LOCUS       CCM2(FL)_pET49b         7190 bp    DNA     circular  10-JUL-2024
FEATURES             Location/Qualifiers
     misc_feature    6961..6978
                     /label="thrombin site"
                     /color="#5A00FF"
     misc_feature    5568..5585
                     /label="HisTag"
                     /color="#5A00FF"
     promoter        4751..4769
                     /label="T7 promoter"
                     /color="#07D16D,#05E6CA"
     RBS             4809..4831
                     /label="RBS"
                     /color="#7FE3FF"
     misc_feature    5595..5618
                     /label="HRV-3C site"
                     /color="#5A00FF"
     protein_bind    4770..4794
                     /label="lac operator"
                     /color="#3E746E"
     misc_feature    6979..7023
                     /label="S-Tag"
                     /color="#5A00FF"
     terminator      7118..7165
                     /label="T7 terminator"
                     /color="#F10521,#fa1530"
     promoter        4361..4438
                     /label="lacI promoter"
                     /color="#07D16D,#05E6CA"
     misc_feature    2271..2413
                     /label="bom"
                     /color="#5A00FF"
     CDS             2515..2706
                     /label="rop"
                     /color="#FF33A2,#E6007D"
     rep_origin      12..467
                     /label="f1 ori"
                     /color="#02A3D0,#21bbfd"
     rep_origin      1497..2085
                     /label="ori"
                     /color="#02A3D0,#21bbfd"
     misc_feature    4839..5492
                     /label="GST"
                     /color="#5A00FF"
     CDS             560..1375
                     /label="KanR"
                     /color="#FF33A2,#E6007D"
     CDS             3278..4360
                     /label="lacI"
                     /color="#FF33A2,#E6007D"
     misc_feature    5622..6953
                     /label="CCM2(FL)"
                     /color="#5A00FF"
     CDS             4839..6956
                     /label="Expression construct"
                     /color="#FF33A2,#E6007D"
ORIGIN      
        1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
       61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
      301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
      361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
      421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt
      481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      541 tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat
      601 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa
      661 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc
      721 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga
      781 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc
      841 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac
      901 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcactg ttaaaaggac
      961 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat
     1021 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttgccg gggatcgcag
     1081 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca
     1141 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac
     1201 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg
     1261 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca
     1321 tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac
     1381 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa
     1441 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1501 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1561 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1621 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     1681 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1741 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1801 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1861 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1921 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1981 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     2041 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     2101 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     2161 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     2221 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
     2281 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta
     2341 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
     2401 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     2461 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     2521 gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc
     2581 gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag
     2641 aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt
     2701 ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa
     2761 acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg
     2821 ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg
     2881 tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc
