LOCUS       pMSP1D1_with_Strep      5883 bp ds-DNA     circular     15-JUL-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             1..5883
                     /label="Translation 1-5883"
                     /translation="AEERLMRYFLLTHLCGISHRNGALSVQSALMPHS*ASIHSAIAT*LGHGCAPTPANTR*RALTGLSAPGIRLQTSCDRLRELHVSEVFTVITETREAAAVKLISVVVKRFTDVCLFIRVQLVEFLQKR*CLASDKAGHVKGGFFLFGH*CLRVRGISVHGGNDTDETREDAHDTGY***TCPVTGTL*G*TTGGMDAAGPEKNHSGSMPALR*YRCRCSTG*PAASCDADPEHNGAGR*LPRFQTLRNTETEDHSCCCSGRRRFAAAVASRSLAYR*FILLTSKATPPA*PGPQRQEHDHAHPWGRHAGDNGLLLAETFGGGTSDEGLSEGVQDSEYRKRQADHRRAPAKAVLAENDPERCRHLSYELHDKEDSHKCGDDSHAPRPPEGADWVEGSQGHRSRSRCLMSELTYINCVALTARFPVGKPVVPAALMNRPTRGERRFAYWAPGWFFFSPVRRATADCPSPPGPERVAASGPRWFAPAGENPV*WWLTAGYNMSCLRYRRIPLPRYPHQRAARTR*WRALRPAPSDRWQPASQWERCPHSAFAWFVENRTWHSSRLPVPLSAEFDCE*DIYASQPDADAPRQNLMGPLTARFAGDPMRPDAPRPVAYRLHGRK*YC*WVSGQRHQEITPEH*CRQLPQQWHPGHPADS**SAH*RVAREDCAPPLYRLRRRFVLPSTPPRWHPVDRREI*SPRQFATARAGPDWRWQRQSATTVCPPVVVPRGWECNSAPPSPLPLFPAFSQKRGWPGSPRGKRSDKRHRHTLRHRITLLVSHSPP*IDSLPGAIMPYRERFCAIRWCPGSRRSPLCDSCIRKQPSSRLRPLSTAAARNGACKEMAPNSPPATGPATIPTPKQALMSPKWRARSSPSVMSAI*APATAPVAPVMPATMRPA*RIEISIPRN*YDSL*GNCERITIPL*K*FCLTLRRRYTMGWSHPQFEKDYDIPTTENLYFQGSTFSKLREQLGPVTQEFWDNLEKETEGLRQEMSKDLEEVKAKVQPYLDDFQKKWQEEMELYRQKVEPLRAELQEGARQKLHELQEKLSPLGEEMRDRARAHVDALRTHLAPYSDELRQRLAARLEALKENGGARLAEYHAKATEHLSTLSEKAKPALEDLRQGLLPVLESFKVSFLSALEEYTKKLNTQ*ACGRTRAPPPPPLRSGC*QSPKGS*VGCCHR*AITSITPWGL*TGLEGFFAERRNYIRIGEWDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*GDGSRSGPSP**TVFRPLTLESTFFNSGLLFQTGTTLNPISVYSFDL*GILPISAYWLKNELI*QKFNANFNKILTLTI*VALFGEMCAEPLFVYFSKYIQICIRS*INS*KNSSSIK*NCNLFISGLSIPYF*KSRFCNEGENSPRQFHRMARSWYRSAIPTRPTSIQPINFPSSKIRLSSEKSP*VTTESGENGKSLCISFQTCSTGQPLRSSSKSLASTKPLFIRDCA*ARRNTRSLLKGQLQTGIECNRRRNTASASTIFSPESGYSSNTWNAVFPGIAVVSNHASSGVRIKCLMVGRGINSVSQFSLTISSVTSLATLPLPCFRNNSGASGFPYNR*IVAPDCPTLSRAHLYPYKSASMLEFNRGLEQDVSR*IWLITPLVLLFM*ADSFIVHDQNPLT*VFVPLSVRPRRKDQRIFLKSFFSARNLLLANKKTTATSGGLFAGSRATNSFSEGNWLQQSADTKYCPSSVAVVRPPLQELCSTAYIPRSANPVTSGCCQWR*VVSYRVGLKTIVTG*GAAVGLNGGFVHTAQLGANDLHRTEIPTA*AMRKRHASRREKGGQVSGKRQGRNRRAHEGASRGKRLVSL*SCRVSPPLT*ASIFVMLVRGAEPMEKRQQRGLFTVPGLLLAFCSHVLSCVIP*FCG*PYYRL*VS*YRSPQPNDRAQRVSERG"
     misc_feature    12..151
                     /label="bom"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: basis of mobility region from pBR322"
     CDS             complement(253..444)
                     /label="rop"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="gene: rop codon_start: 1 product: Rop protein, which maintains plasmids at low copy number translation: VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHADELYRSCLARFGDDGENL* transl_table: 1"
     CDS             complement(1253..2335)
                     /label="lacI"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="gene: lacI note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG). codon_start: 1 product: lac repressor translation: VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ* transl_table: 1"
     promoter        complement(2336..2413)
                     /label="lacI promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="gene: lacI note: "
     promoter        2722..2740
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2741..2765
                     /label="lac operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG). bound_moiety: lac repressor encoded by lacI"
     RBS             2780..2802
                     /label="RBS"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)"
     misc_feature    2809..2811
                     /label="Start Codon"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    2809..3450
                     /label="MSP1D1"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    2815..2838
                     /label="Strep-II tag"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             2860..2880
                     /label="TEV site"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="codon_start: 1 product: tobacco etch virus (TEV) protease recognition and cleavage site translation: ENLYFQG transl_table: 1"
