LOCUS       AiP15357                5929 bp    DNA     circular UNA 08-MAY-2024
DEFINITION  pAAV-AiE0441h_3xC2-minBG-DIO-CoChR-EGFP-WPRE3-BGHpa.
ACCESSION   urn.local.yoav.ben-simon.2dm-hy1s1bo
VERSION     urn.local.yoav.ben-simon.2dm-hy1s1bo
KEYWORDS    .
SOURCE       (pAAV-AiE0441h_3xC2-minBG-DIO-CoChR-EGFP-WPRE3-BGHpA)
  ORGANISM  .
FEATURES             Location/Qualifiers
     LTR             complement(1..141)
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ITR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1cu-gl1nwdj"">ITR</a>"
                     /label="ITR"
     Bashed_enhancer 156..752
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="AiE0441h_3xC2"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:yg0-hkqx8kg"">AiE0441h_3xC
                     2</a>"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AiE0441h_3xC2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:yg0-hkqx8kg"">AiE0441h_3xC
                     2</a>"
                     /label="AiE0441h_3xC2"
     promoter        759..811
                     /created_by="yoav.ben-simon"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Beta Globin minimal promoter"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; minBG
                     promoter (<a
                     href=""urn:local:yoav.ben-simon:14q-gl1nvql"">Beta Globin
                     minimal promoter</a>)"
                     /label="minBG promoter"
     protein_bind    836..869
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="lox2272"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1aj-gl1nw6h"">lox2272</a>"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="lox2272"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1aj-gl1nw6h"">lox2272</a>"
                     /label="lox2272"
     protein_bind    920..953
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="loxP"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1am-gl1nw6h"">loxP</a>"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="loxP"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1am-gl1nw6h"">loxP</a>"
                     /label="loxP"
     CDS             complement(962..1682)
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="EGFP"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:163-gl1nvtx"">EGFP</a>"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="EGFP"
                     /Transferred_Similarity="99.45%"
                     /Primary_Match="99.45%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:163-gl1nvtx"">EGFP</a>"
                     /Alternative_Match="98.19%; Adjusted-Interval-0:-1/0; <a
                     href=""urn:local:yoav.ben-simon:1er-gl1nwjw"">EYFP</a>"
                     /Alternative_Match="97.36%; Adjusted-Interval-0:-1/0; <a
                     href=""urn:local:yoav.ben-simon:1aq-gl1nw6h"">mCitrine</a>
                     "
                     /Alternative_Match="97.35%; Adjusted-Interval-0:-1/3; <a
                     href=""urn:local:yoav.ben-simon:14v-gl1nvql"">Cerulean</a>
                     "
                     /Alternative_Match="97.22%; Adjusted-Interval-0:-1/0; <a
                     href=""urn:local:yoav.ben-simon:1cz-gl1nwdj"">SYFP2</a>"
                     /Alternative_Match="95.69%; Adjusted-Interval-0:-1/0; <a
                     href=""urn:local:yoav.ben-simon:1ao-gl1nw6h"">mCerulean3</
                     a>"
                     /label="EGFP"
     CDS             complement(1697..2560)
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="CoChR"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:15w-gl1nvtw"">CoChR</a>"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="CoChR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:15w-gl1nvtw"">CoChR</a>"
                     /label="CoChR"
     protein_bind    complement(2571..2604)
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="lox2272"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1aj-gl1nw6h"">lox2272</a>"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="lox2272"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1aj-gl1nw6h"">lox2272</a>"
                     /label="lox2272"
     protein_bind    complement(2655..2688)
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="loxP"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1am-gl1nw6h"">loxP</a>"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="loxP"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1am-gl1nw6h"">loxP</a>"
                     /label="loxP"
     3'UTR           2701..2946
                     /created_by="yoav.ben-simon"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="WPRE3"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1e2-gl1nwgr"">WPRE3</a>"
                     /label="WPRE3"
     polyA_signal    2960..3163
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="BGH-pA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:14r-gl1nvql"">BGH-pA</a>"
                     /label="BGH-pA"
     LTR             3192..3332
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ITR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1cu-gl1nwdj"">ITR</a>"
                     /label="ITR"
     rep_origin      3424..3730
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="f1 Origin"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:170-gl1nvx6"">f1
                     Origin</a>"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="f1 Origin"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:170-gl1nvx6"">f1
                     Origin</a>"
                     /label="f1 Origin"
     promoter        4144..4248
                     /gene="bla"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR promoter"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:vx7-gmqsxiz"">AmpR
                     promoter</a>"
                     /label="AmpR promoter"
     misc_feature    4179..4186
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="FRT3 spacer (FASEBJ 2011, 25, 1)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:174-gl1nvx6"">FRT3 spacer
                     (FASEBJ 2011, 25, 1)</a>"
                     /label="FRT3 spacer (FASEBJ 2011, 25, 1)"
     CDS             4249..5106
                     /created_by="yoav.ben-simon"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:13p-gl1nvn3"">AmpR</a>"
                     /label="AmpR"
     rep_origin      5257..5924
                     /vntifkey=33
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pUC ori"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pUC\ori
                     (<a href=""urn:local:yoav.ben-simon:vxh-gmqt1zl"">pUC
                     ori</a>)"
                     /label="pUC\ori"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtcccac acagccccca ccacctggcc
