LOCUS       AiP15819_pAAV-AiE0873m_3XC2-minBG-jGCaMP8s-WPRE3-BGHpA        5666 bp    DNA     linear       26-SEP-2024
DEFINITION  .
ACCESSION   urn.local...8l-h6x68nh
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     ApEinfo:methylated:1                                               
            This file is created by Vector NTI                                 
            http://www.invitrogen.com/                                         
            ORIGDB|GenBank                                                     
            VNTDATE|-14086910|                                                 
            VNTDBDATE|-14086910|                                               
            LSOWNER|                                                           
            VNTNAME|CN3750-pAAV-3xhI56i(core)-minBG-SYFP2-P2A-3XFLAG-10aa-H2B*-
            WPRE3-BGHpA| VNTAUTHORNAME|Jonathan Ting|                          
                         VNTOAUTHORNAME|UNKNOWN|.
FEATURES             Location/Qualifiers
     misc_feature    1..130
                     /locus_tag="L-ITR"
                     /ApEinfo_label="L-ITR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey=21
                     /label="L-ITR"
     misc_feature    145..1053
                     /Original_Bases="GGTACCCTAAATAAAGATGGCTTTTTAGTATTAAAAGTGGA
                     AGAAAATTACAGGTAATTATCTTTGACGGTAAAAACGCTGTAATCAGCGGGCTACATG
                     AAAAATTACTCTAATTATGGCTGCATTTAAGAGAATGGCTAAATAAAGATGGCTTTTT
                     AGTATTAAAAGTGGAAGAAAATTACAGGTAATTATCTTTGACGGTAAAAACGCTGTAA
                     TCAGCGGGCTACATGAAAAATTACTCTAATTATGGCTGCATTTAAGAGAATGGCTAAA
                     TAAAGATGGCTTTTTAGTATTAAAAGTGGAAGAAAATTACAGGTAATTATCTTTGACG
                     GTAAAAACGCTGTAATCAGCGGGCTACATGAAAAATTACTCTAATTATGGCTGCATTT
                     AAGAGAATG"
                     /label="GGTACCCTAAATAAAGATGGCTTTTTAGTATTAAAAGTGGAAGAAAATTA
                     CAGGTAATTATCTTTGACGGTAAAAACGCTGTAATCAGCGGGCTACATGAAAAATTAC
                     TCTAATTATGGCTGCATTTAAGAGAATGGCTAAATAAAGATGGCTTTTTAGTATTAAA
                     AGTGGAAGAAAATTACAGGTAATTATCTTTGACGGTAAAAACGCTGTAATCAGCGGGC
                     TACATGAAAAATTACTCTAATTATGGCTGCATTTAAGAGAATGGCTAAATAAAGATGG
                     CTTTTTAGTATTAAAAGTGGAAGAAAATTACAGGTAATTATCTTTGACGGTAAAAACG
                     CTGTAATCAGCGGGCTACATGAAAAATTACTCTAATTATGGCTGCATTTAAGAGAATG
                     "
                     /note="Geneious type: Editing History Replacement"
     misc_feature    145..1053
                     /created_by="ximena.opitzaraya"
                     /label="3xcore2_eHGT_873m"
     misc_feature    1060..1112
                     /locus_tag="Beta\Globin\minimal\promoter"
                     /ApEinfo_label="Beta\Globin\minimal\promoter"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey=21
                     /label="Beta\Globin\minimal\promoter"
     misc_feature    1101
                     /locus_tag="transcriptional\start"
                     /ApEinfo_label="transcriptional\start"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey=21
                     /label="transcriptional\start"
     misc_feature    1137..2408
                     /Original_Bases="GCTACCGGTCGCCACCATGGTCTCCAAGGGCGAGGAGCTGT
                     TCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGATGTCAACGGCCACAAGTT
                     CAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGCTG
                     ATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGGGCT
                     ACGGCGTGCAGTGCTTCGCCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAA
                     GTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAAGACGACGGC
                     AACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCG
                     AGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTA
                     CAACTACAACAGCCACAACGTCTATATCACCGCCGACAAGCAGAAGAACGGCATCAAG
                     GCCAACTTCAAGATCCGCCACAACATCGAGGACGGCGGCGTGCAGCTCGCCGACCACT
                     ACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCT
                     GAGCTACCAGTCCAAGCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTG
                     CTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGGGCT
                     CTGGTGCTACCAACTTCTCACTGTTGAAACAGGCAGGGGATGTAGAGGAGAATCCAGG
                     GCCTGGTGCTAGTGGAGACTACAAAGACCATGACGGAGATTATAAAGATCATGACATC
                     GATTACAAGGATGACGATGACAAGTCCGGACTCAGATCTGGAGGCTCCGGAGGCCCAG
                     AGCCAGCGAAGTCTGCTCCCGCCCCGAAAAAGGGCTCCAAGAAGGCGGTGACTAAGGC
                     GCAGAAGAAAGGCGGCAAGAAGCGCAAGCGCAGCCGCAAGGAGAGCTATTCCATCTAT
                     GTGTATAAGGTTCTGAAGCAGGTCCACCCTGACACCGGCATTTCGTCCAAGGCCATGG
                     GCATCATGAACTCGTTTGTGAACGACATTTTCGAGCGCATCGCAGGTGAGGCTTCCCG
                     CCTGGCGCATTACAACAAGCGCTCGACCATCACCTCCAGGGAGATCCAGACGGCCGTG
                     CGCCTGCTGCTGCCTGGGGAGTTGGCCAAGCACGCCGTGTCCGAGGGTACTAAGGCCA
                     TCACCAAGTACACCAGCGCTAAGTAATG"
