LOCUS       Exported                7869 bp ds-DNA     circular SYN 10-JUL-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 12, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     >pDRf1-GW sequence 8606 bps
FEATURES             Location/Qualifiers
     source          1..7869
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..774
                     /locus_tag="pma1 promoter"
                     /label=pma1 promoter
     misc_feature    683..709
                     /locus_tag="primer 7"
                     /label=primer 7
     misc_feature    893..979
                     /locus_tag="Cyterm 29 aminoacids Rabbit cytochrome p450
                     sequenc"
                     /label=Cyterm 29 aminoacids Rabbit cytochrome
                     p450 seq...
                     /label=Cyterm 29 aminoacids Rabbit cytochrome p450
                     sequenc
     misc_feature    980..1007
                     /locus_tag="linker"
                     /label=linker
     CDS             1031..1747
                     /codon_start=1
                     /locus_tag="mGold mVenus(L46F;T63S)"
                     /product="Venus YFP with monomerizing A206K mutation
                     (Nagai et al., 2002)"
                     /label=mGold mVenus(L46F;T63S)
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTSLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    4124..4923
                     /locus_tag="URA3"
                     /label=URA3
     misc_feature    5355..6215
                     /locus_tag="amp resistentie"
                     /label=amp resistentie
     misc_feature    7298..7327
                     /locus_tag="lac promotor"
                     /label=lac promotor
ORIGIN
        1 caagcttcct gaaacggaga aacataaaca ggcattgctg ggatcaccca tacatcactc
       61 tgttttgcct gaccttttcc ggtaatttga aaacaaaccc ggtctcgaag cggagatccg
      121 gcgataatta ccgcagaaat aaacccatac acgagacgta gaaccagccg cacatggccg
      181 gagaaactcc tgcgagaatt tcgtaaactc gcgcgcattg catctgtatt tcctaatgcg
      241 gcacttccag gcctcgatcg agaccgttta tccattgctt ttttgttgtc tttttccctc
      301 gttcacagaa agtctgaaga agctatagta gaactatgag ctttttttgt ttctgttttc
      361 cttttttttt tttttacctc tgtggaaatt gttactctca cactctttag ttcgtttgtt
      421 tgttttgttt attccaatta tgaccggtga cgaaacgtgg tcgatggtgg gtaccgctta
      481 tgctcccctc cattagtttc gattatataa aaaggccaaa tattgtatta ttttcaaatg
      541 tcctatcatt atcgtctaac atctaatttc tcttaaattt tttctctttc tttcctataa
      601 caccaatagt gaaaatcttt ttttcttcta tatctacaaa aacttttttt ttctatcaac
      661 ctcgttgata aattttttct ttaacaatcg ttaataatta attaattgga aaataaccat
      721 tttttctctc ttttatacac acattcaaaa gaaagaaaaa aaatataccc cagcctcgaa
      781 ctagtgaatt cgcggccatc acaagtttgt acaaaaaagc aggctgctag cgctaccgga
      841 ctcagatctg gtaccccttg cagcttccag ttgtcaggaa gagaaggctc ccatggatcc
      901 tgtggtggtg ctggggctct gtctctcctg tttgcttctc ctttcactct ggaaacagag
      961 ctatggggga gggaagcttc gaattctgca gtcgacggta ccgcgggccc gggatccacc
     1021 ggtcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     1081 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     1141 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     1201 ctggcccacc ctcgtgacca gcctgggcta cggcctgcag tgcttcgccc gctaccccga
     1261 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     1321 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     1381 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     1441 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca ccgccgacaa
     1501 gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcggcgt
     1561 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     1621 cgacaaccac tacctgagct accagtccaa gctgagcaaa gaccccaacg agaagcgcga
     1681 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     1741 gtacaagtaa gcggccgcac ccagctttct tgtacaaagt ggtgtagtta attcatgatg
     1801 gccgctgcag gtcgacctcg agggggggcc cggtacccaa ttcgccctat agtgagtcgt
     1861 attacgcgcg gatccagctt tggacttctt cgccagaggt ttggtcaagt ctccaatcaa
     1921 ggttgtcggc ttgtctacct tgccagaaat ttacgaaaag atggaaaagg gtcaaatcgt
     1981 tggtagatac gttgttgaca cttctaaata agcgaatttc ttatgattta tgatttttat
     2041 tattaaataa gttataaaaa aaataagtgt atacaaattt taaagtgact cttaggtttt
     2101 aaaacganaa ttcttattct tgagtaactc tttcctgtag gtcaggttgc tttctcaggt
     2161 atagcatgag gtcgctctta ttgaccacac ctctaccggc atgccaattc actggccgtc
     2221 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     2281 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     2341 cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg
     2401 tgcggtattt cacaccgcat aatcggatcg tacttgttac ccatcattga attttgaaca
     2461 tccgaacctg ggagttttcc ctgaaacaga tagtatattt gaacctgtat aataatatat
     2521 agtctagcgc tttacggaag acaatgtatg tatttcggtt cctggagaaa ctattgcatc
     2581 tattgcatag gtaatcttgc acgtcgcatc cccggttcat tttctgcgtt tccatcttgc
     2641 acttcaatag catatctttg ttaacgaagc atctgtgctt cattttgtag aacaaaaatg
     2701 caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa
     2761 atgcaacgcg aaagcgctat tttaccaacg aagaatctgt gcttcatttt tgtaaaacaa
     2821 aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa
     2881 cagaaatgca acgcgagagc gctattttac caacaaagaa tctatacttc ttttttgttc
     2941 tacaaaaatg catcccgaga gcgctatttt tctaacaaag catcttagat tacttttttt
     3001 ctcctttgtg cgctctataa tgcagtctct tgataacttt ttgcactgta ggtccgttaa
     3061 ggttagaaga aggctacttt ggtgtctatt ttctcttcca taaaaaaagc ctgactccac
