100.00%"
     promoter        690..742
                     /label=minBG promoter
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     minBG
                     promoter (Beta Globin minimal promoter)"
                     /note="/Transferred_From=Beta Globin minimal promoter"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=yoav.ben-simon"
                     /note="/modified_by=yoav.ben-simon"
     regulatory      777..786
                     /label=Kozak sequence
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     Kozak sequence"
                     /note="/Transferred_From=Kozak sequence"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     Kozak sequence"
                     /note="/X-Transferred_From=Kozak sequence"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/modified_by=yoav.ben-simon"
     CDS             783..1502
                     /label=SYFP2
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=97.35%; Adjusted-Interval-0:0/-3;
                     Cerulean "
                     /note="/Alternative_Match=96.94%; Adjusted-Interval-0:0/0; 
                     EYFP"
                     /note="/Alternative_Match=96.81%; Adjusted-Interval-0:0/0; 
                     mCitrine "
                     /note="/Alternative_Match=96.54%; Adjusted-Interval-0:-3/0;
                     EGFP"
                     /note="/Alternative_Match=95.56%; Adjusted-Interval-0:0/0; 
                     mCerulean3"
                     /note="/Alternative_Match=93.30%; 
                     Adjusted-Interval-0:-44/-3; mTurquoise"
                     /note="/Primary_Match=98.75%; Adjusted-Interval-0:0/0; 
                     SYFP2"
                     /note="/Transferred_From=SYFP2"
                     /note="/Transferred_Similarity=98.75%"
                     /note="/created_by=yoav.ben-simon"
     misc_feature    1578..1643
                     /label=3X FLAG
                     /label=nonstandard type:  Tag
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=98.48%; Adjusted-Interval-0:0/0; 3X 
                     FLAG
                     (3XFLAG)"
                     /note="/Transferred_From=3XFLAG"
                     /note="/Transferred_Similarity=98.48%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     3X FLAG"
                     /note="/X-Transferred_From=3X FLAG"
                     /note="/X-Transferred_Similarity=100.00%"
     misc_feature    1644..1673
                     /label=10 a.a. linker
                     /label=nonstandard type:  Linker
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 10 
                     a.a. linker"
                     /note="/Transferred_From=10 a.a. linker"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     10 a.a. linker"
                     /note="/X-Transferred_From=10 a.a. linker"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/modified_by=yoav.ben-simon"
     sig_peptide     1674..2048
                     /label=H2B
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     H2B"
                     /note="/Transferred_From=H2B"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     H2B"
                     /note="/X-Transferred_From=H2B"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/modified_by=yoav.ben-simon"
     3'UTR           2083..2328
                     /label=WPRE3
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     WPRE3"
                     /note="/Transferred_From=WPRE3"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=yoav.ben-simon"
                     /note="/modified_by=yoav.ben-simon"
     polyA_signal    2342..2545
                     /label=BGH-pA
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     BGH-pA"
                     /note="/Transferred_From=BGH-pA"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=yoav.ben-simon"
     LTR             2574..2714
                     /label=ITR
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     ITR"
                     /note="/Transferred_From=ITR"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=yoav.ben-simon"
     rep_origin      2806..3112
                     /label=f1 Origin
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; f1 
                     Origin"
                     /note="/Transferred_From=f1 Origin"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     f1 Origin"
                     /note="/X-Transferred_From=f1 Origin"
                     /note="/X-Transferred_Similarity=100.00%"
     promoter        3526..3630
                     /gene="bla"
                     /label=AmpR promoter
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     AmpR promoter"
                     /note="/Transferred_From=AmpR promoter"
                     /note="/Transferred_Similarity=100.00%"
     misc_feature    3561..3568
                     /label=FRT3 spacer (FASEBJ 2011, 25, 1)
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     FRT3 spacer (FASEBJ 2011, 25, 1)"
                     /note="/Transferred_From=FRT3 spacer (FASEBJ 2011, 25, 1)"
                     /note="/Transferred_Similarity=100.00%"
     CDS             3631..4488
                     /label=AmpR
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     AmpR"
                     /note="/Transferred_From=AmpR"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=yoav.ben-simon"
                     /note="/modified_by=yoav.ben-simon"
     rep_origin      4639..5306
                     /label=pUC ori
                     /label=pUC\ori
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     pUC\ori
                     (pUC ori)"
                     /note="/Transferred_From=pUC ori"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/vntifkey=33"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgttcttct gcagaatggg atgtttcagt cagggaggaa
