LOCUS       Exported                7901 bp ds-DNA     linear   UNA 15-MAY-2024
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 12, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     >pDRf1-GW sequence 8606 bps
FEATURES             Location/Qualifiers
     source          1..7901
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     misc_feature    1..774
                     /locus_tag="pma1 promoter"
                     /label=pma1 promoter
     misc_feature    844..1773
                     /locus_tag="yemiRFP670"
                     /label=yemiRFP670
     misc_feature    4156..4955
                     /locus_tag="URA3"
                     /label=URA3
     misc_feature    5387..6247
                     /locus_tag="amp resistentie"
                     /label=amp resistentie
     misc_feature    7330..7359
                     /locus_tag="lac promotor"
                     /label=lac promotor
ORIGIN
        1 caagcttcct gaaacggaga aacataaaca ggcattgctg ggatcaccca tacatcactc
       61 tgttttgcct gaccttttcc ggtaatttga aaacaaaccc ggtctcgaag cggagatccg
      121 gcgataatta ccgcagaaat aaacccatac acgagacgta gaaccagccg cacatggccg
      181 gagaaactcc tgcgagaatt tcgtaaactc gcgcgcattg catctgtatt tcctaatgcg
      241 gcacttccag gcctcgatcg agaccgttta tccattgctt ttttgttgtc tttttccctc
      301 gttcacagaa agtctgaaga agctatagta gaactatgag ctttttttgt ttctgttttc
      361 cttttttttt tttttacctc tgtggaaatt gttactctca cactctttag ttcgtttgtt
      421 tgttttgttt attccaatta tgaccggtga cgaaacgtgg tcgatggtgg gtaccgctta
      481 tgctcccctc cattagtttc gattatataa aaaggccaaa tattgtatta ttttcaaatg
      541 tcctatcatt atcgtctaac atctaatttc tcttaaattt tttctctttc tttcctataa
      601 caccaatagt gaaaatcttt ttttcttcta tatctacaaa aacttttttt ttctatcaac
      661 ctcgttgata aattttttct ttaacaatcg ttaataatta attaattgga aaataaccat
      721 tttttctctc ttttatacac acattcaaaa gaaagaaaaa aaatataccc cagcctcgaa
      781 ctagtgaatt cgcggccatc acaagtttgt acaaaaaagc aggctgctag cccatggtct
      841 agaatggctg aaggttctgt tgctagacaa ccagatttgt tgacttgtga acatgaagaa
      901 attcatttgg ctggttctat tcaaccacat ggtgctttgt tggtcgtttc tgaacatgac
      961 catagagtta ttcaagcctc tgctaatgcc gctgaattct taaacttggg ttctgtttta
     1021 ggtgttccat tagctgaaat tgatggtgat ttgttaatta agattttacc acatttagat
     1081 ccaaccgctg aaggtatgcc agttgctgtt agatgtagaa tcggtaatcc ttccactgaa
     1141 tactgtggtt tgatgcatag accaccagaa ggtggtttga ttattgaatt ggaaagagct
     1201 ggtccatcta ttgatttatc cggtactttg gctccagctt tggaaagaat tagaactgct
     1261 ggttctttaa gagctttgtg tgatgacact gttttgttat tccaacaatg tactggttat
     1321 gatagagtta tggtttacag attcgatgaa caaggtcatg gtttagtttt ctctgaatgt
     1381 catgtccctg gtttggaatc ttacttcggt aatagatacc catcctctac tgttccacaa
     1441 atggctagac aattatacgt tagacaaaga gttagagttt tggtcgatgt tacttaccaa
     1501 ccagttccat tggaaccaag attatcccca ttgactggta gagatttaga tatgtctggt
     1561 tgtttcttaa gatctatgtc tccttgtcat ttgcaattct tgaaggatat gggtgttaga
     1621 gctactttgg ccgtttcttt agttgttggt ggtaagttgt ggggtttggt tgtttgtcac
     1681 cattacttgc caagattcat tagattcgaa ttaagagcta tctgtaagag attggctgaa
     1741 agaattgcta ctagaattac cgctttagaa tcttaactcg aggcggccgc acccagcttt
     1801 cttgtacaaa gtggtgtagt taattcatga tggccgctgc aggtcgacct cgaggggggg
     1861 cccggtaccc aattcgccct atagtgagtc gtattacgcg cggatccagc tttggacttc
     1921 ttcgccagag gtttggtcaa gtctccaatc aaggttgtcg gcttgtctac cttgccagaa
     1981 atttacgaaa agatggaaaa gggtcaaatc gttggtagat acgttgttga cacttctaaa
     2041 taagcgaatt tcttatgatt tatgattttt attattaaat aagttataaa aaaaataagt
     2101 gtatacaaat tttaaagtga ctcttaggtt ttaaaacgan aattcttatt cttgagtaac
     2161 tctttcctgt aggtcaggtt gctttctcag gtatagcatg aggtcgctct tattgaccac
     2221 acctctaccg gcatgccaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     2281 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     2341 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc
     2401 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc ataatcggat
     2461 cgtacttgtt acccatcatt gaattttgaa catccgaacc tgggagtttt ccctgaaaca
     2521 gatagtatat ttgaacctgt ataataatat atagtctagc gctttacgga agacaatgta
     2581 tgtatttcgg ttcctggaga aactattgca tctattgcat aggtaatctt gcacgtcgca
     2641 tccccggttc attttctgcg tttccatctt gcacttcaat agcatatctt tgttaacgaa
     2701 gcatctgtgc ttcattttgt agaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca
     2761 aagaatctga gctgcatttt tacagaacag aaatgcaacg cgaaagcgct attttaccaa
     2821 cgaagaatct gtgcttcatt tttgtaaaac aaaaatgcaa cgcgagagcg ctaatttttc
     2881 aaacaaagaa tctgagctgc atttttacag aacagaaatg caacgcgaga gcgctatttt
     2941 accaacaaag aatctatact tcttttttgt tctacaaaaa tgcatcccga gagcgctatt
     3001 tttctaacaa agcatcttag attacttttt ttctcctttg tgcgctctat aatgcagtct
     3061 cttgataact ttttgcactg taggtccgtt aaggttagaa gaaggctact ttggtgtcta
     3121 ttttctcttc cataaaaaaa gcctgactcc acttcccgcg tttactgatt actagcgaag
     3181 ctgcgggtgc attttttcaa gataaaggca tccccgatta tattctatac cgatgtggat
     3241 tgcgcatact ttgtgaacag aaagtgatag cgttgatgat tcttcattgg tcagaaaatt
     3301 atgaacggtt tcttctattt tgtctctata tactacgtat aggaaatgtt tacattttcg
