LOCUS       Exported                7673 bp ds-DNA     linear   UNA 14-MAY-2024
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 12, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     >pDRf1-GW sequence 8606 bps
FEATURES             Location/Qualifiers
     source          1..7673
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     misc_feature    1..774
                     /locus_tag="pma1 promoter"
                     /label=pma1 promoter
     misc_feature    844..1548
                     /locus_tag="ymBaojin"
                     /label=ymBaojin
     misc_feature    3928..4727
                     /locus_tag="URA3"
                     /label=URA3
     misc_feature    5159..6019
                     /locus_tag="amp resistentie"
                     /label=amp resistentie
     misc_feature    7102..7131
                     /locus_tag="lac promotor"
                     /label=lac promotor
ORIGIN
        1 caagcttcct gaaacggaga aacataaaca ggcattgctg ggatcaccca tacatcactc
       61 tgttttgcct gaccttttcc ggtaatttga aaacaaaccc ggtctcgaag cggagatccg
      121 gcgataatta ccgcagaaat aaacccatac acgagacgta gaaccagccg cacatggccg
      181 gagaaactcc tgcgagaatt tcgtaaactc gcgcgcattg catctgtatt tcctaatgcg
      241 gcacttccag gcctcgatcg agaccgttta tccattgctt ttttgttgtc tttttccctc
      301 gttcacagaa agtctgaaga agctatagta gaactatgag ctttttttgt ttctgttttc
      361 cttttttttt tttttacctc tgtggaaatt gttactctca cactctttag ttcgtttgtt
      421 tgttttgttt attccaatta tgaccggtga cgaaacgtgg tcgatggtgg gtaccgctta
      481 tgctcccctc cattagtttc gattatataa aaaggccaaa tattgtatta ttttcaaatg
      541 tcctatcatt atcgtctaac atctaatttc tcttaaattt tttctctttc tttcctataa
      601 caccaatagt gaaaatcttt ttttcttcta tatctacaaa aacttttttt ttctatcaac
      661 ctcgttgata aattttttct ttaacaatcg ttaataatta attaattgga aaataaccat
      721 tttttctctc ttttatacac acattcaaaa gaaagaaaaa aaatataccc cagcctcgaa
      781 ctagtgaatt cgcggccatc acaagtttgt acaaaaaagc aggctgctag cccatggtct
      841 agaatggttt ctaagggtga agaagaaaac atggcttcta ctccattcaa gttccaattg
      901 aagggtacta ttaacggtaa gtctttcact gttgaaggtg aaggtgaagg taactctcat
      961 gaaggttctc acaagggtaa gtacgtttgt acttctggta agttgccaat gtcttgggct
     1021 gctttgggta ctactttcgg ttacggtatg aagtactaca ctaagtaccc atctggttta
     1081 aagaactggt tcagagaagt tatgccaggt ggtttcactt acgatagaca tattcaatac
     1141 aagggtgatg gttctattca tgctaagcat caacatttca tgaagaacgg tacttaccat
     1201 aacattgttg aattcactgg tcaagatttc aaggaaaatt ctccagtttt gaccggtgat
     1261 atgaacgttt ctttgccaaa tgaagttcca caaattccaa gagatgatgg tgttgaatgt
     1321 ccagttactt tattgtaccc attgttgtct gataagtcta agtatgttga agctcatcaa
     1381 tacactattt gtaagccatt gcataaccaa ccagctccag atgtcccata ccattggatt
     1441 agaaagcaat acactcaatc taaggatgat gctgaagaaa gagatcatat ttgtcaatct
     1501 gaaactttag aagctcattt gaagggtatg gatgaattgt acaagtaact cgaggcggcc
     1561 gcacccagct ttcttgtaca aagtggtgta gttaattcat gatggccgct gcaggtcgac
     1621 ctcgaggggg ggcccggtac ccaattcgcc ctatagtgag tcgtattacg cgcggatcca
     1681 gctttggact tcttcgccag aggtttggtc aagtctccaa tcaaggttgt cggcttgtct
     1741 accttgccag aaatttacga aaagatggaa aagggtcaaa tcgttggtag atacgttgtt
     1801 gacacttcta aataagcgaa tttcttatga tttatgattt ttattattaa ataagttata
     1861 aaaaaaataa gtgtatacaa attttaaagt gactcttagg ttttaaaacg anaattctta
     1921 ttcttgagta actctttcct gtaggtcagg ttgctttctc aggtatagca tgaggtcgct
     1981 cttattgacc acacctctac cggcatgcca attcactggc cgtcgtttta caacgtcgtg
     2041 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     2101 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     2161 atggcgaatg gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     2221 gcataatcgg atcgtacttg ttacccatca ttgaattttg aacatccgaa cctgggagtt
     2281 ttccctgaaa cagatagtat atttgaacct gtataataat atatagtcta gcgctttacg
     2341 gaagacaatg tatgtatttc ggttcctgga gaaactattg catctattgc ataggtaatc
     2401 ttgcacgtcg catccccggt tcattttctg cgtttccatc ttgcacttca atagcatatc
     2461 tttgttaacg aagcatctgt gcttcatttt gtagaacaaa aatgcaacgc gagagcgcta
     2521 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgaaagcg
     2581 ctattttacc aacgaagaat ctgtgcttca tttttgtaaa acaaaaatgc aacgcgagag
     2641 cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga
     2701 gagcgctatt ttaccaacaa agaatctata cttctttttt gttctacaaa aatgcatccc
     2761 gagagcgcta tttttctaac aaagcatctt agattacttt ttttctcctt tgtgcgctct
     2821 ataatgcagt ctcttgataa ctttttgcac tgtaggtccg ttaaggttag aagaaggcta
     2881 ctttggtgtc tattttctct tccataaaaa aagcctgact ccacttcccg cgtttactga
     2941 ttactagcga agctgcgggt gcattttttc aagataaagg catccccgat tatattctat
     3001 accgatgtgg attgcgcata ctttgtgaac agaaagtgat agcgttgatg attcttcatt
     3061 ggtcagaaaa ttatgaacgg tttcttctat tttgtctcta tatactacgt ataggaaatg
     3121 tttacatttt cgtattgttt tcgattcact ctatgaatag ttcttactac aatttttttg
     3181 tctaaagagt aatactagag ataaacataa aaaatgtaga ggtcgagttt agatgcaagt
