LOCUS       AiP15041                5451 bp    DNA     circular UNA 01-MAY-2024
DEFINITION  pAAV-AiE1417m-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE-BGHpA.
ACCESSION   Circular
KEYWORDS    .
SOURCE       (CN5041_pAAV-TelN-LITR-eHGT_1417m-minBG-SYFP2-P2A-3XFLAG-10aa-H2B*-WPRE-BGHpA-RITR-TelN)
  ORGANISM  .
FEATURES             Location/Qualifiers
     LTR             complement(1..130)
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ITR"
                     /Transferred_Similarity="92.20%"
                     /Primary_Match="92.20%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1cu-gl1nwdj"">ITR</a>"
                     /label="ITR"
     Enhancer        144..710
                     /X-Transferred_From="AiE1417m"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:12ap-hkqy0ct"">AiE1417m</a
                     >"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AiE1417m"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:12ap-hkqy0ct"">AiE1417m</a
                     >"
                     /label="AiE1417m"
     promoter        717..769
                     /created_by="yoav.ben-simon"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Beta Globin minimal promoter"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; minBG
                     promoter (<a
                     href=""urn:local:yoav.ben-simon:14q-gl1nvql"">Beta Globin
                     minimal promoter</a>)"
                     /label="minBG promoter"
     regulatory      804..813
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="Kozak sequence"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:19j-gl1nw38"">Kozak
                     sequence</a>"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Kozak sequence"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:19j-gl1nw38"">Kozak
                     sequence</a>"
                     /label="Kozak sequence"
     CDS             810..1529
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SYFP2"
                     /Transferred_Similarity="98.75%"
                     /Primary_Match="98.75%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1cz-gl1nwdj"">SYFP2</a>"
                     /Alternative_Match="97.35%; Adjusted-Interval-0:0/-3; <a
                     href=""urn:local:yoav.ben-simon:14v-gl1nvql"">Cerulean</a>
                     "
                     /Alternative_Match="96.94%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1er-gl1nwjw"">EYFP</a>"
                     /Alternative_Match="96.81%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1aq-gl1nw6h"">mCitrine</a>
                     "
                     /Alternative_Match="96.54%; Adjusted-Interval-0:-3/0; <a
                     href=""urn:local:yoav.ben-simon:163-gl1nvtx"">EGFP</a>"
                     /Alternative_Match="95.56%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1ao-gl1nw6h"">mCerulean3</
                     a>"
                     /Alternative_Match="93.30%; Adjusted-Interval-0:-44/-3; <a
                     href=""urn:local:yoav.ben-simon:1bu-gl1nwa6"">mTurquoise</
                     a>"
                     /label="SYFP2"
     Tag             1605..1670
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="3X FLAG"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:13k-gl1nvn3"">3X FLAG</a>"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="3XFLAG"
                     /Transferred_Similarity="98.48%"
                     /Primary_Match="98.48%; Adjusted-Interval-0:0/0; 3X FLAG
                     (<a
                     href=""urn:local:yoav.ben-simon:13k-gl1nvn3"">3XFLAG</a>)"
                     /label="3X FLAG"
     Linker          1671..1700
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="10 a.a. linker"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:13i-gl1nvn3"">10 a.a.
                     linker</a>"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="10 a.a. linker"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:13i-gl1nvn3"">10 a.a.
                     linker</a>"
                     /label="10 a.a. linker"
     sig_peptide     1701..2075
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="H2B"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:178-gl1nvx6"">H2B</a>"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="H2B"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:178-gl1nvx6"">H2B</a>"
                     /label="H2B"
     3'UTR           2110..2355
                     /created_by="yoav.ben-simon"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="WPRE3"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1e2-gl1nwgr"">WPRE3</a>"
                     /label="WPRE3"
     polyA_signal    2369..2572
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="BGH-pA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:14r-gl1nvql"">BGH-pA</a>"
                     /label="BGH-pA"
     LTR             2601..2741
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ITR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1cu-gl1nwdj"">ITR</a>"
                     /label="ITR"
     misc_feature    2748..2803
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="TelN_seq"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1n1x-gn0w5a7"">TelN_seq</a
                     >"
                     /label="TelN_seq"
     misc_feature    complement(2748..2803)
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="TelN_seq"
                     /Transferred_Similarity="92.86%"
                     /Primary_Match="92.86%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1n1x-gn0w5a7"">TelN_seq</a
                     >"
                     /label="TelN_seq"
     rep_origin      2889..3195
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="f1 Origin"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:170-gl1nvx6"">f1
                     Origin</a>"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="f1 Origin"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:170-gl1nvx6"">f1
                     Origin</a>"
                     /label="f1 Origin"
     promoter        3609..3713
                     /gene="bla"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR promoter"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:vx7-gmqsxiz"">AmpR
                     promoter</a>"
                     /label="AmpR promoter"
     misc_feature    3644..3651
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="FRT3 spacer (FASEBJ 2011, 25, 1)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:174-gl1nvx6"">FRT3 spacer
                     (FASEBJ 2011, 25, 1)</a>"
                     /label="FRT3 spacer (FASEBJ 2011, 25, 1)"
     CDS             3714..4571
                     /created_by="yoav.ben-simon"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:13p-gl1nvn3"">AmpR</a>"
                     /label="AmpR"
     rep_origin      4722..5389
                     /vntifkey=33
