LOCUS       pYZ025_(BB_OP-4_-_OP-21_IF1)        5594 bp    DNA     circular UNA 28-FEB-2024
DEFINITION  Generated by Gibson/Homology Cloning (BB OP-4, OP-21 IF1).
ACCESSION   urn.local.yanzhang.1s-hds0shs
VERSION     urn.local.yanzhang.1s-hds0shs
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    5577..5321
                     /Source="referenceToDocument:
                     urn:sequence:genbank:<unknown"
                     /label="BB OP-4 (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    5576^5577
                     /Original_Bases="TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAA
                     CAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCT
                     TTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTG
                     TAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTC
                     TGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTT
                     GGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCG
                     TGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTG
                     AGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAG
                     CGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTAT
                     CTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCT
                     CGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCT
                     GGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTG
                     GATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCG
                     AGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCT
                     TACGCATCTGTGCGGTATTTCACACCGCAATGGTGCACTCTCAGTACAATCTGCTCTG
                     ATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCT
                     GCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGG
                     CATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTC
                     ACCGTCATCACCGAAACGCGCGAGGCAGCTGCGGTAAAGCTCATCAGCGTGGTCGTGA
                     AGCGATTCACAGATGTCTGCCTGTTCATCCGCGTCCAGCTCGTTGAGTTTCTCCAGAA
                     GCGTTAATGTCTGGCTTCTGATAAAGCGGGCCATGTTAAGGGCGGTTTTTTCCTGTTT
                     GGTCACTGATGCCTCCGTGTAAGGGGGATTTCTGTTCATGGGGGTAATGATACCGATG
                     AAACGAGAGAGGATGCTCACGATACGGGTTACTGATGATGAACATGCCCGGTTACTGG
                     AACGTTGTGAGGGTAAACAACTGGCGGTATGGATGCGGCGGGACCAGAGAAAAATCAC
                     TCAGGGTCAATGCCAGCGCTTCGTTAATACAGATGTAGGTGTTCCACAGGGTAGCCAG
                     CAGCATCCTGCGATGCAGATCCGGAACATAATGGTGCAGGGCGCTGACTTCCGCGTTT
                     CCAGACTTTACGAAACACGGAAACCGAAGACCATTCATGTTGTTGCTCAGGTCGCAGA
                     CGTTTTGCAGCAGCAGTCGCTTCACGTTCGCTCGCGTATCGGTGATTCATTCTGCTAA
                     CCAGTAAGGCAACCCCGCCAGCCTAGCCGGGTCCTCAACGACAGGAGCACGATCATGC
                     GCACCCGTGGGGCCGCCATGCCGGCGATAATGGCCTGCTTCTCGCCGAAACGTTTGGT
                     GGCGGGACCAGTGACGAAGGCTTGAGCGAGGGCGTGCAAGATTCCGAATACCGCAAGC
                     GACAGGCCGATCATCGTCGCGCTCCAGCGAAAGCGGTCCTCGCCGAAAATGACCCAGA
                     GCGCTGCCGGCACCTGTCCTACGAGTTGCATGATAAAGAAGACAGTCATAAGTGCGGC
                     GACGATAGTCATGCCCCGCGCCCACCGGAAGGAGCTGACTGGGTTGAAGGCTCTCAAG
                     GGCATCGGTCGAGATCCCGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGC
                     GCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGG
                     CCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCAC
                     CAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGC
                     AAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACG
                     GCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCCGC
                     ACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCG
                     TTGGCAACCAGCATCGCAGTGGGAACGATGCCCTCATTCAGCATTTGCATGGTTTGTT
                     GAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGATT
                     GCGAGTGAGATATTTATGCCAGCCAGCCAGACGCAGACGCGCCGAGACAGAACTTAAT
                     GGGCCCGCTAACAGCGCGATTTGCTGGTGACCCAATGCGACCAGATGCTCCACGCCCA
                     GTCGCGTACCGTCTTCATGGGAGAAAATAATACTGTTGATGGGTGTCTGGTCAGAGAC
                     ATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGG
                     TCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCA
                     CCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGC
                     ACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGG
                     GCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTG
                     CCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGT
                     TTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACA
                     CCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATT
                     GACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGT
                     GTCCGGGATCTCGACGCTCTCCCTTATGCGACTCCTGCATTAGGAAGCAGCCCAGTAG
                     TAGGTTGAGGCCGTTGAGCACCGCCGCCGCAAGGAATGGTGCATGCAAGGAGATGGCG
                     CCCAACAGTCCCCCGGCCACGGGGCCTGCCACCATACCCACGCCGAAACAAGCGCTCA
                     TGAGCCCGAAGTGGCGAGCCCGATCTTCCCCATCGGTGATGTCGGCGATATAGGCGCC
                     AGCAACCGCACCTGTGGCGCCGGTGATGCCGGCCACGATGCGTCCGGCGTAGAGGATC
                     GAGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAA
                     CAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCGTA
                     CAGAATATTGTGGACAGCTCCGTTTGTCCCACGTGGGGCAGCAGGTGACTCTGTGTGG
                     TTGGGTCAACCGTCGTCGTGATCTTGGTAGCCTGATCTTCATCGATATGCGCGACCGC
                     GAAGGTATCGTGCAGGTATTTTTCGATCCGGATCGTGCGGACGCGTTAAAGCTGGCCT
                     CTGAACTGCGTAATGAGTTCTGCATTCAGGTCACGGGCACCGTACGTGCGCGTGACGA
                     AAAAAATATTAACCGCGATATGGCGACCGGCGAAATCGAAGTGCTGGCGTCCTCGCTG
                     ACTATCATCAACCGCGCAGATGTTCTGCCGCTTGACTCTAACCACGTCAACACCGAAG
                     AAGCGCGTCTGAAATACCGCTACCTCGACCTGCGTCGTCCGGAAATGGCTCAGCGCCT
                     GAAAACCCGCGCTAAAATCACCAGCCTGGTGCGCCGTTTTATGGATGACCACGGCTTC
                     CTCGACATCGAAACTCCGATGCTGACCAAAGCCACGCCGGAAGGCGCGCGTGACTACC
                     TGGTGCCTTCTCGTGTGCACAAAGGTAAATTCTACGCACTGCCGCAATCCCCGCAGTT
                     GTTCAAACAGCTGCTGATGATGTCCGGTTTTGACCGTTACTATCAGATCGTTAAATGC
                     TTCCGTGACGAAGACCTGCGTGCTGACCGTCAGCCTGAATTTACTCAGATCGATGTGG
                     AAACTTCTTTCATGACCGCGCCGCAAGTGCGTGAAGTGATGGAAGCGCTGGTGCGTCA
                     TCTGTGGCTGGAAGTGAAGGGTGTGGATCTGGGCGATTTCCCGGTAATGACCTTTGCG
                     GAAGCAGAACGCCGTTATGGTTCTGATAAACCGGATCTGCGTAACCCGATGGAACTGA
                     CTGACGTTGCTGATCTGCTGAAATCTGTTGAGTTTGCTGTATTTGCAGGTCCGGCGAA
                     CGATCCGAAAGGTCGCGTAGCGGCTCTGCGCGTTCCGGGCGGCGCATCGCTGACCCGT
                     AAGCAGATCGACGAATACGGTAACTTCGTTAAAATCTACGGCGCGAAAGGTCTGGCTT
                     ACATCAAAGTTAACGAACGCGCGAAAGGTCTGGAAGGTATCAACAGCCCGGTAGCGAA
                     GTTCCTTAATGCAGAAATCATCGAAGACATCCTGGATCGTACTGCCGCGCAAGATGGC
                     GATATGATTTTCTTCGGTGCCGACAACAAGAAAATTGTTGCCGACGCGATGGGTGCAC
                     TGCGCCTGAAAGTGGGTAAAGACCTTGGTCTGACCGACGAAAGCAAATGGGCACCGCT
                     GTGGGTTATCGACTTCCCGATGTTTGAAGACGACGGTGAAGGCGGCCTGACGGCAATG
