LOCUS       AiP15579                5600 bp    DNA     circular UNA 08-MAY-2024
DEFINITION  pAAV-AiE0780m-minBG-CoChR-EGFP-WPRE3-BGHpa.
ACCESSION   urn.local.yoav.ben-simon.2dq-hy1s1bo
VERSION     urn.local.yoav.ben-simon.2dq-hy1s1bo
KEYWORDS    .
SOURCE       (pAAV-AiE0780m-minBG-CoChR-EGFP-WPRE3-BGHpA)
  ORGANISM  .
FEATURES             Location/Qualifiers
     LTR             complement(1..141)
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ITR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1cu-gl1nwdj"">ITR</a>"
                     /label="ITR"
     Enhancer        156..689
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="AiE0780m"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:100y-hkqxidu"">AiE0780m</a
                     >"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AiE0780m"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:100y-hkqxidu"">AiE0780m</a
                     >"
                     /label="AiE0780m"
     promoter        696..748
                     /created_by="yoav.ben-simon"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Beta Globin minimal promoter"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; minBG
                     promoter (<a
                     href=""urn:local:yoav.ben-simon:14q-gl1nvql"">Beta Globin
                     minimal promoter</a>)"
                     /label="minBG promoter"
     CDS             761..1624
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="CoChR"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:15w-gl1nvtw"">CoChR</a>"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="CoChR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:15w-gl1nvtw"">CoChR</a>"
                     /label="CoChR"
     CDS             1639..2359
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="EGFP"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:163-gl1nvtx"">EGFP</a>"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="EGFP"
                     /Transferred_Similarity="99.45%"
                     /Primary_Match="99.45%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:163-gl1nvtx"">EGFP</a>"
                     /Alternative_Match="98.19%; Adjusted-Interval-0:1/0; <a
                     href=""urn:local:yoav.ben-simon:1er-gl1nwjw"">EYFP</a>"
                     /Alternative_Match="97.36%; Adjusted-Interval-0:1/0; <a
                     href=""urn:local:yoav.ben-simon:1aq-gl1nw6h"">mCitrine</a>
                     "
                     /Alternative_Match="97.35%; Adjusted-Interval-0:1/-3; <a
                     href=""urn:local:yoav.ben-simon:14v-gl1nvql"">Cerulean</a>
                     "
                     /Alternative_Match="97.22%; Adjusted-Interval-0:1/0; <a
                     href=""urn:local:yoav.ben-simon:1cz-gl1nwdj"">SYFP2</a>"
                     /Alternative_Match="95.69%; Adjusted-Interval-0:1/0; <a
                     href=""urn:local:yoav.ben-simon:1ao-gl1nw6h"">mCerulean3</
                     a>"
                     /label="EGFP"
     3'UTR           2372..2617
                     /created_by="yoav.ben-simon"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="WPRE3"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1e2-gl1nwgr"">WPRE3</a>"
                     /label="WPRE3"
     polyA_signal    2631..2834
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="BGH-pA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:14r-gl1nvql"">BGH-pA</a>"
                     /label="BGH-pA"
     LTR             2863..3003
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ITR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1cu-gl1nwdj"">ITR</a>"
                     /label="ITR"
     rep_origin      3095..3401
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="f1 Origin"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:170-gl1nvx6"">f1
                     Origin</a>"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="f1 Origin"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:170-gl1nvx6"">f1
                     Origin</a>"
                     /label="f1 Origin"
     promoter        3815..3919
                     /gene="bla"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR promoter"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:vx7-gmqsxiz"">AmpR
                     promoter</a>"
                     /label="AmpR promoter"
     misc_feature    3850..3857
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="FRT3 spacer (FASEBJ 2011, 25, 1)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:174-gl1nvx6"">FRT3 spacer
                     (FASEBJ 2011, 25, 1)</a>"
                     /label="FRT3 spacer (FASEBJ 2011, 25, 1)"
     CDS             3920..4777
                     /created_by="yoav.ben-simon"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:13p-gl1nvn3"">AmpR</a>"
                     /label="AmpR"
     rep_origin      4928..5595
                     /vntifkey=33
                     /note="Derived using Geneious Prime 2024.0.5 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pUC ori"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pUC\ori
                     (<a href=""urn:local:yoav.ben-simon:vxh-gmqt1zl"">pUC
                     ori</a>)"
                     /label="pUC\ori"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgttgttt cacaaactac aggttcactg
      181 gagcacagcc atgccaacat ggcatctaag gctggtatga cctcgagacc cctccagcac
      241 tccttcgttc agccatagaa caaggagaca cagataaagt ctggagccgg gcccaggtac
      301 ctgaggctca gagatgagcc cgcagcgaag ctgacaactg actcattcct ccgacactct
      361 cccaccctcc cagctaagga ggtgtctgat gtgaacatgt tctgcctctt tgttgcctgc
      421 tgttgggggc gagggctttg ggtggatttt gaagagcaat tatgcttatt actggttagg
