LOCUS       AiP15508                4800 bp    DNA     circular UNA 04-JUN-2024
DEFINITION  pAAV-AiE1419m-minBG-iCre(R297T)-BGHpa.
ACCESSION   urn.local.yoav.ben-simon.2do-hy1s1bo
VERSION     urn.local.yoav.ben-simon.2do-hy1s1bo
KEYWORDS    .
SOURCE       (pAAV-AiE1419m-minBG-iCre(R297T)-BGHpA)
  ORGANISM  .
FEATURES             Location/Qualifiers
     LTR             complement(1..141)
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ITR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1cu-gl1nwdj"">ITR</a>"
                     /label="ITR"
     Enhancer        156..660
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="AiE1419m"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:12ar-hkqy0ct"">AiE1419m</a
                     >"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AiE1419m"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:12ar-hkqy0ct"">AiE1419m</a
                     >"
                     /label="AiE1419m"
     promoter        667..719
                     /created_by="yoav.ben-simon"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Beta Globin minimal promoter"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; minBG
                     promoter (<a
                     href=""urn:local:yoav.ben-simon:14q-gl1nvql"">Beta Globin
                     minimal promoter</a>)"
                     /label="minBG promoter"
     regulatory      742..751
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="Kozak sequence"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:19j-gl1nw38"">Kozak
                     sequence</a>"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Kozak sequence"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:19j-gl1nw38"">Kozak
                     sequence</a>"
                     /label="Kozak sequence"
     CDS             748..1803
                     /vntifkey=21
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="iCre(297T)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:vxt-gmqtbht"">iCre(297T)</
                     a>"
                     /Alternative_Match="99.62%; Adjusted-Interval-0:-3/0; <a
                     href=""urn:local:yoav.ben-simon:18d-gl1nw07"">iCre</a>"
                     /label="iCre(R297T)"
     polyA_signal    1837..2040
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="BGH-pA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:14r-gl1nvql"">BGH-pA</a>"
                     /label="BGH-pA"
     LTR             2063..2203
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ITR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1cu-gl1nwdj"">ITR</a>"
                     /label="ITR"
     rep_origin      2295..2601
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="f1 Origin"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:170-gl1nvx6"">f1
                     Origin</a>"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="f1 Origin"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:170-gl1nvx6"">f1
                     Origin</a>"
                     /label="f1 Origin"
     promoter        3015..3119
                     /gene="bla"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR promoter"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:vx7-gmqsxiz"">AmpR
                     promoter</a>"
                     /label="AmpR promoter"
     misc_feature    3050..3057
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="FRT3 spacer (FASEBJ 2011, 25, 1)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:174-gl1nvx6"">FRT3 spacer
                     (FASEBJ 2011, 25, 1)</a>"
                     /label="FRT3 spacer (FASEBJ 2011, 25, 1)"
     CDS             3120..3977
                     /created_by="yoav.ben-simon"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:13p-gl1nvn3"">AmpR</a>"
                     /label="AmpR"
     rep_origin      4128..4795
                     /vntifkey=33
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pUC ori"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pUC\ori
                     (<a href=""urn:local:yoav.ben-simon:vxh-gmqt1zl"">pUC
                     ori</a>)"
                     /label="pUC\ori"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtaactt gacagtggtg ggcaaaagta
      181 aaacttgaaa gacaaaactt ggaagtgctg gaactgtaac tccagaggtc tctgagagag
      241 cctggcttcg agaactcttt aactccctct tctcagcctc tgagcagcaa acccagcaca
      301 gctgttctcg ctgtaaaggg cagctatgca gggcagagtt cccagaaggc ttctatgctc
      361 tcaaatccgc tctataaagt aggaagtgtg ctgagtacca ggccatccat actgacaacc
      421 acagcagatc caccgccaag catttttatt gttgcggcag atggtggcct gattgtgaag
      481 cgtcccagag caagggtgcc cttcaacatt ggaaattaat agctaagcta ctcatctccc
      541 ttcaaatcag aggcaatgtc aggctccgag acaaccttca gggtagttta actgagtccg
      601 gaagctgcca gagcaggacc catgtgggcc agccactgtc cctctttctg tagctgactg
      661 gagctcgggc tgggcataaa agtcagggca gagccatcta ttgcttacat ttgcttctgg
      721 gatccagatc tttccaagct agccaccatg gtgcccaaga agaagaggaa agtctccaac
      781 ctgctgactg tgcaccaaaa cctgcctgcc ctccctgtgg atgccacctc tgatgaagtc
      841 aggaagaacc tgatggacat gttcagggac aggcaggcct tctctgaaca cacctggaag
      901 atgctcctgt ctgtgtgcag atcctgggct gcctggtgca agctgaacaa caggaaatgg
      961 ttccctgctg aacctgagga tgtgagggac tacctcctgt acctgcaagc cagaggcctg
     1021 gctgtgaaga ccatccaaca gcacctgggc cagctcaaca tgctgcacag gagatctggc
     1081 ctgcctcgcc cttctgactc caatgctgtg tccctggtga tgaggagaat cagaaaggag
     1141 aatgtggatg ctggggagag agccaagcag gccctggcct ttgaacgcac tgactttgac
     1201 caagtcagat ccctgatgga gaactctgac agatgccagg acatcaggaa cctggccttc
     1261 ctgggcattg cctacaacac cctgctgcgc attgccgaaa ttgccagaat cagagtgaag
     1321 gacatctccc gcaccgatgg tgggagaatg ctgatccaca ttggcaggac caagaccctg
     1381 gtgtccacag ctggtgtgga gaaggccctg tccctggggg ttaccaagct ggtggagaga
     1441 tggatctctg tgtctggtgt ggctgatgac cccaacaact acctgttctg ccgggtcaga
     1501 aagaatggtg tggctgcccc ttctgccacc tcccaactgt ccacccgggc cctggaaggg
     1561 atctttgagg ccacccaccg cctgatctat ggtgccaagg atgactctgg gcagagatac
     1621 ctggcctggt ctggccactc tgccagagtg ggtgctgcca ccgacatggc cagggctggt
     1681 gtgtccatcc ctgaaatcat gcaggctggt ggctggacca atgtgaacat tgtgatgaac
     1741 tacatcagaa acctggactc tgagactggg gccatggtga ggctgctcga agatggggac
     1801 tgaggcgcgc cgaattcaag cttctcgaga gatcttcgac tgtgccttct agttgccagc
     1861 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     1921 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     1981 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     2041 cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg
     2101 cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc
     2161 cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat
     2221 tttctcctta cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg
     2281 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     2341 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     2401 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     2461 ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat
     2521 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     2581 tcttgttcca aactggaaca acactcaacc ctatctcggg ctattctttt gatttataag
     2641 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     2701 cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct
     2761 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     2821 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     2881 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     2941 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     3001 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     3061 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     3121 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     3181 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     3241 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     3301 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     3361 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     3421 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     3481 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     3541 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     3601 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     3661 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     3721 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     3781 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     3841 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     3901 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     3961 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     4021 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     4081 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     4141 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     4201 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     4261 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     4321 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     4381 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     4441 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     4501 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     4561 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     4621 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     4681 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     4741 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
//
