LOCUS       AiP15457                5663 bp    DNA     circular UNA 08-MAY-2024
DEFINITION  pAAV-AiE0441h_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpa.
ACCESSION   urn.local.yoav.ben-simon.2dn-hy1s1bo
VERSION     urn.local.yoav.ben-simon.2dn-hy1s1bo
KEYWORDS    .
SOURCE       (pAAV-eHGT_441h_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA)
  ORGANISM  .
FEATURES             Location/Qualifiers
     LTR             complement(1..141)
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ITR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1cu-gl1nwdj"">ITR</a>"
                     /label="ITR"
     Bashed_enhancer 156..752
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="AiE0441h_3xC2"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:yg0-hkqx8kg"">AiE0441h_3xC
                     2</a>"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AiE0441h_3xC2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:yg0-hkqx8kg"">AiE0441h_3xC
                     2</a>"
                     /label="AiE0441h_3xC2"
     promoter        759..811
                     /created_by="yoav.ben-simon"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Beta Globin minimal promoter"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; minBG
                     promoter (<a
                     href=""urn:local:yoav.ben-simon:14q-gl1nvql"">Beta Globin
                     minimal promoter</a>)"
                     /label="minBG promoter"
     CDS             824..1687
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="CoChR"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:15w-gl1nvtw"">CoChR</a>"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="CoChR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:15w-gl1nvtw"">CoChR</a>"
                     /label="CoChR"
     CDS             1702..2422
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="EGFP"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:163-gl1nvtx"">EGFP</a>"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="EGFP"
                     /Transferred_Similarity="99.45%"
                     /Primary_Match="99.45%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:163-gl1nvtx"">EGFP</a>"
                     /Alternative_Match="98.19%; Adjusted-Interval-0:1/0; <a
                     href=""urn:local:yoav.ben-simon:1er-gl1nwjw"">EYFP</a>"
                     /Alternative_Match="97.36%; Adjusted-Interval-0:1/0; <a
                     href=""urn:local:yoav.ben-simon:1aq-gl1nw6h"">mCitrine</a>
                     "
                     /Alternative_Match="97.35%; Adjusted-Interval-0:1/-3; <a
                     href=""urn:local:yoav.ben-simon:14v-gl1nvql"">Cerulean</a>
                     "
                     /Alternative_Match="97.22%; Adjusted-Interval-0:1/0; <a
                     href=""urn:local:yoav.ben-simon:1cz-gl1nwdj"">SYFP2</a>"
                     /Alternative_Match="95.69%; Adjusted-Interval-0:1/0; <a
                     href=""urn:local:yoav.ben-simon:1ao-gl1nw6h"">mCerulean3</
                     a>"
                     /label="EGFP"
     3'UTR           2435..2680
                     /created_by="yoav.ben-simon"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="WPRE3"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1e2-gl1nwgr"">WPRE3</a>"
                     /label="WPRE3"
     polyA_signal    2694..2897
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="BGH-pA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:14r-gl1nvql"">BGH-pA</a>"
                     /label="BGH-pA"
     LTR             2926..3066
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ITR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1cu-gl1nwdj"">ITR</a>"
                     /label="ITR"
     rep_origin      3158..3464
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="f1 Origin"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:170-gl1nvx6"">f1
                     Origin</a>"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="f1 Origin"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:170-gl1nvx6"">f1
                     Origin</a>"
                     /label="f1 Origin"
     promoter        3878..3982
                     /gene="bla"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR promoter"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:vx7-gmqsxiz"">AmpR
                     promoter</a>"
                     /label="AmpR promoter"
     misc_feature    3913..3920
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="FRT3 spacer (FASEBJ 2011, 25, 1)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:174-gl1nvx6"">FRT3 spacer
                     (FASEBJ 2011, 25, 1)</a>"
                     /label="FRT3 spacer (FASEBJ 2011, 25, 1)"
     CDS             3983..4840
                     /created_by="yoav.ben-simon"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:13p-gl1nvn3"">AmpR</a>"
                     /label="AmpR"
     rep_origin      4991..5658
                     /vntifkey=33
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pUC ori"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pUC\ori
                     (<a href=""urn:local:yoav.ben-simon:vxh-gmqt1zl"">pUC
                     ori</a>)"
                     /label="pUC\ori"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtcccac acagccccca ccacctggcc
      181 gcccacctga aaaacttttc cccaagttct tgagaagtcg cgtaggatcc aggagagaaa
      241 aatcaaagta ataaaacctc atttaattcc taacggaggg cagagctgcg agctgcaatt
      301 atcttaatgc tgagctattt ttactctctc tccatctctg tcttctgcct ttctcccaca
      361 cagcccccac cacctggccg cccacctgaa aaacttttcc ccaagttctt gagaagtcgc
      421 gtaggatcca ggagagaaaa atcaaagtaa taaaacctca tttaattcct aacggagggc