     misc_feature    3448..3450
                     /label="Stop Codon"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             3470..3487
                     /label="6xHis"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="codon_start: 1 product: 6xHis affinity tag translation: HHHHHH transl_table: 1"
     terminator      3554..3601
                     /label="T7 terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: transcription terminator for bacteriophage T7 RNA polymerase"
     rep_origin      3638..4093
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="note: f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis direction: RIGHT"
     CDS             complement(4185..5000)
                     /label="KanR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="gene: aph(3')-Ia note: confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes codon_start: 1 product: aminoglycoside phosphotransferase translation: MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF* transl_table: 1"
     rep_origin      5122..5710
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="note: high-copy-number ColE1/pMB1/pBR322/pUC origin of replication direction: RIGHT"
ORIGIN
        1 gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
       61 aatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc
      121 gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc
      181 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
      241 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta
      301 aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag
      361 ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag
      421 ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg
      481 ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca
      541 tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca
      601 gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca
      661 gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt
      721 ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt
      781 cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg
      841 ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat
      901 gcgcacccgt ggggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt
      961 ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga
     1021 caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc
     1081 tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat
     1141 agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg
     1201 tcgagatccc ggtgcctaat gagtgagcta acttacatta attgcgttgc gctcactgcc
     1261 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     1321 gagaggcggt ttgcgtattg ggcgccaggg tggtttttct tttcaccagt gagacgggca
     1381 acagctgatt gcccttcacc gcctggccct gagagagttg cagcaagcgg tccacgctgg
     1441 tttgccccag caggcgaaaa tcctgtttga tggtggttaa cggcgggata taacatgagc
     1501 tgtcttcggt atcgtcgtat cccactaccg agatatccgc accaacgcgc agcccggact
     1561 cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc atcgcagtgg
     1621 gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa accggacatg gcactccagt
     1681 cgccttcccg ttccgctatc ggctgaattt gattgcgagt gagatattta tgccagccag
     1741 ccagacgcag acgcgccgag acagaactta atgggcccgc taacagcgcg atttgctggt
     1801 gacccaatgc gaccagatgc tccacgccca gtcgcgtacc gtcttcatgg gagaaaataa
     1861 tactgttgat gggtgtctgg tcagagacat caagaaataa cgccggaaca ttagtgcagg
     1921 cagcttccac agcaatggca tcctggtcat ccagcggata gttaatgatc agcccactga
     1981 cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc ttcgacgccg cttcgttcta
     2041 ccatcgacac caccacgctg gcacccagtt gatcggcgcg agatttaatc gccgcgacaa
     2101 tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac gccaatcagc aacgactgtt
     2161 tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc atcgccgctt
     2221 ccactttttc ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg cgggaaacgg
     2281 tctgataaga gacaccggca tactctgcga catcgtataa cgttactggt ttcacattca
     2341 ccaccctgaa ttgactctct tccgggcgct atcatgccat accgcgaaag gttttgcgcc
