LOCUS       Exported                6514 bp ds-DNA     circular SYN 12-JUL-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6514)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jul 15, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6514
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     20..39
                     /locus_tag="T7"
                     /label=T7
     misc_binding    39..61
                     /locus_tag="LacO"
                     /label=LacO
     misc_feature    94..100
                     /locus_tag="Ribsome binding"
                     /label=Ribsome binding
     misc_feature    101..106
                     /locus_tag="XbaI"
                     /label=XbaI
     misc_feature    1258..1263
                     /locus_tag="XhoI"
                     /label=XhoI
     misc_feature    1264..1281
                     /locus_tag="his tag"
                     /label=his tag
     rep_origin      1432..1887
                     /locus_tag="M13 origin"
                     /label=M13 origin
     rep_origin      1449..1755
                     /locus_tag="F1 ori"
                     /label=F1 ori
     CDS             2217..2876
                     /locus_tag="AmpR"
                     /label=AmpR
     rep_origin      2974..3656
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
ORIGIN
        1 gatctcgatc ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat
       61 tcccctctag taataatttt gtttaacttt aagaaggagg tctagatgag ctccaccgct
      121 ggacaggtta tcaactgcaa ggcagccgtc gcctggagcg caaaagctcc actctcgatt
      181 gagacgatcc aagtggctcc accgaaagcc cacgaagttc gagtgaaaat cctctacacc
      241 gcagtctgcc acacggacgc ctacaccctc gacggacacg atccggaagg tctcttccct
      301 gtcgtcttgg gacatgaagg gtctggaatt gtcgagagcg tcggagaagg agtcacagga
      361 tttgctccag gagatcacgt cgttccgctc tacgttcctc agtgcaaaga atgtgaatat
      421 tgcaagaatc caaagacgaa tctgtgtcaa aagatcagaa tctcgcaggg aaatggattt
      481 atgcccgatg gttcatcgag attcacatgc aacggaaagc agttgttcca ttttatggga
      541 tgctccacgt tcagtgaata cactgttgtg gccgacatct cactgtgcaa agtgaatcca
      601 gaagctccgc tcgagaaggt ttcgcttttg ggatgtggaa tttcaactgg atacggagcc
      661 gtgctgaaca cgtgcaaagt ggaagaaggg agtactgtag ccgtatgggg actcggtgca
      721 gtcggattgg ccgttatcat gggcgcaaag gctgccggag ccaagaaaat cgtcggaatc
      781 gacctgatcg agtcgaaatt cgagtcggcc aaattcttcg gagccaccga atgcatcaat
      841 ccgaagagtg ttgagcttcc tgaggggaag agcttccaag catggctcgt ggagcaattt
      901 gacggaggat ttgactacac ttttgagtgt attggaaatg tgcacacaat gcgccaagcc
      961 ctcgaagccg cccacaaagg atggggagtc tcgtgtatca tcggagtcgc cggagccggc
     1021 caagagatcg ccacccgtcc attccaactt gtcaccgggc gcacgtggaa gggaaccgca
     1081 ttcggcggat ggaaatccgt ggaaagtgtg ccgagactcg tcgacgacta catgaacaag
     1141 aagctgctca tcgatgaatt cattactcat cgctggaaca ttgacgatat caatacggcg
     1201 ttcgatgttc ttcacaaggg agagagtctg cggtcggtgc tggcgttcga gaaaatactc
     1261 gagcaccacc accaccacca ctgagatccg gctgctaaca aagcccgaaa ggaagctgag
     1321 ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc
     1381 ttgaggggtt ttttgctgaa aggaggaact atatccggat tggcgaatgg gacgcgccct
     1441 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     1501 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     1561 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     1621 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     1681 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     1741 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     1801 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     1861 ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa
     1921 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     1981 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     2041 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     2101 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     2161 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     2221 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     2281 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     2341 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     2401 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     2461 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     2521 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt
     2581 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     2641 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     2701 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     2761 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     2821 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     2881 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     2941 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     3001 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     3061 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     3121 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     3181 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     3241 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     3301 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     3361 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     3421 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     3481 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     3541 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     3601 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     3661 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     3721 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     3781 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc
     3841 tccttacgca tctgtgcggt atttcacacc gcaatggtgc actctcagta caatctgctc
     3901 tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct
     3961 gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     4021 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     4081 tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat
     4141 tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat
     4201 gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat
     4261 gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg
     4321 atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt
     4381 aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag
     4441 cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag
     4501 atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg
     4561 aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt
     4621 cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct
     4681 agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg
     4741 ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg
     4801 agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga
     4861 aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg
     4921 ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag
     4981 ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc
     5041 taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     5101 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag
     5161 ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc
     5221 ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt
     5281 gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac
     5341 cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc
     5401 catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat
     5461 ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat
     5521 ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact
     5581 taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc
     5641 cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac
     5701 atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc
     5761 atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc
     5821 cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag
     5881 ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact
     5941 ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt
     6001 gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac
     6061 gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc
     6121 gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg
     6181 ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct
     6241 ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca
     6301 ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg
     6361 ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat
     6421 cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga
     6481 tgccggccac gatgcgtccg gcgtagagga tcga
//
