LOCUS       Exported                5759 bp ds-DNA     circular UNA 01-MAY-2024
DEFINITION  pAAV-AiE0050m_3xC2-minBG-tdTomato-WPRE-BGHpA.
ACCESSION   Circular
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 12, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5759
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     LTR             complement(1..141)
                     /label=ITR
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     ITR"
                     /note="/Transferred_From=ITR"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=yoav.ben-simon"
     misc_feature    156..980
                     /label=AiE0050m_3xC2
                     /label=nonstandard type:  Bashed_enhancer
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Alternative_Match=100.00%; 
                     Adjusted-Interval-0:113/-571;
                     AiE0040m_C3"
                     /note="/Alternative_Match=100.00%; 
                     Adjusted-Interval-0:388/-296;
                     AiE0040m_C3"
                     /note="/Alternative_Match=100.00%; 
                     Adjusted-Interval-0:663/-21;
                     AiE0040m_C3"
                     /note="/Alternative_Match=93.70%; 
                     Adjusted-Interval-0:262/-436;
                     AiE0040m_C2"
                     /note="/Alternative_Match=93.70%; 
                     Adjusted-Interval-0:537/-161;
                     AiE0040m_C2"
                     /note="/Alternative_Match=92.13%; 
                     Adjusted-Interval-0:-13/-711;
                     AiE0040m_C2"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     AiE0050m_3xC 2"
                     /note="/Transferred_From=AiE0050m_3xC2"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Alternative_Match=100.00%;
                     Adjusted-Interval-0:113/-571; AiE0040m_C3"
                     /note="/X-Alternative_Match=100.00%;
                     Adjusted-Interval-0:388/-296; AiE0040m_C3"
                     /note="/X-Alternative_Match=100.00%;
                     Adjusted-Interval-0:663/-21; AiE0040m_C3"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     AiE0050m_3xC 2"
                     /note="/X-Transferred_From=AiE0050m_3xC2"
                     /note="/X-Transferred_Similarity=100.00%"
     promoter        987..1039
                     /label=minBG promoter
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     minBG
                     promoter (Beta Globin minimal promoter)"
                     /note="/Transferred_From=Beta Globin minimal promoter"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=yoav.ben-simon"
                     /note="/modified_by=yoav.ben-simon"
     regulatory      1066..1075
                     /label=Kozak sequence
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     Kozak sequence"
                     /note="/Transferred_From=Kozak sequence"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     Kozak sequence"
                     /note="/X-Transferred_From=Kozak sequence"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/modified_by=yoav.ben-simon"
     CDS             1075..1774
                     /label=dTomato
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=97.58%; Adjusted-Interval-0:0/0; 
                     dTomato"
                     /note="/Transferred_From=dTomato"
                     /note="/Transferred_Similarity=97.58%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     dTomato"
                     /note="/X-Transferred_From=dTomato"
                     /note="/X-Transferred_Similarity=100.00%"
     CDS             1801..2502
                     /label=dTomato
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=98.58%; Adjusted-Interval-0:0/0; 
                     dTomato"
                     /note="/Transferred_From=dTomato"
                     /note="/Transferred_Similarity=98.58%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     dTomato"
                     /note="/X-Transferred_From=dTomato"
                     /note="/X-Transferred_Similarity=100.00%"
     3'UTR           2537..2782
                     /label=WPRE3
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     WPRE3"
                     /note="/Transferred_From=WPRE3"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=yoav.ben-simon"
                     /note="/modified_by=yoav.ben-simon"
     polyA_signal    2796..2999
                     /label=BGH-pA
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     BGH-pA"
                     /note="/Transferred_From=BGH-pA"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=yoav.ben-simon"
     LTR             3022..3162
                     /label=ITR
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     ITR"
                     /note="/Transferred_From=ITR"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=yoav.ben-simon"
     rep_origin      3254..3560
                     /label=f1 Origin
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; f1 
                     Origin"
                     /note="/Transferred_From=f1 Origin"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     f1 Origin"
                     /note="/X-Transferred_From=f1 Origin"
                     /note="/X-Transferred_Similarity=100.00%"
     promoter        3974..4078
                     /gene="bla"
                     /label=AmpR promoter
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     AmpR promoter"
                     /note="/Transferred_From=AmpR promoter"
                     /note="/Transferred_Similarity=100.00%"
     misc_feature    4009..4016
                     /label=FRT3 spacer (FASEBJ 2011, 25, 1)
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     FRT3 spacer (FASEBJ 2011, 25, 1)"
                     /note="/Transferred_From=FRT3 spacer (FASEBJ 2011, 25, 1)"
                     /note="/Transferred_Similarity=100.00%"
     CDS             4079..4936
                     /label=AmpR
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     AmpR"
                     /note="/Transferred_From=AmpR"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=yoav.ben-simon"
                     /note="/modified_by=yoav.ben-simon"
     rep_origin      5087..5754
                     /label=pUC ori
                     /label=pUC\ori
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="/Primary_Match=100.00%; Adjusted-Interval-0:0/0; 
                     pUC\ori
                     (pUC ori)"
