LOCUS       pAAV                    5566 bp    DNA     circular     18-JUL-2024
FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /note="AAV2"
     source          1..5566
                     /dnas_title="pAAV NOTCH2NLCpolyG GFP "
     misc_feature    150..155
                     /note="MLU1"
     misc_feature    156..807
                     /note="CMV"
     misc_feature    808..813
                     /note="SAC2"
     misc_feature    825..872
                     /note=""
     misc_feature    873..1251
                     /note="Intron"
     misc_feature    1252..1307
                     /note=""
     misc_feature    1323..1328
                     /note="ECOR1"
     misc_feature    1330..1335
                     /note="HIND3"
     misc_feature    1339..1506
                     /note="uORF NOTCH2NLC"
     misc_feature    1354..1392
                     /note=""
     misc_feature    1507..1512
                     /note="BAMH1"
     misc_feature    1513..2226
                     /note="GFP op"
     misc_feature    2233..2238
                     /note="ECOR1"
     misc_feature    2266..2271
                     /note="XHO1"
     misc_feature    2272..2298
                     /note="HA"
     misc_feature    2305..2309
                     /note="BGL2"
     misc_feature    2323..2789
                     /note="hGH polyA+"
     misc_feature    2807..2812
                     /note="PML1"
     misc_feature    2829..2969
                     /note="AAV2LTR"
     misc_feature    3044..3504
                     /note="F1"
     misc_feature    3750..4746
                     /note="AMP R"
     misc_feature    4901..5520
                     /note="PBR322 ORI"
ORIGIN      
        1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCAAAG CCCGGGCGTC
       61 GGGCGACCTT TGGTCGCCCG GCCTCAGTGA GCGAGCGAGC GCGCAGAGAG GGAGTGGCCA
      121 ACTCCATCAC TAGGGGTTCC TGCGGCCGCA CGCGTCTAGT TATTAATAGT AATCAATTAC
      181 GGGGTCATTA GTTCATAGCC CATATATGGA GTTCCGCGTT ACATAACTTA CGGTAAATGG
      241 CCCGCCTGGC TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAATGA CGTATGTTCC
      301 CATAGTAACG cCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC
      361 TGCCCACTTG GCAGTACATC AAGTGTATCA TATGCCAAGT ACGCCCCCTA TTGACGTCAA
      421 TGACGGTAAA TGGCCCGCCT GGCATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC
      481 TTGGCAGTAC ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA
      541 CATCAATGGG CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA
      601 CGTCAATGGG AGTTTGTTTT gGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA
      661 CTCCGCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT ATATAAGCAG
      721 AGCTCGTTTA GTGAACCGTC AGATCGCCTG GAGACGCCAT CCACGCTGTT TTGACCTCCA
      781 TAGAAGACAC CGGGACCGAT CCAGCCTCCG CGGATTCGAA TCCCGGCCGG GAACGGTGCA
      841 TTGGAACGCG GATTCCCCGT GCCAAGAGTG ACgtaagtac cgcctataga gtctataggc
      901 ccacaaaaaa tgctttcttc ttttaatata cttttttgtt tatcttattt ctaatacttt
      961 ccctaatctc tttctttcag ggcaataatg atacaatgta tcatgcctct ttgcaccatt
     1021 ctaaagaata acagtgataa tttctgggtt aaggcaatag caatatttct gcatataaat
     1081 atttctgcat ataaattgta actgatgtaa gaggtttcat attgctaata gcagctacaa
     1141 tccagctacc attctgcttt tattttatgg ttgggataag gctggattat tctgagtcca
     1201 agctaggccc ttttgctaat catgttcata cctcttatct tcctcccaca gCTCCTGGGC
     1261 AACGTGCTGG TCTGTGTGCT GGCCCATCAC TTTGGCAAAG AATTGGGatt cgaacatcga
     1321 ttGAATTCaA AGCTTgccAT GTGGATCTGC CCAGGAGGAG GAGGAGGAGG AGGCGGCGGC
     1381 GGCGGAGGCG GCGACCGAGA AGATGCCCGC CCTGCGCCGC TCTGCTGTGG GCGCTGCTGG
     1441 CGCTCTGGCT GTGCTGCGCG ACCCCCGCGC ATGCATTGCA GTGTCGAGAT GGCTATGAAC
     1501 CCTGTGGGAT CCGTCAGCAA AGGCGAGGAG CTTTTCACCG GGGTTGTTCC CATCCTCGTC
     1561 GAGCTCGACG GCGACGTAAA CGGCCACAAA TTCAGCGTTT CCGGCGAGGG CGAGGGCGAT
     1621 GCCACCTACG GCAAGCTCAC CCTCAAGTTC ATCTGCACCA CCGGCAAGCT CCCCGTCCCC
     1681 TGGCCCACCC TCGTCACCAC CCTCACCTAC GGCGTCCAGT GCTTCAGCCG CTACCCCGAC
     1741 CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC
     1801 ACCATCTTCT TCAAGGACGA CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC
     1861 GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC
     1921 CTGGGGCACA AGCTGGAGTA CAACTACAAC AGCCACAACG TCTATATCAT GGCCGACAAG