      481 agagctgcga gctgcaatta tcttaatgct gagctatttt tactctctct ccatctctgt
      541 cttctgcctt tctcccacac agcccccacc acctggccgc ccacctgaaa aacttttccc
      601 caagttcttg agaagtcgcg taggatccag gagagaaaaa tcaaagtaat aaaacctcat
      661 ttaattccta acggagggca gagctgcgag ctgcaattat cttaatgctg agctattttt
      721 actctctctc catctctgtc ttctgccttt ctgagctcgg gctgggcata aaagtcaggg
      781 cagagccatc tattgcttac atttgcttct gggatccgcc accatgctgg gaaacggcag
      841 cgccattgtg cctatcgacc agtgcttttg cctggcttgg accgacagcc tgggaagcga
      901 tacagagcag ctggtggcca acatcctcca gtggttcgcc ttcggcttca gcatcctgat
      961 cctgatgttc tacgcctacc agacttggag agccacttgc ggttgggagg aggtctacgt
     1021 ctgttgcgtc gagctgacca aggtcatcat cgagttcttc cacgagttcg acgaccccag
     1081 catgctgtac ctggctaacg gacaccgagt ccagtggctg agatacgcag agtggctgct
     1141 gacttgtccc gtcatcctga tccacctgag caacctgacc ggcctgaagg acgactacag
     1201 caagcggacc atgaggctgc tggtgtcaga cgtgggaacc atcgtgtggg gagctacaag
     1261 cgccatgagc acaggctacg tcaaggtcat cttcttcgtg ctgggttgca tctacggcgc
     1321 caacaccttc ttccacgccg ccaaggtgta tatcgagagc taccacgtgg tgccaaaggg
     1381 cagacctaga accgtcgtgc ggatcatggc ttggctgttc ttcctgtctt ggggcatgtt
     1441 ccccgtgctg ttcgtcgtgg gaccagaagg attcgacgcc atcagcgtgt acggctctac
     1501 cattggccac accatcatcg acctcatgag caagaattgt tggggcctgc tgggacacta
     1561 tctgagagtg ctgatccacc agcacatcat catctacggc gacatccgga agaagaccaa
     1621 gatcaacgtg gccggcgagg agatggaagt ggagaccatg gtggaccagg aggacgagga
     1681 gacagtggcg gcaccggtag tagcagtgag caagggcgag gagctgttca ccggggtggt
     1741 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     1801 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatttgca ccaccggcaa
     1861 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     1921 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     1981 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     2041 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     2101 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     2161 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     2221 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     2281 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
     2341 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     2401 catggacgag ctgtacaagt aagaattcga tatcataatc aacctctgga ttacaaaatt
     2461 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     2521 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     2581 tataaatcct ggttagttct tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     2641 tggacagggg ctcggctgtt gggcactgac aattccgtgg ctcgagagat cttcgactgt
     2701 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     2761 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     2821 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     2881 agacaatagc aggcatgaga tctcacgtgc ggaccgagcg gccgcaggaa cccctagtga
     2941 tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg
     3001 tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc
     3061 tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     3121 tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     3181 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     3241 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     3301 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg
     3361 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     3421 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     3481 gggctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     3541 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg
     3601 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
     3661 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     3721 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     3781 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     3841 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     3901 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     3961 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     4021 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     4081 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     4141 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     4201 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     4261 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     4321 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     4381 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     4441 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     4501 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     4561 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     4621 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     4681 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     4741 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     4801 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     4861 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     4921 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     4981 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     5041 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     5101 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     5161 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     5221 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     5281 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     5341 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     5401 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     5461 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     5521 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     5581 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     5641 ttgctggcct tttgctcaca tgt
//