                     /note="/Transferred_From=pUC ori"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/vntifkey=33"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtaggca acttgttaaa aatctcagtc
      181 aaacttacat actatataga acagcatggt gaatttaggg cacatggata taaaatggaa
      241 gtttcttatt cagtagcagc aacttgtggg cacaggagtt ggcaaagata aaaatgtcca
      301 aagtcacaaa tacaatgtat agttagtcat aggtgctgtt atttgcctca aaaaatagac
      361 ttttattttg cctttctttt ctttaaccac actcaaaatt agagaacaga gacaaaaccc
      421 agcaggaaat aggcaacttg ttaaaaatct cagtcaaact tacatactat atagaacagc
      481 atggtgaatt tagggcacat ggatataaaa tggaagtttc ttattcagta gcagcaactt
      541 gtgggcacag gagttggcaa agataaaaat gtccaaagtc acaaatacaa tgtatagtta
      601 gtcataggtg ctgttatttg cctcaaaaaa tagactttta ttttgccttt cttttcttta
      661 accacactca aaattagaga acagagacaa aacccagcag gaaataggca acttgttaaa
      721 aatctcagtc aaacttacat actatataga acagcatggt gaatttaggg cacatggata
      781 taaaatggaa gtttcttatt cagtagcagc aacttgtggg cacaggagtt ggcaaagata
      841 aaaatgtcca aagtcacaaa tacaatgtat agttagtcat aggtgctgtt atttgcctca
      901 aaaaatagac ttttattttg cctttctttt ctttaaccac actcaaaatt agagaacaga
      961 gacaaaaccc agcaggaaat gagctcgggc tgggcataaa agtcagggca gagccatcta
     1021 ttgcttacat ttgcttctgg gatccagatc ttcgaagcta gcggcgccac catggtgagc
     1081 aagggcgagg aggtcatcaa agagttcatg cgcttcaagg tgcgcatgga gggctccatg
     1141 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
     1201 accgccaagc tgaaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc
     1261 ccccagttca tgtacggctc caaggcgtac gtgaagcacc ccgccgacat ccccgattac
     1321 aagaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
     1381 ggtctggtga ccgtgaccca ggactcctcc ctgcaggacg gcacgctgat ctacaaggtg
     1441 aagatgcgcg gcaccaactt cccccccgac ggccccgtaa tgcagaagaa gaccatgggc
     1501 tgggaggcct ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg cgagatccac
     1561 caggccctga agctgaagga cggcggccac tacctggtgg agttcaagac catctacatg
     1621 gccaagaagc ccgtgcaact gcccggctac tactacgtgg acaccaagct ggacatcacc
     1681 tcccacaacg aggactacac catcgtggaa cagtacgagc gctccgaggg ccgccaccac
     1741 ctgttcctgg ggcatggcac cggcagcacc ggcagcggca gctccggcac cgcctcctcc
     1801 gaggacaaca acatggccgt catcaaagag ttcatgcgct tcaaggtgcg catggagggc
     1861 tccatgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     1921 acccagaccg ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc
     1981 ctgtcccccc agttcatgta cggctccaag gcgtacgtga agcaccccgc cgacatcccc
     2041 gattacaaga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     2101 gacggcggtc tggtgaccgt gacccaggac tcctccctgc aggacggcac gctgatctac
     2161 aaggtgaaga tgcgcggcac caacttcccc cccgacggcc ccgtaatgca gaagaagacc
     2221 atgggctggg aggcctccac cgagcgcctg tacccccgcg acggcgtgct gaagggcgag
     2281 atccaccagg ccctgaagct gaaggacggc ggccactacc tggtggagtt caagaccatc
     2341 tacatggcca agaagcccgt gcaactgccc ggctactact acgtggacac caagctggac
     2401 atcacctccc acaacgagga ctacaccatc gtggaacagt acgagcgctc cgagggccgc
     2461 caccacctgt tcctgtacgg catggacgag ctgtacaagt aagtcgacgg cgcgccgcgg
     2521 ccgcgaattc gatatcataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     2581 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     2641 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttagtt
     2701 cttgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     2761 ttgggcactg acaattccgt ggctcgagag atcttcgact gtgccttcta gttgccagcc
     2821 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     2881 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     2941 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     3001 acgtgcggac cgagcggccg caggaacccc tagtgatgga gttggccact ccctctctgc
     3061 gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc
     3121 gggcggcctc agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt
     3181 ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc
     3241 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     3301 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     3361 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     3421 tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc
     3481 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     3541 cttgttccaa actggaacaa cactcaaccc tatctcgggc tattcttttg atttataagg
     3601 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     3661 gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc
     3721 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
     3781 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     3841 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
     3901 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     3961 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     4021 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     4081 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     4141 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     4201 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     4261 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     4321 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     4381 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     4441 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     4501 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     4561 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     4621 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     4681 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     4741 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     4801 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     4861 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     4921 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     4981 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     5041 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     5101 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     5161 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     5221 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     5281 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     5341 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     5401 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     5461 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     5521 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     5581 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     5641 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     5701 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgt
//