      181 actgttactc aacaaaaggg ctgtaaataa tttgaatact tactgatttt tataatgcac
      241 tttgtagcac agggcttctt gaatataccc ttgagaaact ttctgggaat caatgacaca
      301 tcctttagtg catattttta aaagtggtgc tattttgaag actacactat gctaaggaca
      361 taaatgatta ttcattagaa tttcccacct tgggagaatt attggccagg tcccaaagtg
      421 tatgatcgtc actggagact gttttcatcc ttttcaagct gttcattgag ttgctttgat
      481 ccttttaaac ataaagttct aaagttaaat ttagtacacc tagggaaaag gctccttggt
      541 gtaaagcaaa aagctgctag aaagtgaaaa aaacgtcgtt tccccaaagg ctttgaatta
      601 aaattcaatt caatttcatt aacactcact gaatgcctgt agggggcaga acactgtgct
      661 aggcattaga actcgaaata tacgagctcg ggctgggcat aaaagtcagg gcagagccat
      721 ctattgctta catttgcttc tgggatccag atctttcgaa gctagcgcta ccggtcgcca
      781 ccatggtctc caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
      841 acggcgatgt caacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
      901 acggcaagct gaccctgaag ctgatctgca ccaccggcaa gctgcccgtg ccctggccca
      961 ccctcgtgac caccctgggc tacggcgtgc agtgcttcgc ccgctacccc gaccacatga
     1021 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     1081 tcttcaaaga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     1141 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     1201 acaagctgga gtacaactac aacagccaca acgtctatat caccgccgac aagcagaaga
     1261 acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcggc gtgcagctcg
     1321 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     1381 actacctgag ctaccagtcc aagctgagca aagaccccaa cgagaagcgc gatcacatgg
     1441 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg
     1501 gctctggtgc taccaacttc tcactgttga aacaggcagg ggatgtagag gagaatccag
     1561 ggcctggtgc tagtggagac tacaaagacc atgacggaga ttataaagat catgacatcg
     1621 attacaagga tgacgatgac aagtccggac tcagatctgg aggctccgga ggcccagagc
     1681 cagcgaagtc tgctcccgcc ccgaaaaagg gctccaagaa ggcggtgact aaggcgcaga
     1741 agaaaggcgg caagaagcgc aagcgcagcc gcaaggagag ctattccatc tatgtgtata
     1801 aggttctgaa gcaggtccac cctgacaccg gcatttcgtc caaggccatg ggcatcatga
     1861 actcgtttgt gaacgacatt ttcgagcgca tcgcaggtga ggcttcccgc ctggcgcatt
     1921 acaacaagcg ctcgaccatc acctccaggg agatccagac ggccgtgcgc ctgctgctgc
     1981 ctggggagtt ggccaagcac gccgtgtccg agggtactaa ggccatcacc aagtacacca
     2041 gcgctaagta atgaggcgcg ccgcggccgc gaattcgata tcataatcaa cctctggatt
     2101 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     2161 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     2221 cctccttgta taaatcctgg ttagttcttg ccacggcgga actcatcgcc gcctgccttg
     2281 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggct cgagagatct
     2341 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     2401 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     2461 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     2521 gattgggaag acaatagcag gcatgagatc tcacgtgcgg accgagcggc cgcaggaacc
     2581 cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg
     2641 accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg
     2701 cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     2761 acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg
     2821 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     2881 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     2941 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     3001 gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     3061 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     3121 cctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     3181 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca
     3241 attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     3301 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     3361 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     3421 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     3481 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     3541 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     3601 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     3661 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     3721 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     3781 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     3841 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     3901 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     3961 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     4021 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     4081 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     4141 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     4201 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     4261 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     4321 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     4381 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     4441 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     4501 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     4561 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     4621 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     4681 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     4741 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     4801 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     4861 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     4921 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     4981 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     5041 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     5101 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     5161 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     5221 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     5281 ctggcctttt gctggccttt tgctcacatg t
//