     3121 ttcccgcgtt tactgattac tagcgaagct gcgggtgcat tttttcaaga taaaggcatc
     3181 cccgattata ttctataccg atgtggattg cgcatacttt gtgaacagaa agtgatagcg
     3241 ttgatgattc ttcattggtc agaaaattat gaacggtttc ttctattttg tctctatata
     3301 ctacgtatag gaaatgttta cattttcgta ttgttttcga ttcactctat gaatagttct
     3361 tactacaatt tttttgtcta aagagtaata ctagagataa acataaaaaa tgtagaggtc
     3421 gagtttagat gcaagttcaa ggagcgaaag gtggatgggt aggttatata gggatatagc
     3481 acagagatat atagcaaaga gatacttttg agcaatgttt gtggaagcgg tattcgcaat
     3541 attttagtag ctcgttacag tccggtgcgt ttttggtttt ttgaaagtgc gtcttcagag
     3601 cgcttttggt tttcaaaagc gctctgaagt tcctatactt tctagctaga gaataggaac
     3661 ttcggaatag gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat gcaacgcgag
     3721 ctgcgcacat acagctcact gttcacgtcg cacctatatc tgcgtgttgc ctgtatatat
     3781 atatacatga gaagaacggc atagtgcgtg tttatgctta aatgcgtact tatatgcgtc
     3841 tatttatgta ggatgaaagg tagtctagta cctcctgtga tattatccca ttccatgcgg
     3901 ggtatcgtat gcttccttca gcactaccct ttagctgttc tatatgctgc cactcctcaa
     3961 ttggattagt ctcatccttc aatgctatca tttcctttga tattggatcg atccgatgat
     4021 aagctgtcaa acatgagaat tgggtaataa ctgatataat taaattgaag ctctaatttg
     4081 tgagtttagt atacatgcat ttacttataa tacagttttt tagttttgct ggccgcatct
     4141 tctcaaatat gcttcccagc ctgcttttct gtaacgttca ccctctacct tagcatccct
     4201 tccctttgca aatagtcctc ttccaacaat aataatgtca gatcctgtag agaccacatc
     4261 atccacggtt ctatactgtt gacccaatgc gtctcccttg tcatctaaac ccacaccggg
     4321 tgtcataatc aaccaatcgt aaccttcatc tcttccaccc atgtctcttt gagcaataaa
     4381 gccgataaca aaatctttgt cgctcttcgc aatgtcaaca gtacccttag tatattctcc
     4441 agtagatagg gagcccttgc atgacaattc tgctaacatc aaaaggcctc taggttcctt
     4501 tgttacttct tctgccgcct gcttcaaacc gctaacaata cctgggccca ccacaccgtg
     4561 tgcattcgta atgtctgccc attctgctat tctgtataca cccgcagagt actgcaattt
     4621 gactgtatta ccaatgtcag caaattttct gtcttcgaag agtaaaaaat tgtacttggc
     4681 ggataatgcc tttagcggct taactgtgcc ctccatggaa aaatcagtca agatatccac
     4741 atgtgttttt agtaaacaaa ttttgggacc taatgcttca actaactcca gtaattcctt
     4801 ggtggtacga acatccaatg aagcacacaa gtttgtttgc ttttcgtgca tgatattaaa
     4861 tagcttggca gcaacaggac taggatgagt agcagcacgt tccttatatg tagctttcga
     4921 catgatttat cttcgtttcc tgcatgtttt tgttctgtgc agttgggtta agaatactgg
     4981 gcaatttcat gtttcttcaa cactacatat gcgtatatat accaatctaa gtctgtgctc
     5041 cttccttcgt tcttccttct gttcggagat taccgaatca aaaaaatttc aaggaaaccg
     5101 aaatcaaaaa aaagaataaa aaaaaaatga tgaattgaaa agctaattct tgaagacgaa
     5161 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     5221 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     5281 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     5341 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     5401 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     5461 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     5521 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     5581 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     5641 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     5701 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     5761 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     5821 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     5881 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     5941 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     6001 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     6061 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     6121 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     6181 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     6241 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     6301 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     6361 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     6421 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     6481 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     6541 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     6601 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     6661 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     6721 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     6781 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     6841 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     6901 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     6961 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     7021 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     7081 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     7141 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     7201 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     7261 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     7321 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     7381 attacgccaa gcttaccgca tcaggaaatt gtaagcgtta atattttgtt aaaattcgcg
     7441 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
     7501 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     7561 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
     7621 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
     7681 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
     7741 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
     7801 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
     7861 tccattcgc
//
