LOCUS       AiP15260                4954 bp    DNA     circular UNA 04-JUN-2024
DEFINITION  pAAV-AiE0779m_3xC2-minBG-iCre(R297T)-3XmiR126TS-BGHpa.
ACCESSION   urn.local.yoav.ben-simon.2dj-hy1s1bn
VERSION     urn.local.yoav.ben-simon.2dj-hy1s1bn
KEYWORDS    .
SOURCE       (pAAV-3xcore2_eHGT_779m-minBG-iCre(R297T)-3XmiR126TS-BGHpA)
  ORGANISM  .
FEATURES             Location/Qualifiers
     LTR             complement(1..141)
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ITR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1cu-gl1nwdj"">ITR</a>"
                     /label="ITR"
     Bashed_enhancer 156..737
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="AiE0779m_3xC2"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:zzz-hkqxi53"">AiE0779m_3xC
                     2</a>"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AiE0779m_3xC2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:zzz-hkqxi53"">AiE0779m_3xC
                     2</a>"
                     /Alternative_Match="97.74%; Adjusted-Interval-0:-12/71; <a
                     href=""urn:local:yoav.ben-simon:100x-hkqxidu"">AiE0779m_6x
                     C2</a>"
                     /label="AiE0779m_3xC2"
     promoter        744..796
                     /created_by="yoav.ben-simon"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Beta Globin minimal promoter"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; minBG
                     promoter (<a
                     href=""urn:local:yoav.ben-simon:14q-gl1nvql"">Beta Globin
                     minimal promoter</a>)"
                     /label="minBG promoter"
     regulatory      819..828
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="Kozak sequence"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:19j-gl1nw38"">Kozak
                     sequence</a>"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Kozak sequence"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:19j-gl1nw38"">Kozak
                     sequence</a>"
                     /label="Kozak sequence"
     CDS             825..1880
                     /vntifkey=21
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="iCre(297T)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:vxt-gmqtbht"">iCre(297T)</
                     a>"
                     /Alternative_Match="99.62%; Adjusted-Interval-0:-3/0; <a
                     href=""urn:local:yoav.ben-simon:18d-gl1nw07"">iCre</a>"
                     /label="iCre(R297T)"
     polyA_signal    1991..2194
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="BGH-pA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:14r-gl1nvql"">BGH-pA</a>"
                     /label="BGH-pA"
     LTR             2217..2357
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ITR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1cu-gl1nwdj"">ITR</a>"
                     /label="ITR"
     rep_origin      2449..2755
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="f1 Origin"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:170-gl1nvx6"">f1
                     Origin</a>"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="f1 Origin"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:170-gl1nvx6"">f1
                     Origin</a>"
                     /label="f1 Origin"
     promoter        3169..3273
                     /gene="bla"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR promoter"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:vx7-gmqsxiz"">AmpR
                     promoter</a>"
                     /label="AmpR promoter"
     misc_feature    3204..3211
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="FRT3 spacer (FASEBJ 2011, 25, 1)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:174-gl1nvx6"">FRT3 spacer
                     (FASEBJ 2011, 25, 1)</a>"
                     /label="FRT3 spacer (FASEBJ 2011, 25, 1)"
     CDS             3274..4131
                     /created_by="yoav.ben-simon"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:13p-gl1nvn3"">AmpR</a>"
                     /label="AmpR"
     rep_origin      4282..4949
                     /vntifkey=33
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pUC ori"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pUC\ori
                     (<a href=""urn:local:yoav.ben-simon:vxh-gmqt1zl"">pUC
                     ori</a>)"
                     /label="pUC\ori"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtaagtt tgagacaaat taaggaaaag
      181 cgggttggga ggagtctgag gctgtgggtg gctgcctcga gaggctgctc acaacctgac
      241 atgtttcaga tcttttgaca cggtcatgca agcaacagaa acttatatgg tttttagtct
      301 cccaggtagc agggaaatga tgatgtggga ggattagggg ggggaaaata agtttgagac
      361 aaattaagga aaagcgggtt gggaggagtc tgaggctgtg ggtggctgcc tcgagaggct
      421 gctcacaacc tgacatgttt cagatctttt gacacggtca tgcaagcaac agaaacttat
      481 atggttttta gtctcccagg tagcagggaa atgatgatgt gggaggatta ggggggggaa
