LOCUS       Exported                6457 bp ds-DNA     circular SYN 12-JUL-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6457)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jul 15, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6457
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     20..39
                     /locus_tag="T7"
                     /label=T7
     misc_binding    39..61
                     /locus_tag="LacO"
                     /label=LacO
     misc_feature    94..100
                     /locus_tag="Ribsome binding"
                     /label=Ribsome binding
     misc_feature    101..106
                     /locus_tag="XbaI"
                     /label=XbaI
     misc_feature    1201..1206
                     /locus_tag="XhoI"
                     /label=XhoI
     misc_feature    1207..1224
                     /locus_tag="his tag"
                     /label=his tag
     rep_origin      1375..1830
                     /locus_tag="M13 origin"
                     /label=M13 origin
     rep_origin      1392..1698
                     /locus_tag="F1 ori"
                     /label=F1 ori
     CDS             2160..2819
                     /locus_tag="AmpR"
                     /label=AmpR
     rep_origin      2917..3599
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
ORIGIN
        1 gatctcgatc ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat
       61 tcccctctag taataatttt gtttaacttt aagaaggagg tctagatggc ttcttactcg
      121 caattcttga ccaaggccca ggaggacgaa ctccgctcca tcgccaacgc catcgtcacc
      181 ccaggaaagg gaattctcgc cgccgatgag tccaccggat ccatggacaa gcgtcttaac
      241 agcatcggac tcgagaacac cgaggagaac cgccgcaagt atcgtcaact tctgtttacc
      301 gccggagccg acctcaacaa gtacatctcg ggagtcatca tgttccacga gacattctac
      361 caaaagaccg acgacggaaa gccattcact gctcttctcc aggagcaagg aatcattcca
      421 ggaatcaagg ttgacaaggg tgtcgtccca atggccggaa ccatcggaga gggaaccact
      481 cagggattgg atgacttgaa tgctcgttgc gctcaataca agaaagacgg agcacagttc
      541 gccaagtgga gatgcgtgca caagatctca tctacgactc catcagtgac tgctcttaag
      601 gaaattgcct ccaacttggc tcgctatgct tcaatttgcc aacaaaatgg acttgtacca
      661 attgttgagc cagagatcct cccagacggt gaacactgct tggctcgcgg acagaagatc
      721 accgagaccg tgctctcata tgtgtaccac gctctcaacg agcatcacgt cttcctcgag
      781 ggaaccctcc tcaagccaaa catggtcacc tccggacaat cattcaccgg cgagaagcca
      841 tcgaatgccg atatcggact cgccaccgtc accgccctcc aacgcggagt cccatccgcc
      901 gttccaggag tcgtcttcct ctccggtgga caatccgaag aggatgccac attgaacttg
      961 aacgccatca atcaagtgag cggaaagaag ccatgggccc tcacattctc ctatggacgt
     1021 gctcttcaag cctcctgtct cgccaagtgg gccggaaagg acgagaacat cgcagccgcc
     1081 caagaagtcc tgctccatcg tgctcaggtc aactcccttg cctctgtcgg aaaatacacc
     1141 ggggatgctt cagctgacgc agccgcttcc caatccctct tcgtcgccaa tcattcttat
     1201 ctcgagcacc accaccacca ccactgagat ccggctgcta acaaagcccg aaaggaagct
     1261 gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg
     1321 gtcttgaggg gttttttgct gaaaggagga actatatccg gattggcgaa tgggacgcgc
     1381 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     1441 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     1501 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     1561 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     1621 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     1681 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     1741 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     1801 attttaacaa aatattaacg tttacaattt caggtggcac ttttcgggga aatgtgcgcg
     1861 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     1921 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     1981 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     2041 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     2101 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     2161 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     2221 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     2281 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     2341 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     2401 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     2461 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa
     2521 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     2581 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     2641 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     2701 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     2761 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     2821 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     2881 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     2941 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     3001 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     3061 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     3121 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     3181 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     3241 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     3301 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     3361 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     3421 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     3481 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     3541 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     3601 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     3661 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     3721 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt
     3781 ttctccttac gcatctgtgc ggtatttcac accgcaatgg tgcactctca gtacaatctg
     3841 ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg
     3901 gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     3961 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
     4021 ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc
     4081 gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt
     4141 aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact
     4201 gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag
     4261 aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag
     4321 ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc
     4381 cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg
     4441 cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca
     4501 cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg
     4561 cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag
     4621 cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg
     4681 gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga
     4741 gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag
     4801 cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc
     4861 atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag
     4921 gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg
     4981 agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     5041 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     5101 cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg
     5161 gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg
     5221 tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac
     5281 taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag
     5341 cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg
     5401 catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg
     5461 aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga
     5521 acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac
     5581 gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga
     5641 gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg
     5701 gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac
     5761 cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc
     5821 cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag
     5881 actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg
     5941 gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga
     6001 aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc
     6061 tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg
     6121 gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac
     6181 gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga
     6241 gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca
     6301 cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc
     6361 gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg
     6421 tgatgccggc cacgatgcgt ccggcgtaga ggatcga
//