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pUC ori"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pUC\ori
                     (<a href=""urn:local:yoav.ben-simon:vxh-gmqt1zl"">pUC
                     ori</a>)"
                     /label="pUC\ori"
     misc_feature    5390..5445
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="TelN_seq"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1n1x-gn0w5a7"">TelN_seq</a
                     >"
                     /label="TelN_seq"
     misc_feature    complement(5390..5445)
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="TelN_seq"
                     /Transferred_Similarity="92.86%"
                     /Primary_Match="92.86%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1n1x-gn0w5a7"">TelN_seq</a
                     >"
                     /label="TelN_seq"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgttgtgca ggaatagaaa ccctgactaa gacactcact
      181 ttcccctctt tgttatcaaa gctgctgcca gccactgggc tcctctcctg tgaccgatct
      241 atagttttca agtgttttct ttgagcaggc ttttgtgtgt gtatagtgtg tgatcagaaa
      301 gattatctta ttccagagga catgtgtgtg cttaacctga tatcttttcc caggaagcta
      361 gactcctctg cctagggatg ttcaacaccc ctcctgaggc ccggtccatg ctccccgctt
      421 taatgtgttc agaaaccctc agctcagcac caggcactct ccaggcactg ctcttatcct
      481 cacttcctga gattgagcac agtgaacttg ctgagccagc agcctgggga tgctctgctc
      541 tctctggaag tgcttttggg cttaaagaat ggtttctgca gctttgtgag cagaagccac
      601 acagcctttt cttctcccct ggaccacagg atttactggc acgcacaagg acggtcacaa
      661 aggtcgccca cccctgcccc caagctttag tgatttatat tcccaagtca gagctcgggc
      721 tgggcataaa agtcagggca gagccatcta ttgcttacat ttgcttctgg gatccagatc
      781 tttcgaagct agcgctaccg gtcgccacca tggtctccaa gggcgaggag ctgttcaccg
      841 gggtggtgcc catcctggtc gagctggacg gcgatgtcaa cggccacaag ttcagcgtgt
      901 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagctg atctgcacca
      961 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgggctac ggcgtgcagt
     1021 gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     1081 aaggctacgt ccaggagcgc accatcttct tcaaagacga cggcaactac aagacccgcg
     1141 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     1201 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     1261 tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccgccaca
     1321 acatcgagga cggcggcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     1381 acggccccgt gctgctgccc gacaaccact acctgagcta ccagtccaag ctgagcaaag
     1441 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     1501 ctctcggcat ggacgagctg tacaagggct ctggtgctac caacttctca ctgttgaaac
     1561 aggcagggga tgtagaggag aatccagggc ctggtgctag tggagactac aaagaccatg
     1621 acggagatta taaagatcat gacatcgatt acaaggatga cgatgacaag tccggactca
     1681 gatctggagg ctccggaggc ccagagccag cgaagtctgc tcccgccccg aaaaagggct
     1741 ccaagaaggc ggtgactaag gcgcagaaga aaggcggcaa gaagcgcaag cgcagccgca
     1801 aggagagcta ttccatctat gtgtataagg ttctgaagca ggtccaccct gacaccggca
     1861 tttcgtccaa ggccatgggc atcatgaact cgtttgtgaa cgacattttc gagcgcatcg
     1921 caggtgaggc ttcccgcctg gcgcattaca acaagcgctc gaccatcacc tccagggaga
     1981 tccagacggc cgtgcgcctg ctgctgcctg gggagttggc caagcacgcc gtgtccgagg
     2041 gtactaaggc catcaccaag tacaccagcg ctaagtaatg aggcgcgccg cggccgcgaa
     2101 ttcgatatca taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     2161 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     2221 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggtta gttcttgcca
     2281 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     2341 ctgacaattc cgtggctcga gagatcttcg actgtgcctt ctagttgcca gccatctgtt
     2401 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     2461 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     2521 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgagatctca
     2581 cgtgcggacc gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg
     2641 cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg
     2701 ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctat cagcacacaa
     2761 ttgcccatta tacgcgcgta taatggacta ttgtgtgctg atatgatgcg gtattttctc
     2821 cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct
     2881 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     2941 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     3001 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     3061 ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct
     3121 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     3181 tccaaactgg aacaacactc aaccctatct cgggctattc ttttgattta taagggattt
     3241 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     3301 ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg
     3361 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     3421 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     3481 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     3541 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     3601 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     3661 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     3721 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     3781 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     3841 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     3901 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     3961 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     4021 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     4081 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     4141 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     4201 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     4261 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     4321 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     4381 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     4441 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     4501 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     4561 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     4621 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     4681 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     4741 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     4801 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     4861 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     4921 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     4981 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     5041 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     5101 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     5161 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     5221 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     5281 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     5341 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcat atcagcacac
     5401 aattgcccat tatacgcgcg tataatggac tattgtgtgc tgataacatg t
//