                     /label="GCTACCGGTCGCCACCATGGTCTCCAAGGGCGAGGAGCTGTTCACCGGGG
                     TGGTGCCCATCCTGGTCGAGCTGGACGGCGATGTCAACGGCCACAAGTTCAGCGTGTC
                     CGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGCTGATCTGCACC
                     ACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGGGCTACGGCGTGC
                     AGTGCTTCGCCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCAT
                     GCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAAGACGACGGCAACTACAAG
                     ACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGG
                     GCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAA
                     CAGCCACAACGTCTATATCACCGCCGACAAGCAGAAGAACGGCATCAAGGCCAACTTC
                     AAGATCCGCCACAACATCGAGGACGGCGGCGTGCAGCTCGCCGACCACTACCAGCAGA
                     ACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCTACCA
                     GTCCAAGCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTC
                     GTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGGGCTCTGGTGCTA
                     CCAACTTCTCACTGTTGAAACAGGCAGGGGATGTAGAGGAGAATCCAGGGCCTGGTGC
                     TAGTGGAGACTACAAAGACCATGACGGAGATTATAAAGATCATGACATCGATTACAAG
                     GATGACGATGACAAGTCCGGACTCAGATCTGGAGGCTCCGGAGGCCCAGAGCCAGCGA
                     AGTCTGCTCCCGCCCCGAAAAAGGGCTCCAAGAAGGCGGTGACTAAGGCGCAGAAGAA
                     AGGCGGCAAGAAGCGCAAGCGCAGCCGCAAGGAGAGCTATTCCATCTATGTGTATAAG
                     GTTCTGAAGCAGGTCCACCCTGACACCGGCATTTCGTCCAAGGCCATGGGCATCATGA
                     ACTCGTTTGTGAACGACATTTTCGAGCGCATCGCAGGTGAGGCTTCCCGCCTGGCGCA
                     TTACAACAAGCGCTCGACCATCACCTCCAGGGAGATCCAGACGGCCGTGCGCCTGCTG
                     CTGCCTGGGGAGTTGGCCAAGCACGCCGTGTCCGAGGGTACTAAGGCCATCACCAAGT
                     ACACCAGCGCTAAGTAATG"
                     /note="Geneious type: Editing History Replacement"
     source          <1140..>2409
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1140..2409
                     /created_by="ximena.opitzaraya"
                     /label="HisTag_GCaMP8s"
     misc_feature    2438..2683
                     /locus_tag="WPRE3"
                     /ApEinfo_label="WPRE3"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey=21
                     /label="WPRE3"
     misc_feature    2438..2597
                     /locus_tag="gamma"
                     /ApEinfo_label="gamma"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey=21
                     /label="gamma"
     misc_feature    2603..2683
                     /locus_tag="alpha"
                     /ApEinfo_label="alpha"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey=21
                     /label="alpha"
     misc_feature    2697..2900
                     /locus_tag="BGH-pA"
                     /ApEinfo_label="BGH-pA"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey=21
                     /label="BGH-pA"
     misc_feature    2929..3069
                     /locus_tag="R-ITR"
                     /ApEinfo_label="R-ITR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey=21
                     /label="R-ITR"
     rep_origin      3161..3467
                     /locus_tag="f1\Origin"
                     /ApEinfo_label="f1\Origin"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey=33
                     /label="f1\Origin"
     misc_feature    3986..4843
                     /locus_tag="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey=21
                     /label="AmpR"
     rep_origin      4994..5661
                     /locus_tag="pUC\ori"
                     /ApEinfo_label="pUC\ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey=33
                     /label="pUC\ori"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtcatgga tttctccaaa gctttgtagg gcagaactct
      181 gaatgtttat ttctctgatg aatccttaat gaaagtggcc agccatcctg gggagaacaa
      241 gccttagttc ccactgagaa ggatagcaag cagcataaga gtagctgatg tcaaaggcta
      301 ttaatgcaaa tgaaaattat aattcctcat caagatctgc tcccagacag gctaatcatt
      361 gtgccacctg tttacagaaa cacactgcta atcagtcagt aaaatctttt aagtttctgt
      421 gcttctctgt gtacgatttt tagcctccat ggatttctcc aaagctttgt agggcagaac
      481 tctgaatgtt tatttctctg atgaatcctt aatgaaagtg gccagccatc ctggggagaa
      541 caagccttag ttcccactga gaaggatagc aagcagcata agagtagctg atgtcaaagg
      601 ctattaatgc aaatgaaaat tataattcct catcaagatc tgctcccaga caggctaatc
      661 attgtgccac ctgtttacag aaacacactg ctaatcagtc agtaaaatct tttaagtttc
      721 tgtgcttctc tgtgtacgat ttttagcctc catggatttc tccaaagctt tgtagggcag
      781 aactctgaat gtttatttct ctgatgaatc cttaatgaaa gtggccagcc atcctgggga
      841 gaacaagcct tagttcccac tgagaaggat agcaagcagc ataagagtag ctgatgtcaa
      901 aggctattaa tgcaaatgaa aattataatt cctcatcaag atctgctccc agacaggcta
      961 atcattgtgc cacctgttta cagaaacaca ctgctaatca gtcagtaaaa tcttttaagt
     1021 ttctgtgctt ctctgtgtac gatttttagc ctcgagctcg ggctgggcat aaaagtcagg
     1081 gcagagccat ctattgctta catttgcttc tgggatccag atctttcgaa gctagccacc