     3361 tattgttttc gattcactct atgaatagtt cttactacaa tttttttgtc taaagagtaa
     3421 tactagagat aaacataaaa aatgtagagg tcgagtttag atgcaagttc aaggagcgaa
     3481 aggtggatgg gtaggttata tagggatata gcacagagat atatagcaaa gagatacttt
     3541 tgagcaatgt ttgtggaagc ggtattcgca atattttagt agctcgttac agtccggtgc
     3601 gtttttggtt ttttgaaagt gcgtcttcag agcgcttttg gttttcaaaa gcgctctgaa
     3661 gttcctatac tttctagcta gagaatagga acttcggaat aggaacttca aagcgtttcc
     3721 gaaaacgagc gcttccgaaa atgcaacgcg agctgcgcac atacagctca ctgttcacgt
     3781 cgcacctata tctgcgtgtt gcctgtatat atatatacat gagaagaacg gcatagtgcg
     3841 tgtttatgct taaatgcgta cttatatgcg tctatttatg taggatgaaa ggtagtctag
     3901 tacctcctgt gatattatcc cattccatgc ggggtatcgt atgcttcctt cagcactacc
     3961 ctttagctgt tctatatgct gccactcctc aattggatta gtctcatcct tcaatgctat
     4021 catttccttt gatattggat cgatccgatg ataagctgtc aaacatgaga attgggtaat
     4081 aactgatata attaaattga agctctaatt tgtgagttta gtatacatgc atttacttat
     4141 aatacagttt tttagttttg ctggccgcat cttctcaaat atgcttccca gcctgctttt
     4201 ctgtaacgtt caccctctac cttagcatcc cttccctttg caaatagtcc tcttccaaca
     4261 ataataatgt cagatcctgt agagaccaca tcatccacgg ttctatactg ttgacccaat
     4321 gcgtctccct tgtcatctaa acccacaccg ggtgtcataa tcaaccaatc gtaaccttca
     4381 tctcttccac ccatgtctct ttgagcaata aagccgataa caaaatcttt gtcgctcttc
     4441 gcaatgtcaa cagtaccctt agtatattct ccagtagata gggagccctt gcatgacaat
     4501 tctgctaaca tcaaaaggcc tctaggttcc tttgttactt cttctgccgc ctgcttcaaa
     4561 ccgctaacaa tacctgggcc caccacaccg tgtgcattcg taatgtctgc ccattctgct
     4621 attctgtata cacccgcaga gtactgcaat ttgactgtat taccaatgtc agcaaatttt
     4681 ctgtcttcga agagtaaaaa attgtacttg gcggataatg cctttagcgg cttaactgtg
     4741 ccctccatgg aaaaatcagt caagatatcc acatgtgttt ttagtaaaca aattttggga
     4801 cctaatgctt caactaactc cagtaattcc ttggtggtac gaacatccaa tgaagcacac
     4861 aagtttgttt gcttttcgtg catgatatta aatagcttgg cagcaacagg actaggatga
     4921 gtagcagcac gttccttata tgtagctttc gacatgattt atcttcgttt cctgcatgtt
     4981 tttgttctgt gcagttgggt taagaatact gggcaatttc atgtttcttc aacactacat
     5041 atgcgtatat ataccaatct aagtctgtgc tccttccttc gttcttcctt ctgttcggag
     5101 attaccgaat caaaaaaatt tcaaggaaac cgaaatcaaa aaaaagaata aaaaaaaaat
     5161 gatgaattga aaagctaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata
     5221 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     5281 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     5341 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     5401 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     5461 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     5521 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     5581 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     5641 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     5701 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     5761 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     5821 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     5881 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     5941 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     6001 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     6061 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     6121 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     6181 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     6241 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     6301 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     6361 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     6421 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     6481 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     6541 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     6601 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     6661 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     6721 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     6781 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     6841 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     6901 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     6961 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     7021 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     7081 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     7141 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     7201 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     7261 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     7321 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     7381 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcttaccg catcaggaaa
     7441 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     7501 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     7561 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     7621 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     7681 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     7741 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     7801 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     7861 ccgccgcgct taatgcgccg ctacagggcg cgtccattcg c
//