      541 aataagtttg agacaaatta aggaaaagcg ggttgggagg agtctgaggc tgtgggtggc
      601 tgcctcgaga ggctgctcac aacctgacat gtttcagatc ttttgacacg gtcatgcaag
      661 caacagaaac ttatatggtt tttagtctcc caggtagcag ggaaatgatg atgtgggagg
      721 attagggggg ggaaaatgag ctcgggctgg gcataaaagt cagggcagag ccatctattg
      781 cttacatttg cttctgggat ccagatcttt cgaagctagc caccatggtg cccaagaaga
      841 agaggaaagt ctccaacctg ctgactgtgc accaaaacct gcctgccctc cctgtggatg
      901 ccacctctga tgaagtcagg aagaacctga tggacatgtt cagggacagg caggccttct
      961 ctgaacacac ctggaagatg ctcctgtctg tgtgcagatc ctgggctgcc tggtgcaagc
     1021 tgaacaacag gaaatggttc cctgctgaac ctgaggatgt gagggactac ctcctgtacc
     1081 tgcaagccag aggcctggct gtgaagacca tccaacagca cctgggccag ctcaacatgc
     1141 tgcacaggag atctggcctg cctcgccctt ctgactccaa tgctgtgtcc ctggtgatga
     1201 ggagaatcag aaaggagaat gtggatgctg gggagagagc caagcaggcc ctggcctttg
     1261 aacgcactga ctttgaccaa gtcagatccc tgatggagaa ctctgacaga tgccaggaca
     1321 tcaggaacct ggccttcctg ggcattgcct acaacaccct gctgcgcatt gccgaaattg
     1381 ccagaatcag agtgaaggac atctcccgca ccgatggtgg gagaatgctg atccacattg
     1441 gcaggaccaa gaccctggtg tccacagctg gtgtggagaa ggccctgtcc ctgggggtta
     1501 ccaagctggt ggagagatgg atctctgtgt ctggtgtggc tgatgacccc aacaactacc
     1561 tgttctgccg ggtcagaaag aatggtgtgg ctgccccttc tgccacctcc caactgtcca
     1621 cccgggccct ggaagggatc tttgaggcca cccaccgcct gatctatggt gccaaggatg
     1681 actctgggca gagatacctg gcctggtctg gccactctgc cagagtgggt gctgccaccg
     1741 acatggccag ggctggtgtg tccatccctg aaatcatgca ggctggtggc tggaccaatg
     1801 tgaacattgt gatgaactac atcagaaacc tggactctga gactggggcc atggtgaggc
     1861 tgctcgaaga tggggactga ggcgcgcccg cgtaccaaaa gtaataatga ggtggacgcg
     1921 taccaaaagt aataatgaat gcatcgcgta ccaaaagtaa taatggaatt caagcttctc
     1981 gagagatctt cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     2041 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     2101 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     2161 aagggggagg attgggaaga caatagcagg catgcacgtg cggaccgagc ggccgcagga
     2221 acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg
     2281 gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc
     2341 gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     2401 ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg
     2461 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     2521 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     2581 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     2641 cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     2701 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     2761 aaccctatct cgggctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     2821 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt
     2881 acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
     2941 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     3001 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     3061 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     3121 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     3181 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     3241 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     3301 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     3361 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     3421 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     3481 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     3541 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     3601 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     3661 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     3721 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     3781 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     3841 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     3901 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     3961 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     4021 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     4081 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     4141 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     4201 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     4261 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     4321 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     4381 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     4441 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     4501 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     4561 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     4621 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     4681 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     4741 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     4801 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     4861 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     4921 ttcctggcct tttgctggcc ttttgctcac atgt
//