      181 gcccacctga aaaacttttc cccaagttct tgagaagtcg cgtaggatcc aggagagaaa
      241 aatcaaagta ataaaacctc atttaattcc taacggaggg cagagctgcg agctgcaatt
      301 atcttaatgc tgagctattt ttactctctc tccatctctg tcttctgcct ttctcccaca
      361 cagcccccac cacctggccg cccacctgaa aaacttttcc ccaagttctt gagaagtcgc
      421 gtaggatcca ggagagaaaa atcaaagtaa taaaacctca tttaattcct aacggagggc
      481 agagctgcga gctgcaatta tcttaatgct gagctatttt tactctctct ccatctctgt
      541 cttctgcctt tctcccacac agcccccacc acctggccgc ccacctgaaa aacttttccc
      601 caagttcttg agaagtcgcg taggatccag gagagaaaaa tcaaagtaat aaaacctcat
      661 ttaattccta acggagggca gagctgcgag ctgcaattat cttaatgctg agctattttt
      721 actctctctc catctctgtc ttctgccttt ctgagctcgg gctgggcata aaagtcaggg
      781 cagagccatc tattgcttac atttgcttct gggatcctct agagtcgact ccggaataac
      841 ttcgtatagg atactttata cgaagttatg cagaatggta gctggattgt agctgctatt
      901 agcaatatga aacctcttaa taacttcgta tagcatacat tatacgaagt tatggcgcgc
      961 cttacttgta cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca
     1021 ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag ggcggactgg gtgctcaggt
     1081 agtggttgtc gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt
     1141 cggcgagctg cacgctgccg tcctcgatgt tgtggcggat cttgaagttc accttgatgc
     1201 cgttcttctg cttgtcggcc atgatataga cgttgtggct gttgtagttg tactccagct
     1261 tgtgccccag gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca
     1321 ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga
     1381 agaagatggt gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct
     1441 gcttcatgtg gtcggggtag cggctgaagc actgcacgcc gtaggtcagg gtggtcacga
     1501 gggtgggcca gggcacgggc agcttgccgg tggtgcaaat gaacttcagg gtcagcttgc
     1561 cgtaggtggc atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc
     1621 cgtccagctc gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctcactg
     1681 ctactaccgg tgccgccact gtctcctcgt cctcctggtc caccatggtc tccacttcca
     1741 tctcctcgcc ggccacgttg atcttggtct tcttccggat gtcgccgtag atgatgatgt
     1801 gctggtggat cagcactctc agatagtgtc ccagcaggcc ccaacaattc ttgctcatga
     1861 ggtcgatgat ggtgtggcca atggtagagc cgtacacgct gatggcgtcg aatccttctg
     1921 gtcccacgac gaacagcacg gggaacatgc cccaagacag gaagaacagc caagccatga
     1981 tccgcacgac ggttctaggt ctgccctttg gcaccacgtg gtagctctcg atatacacct
     2041 tggcggcgtg gaagaaggtg ttggcgccgt agatgcaacc cagcacgaag aagatgacct
     2101 tgacgtagcc tgtgctcatg gcgcttgtag ctccccacac gatggttccc acgtctgaca
     2161 ccagcagcct catggtccgc ttgctgtagt cgtccttcag gccggtcagg ttgctcaggt
     2221 ggatcaggat gacgggacaa gtcagcagcc actctgcgta tctcagccac tggactcggt
     2281 gtccgttagc caggtacagc atgctggggt cgtcgaactc gtggaagaac tcgatgatga
     2341 ccttggtcag ctcgacgcaa cagacgtaga cctcctccca accgcaagtg gctctccaag
     2401 tctggtaggc gtagaacatc aggatcagga tgctgaagcc gaaggcgaac cactggagga
     2461 tgttggccac cagctgctct gtatcgcttc ccaggctgtc ggtccaagcc aggcaaaagc
     2521 actggtcgat aggcacaatg gcgctgccgt ttcccagcat ggtggctagc ataacttcgt
     2581 ataaagtatc ctatacgaag ttatttgcct taacccagaa attatcactg ttattcttta
     2641 gaatggtgca aagaataact tcgtataatg tatgctatac gaagttatga attcgatatc
     2701 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     2761 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     2821 gtatggcttt cattttctcc tccttgtata aatcctggtt agttcttgcc acggcggaac
     2881 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     2941 ccgtggctcg agagatcttc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     3001 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     3061 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     3121 caggacagca agggggagga ttgggaagac aatagcaggc atgagatctc acgtgcggac
     3181 cgagcggccg caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc
     3241 gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc
     3301 agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt ttctccttac
     3361 gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc
     3421 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     3481 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     3541 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     3601 ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag
     3661 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     3721 actggaacaa cactcaaccc tatctcgggc tattcttttg atttataagg gattttgccg
     3781 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     3841 aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca
     3901 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     3961 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     4021 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
     4081 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     4141 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     4201 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     4261 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     4321 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     4381 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     4441 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     4501 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     4561 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     4621 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     4681 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     4741 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     4801 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     4861 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     4921 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     4981 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     5041 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     5101 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     5161 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     5221 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     5281 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     5341 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     5401 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     5461 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     5521 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     5581 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     5641 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     5701 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     5761 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     5821 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     5881 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgt
//