      481 atttctctgt ttgctgagtg cattaacttt tagcgcttcc catcactcat tcttcttcac
      541 accctcttgt cagttacaaa tgtgcaggtg gagagccacg aaaaaaccct gcagcaacgg
      601 caacagaaat cacagcaaca acaacaaatg aacagaaagg atagaaagca gatactcaaa
      661 gatggagaaa aagatactcc cgagaggctg agctcgggct gggcataaaa gtcagggcag
      721 agccatctat tgcttacatt tgcttctggg atccgccacc atgctgggaa acggcagcgc
      781 cattgtgcct atcgaccagt gcttttgcct ggcttggacc gacagcctgg gaagcgatac
      841 agagcagctg gtggccaaca tcctccagtg gttcgccttc ggcttcagca tcctgatcct
      901 gatgttctac gcctaccaga cttggagagc cacttgcggt tgggaggagg tctacgtctg
      961 ttgcgtcgag ctgaccaagg tcatcatcga gttcttccac gagttcgacg accccagcat
     1021 gctgtacctg gctaacggac accgagtcca gtggctgaga tacgcagagt ggctgctgac
     1081 ttgtcccgtc atcctgatcc acctgagcaa cctgaccggc ctgaaggacg actacagcaa
     1141 gcggaccatg aggctgctgg tgtcagacgt gggaaccatc gtgtggggag ctacaagcgc
     1201 catgagcaca ggctacgtca aggtcatctt cttcgtgctg ggttgcatct acggcgccaa
     1261 caccttcttc cacgccgcca aggtgtatat cgagagctac cacgtggtgc caaagggcag
     1321 acctagaacc gtcgtgcgga tcatggcttg gctgttcttc ctgtcttggg gcatgttccc
     1381 cgtgctgttc gtcgtgggac cagaaggatt cgacgccatc agcgtgtacg gctctaccat
     1441 tggccacacc atcatcgacc tcatgagcaa gaattgttgg ggcctgctgg gacactatct
     1501 gagagtgctg atccaccagc acatcatcat ctacggcgac atccggaaga agaccaagat
     1561 caacgtggcc ggcgaggaga tggaagtgga gaccatggtg gaccaggagg acgaggagac
     1621 agtggcggca ccggtagtag cagtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     1681 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     1741 cgagggcgat gccacctacg gcaagctgac cctgaagttc atttgcacca ccggcaagct
     1801 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
     1861 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     1921 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     1981 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     2041 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     2101 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     2161 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     2221 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     2281 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     2341 ggacgagctg tacaagtaag aattcgatat cataatcaac ctctggatta caaaatttgt
     2401 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     2461 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     2521 aaatcctggt tagttcttgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     2581 acaggggctc ggctgttggg cactgacaat tccgtggctc gagagatctt cgactgtgcc
     2641 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     2701 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     2761 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     2821 caatagcagg catgagatct cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg
     2881 agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg
     2941 cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc
     3001 aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac
     3061 gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     3121 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     3181 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     3241 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga
     3301 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     3361 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggg
     3421 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     3481 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg
     3541 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     3601 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     3661 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     3721 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     3781 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     3841 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     3901 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     3961 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     4021 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     4081 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     4141 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     4201 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     4261 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     4321 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     4381 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     4441 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     4501 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     4561 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     4621 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     4681 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     4741 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     4801 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     4861 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     4921 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     4981 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     5041 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     5101 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     5161 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     5221 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     5281 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     5341 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     5401 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     5461 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     5521 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     5581 ctggcctttt gctcacatgt
//
