LOCUS       pSDC803-CONSTRUC        7066 bp    DNA     circular UNA 14-MAY-2024
DEFINITION  .
ACCESSION   urn.local.sarahdc.s-fcxzol6
VERSION     urn.local.sarahdc.s-fcxzol6
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     gene            37..1470
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Ac_3_flank"
     misc_feature    1558..1864
                     /note="Geneious type: origin of replication"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="f1 origin"
     regulatory      2313..2341
                     /note="Geneious type: promoter"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="amp prom"
     misc_feature    2383..3243
                     /note="Geneious type: marker"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="amp marker"
     misc_feature    3398..4017
                     /note="Geneious type: origin of replication"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="pBR322 origin"
     misc_feature    complement(3642..3660)
                     /note="Geneious type:  "
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="VHL overhnags"
     gene            4239..5385
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Ac_5_flank"
     gene            complement(5396..5429)
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="loxP"
     gene            5396..5429
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="loxP; loxH"
     regulatory      complement(5454..5724)
                     /note="Geneious type: terminator"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="TK_PA term"
     misc_feature    complement(5745..6422)
                     /note="Geneious type: reporter"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="DsRed2 reporter"
     regulatory      6585..6685
                     /note="Geneious type: promoter"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="PH prom"
     source          <6698..6766
                     /organism="pHYE026_EloB in pFI003_pAC8RedNK_Flag"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
     misc_feature    6698..6724
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Flag tag"
     misc_feature    6737..6757
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="TEV"
     misc_feature    6767..7066
                     /note="Geneious type:  "
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="INSERT IKZF mut stop"
     misc_feature    complement(7042..7066)
                     /note="Geneious type:  "
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="INSERT"
ORIGIN      
        1 ggtacccccg gacggccgct taattaattg atccgggtta ttagtacatt tattaagcgc
       61 tagattctgt gcgttgttga tttacagaca attgttgtac gtattttaat aattcattaa
      121 atttataatc tttagggtgg tatgttagag cgaaaatcaa atgattttca gcgtctttat
      181 atctgaattt aaatattaaa tcctcaatag atttgtaaaa taggtttcga ttagtttcaa
      241 acaagggttg tttttccgaa ccgatggctg gactatctaa tggattttcg ctcaacgcca
      301 caaaacttgc caaatcttgt agcagcaatc tagctttgtc gatattcgtt tgtgttttgt
      361 tttgtaataa aggttcgacg tcgttcaaaa tattatgcgc ttttgtattt ctttcatcac
      421 tgtcgttagt gtacaattga ctcgacgtaa acacgttaaa taaagcttgg acatatttaa
      481 catcgggcgt gttagcttta ttaggccgat tatcgtcgtc gtcccaaccc tcgtcgttag
      541 aagttgcttc cgaagacgat tttgccatag ccacacgacg cctattaatt gtgtcggcta
      601 acacgtccgc gatcaaattt gtagttgagc tttttggaat tatttctgat tgcgggcgtt
      661 tttgggcggg tttcaatcta actgtgcccg attttaattc agacaacacg ttagaaagcg
      721 atggtgcagg cggtggtaac atttcagacg gcaaatctac taatggcggc ggtggtggag
      781 ctgatgataa atctaccatc ggtggaggcg caggcggggc tggcggcgga ggcggaggcg
      841 gaggtggtgg cggtgatgca gacggcggtt taggctcaaa tgtctcttta ggcaacacag
      901 tcggcacctc aactattgta ctggtttcgg gcgccgtttt tggtttgacc ggtctgagac
      961 gagtgcgatt tttttcgttt ctaatagctt ccaacaattg ttgtctgtcg tctaaaggtg
     1021 cagcgggttg aggttccgtc ggcattggtg gagcgggcgg caattcagac atcgatggtg
     1081 gtggtggtgg tggaggcgct ggaatgttag gcacgggaga aggtggtggc ggcggtgccg
     1141 ccggtataat ttgttctggt ttagtttgtt cgcgcacgat tgtgggcacc ggcgcaggcg
     1201 ccgctggctg cacaacggaa ggtcgtctgc ttcgaggcag cgcttggggt ggtggcaatt
     1261 caatattata attggaatac aaatcgtaaa aatctgctat aagcattgta atttcgctat
     1321 cgtttaccgt gccgatattt aacaaccgct caatgtaagc aattgtattg taaagagatt
     1381 gtctcaagct cggatcgatc ccgcacgccg ataacaagcc ttttcatttt tactacagca
     1441 ttgtagtggc gagacacttc gctgtcgtcg cctgatgcgg tattttctcc ttacgcatct
     1501 gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca
     1561 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     1621 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     1681 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     1741 cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     1801 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     1861 acaacactca accctatctc gggctattct tttgatttat aagggatttt gccgatttcg
     1921 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     1981 ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     2041 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     2101 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
     2161 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt
     2221 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     2281 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     2341 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     2401 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     2461 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     2521 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     2581 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     2641 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     2701 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     2761 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     2821 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     2881 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     2941 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     3001 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     3061 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     3121 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     3181 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     3241 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     3301 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     3361 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     3421 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     3481 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     3541 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     3601 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     3661 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     3721 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     3781 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     3841 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     3901 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     3961 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     4021 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     4081 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     4141 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     4201 ccgcctctcc ccgcgcgttg gccgattcat taatgcagaa cggctccgcc cactattaat
     4261 gaaattaaaa attccaattt taaaaaacgc agcaagagaa acatttgtat gaaagaatgc
     4321 gtagaaggaa agaaaaatgt cgtcgacatg ctgaacaaca agattaatat gcctccgtgt
     4381 ataaaaaaaa tattgaacga tttgaaagaa aacaatgtac cgcgcggcgg tatgtacagg
     4441 aagaggttta tactaaactg ttacattgca aacgtggttt cgtgtgccaa gtgtgaaaac
     4501 cgatgtttaa tcaaggctct gacgcatttc tacaaccacg actccaagtg tgtgggtgaa
     4561 gtcatgcatc ttttaatcaa atcccaagat gtgtataaac caccaaactg ccaaaaaatg
     4621 aaaactgtcg acaagctctg tccgtttgct ggcaactgca agggtctcaa tcctatttgt
     4681 aattattgaa taataaaaca attataaatg ctaaatttgt tttttattaa cgatacaaac
     4741 caaacgcaac aagaacattt gtagtattat ctataattga aaacgcgtag ttataatcgc
     4801 tgaggtaata tttaaaatca ttttcaaatg attcacagtt aatttgcgac aatataattt
     4861 tattttcaca taaactagac gccttgtcgt cttcttcttc gtattccttc tctttttcat
     4921 ttttctcctc ataaaaatta acatagttat tatcgtatcc atatatgtat ctatcgtata
     4981 gagtaaattt tttgttgtca taaatatata tgtctttttt aatggggtgt atagtaccgc
     5041 tgcgcatagt ttttctgtaa tttacaacag tgctattttc tggtagttct tcggagtgtg
     5101 ttgctttaat tattaaattt atataatcaa tgaatttggg atcgtcggtt ttgtacaata
     5161 tgttgccggc atagtacgca gcttcttcta gttcaattac accatttttt agcagcaccg
     5221 gattaacata actttccaaa atgttgtacg aaccgttaaa caaaaacagt tcacctccct
     5281 tttctatact attgtctgcg agcagttgtt tgttgttaaa aataacagcc attgtaatga
     5341 gacgcacaaa ctaatatcac aaactggaaa tgtctatcaa tatatagttg ctgatataac
     5401 ttcgtatagc atacattata cgaagttatc tggttgaaac gtacccgtag tggctatggc
     5461 agggcttgcc gccccgacgt tggctgcgag ccctgggcct tcacccgaac ttgggggttg
     5521 gggtggggaa aaggaagaaa cgcgggcgta ttggtcccaa tggggtctcg gtggggtatc
     5581 gacagagtgc cagccctggg accgaacccc gcgtttatga acaaacgacc caacacccgt
     5641 gcgttttatt ctgtcttttt attgccgtca tagcgcgggt tccttccggt attgtctcct
     5701 tccgtgtttc agttagcctc ccccatctcc cgctattact cgagtcactg ggagccggag
     5761 tggcgggcct cggcgtgctc gtactgctcc accacggtgt agtcctcgtt gtggttggtg
     5821 atgtccagct tggagtccac gtagtggttg ccgggcagct gcacgggctt cttggccttg
     5881 tacacggtct tgaagtcgca ggtgtagtgg ccgccgtcct tcagcttcag ggcgtgggag
     5941 atctcgccct tcagcacgcc gtcctggggg tacagcttct cggtggaggg ctcccagccg
     6001 gcagtcttct tctgcattac ggggccgtcg gcggggaagt tcacgccctt gaacttcacc
     6061 ttgtagatga aggtgccgtc ctgcagggag gagtcctgct gcacctccac cacgccgccg
     6121 tcctcgaagt tcatggagcg ctcccaggtg aagccctcgg ggaaggacag cttcatgtag
     6181 tcggggatgt cggcggggtg cttcacgtag gccttggagc cgtactggaa ctggggggac
     6241 aggatgtccc aggcgaaggg cagggggccg cccttggtca cctgcagctt ggcggtctgg
     6301 gtgccctcgt agggcttgcc ctcgccctcg ccctcgatct cgaagtagtg gccgttcacg
     6361 gagccctcca tgcgcacctt gaactgcatg aactccttga tgacgtcctc ggtgttgtcc
     6421 atggtagagt gattgtaaat aaaatgtaat ttacagtata gtattttaat taatatacaa
     6481 atgatttgat aataattctt atttaactat aatatattgt gttgggttga attaaaggtc
     6541 cgtatactag tatcgattcg cgacctactc cggaatatta atagagttgc tgatatcatg
     6601 gagataatta aaatgataac catctcgcaa ataaataagt attttactgt tttcgtaaca
     6661 gttttgtaat aaaaaaacct ataaatacgg atctaccatg gactacaaag acgatgacga
     6721 caagtccgcg gtcgacgaga acctctactt ccagggaggc ggccgcatga cgggcgaacg
     6781 tcccttcgcg tgtaatcagt gtaacgcttc cttcacgcag aagggtaatt tgctccgaca
     6841 catcaagttg cattcgggtg agaagccctt caagtgccac ctctgtaatt atgcttgccg
     6901 ccgccgtgat gctctcaccg gtcacttgag aacacactcg taataaacag gagagagacc
     6961 ttttgcttgc aaccaatgca atgcgtcatt tactcaaaag ggaaacctct tgagacatat
     7021 aaaattacac tcaggagaga aaccatttaa atgtcatttg tgcaac
//