     1141 atgcatcatc accatcatca cacgcgtcgc aagaagacct tcaaggaggt ggccaccgcc
     1201 gtgaagatca tcgccatgct gatgggcctg aagatcaacg tctatatcaa ggccgacaag
     1261 cagaagaacg gcatcaaggc gaacttccac atccgccaca acatcgagga cggcggcgtg
     1321 cagctcgcct accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     1381 gacaaccact acctgagcgt ggagtccaaa ctttcgaaag accccaacga gaagcgcgat
     1441 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     1501 tacaagggcg gtaccggagg gagcatggtg agcaagggcg aggagctgtt caccggggtg
     1561 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     1621 gagggtgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     1681 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
     1741 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     1801 tacatccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     1861 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     1921 gaggacggca acatcctggg gcacaagctg gagtacaacc tgccggacca actgactgaa
     1981 gagcagatcg cagaatacaa agaggctttc tccctatttg acaaggacgg ggatgggaca
     2041 ataacaacca aggagctggg gacggtgatg cggtctctcg gacacaaccc cacagaagca
     2101 gagctgcagg acatgatcaa tgaagtagat gccgacggtg acggcacaat cgacttccct
     2161 gagttcctga caatgatggc aagaaaaatg aaatacaggg acacggaaga agaaattaga
     2221 gaagcgttcg gtgtgtttga taaggatggc aatggctaca tcagtgcagc agagcttcgc
     2281 cacgtgatga caaaccttgg agagaagtta acagatgaag aggttgatga aatgatcagg
     2341 gaagcagaca tcgatgggga tggtcaggta aactacgaag agtttgtaca aatgatgaca
     2401 gcgaagtaag gcgcgccgcg gccgcgaatt cgatatcata atcaacctct ggattacaaa
     2461 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     2521 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     2581 ttgtataaat cctggttagt tcttgccacg gcggaactca tcgccgcctg ccttgcccgc
     2641 tgctggacag gggctcggct gttgggcact gacaattccg tggctcgaga gatcttcgac
     2701 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     2761 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     2821 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     2881 ggaagacaat agcaggcatg agatctcacg tgcggaccga gcggccgcag gaacccctag
     2941 tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa
     3001 aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct
     3061 gcctgcaggg gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     3121 gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     3181 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     3241 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     3301 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt
     3361 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     3421 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     3481 ctcgggctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     3541 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt
     3601 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
     3661 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     3721 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     3781 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     3841 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     3901 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     3961 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     4021 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     4081 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     4141 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     4201 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     4261 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     4321 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     4381 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     4441 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     4501 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     4561 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     4621 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     4681 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     4741 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     4801 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     4861 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     4921 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     4981 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     5041 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     5101 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     5161 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     5221 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     5281 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     5341 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     5401 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     5461 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     5521 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     5581 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     5641 cttttgctgg ccttttgctc acatgt
//
