LOCUS       Exported                6929 bp ds-DNA     circular SYN 21-JAN-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jul 15, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6929
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     20..39
                     /label=T7
     misc_binding    39..61
                     /label=LacO
     misc_feature    94..100
                     /label=Ribsome binding
     misc_feature    107..109
                     /label=Start codon
     misc_feature    1679..1696
                     /label=his tag
     rep_origin      1847..2302
                     /label=M13 origin
     rep_origin      1864..2170
                     /label=F1 ori
     CDS             2632..3291
                     /label=AmpR
     rep_origin      3389..4071
                     /label=ColE1 origin
ORIGIN
        1 gatctcgatc ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat
       61 tcccctctag taataatttt gtttaacttt aagaaggagg tctagaatgg catgcaaacg
      121 tcattcagtg tccgatccca tttcgaagga tctcgtcgag tgtcttcgtg aatcaatgca
      181 aagagatctc aaatttgaga ctccgtacgt tttcgtgata tttggagcat cgggagatct
      241 cgcaaaaaag aaaatatacc caacactgtg gtggttgttc cgtgacaacc ttttgccagt
      301 caacatcaaa tttattggat atgcccgttc cgatctgaca gttttcaagc ttcgtgaatc
      361 ttttgagaaa aactgtaaag ttcgagaaaa tgagaaatgt gcttttgatg acttcatcaa
      421 gaagtgctca tacgttcagg gccaatacga cacttctgaa ggattccaac gccttcaatc
      481 ttcaattgat gatttccaaa aagaaagcaa taatcaagcc gtcaatcgtc tctactacct
      541 cgctcttcct ccatcagttt tcaacgtggt ttctacggaa ttaaagaaga attgtatgga
      601 tcatggtgat tcatggacac gtgttatcat agaaaaacca tttggacatg atttgaaatc
      661 atcttgtgaa ctttcaactc atttggcgaa actttttaaa gaagatcaaa tctaccgtat
      721 tgatcattat ctcggaaaag aaatggttca gaatttgatg gttatgcgat ttggaaaccg
      781 aattctcgct ccatcttgga accgcgatca cattgcatca gttatgatct catttaaaga
      841 agattttgga actggtggcc gtgctggata ttttgataca gctggaatta tccgtgatgt
      901 tatgcagaat catttgatgc aaattctaac tttagtcgcc atggagaaac cagcaagttt
      961 gaatgctgaa gatattcgtg atgaaaaggt caaagtgttg aaagctgcta aagttgtaga
     1021 attaaaggat gtagtagttg gacaatacat tgccagtcct gaatttgatc acccagaagc
     1081 atcacaagga tacaaagatg acaaatcagt tccagccgat tcgactactc caacgtatgc
     1141 tcttgctgtt gttcacatca ataatgaacg ttgggagggt gttccattct tcttgcgttg
     1201 tggaaaagct ctcaacgaga agaaagcaga agttcgtata caattcaaag aagtcagtgg
     1261 agatatctat ccgtctggtg aattaaagcg atccgaatta gtgatgagag tgcaaccaaa
     1321 tgaggcagtt tacatgaaac tgatgaccaa gaaacctgga atgggattcg gtgttgaaga
     1381 aactgaactt gatttgactt ataataatag attcaaagaa gtccgtcttc ctgatgctta
     1441 tgaacgccta tttctcgaag tattcatggg atctcaaatt aatttcgttc gaactgatga
     1501 gcttgaatac gcttggcgta ttttgacgcc agttctcgaa gagcttaaaa agaagaaagt
     1561 tcaaccggtt caatataaat ttggaagtcg tggtccaact gaaggtgacg aattgatgaa
     1621 aaagtacggt ttcatcttca ctggaacata caaatgggtt gctccaaagc tactcgagca
     1681 ccaccaccac caccactgag atccggctgc taacaaagcc cgaaaggaag ctgagttggc
     1741 tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag
     1801 gggttttttg ctgaaaggag gaactatatc cggattggcg aatgggacgc gccctgtagc
     1861 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     1921 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     1981 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     2041 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     2101 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     2161 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     2221 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     2281 aaaatattaa cgtttacaat ttcaggtggc acttttcggg gaaatgtgcg cggaacccct
     2341 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     2401 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     2461 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     2521 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     2581 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     2641 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     2701 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     2761 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     2821 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     2881 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     2941 gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc
     3001 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     3061 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     3121 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     3181 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     3241 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     3301 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     3361 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     3421 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     3481 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     3541 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     3601 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     3661 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     3721 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     3781 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     3841 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     3901 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     3961 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     4021 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     4081 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     4141 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     4201 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt
     4261 acgcatctgt gcggtatttc acaccgcaat ggtgcactct cagtacaatc tgctctgatg
     4321 ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc
     4381 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     4441 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     4501 accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca
     4561 gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg
     4621 gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc
     4681 cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct
     4741 cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca
     4801 actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt
     4861 cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg
     4921 gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc
     4981 gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt
     5041 tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg
     5101 ggtcctcaac gacaggagca cgatcatgcg cacccgtggg gccgccatgc cggcgataat
     5161 ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc
     5221 gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg
     5281 gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa
     5341 gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac
     5401 tgggttgaag gctctcaagg gcatcggtcg agatcccggt gcctaatgag tgagctaact
     5461 tacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     5521 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gccagggtgg
     5581 tttttctttt caccagtgag acgggcaaca gctgattgcc cttcaccgcc tggccctgag
     5641 agagttgcag caagcggtcc acgctggttt gccccagcag gcgaaaatcc tgtttgatgg
     5701 tggttaacgg cgggatataa catgagctgt cttcggtatc gtcgtatccc actaccgaga
     5761 tatccgcacc aacgcgcagc ccggactcgg taatggcgcg cattgcgccc agcgccatct
     5821 gatcgttggc aaccagcatc gcagtgggaa cgatgccctc attcagcatt tgcatggttt
     5881 gttgaaaacc ggacatggca ctccagtcgc cttcccgttc cgctatcggc tgaatttgat
     5941 tgcgagtgag atatttatgc cagccagcca gacgcagacg cgccgagaca gaacttaatg
     6001 ggcccgctaa cagcgcgatt tgctggtgac ccaatgcgac cagatgctcc acgcccagtc
     6061 gcgtaccgtc ttcatgggag aaaataatac tgttgatggg tgtctggtca gagacatcaa
     6121 gaaataacgc cggaacatta gtgcaggcag cttccacagc aatggcatcc tggtcatcca
     6181 gcggatagtt aatgatcagc ccactgacgc gttgcgcgag aagattgtgc accgccgctt
     6241 tacaggcttc gacgccgctt cgttctacca tcgacaccac cacgctggca cccagttgat
     6301 cggcgcgaga tttaatcgcc gcgacaattt gcgacggcgc gtgcagggcc agactggagg
     6361 tggcaacgcc aatcagcaac gactgtttgc ccgccagttg ttgtgccacg cggttgggaa
     6421 tgtaattcag ctccgccatc gccgcttcca ctttttcccg cgttttcgca gaaacgtggc
     6481 tggcctggtt caccacgcgg gaaacggtct gataagagac accggcatac tctgcgacat
     6541 cgtataacgt tactggtttc acattcacca ccctgaattg actctcttcc gggcgctatc
     6601 atgccatacc gcgaaaggtt ttgcgccatt cgatggtgtc cgggatctcg acgctctccc
     6661 ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc
     6721 gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct
     6781 gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc
     6841 ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg
     6901 gccacgatgc gtccggcgta gaggatcga
//