     2941 tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta
     3001 cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca
     3061 gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc
     3121 ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgctagtca tgccccgcgc
     3181 ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc
     3241 ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     3301 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     3361 tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct
     3421 tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc
     3481 gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt
     3541 cgtatcccac taccgagatg tccgcaccaa cgcgcagccc ggactcggta atggcgcgca
     3601 ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat
     3661 tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg
     3721 ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg
     3781 ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca
     3841 gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg
     3901 tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa
     3961 tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa
     4021 gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca
     4081 cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt
     4141 gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt
     4201 gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg
     4261 ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac
     4321 cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac
     4381 tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg
     4441 ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg
     4501 aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt
     4561 cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag
     4621 tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct
     4681 gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat
     4741 cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta
     4801 gaaataattt tgtttaactt taagaaggag atatacatat gtcccctata ctaggttatt
     4861 ggaaaattaa gggccttgtg caacccactc gacttctttt ggaatatctt gaagaaaaat
     4921 atgaagagca tttgtatgag cgcgatgaag gtgataaatg gcgaaacaaa aagtttgaat
     4981 tgggtttgga gtttcccaat cttccttatt atattgatgg tgatgttaaa ttaacacagt
     5041 ctatggccat catacgttat atagctgaca agcacaacat gttgggtggt tgtccaaaag
     5101 agcgtgcaga gatttcaatg cttgaaggag cggttttgga tattagatac ggtgtttcga
     5161 gaattgcata tagtaaagac tttgaaactc tcaaagttga ttttcttagc aagctacctg
     5221 aaatgctgaa aatgttcgaa gatcgtttat gtcataaaac atatttaaat ggtgatcatg
     5281 taacccatcc tgacttcatg ttgtatgacg ctcttgatgt tgttttatac atggacccaa
     5341 tgtgcctgga tgcgttccca aaattagttt gttttaaaaa acgtattgaa gctatcccac
     5401 aaattgataa gtacttgaaa tccagcaagt atatagcatg gcctttgcag ggctggcaag
     5461 ccacgtttgg tggtggcgac catcctccaa aatcggatgg ttcaactagt ggtggtggcg
     5521 gttctaataa caatcctcct actcctactc catctagtgg ttctggtcat caccatcacc
     5581 atcactccgc ggctCTTGAA GTCCTCTTTC AGGGACCCGG TATGGAAGAA GAGGGGAAAA
     5641 AAGGTAAAAA ACCGGGTATT GTTAGCCCGT TCAAACGTGT TTTTCTGAAA GGTGAAAAAA
     5701 GCCGTGATAA AAAAGCCCAC GAAAAAGTTA CCGAACGTCG TCCGCTGCAT ACCGTTGTTC
     5761 TGAGCCTGCC GGAACGTGTT GAACCGGATC GTCTGCTGAG CGATTATATT GAAAAAGAGG
     5821 TGAAATATCT GGGCCAGCTG ACCAGCATTC CGGGTTATCT GAATCCGAGC AGCCGTACCG
     5881 AAATTCTGCA TTTTATTGAT AATGCCAAAC GTGCACATCA GCTGCCTGGT CATCTGACCC
     5941 AAGAACATGA TGCAGTGCTG AGCCTGAGCG CATATAATGT TAAACTGGCA TGGCGTGATG
     6001 GCGAAGATAT TATTCTGCGT GTTCCGATTC ATGATATTGC AGCAGTTAGC TATGTTCGTG
     6061 ATGATGCAGC ACATCTGGTT GTGCTGAAAA CCGCACAGGA TCCGGGTATT AGCCCGAGCC
     6121 AGAGCCTGTG TGCAGAAAGC AGCCGTGGTC TGAGCGCAGG TAGCCTGAGT GAAAGCGCAG
     6181 TTGGTCCGGT TGAAGCATGT TGTCTGGTTA TTCTGGCAGC CGAAAGCAAA GTTGCAGCCG
     6241 AAGAACTGTG TTGTCTGCTG GGTCAAGTTT TTCAGGTTGT TTATACCGAA AGCACCATCG
     6301 ATTTTCTGGA TCGTGCAATT TTTGATGGTG CAAGCACCCC GACACATCAT CTGAGTCTGC
     6361 ATTCAGATGA TAGCAGTACC AAAGTGGATA TCAAAGAAAC CTATGAAGTT GAAGCCAGCA
     6421 CCTTTTGTTT TCCGGAATCA GTTGATGTTG GTGGTGCAAG TCCGCATAGC AAAACCATTA
     6481 GCGAAAGCGA ACTGAGCGCA AGCGCAACCG AGCTGCTGCA GGATTACATG CTGACCCTGC
     6541 GTACCAAACT GAGCAGCCAA GAAATTCAGC AGTTTGCAGC CCTGCTGCAT GAATATCGTA
     6601 ATGGTGCCAG CATCCATGAA TTTTGCATTA ATCTGCGTCA GCTGTATGGT GATAGCCGTA
     6661 AATTTCTGCT GCTGGGCCTG CGTCCGTTTA TCCCGGAAAA AGATAGCCAG CATTTTGAAA
     6721 ACTTTCTGGA AACCATTGGT GTGAAAGATG GTCGTGGTAT TATTACCGAT AGCTTTGGTC
     6781 GTCATCGTCG TGCACTGAGC ACCACCAGTA GCAGCACCAC AAATGGTAAT CGTGCAACCG
     6841 GTAGCAGTGA TGATCGTAGC GCACCGAGCG AAGGTGATGA ATGGGATCGT ATGATTAGCG
     6901 ATATTAGCAG CGATATTGAA GCACTGGGTT GTAGCATGGA TCAGGATAGC GCATAAcgct
     6961 ctggtgccac gcggtagtaa agaaaccgct gctgctaaat tcgaacgcca gcacatggac
     7021 agctctactt ctgctgctct cgaggcttaa ttaacctagg ctgctaaaca aagcccgaaa
     7081 ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc
     7141 taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat 
//