LOCUS       pJExpress_KDAC8_        5109 bp    DNA        circular     17-JUL-2024
DEFINITION  HD8_optEc [cloned in pJexpress401]
ACCESSION   DNA2.0 Id: 61231
SOURCE      Synthetic
  ORGANISM  Synthetic
COMMENT     
COMMENT     ApEinfo:methylated:0
FEATURES             Location/Qualifiers
     misc_feature    2..805
                     /product="pUC_ori"
                     /locus_tag="pUC_ori"
                     /label="pUC_ori"
                     /ApEinfo_label="pUC_ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1281..1306
                     /product="pTF3"
                     /locus_tag="pTF3"
                     /label="pTF3"
                     /ApEinfo_label="pTF3"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    complement(2865..2881)
                     /product="pTR"
                     /locus_tag="pTR"
                     /label="pTR"
                     /ApEinfo_label="pTR"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1455..1502
                     /product="T5 promoter"
                     /locus_tag="T5 promoter"
                     /label="T5 promoter"
                     /ApEinfo_label="T5 promoter"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    3141..3935
                     /product="Kanamycin"
                     /locus_tag="KanR"
                     /label="KanR"
                     /ApEinfo_label="KanR"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="#00ff00"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    4030..5100
                     /product="lacI"
                     /locus_tag="lacI"
                     /label="lacI"
                     /ApEinfo_label="lacI"
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="#0000ff"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1569..1582
                     /locus_tag="RBS"
                     /label="RBS"
                     /ApEinfo_label="RBS"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2714..2761
                     /locus_tag="Tev-His"
                     /label="Tev-His"
                     /ApEinfo_label="Tev-His"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1583..2713
                     /locus_tag="HD8"
                     /label="HD8"
                     /ApEinfo_label="HD8"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1883..1885
                     /locus_tag="D101A"
                     /label="D101A"
                     /ApEinfo_label="D101A"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1882..1882
                     /locus_tag="Silent mutation"
                     /label="Silent mutation"
                     /ApEinfo_label="Silent mutation"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 CTCATGACCA AAATCCCTTA ACGTGAGTTA CGCGCGCGTC GTTCCACTGA GCGTCAGACC
       61 CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT
      121 TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA
      181 CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTTCTTCTAG
      241 TGTAGCCGTA GTTAGCCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC
      301 TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG
      361 ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA
      421 CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT
      481 GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG
      541 TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC
      601 CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC
      661 GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC
      721 CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG
      781 CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA
      841 GCGAGGAAGC GGAAGGCGAG AGTAGGGAAC TGCCAGGCAT CAAACTAAGC AGAAGGCCCC
      901 TGACGGATGG CCTTTTTGCG TTTCTACAAA CTCTTTCTGT GTTGTAAAAC GACGGCCAGT
      961 CTTAAGCTCG GGCCCCCTGG GCGGTTCTGA TAACGAGTAA TCGTTAATCC GCAAATAACG
     1021 TAAAAACCCG CTTCGGCGGG TTTTTTTATG GGGGGAGTTT AGGGAAAGAG CATTTGTCAG
     1081 AATATTTAAG GGCGCCTGTC ACTTTGCTTG ATATATGAGA ATTATTTAAC CTTATAAATG
     1141 AGAAAAAAGC AACGCACTTT AAATAAGATA CGTTGCTTTT TCGATTGATG AACACCTATA
     1201 ATTAAACTAT TCATCTATTA TTTATGATTT TTTGTATATA CAATATTTCT AGTTTGTTAA
     1261 AGAGAATTAA GAAAATAAAT CTCGAAAATA ATAAAGGGAA AATCAGTTTT TGATATCAAA
     1321 ATTATACATG TCAACGATAA TACAAAATAT AATACAAACT ATAAGATGTT ATCAGTATTT
     1381 ATTATGCATT TAGAATAAAT TTTGTGTCGC CCTTAATTGT GAGCGGATAA CAATTACGAG
     1441 CTTCATGCAC AGTGAAATCA TGAAAAATTT ATTTGCTTTG TGAGCGGATA ACAATTATAA
     1501 TATGTGGAAT TGTGAGCGCT CACAATTCCA CAACGGTTTC CCTCTAGAAA TAATTTTGTT
     1561 TAACTTTTAG GAGGTAAAAC ATATGGAAGA GCCTGAAGAG CCTGCAGACA GCGGCCAATC
     1621 CCTGGTGCCG GTTTACATTT ACAGCCCGGA ATATGTGAGC ATGTGCGATA GCTTGGCGAA
     1681 AATCCCTAAG CGCGCGAGCA TGGTCCACAG CCTGATCGAG GCGTATGCGT TGCACAAACA
     1741 AATGCGTATT GTGAAACCGA AAGTGGCATC TATGGAGGAA ATGGCTACCT TTCACACGGA
     1801 CGCGTACCTG CAACATCTGC AGAAAGTGAG CCAAGAAGGT GACGATGATC ACCCGGACAG
     1861 CATTGAGTAT GGCCTGGGTT ATGCATGTCC GGCCACGGAG GGTATTTTCG ACTACGCGGC
     1921 AGCAATCGGT GGTGCGACGA TCACCGCGGC ACAGTGTCTG ATCGATGGCA TGTGCAAGGT
     1981 TGCCATCAAT TGGAGCGGCG GTTGGCATCA CGCGAAGAAA GACGAAGCAT CCGGTTTCTG
     2041 CTACCTGAAC GATGCGGTTC TGGGCATCCT GCGTCTGCGC CGTAAGTTTG AACGCATTCT
     2101 GTACGTTGAC CTGGATCTGC ATCACGGTGA TGGCGTCGAG GACGCCTTCT CCTTTACGAG
     2161 CAAAGTCATG ACCGTGTCTC TGCACAAGTT CAGCCCGGGT TTCTTTCCGG GTACCGGCGA
     2221 TGTTAGCGAT GTTGGTCTGG GTAAGGGCCG TTACTACAGC GTGAACGTTC CGATTCAGGA
     2281 TGGTATCCAG GACGAGAAAT ACTATCAGAT CTGCGAAAGC GTGCTGAAGG AAGTTTACCA
     2341 GGCTTTCAAT CCGAAAGCCG TTGTCTTGCA GCTGGGTGCC GACACGATCG CAGGTGACCC
     2401 GATGTGCAGC TTCAATATGA CCCCGGTGGG TATTGGTAAG TGTTTGAAAT ACATTCTGCA
     2461 ATGGCAGCTG GCGACGCTGA TTCTGGGTGG CGGTGGCTAC AACTTGGCTA ACACCGCGCG
     2521 TTGTTGGACC TATTTGACCG GTGTCATCCT GGGCAAGACC CTGAGCTCGG AAATTCCGGA
     2581 CCACGAGTTT TTCACCGCTT ATGGCCCGGA TTATGTACTG GAGATCACTC CGTCTTGCCG
     2641 TCCGGACCGC AACGAGCCAC ACCGTATTCA ACAAATTCTG AACTATATCA AAGGTAATCT