     3241 tcaaggagcg aaaggtggat gggtaggtta tatagggata tagcacagag atatatagca
     3301 aagagatact tttgagcaat gtttgtggaa gcggtattcg caatatttta gtagctcgtt
     3361 acagtccggt gcgtttttgg ttttttgaaa gtgcgtcttc agagcgcttt tggttttcaa
     3421 aagcgctctg aagttcctat actttctagc tagagaatag gaacttcgga ataggaactt
     3481 caaagcgttt ccgaaaacga gcgcttccga aaatgcaacg cgagctgcgc acatacagct
     3541 cactgttcac gtcgcaccta tatctgcgtg ttgcctgtat atatatatac atgagaagaa
     3601 cggcatagtg cgtgtttatg cttaaatgcg tacttatatg cgtctattta tgtaggatga
     3661 aaggtagtct agtacctcct gtgatattat cccattccat gcggggtatc gtatgcttcc
     3721 ttcagcacta ccctttagct gttctatatg ctgccactcc tcaattggat tagtctcatc
     3781 cttcaatgct atcatttcct ttgatattgg atcgatccga tgataagctg tcaaacatga
     3841 gaattgggta ataactgata taattaaatt gaagctctaa tttgtgagtt tagtatacat
     3901 gcatttactt ataatacagt tttttagttt tgctggccgc atcttctcaa atatgcttcc
     3961 cagcctgctt ttctgtaacg ttcaccctct accttagcat cccttccctt tgcaaatagt
     4021 cctcttccaa caataataat gtcagatcct gtagagacca catcatccac ggttctatac
     4081 tgttgaccca atgcgtctcc cttgtcatct aaacccacac cgggtgtcat aatcaaccaa
     4141 tcgtaacctt catctcttcc acccatgtct ctttgagcaa taaagccgat aacaaaatct
     4201 ttgtcgctct tcgcaatgtc aacagtaccc ttagtatatt ctccagtaga tagggagccc
     4261 ttgcatgaca attctgctaa catcaaaagg cctctaggtt cctttgttac ttcttctgcc
     4321 gcctgcttca aaccgctaac aatacctggg cccaccacac cgtgtgcatt cgtaatgtct
     4381 gcccattctg ctattctgta tacacccgca gagtactgca atttgactgt attaccaatg
     4441 tcagcaaatt ttctgtcttc gaagagtaaa aaattgtact tggcggataa tgcctttagc
     4501 ggcttaactg tgccctccat ggaaaaatca gtcaagatat ccacatgtgt ttttagtaaa
     4561 caaattttgg gacctaatgc ttcaactaac tccagtaatt ccttggtggt acgaacatcc
     4621 aatgaagcac acaagtttgt ttgcttttcg tgcatgatat taaatagctt ggcagcaaca
     4681 ggactaggat gagtagcagc acgttcctta tatgtagctt tcgacatgat ttatcttcgt
     4741 ttcctgcatg tttttgttct gtgcagttgg gttaagaata ctgggcaatt tcatgtttct
     4801 tcaacactac atatgcgtat atataccaat ctaagtctgt gctccttcct tcgttcttcc
     4861 ttctgttcgg agattaccga atcaaaaaaa tttcaaggaa accgaaatca aaaaaaagaa
     4921 taaaaaaaaa atgatgaatt gaaaagctaa ttcttgaaga cgaaagggcc tcgtgatacg
     4981 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     5041 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     5101 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     5161 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     5221 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     5281 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     5341 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     5401 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     5461 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     5521 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     5581 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     5641 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     5701 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     5761 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     5821 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     5881 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     5941 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     6001 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     6061 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     6121 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     6181 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     6241 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     6301 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     6361 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     6421 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     6481 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     6541 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     6601 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     6661 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     6721 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     6781 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     6841 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     6901 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     6961 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     7021 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     7081 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     7141 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttac
     7201 cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     7261 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     7321 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     7381 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     7441 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     7501 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     7561 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
     7621 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtccatt cgc
//