     1981 CAGAAGAACG GCATCAAGGT GAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCAGCGTG
     2041 CAGCTCGCCG ACCACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC
     2101 GACAACCACT ACCTGAGCAC CCAGTCCGCC CTGAGCAAAG ACCCCAACGA GAAGCGCGAT
     2161 CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA CTCTCGGCAT GGACGAGCTG
     2221 TACAAGtgat aaGAATTCtg cagatatcca gcacagtggc ggccgCTCGA GTACCCATAC
     2281 GATGTTCCAG ATTACGCTAA GtaaAGATCt acgggtggca tcCCTGTGAC CCCTCCCCAG
     2341 TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT
     2401 TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG
     2461 TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG
     2521 AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC
     2581 AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC
     2641 TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT
     2701 CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG
     2761 CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTGTA GGTAACCACG TGCGGACCGA
     2821 GCGGCCGCAG GAACCCCTAG TGATGGAGTT GGCCACTCCC TCTCTGCGCG CTCGCTCGCT
     2881 CACTGAGGCC GGGCGACCAA AGGTCGCCCG ACGCCCGGGC TTTGCCCGGG CGGCCTCAGT
     2941 GAGCGAGCGA GCGCGCAGCT GCCTGCAGGG GCGCCTGATG CGGTATTTTC TCCTTACGCA
     3001 TCTGTGCGGT ATTTCACACC GCATACGTCA AAGCAACCAT AGTACGCGCC CTGTAGCGGC
     3061 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC
     3121 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC
     3181 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC
     3241 GACCCCAAAA AACTTGATTT GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG
     3301 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT
     3361 GGAACAACAC TCAACCCTAT CTCGGGCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT
     3421 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTTAACAAA
     3481 ATATTAACGT TTACAATTTT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG
     3541 TTAAGCCAGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC
     3601 CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT
     3661 TCACCGTCAT CACCGAAACG CGCGAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG
     3721 GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG
     3781 CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA
     3841 CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT
     3901 TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA
     3961 GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC
     4021 GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA
     4081 ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT TGACGCCGGG
     4141 CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA
     4201 GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA
     4261 ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG
     4321 CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG
     4381 GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT AGCAATGGCA
     4441 ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA
     4501 ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT
     4561 GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA
     4621 GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG
     4681 GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT
     4741 TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT
     4801 TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA
     4861 CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA
     4921 GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG
     4981 GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC
     5041 AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG
     5101 AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC
     5161 AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG
     5221 CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC
     5281 ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA
     5341 AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT
     5401 CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG
     5461 CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG
     5521 GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGT
//