     2401 attcgatggt gtccgggatc tcgacgctct cccttatgcg actcctgcat taggaagcag
     2461 cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag
     2521 atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg
     2581 ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg
     2641 ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc
     2701 gagatctcga tcccgcgaaa ttaatacgac tcactatagg ggaattgtga gcggataaca
     2761 attcccctct agaaataatt ttgtttaact ttaagaagga gatataccat gggtTGGAGC
     2821 CATCCGCAGT TTGAAAAAga ttatgatatt cctactactg agaatttgta ttttcagggt
     2881 tctaccttca gtaaacttcg cgaacaactg ggccccgtga cgcaggaatt ctgggacaac
     2941 ctggaaaaag aaaccgaggg actgcgtcag gaaatgtcca aagatttaga agaggtgaag
     3001 gccaaggttc agccatatct cgatgacttt cagaaaaaat ggcaggaaga gatggaatta
     3061 tatcgtcaaa aggtggaacc gctgcgtgcg gaactgcaag agggggcacg ccaaaaactc
     3121 catgagctcc aagagaagct cagcccatta ggcgaagaaa tgcgcgatcg cgcccgtgca
     3181 catgttgatg cactccggac tcatttggcg ccgtattcgg atgaacttcg ccagcgtttg
     3241 gccgcacgtc tcgaggcgct gaaagaaaac gggggtgccc gcttggctga gtaccacgcg
     3301 aaagcgacag aacacctgag caccttgagc gaaaaagcga aaccggcgct ggaagatcta
     3361 cgccagggct tattgcctgt tcttgagagc tttaaagtca gttttctgtc agctctggaa
     3421 gaatatacta aaaagctgaa tacccagtaa gcttgcggcc gcactcgagc accaccacca
     3481 ccaccactga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac
     3541 cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt
     3601 gctgaaagga ggaactatat ccggattggc gaatgggacg cgccctgtag cggcgcatta
     3661 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     3721 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     3781 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     3841 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     3901 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     3961 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     4021 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     4081 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     4141 ttttctaaat acattcaaat atgtatccgc tcatgaatta attcttagaa aaactcatcg
     4201 agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa
     4261 agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc
     4321 tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg
     4381 tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat
     4441 ggcaaaagtt tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca
     4501 tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgagacga
     4561 aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg
     4621 aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg
     4681 aatgctgttt tcccggggat cgcagtggtg agtaaccatg catcatcagg agtacggata
     4741 aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca
     4801 tctgtaacat cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg
     4861 ggcttcccat acaatcgata gattgtcgca cctgattgcc cgacattatc gcgagcccat
     4921 ttatacccat ataaatcagc atccatgttg gaatttaatc gcggcctaga gcaagacgtt
     4981 tcccgttgaa tatggctcat aacacccctt gtattactgt ttatgtaagc agacagtttt
     5041 attgttcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     5101 agaaaagatc aaaggatctt cttgaaatcc tttttttctg cgcgtaatct gctgcttgca
     5161 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     5221 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     5281 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     5341 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     5401 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     5461 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     5521 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     5581 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     5641 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     5701 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     5761 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     5821 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     5881 gga
//