     2701 GAAGCACGTC GTCGAGAATC TGTATTTTCA GGGCCATCAT CATCACCATC ACTAACTCGA
     2761 GCCCCAAGGG CGACACCCCC TAATTAGCCC GGGCGAAAGG CCCAGTCTTT CGACTGAGCC
     2821 TTTCGTTTTA TTTGATGCCT GGCAGTTCCC TACTCTCGCA TGGGGAGTCC CCACACTACC
     2881 ATCGGCGCTA CGGCGTTTCA CTTCTGAGTT CGGCATGGGG TCAGGTGGGA CCACCGCGCT
     2941 ACTGCCGCCA GGCAAACAAG GGGTGTTATG AGCCATATTC AGGTATAAAT GGGCTCGCGA
     3001 TAATGTTCAG AATTGGTTAA TTGGTTGTAA CACTGACCCC TATTTGTTTA TTTTTCTAAA
     3061 TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT
     3121 GAAAAAGGAA GAATATGAGC CATATTCAAC GGGAAACGTC GAGGCCGCGA TTAAATTCCA
     3181 ACATGGATGC TGATTTATAT GGGTATAAAT GGGCTCGCGA TAATGTCGGG CAATCAGGTG
     3241 CGACAATCTA TCGCTTGTAT GGGAAGCCCG ATGCGCCAGA GTTGTTTCTG AAACATGGCA
     3301 AAGGTAGCGT TGCCAATGAT GTTACAGATG AGATGGTCAG ACTAAACTGG CTGACGGAAT
     3361 TTATGCCACT TCCGACCATC AAGCATTTTA TCCGTACTCC TGATGATGCA TGGTTACTCA
     3421 CCACTGCGAT CCCCGGAAAA ACAGCGTTCC AGGTATTAGA AGAATATCCT GATTCAGGTG
     3481 AAAATATTGT TGATGCGCTG GCAGTGTTCC TGCGCCGGTT GCACTCGATT CCTGTTTGTA
     3541 ATTGTCCTTT TAACAGCGAT CGCGTATTTC GCCTCGCTCA GGCGCAATCA CGAATGAATA
     3601 ACGGTTTGGT TGATGCGAGT GATTTTGATG ACGAGCGTAA TGGCTGGCCT GTTGAACAAG
     3661 TCTGGAAAGA AATGCATAAA CTTTTGCCAT TCTCACCGGA TTCAGTCGTC ACTCATGGTG
     3721 ATTTCTCACT TGATAACCTT ATTTTTGACG AGGGGAAATT AATAGGTTGT ATTGATGTTG
     3781 GACGAGTCGG AATCGCAGAC CGATACCAGG ATCTTGCCAT CCTATGGAAC TGCCTCGGTG
     3841 AGTTTTCTCC TTCATTACAG AAACGGCTTT TTCAAAAATA TGGTATTGAT AATCCTGATA
     3901 TGAATAAATT GCAGTTTCAT TTGATGCTCG ATGAGTTTTT CTAAGCGGCG CGCCATCGAA
     3961 TGGCGCAAAA CCTTTCGCGG TATGGCATGA TAGCGCCCGG AAGAGAGTCA ATTCAGGGTG
     4021 GTGAATATGA AACCAGTAAC GTTATACGAT GTCGCAGAGT ATGCCGGTGT CTCTTATCAG
     4081 ACCGTTTCCC GCGTGGTGAA CCAGGCCAGC CACGTTTCTG CGAAAACGCG GGAAAAAGTG
     4141 GAAGCGGCGA TGGCGGAGCT GAATTACATT CCCAACCGCG TGGCACAACA ACTGGCGGGC
     4201 AAACAGTCGT TGCTGATTGG CGTTGCCACC TCCAGTCTGG CCCTGCACGC GCCGTCGCAA
     4261 ATTGTCGCGG CGATTAAATC TCGCGCCGAT CAACTGGGTG CCAGCGTGGT GGTGTCGATG
     4321 GTAGAACGAA GCGGCGTCGA AGCCTGTAAA GCGGCGGTGC ACAATCTTCT CGCGCAACGC
     4381 GTCAGTGGGC TGATCATTAA CTATCCGCTG GATGACCAGG ATGCCATTGC TGTGGAAGCT
     4441 GCCTGCACTA ATGTTCCGGC GTTATTTCTT GATGTCTCTG ACCAGACACC CATCAACAGT
     4501 ATTATTTTCT CCCATGAGGA CGGTACGCGA CTGGGCGTGG AGCATCTGGT CGCATTGGGT
     4561 CACCAGCAAA TCGCGCTGTT AGCGGGCCCA TTAAGTTCTG TCTCGGCGCG TCTGCGTCTG
     4621 GCTGGCTGGC ATAAATATCT CACTCGCAAT CAAATTCAGC CGATAGCGGA ACGGGAAGGC
     4681 GACTGGAGTG CCATGTCCGG TTTTCAACAA ACCATGCAAA TGCTGAATGA GGGCATCGTT
     4741 CCCACTGCGA TGCTGGTTGC CAACGATCAG ATGGCGCTGG GCGCAATGCG CGCCATTACC
     4801 GAGTCCGGGC TGCGCGTTGG TGCGGATATC TCGGTAGTGG GATACGACGA TACCGAAGAT
     4861 AGCTCATGTT ATATCCCGCC GTTAACCACC ATCAAACAGG ATTTTCGCCT GCTGGGGCAA
     4921 ACCAGCGTGG ACCGCTTGCT GCAACTCTCT CAGGGCCAGG CGGTGAAGGG CAATCAGCTG
     4981 TTGCCAGTCT CACTGGTGAA AAGAAAAACC ACCCTGGCGC CCAATACGCA AACCGCCTCT
     5041 CCCCGCGCGT TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC
     5101 GGGCAGTGA     
//