                     CACCATCCGTTCACCTCACCGAAAGATATGACGGCTGCAGAACTGAAAGCTGCACCGG
                     AAAATGCGGTGGCGAACGCTTACGATATGGTCATCAATGGTTACGAAGTGGGCGGTGG
                     TTCAGTACGTATCCATAATGGTGATATGCAGCAGACGGTGTTTGGTATTCTGGGTATC
                     AACGAAGAGGAACAGCGCGAGAAATTCGGCTTCCTGCTCGACGCTCTGAAATACGGTA
                     CTCCGCCGCACGCAGGTCTGGCATTCGGTCTTGACCGTCTGACCATGCTGCTGACCGG
                     CACCGACAATATCCGTGACGTTATCGCCTTCCCGAAAACCACGGCGGCAGCGTGTCTG
                     ATGACTGAAGCACCGAGCTTTGCTAACCCGACTGCACTGGCTGAGCTGAGCATTCAGG
                     TTGTGAAGAAGGCTGAGAATAACCTCGAGCACCACCACCACCACCAC"
                     /label="TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAAC
                     CACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAA
                     GGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAG
                     TTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCC
                     TGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAG
                     ACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAG
                     CCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAG
                     AAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGT
                     CGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGT
                     CCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGG
                     GGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTG
                     CTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGT
                     ATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCG
                     AGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCT
                     GTGCGGTATTTCACACCGCAATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCAT
                     AGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGA
                     CACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTT
                     ACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATC
                     ACCGAAACGCGCGAGGCAGCTGCGGTAAAGCTCATCAGCGTGGTCGTGAAGCGATTCA
                     CAGATGTCTGCCTGTTCATCCGCGTCCAGCTCGTTGAGTTTCTCCAGAAGCGTTAATG
                     TCTGGCTTCTGATAAAGCGGGCCATGTTAAGGGCGGTTTTTTCCTGTTTGGTCACTGA
                     TGCCTCCGTGTAAGGGGGATTTCTGTTCATGGGGGTAATGATACCGATGAAACGAGAG
                     AGGATGCTCACGATACGGGTTACTGATGATGAACATGCCCGGTTACTGGAACGTTGTG
                     AGGGTAAACAACTGGCGGTATGGATGCGGCGGGACCAGAGAAAAATCACTCAGGGTCA
                     ATGCCAGCGCTTCGTTAATACAGATGTAGGTGTTCCACAGGGTAGCCAGCAGCATCCT
                     GCGATGCAGATCCGGAACATAATGGTGCAGGGCGCTGACTTCCGCGTTTCCAGACTTT
                     ACGAAACACGGAAACCGAAGACCATTCATGTTGTTGCTCAGGTCGCAGACGTTTTGCA
                     GCAGCAGTCGCTTCACGTTCGCTCGCGTATCGGTGATTCATTCTGCTAACCAGTAAGG
                     CAACCCCGCCAGCCTAGCCGGGTCCTCAACGACAGGAGCACGATCATGCGCACCCGTG
                     GGGCCGCCATGCCGGCGATAATGGCCTGCTTCTCGCCGAAACGTTTGGTGGCGGGACC
                     AGTGACGAAGGCTTGAGCGAGGGCGTGCAAGATTCCGAATACCGCAAGCGACAGGCCG
                     ATCATCGTCGCGCTCCAGCGAAAGCGGTCCTCGCCGAAAATGACCCAGAGCGCTGCCG
                     GCACCTGTCCTACGAGTTGCATGATAAAGAAGACAGTCATAAGTGCGGCGACGATAGT
                     CATGCCCCGCGCCCACCGGAAGGAGCTGACTGGGTTGAAGGCTCTCAAGGGCATCGGT
                     CGAGATCCCGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGC
                     CCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGC
                     GGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGAC
                     GGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCC
                     ACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATAT
                     AACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCCGCACCAACGCG
                     CAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACC
                     AGCATCGCAGTGGGAACGATGCCCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGG
                     ACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGATTGCGAGTGAG
                     ATATTTATGCCAGCCAGCCAGACGCAGACGCGCCGAGACAGAACTTAATGGGCCCGCT
                     AACAGCGCGATTTGCTGGTGACCCAATGCGACCAGATGCTCCACGCCCAGTCGCGTAC
                     CGTCTTCATGGGAGAAAATAATACTGTTGATGGGTGTCTGGTCAGAGACATCAAGAAA
                     TAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGC
                     GGATAGTTAATGATCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTT
                     TACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCACCCAGTTG
                     ATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTG
                     GAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGT
                     TGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGA
                     AACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATAC
                     TCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTT
                     CCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCCGGGAT
                     CTCGACGCTCTCCCTTATGCGACTCCTGCATTAGGAAGCAGCCCAGTAGTAGGTTGAG
                     GCCGTTGAGCACCGCCGCCGCAAGGAATGGTGCATGCAAGGAGATGGCGCCCAACAGT
                     CCCCCGGCCACGGGGCCTGCCACCATACCCACGCCGAAACAAGCGCTCATGAGCCCGA
                     AGTGGCGAGCCCGATCTTCCCCATCGGTGATGTCGGCGATATAGGCGCCAGCAACCGC
                     ACCTGTGGCGCCGGTGATGCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCTCG
                     ATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCC
                     TCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCGTACAGAATATT
                     GTGGACAGCTCCGTTTGTCCCACGTGGGGCAGCAGGTGACTCTGTGTGGTTGGGTCAA
                     CCGTCGTCGTGATCTTGGTAGCCTGATCTTCATCGATATGCGCGACCGCGAAGGTATC
                     GTGCAGGTATTTTTCGATCCGGATCGTGCGGACGCGTTAAAGCTGGCCTCTGAACTGC
                     GTAATGAGTTCTGCATTCAGGTCACGGGCACCGTACGTGCGCGTGACGAAAAAAATAT
                     TAACCGCGATATGGCGACCGGCGAAATCGAAGTGCTGGCGTCCTCGCTGACTATCATC
                     AACCGCGCAGATGTTCTGCCGCTTGACTCTAACCACGTCAACACCGAAGAAGCGCGTC
                     TGAAATACCGCTACCTCGACCTGCGTCGTCCGGAAATGGCTCAGCGCCTGAAAACCCG
                     CGCTAAAATCACCAGCCTGGTGCGCCGTTTTATGGATGACCACGGCTTCCTCGACATC
                     GAAACTCCGATGCTGACCAAAGCCACGCCGGAAGGCGCGCGTGACTACCTGGTGCCTT
                     CTCGTGTGCACAAAGGTAAATTCTACGCACTGCCGCAATCCCCGCAGTTGTTCAAACA
                     GCTGCTGATGATGTCCGGTTTTGACCGTTACTATCAGATCGTTAAATGCTTCCGTGAC
                     GAAGACCTGCGTGCTGACCGTCAGCCTGAATTTACTCAGATCGATGTGGAAACTTCTT
                     TCATGACCGCGCCGCAAGTGCGTGAAGTGATGGAAGCGCTGGTGCGTCATCTGTGGCT
                     GGAAGTGAAGGGTGTGGATCTGGGCGATTTCCCGGTAATGACCTTTGCGGAAGCAGAA
                     CGCCGTTATGGTTCTGATAAACCGGATCTGCGTAACCCGATGGAACTGACTGACGTTG
                     CTGATCTGCTGAAATCTGTTGAGTTTGCTGTATTTGCAGGTCCGGCGAACGATCCGAA
                     AGGTCGCGTAGCGGCTCTGCGCGTTCCGGGCGGCGCATCGCTGACCCGTAAGCAGATC
                     GACGAATACGGTAACTTCGTTAAAATCTACGGCGCGAAAGGTCTGGCTTACATCAAAG
                     TTAACGAACGCGCGAAAGGTCTGGAAGGTATCAACAGCCCGGTAGCGAAGTTCCTTAA
                     TGCAGAAATCATCGAAGACATCCTGGATCGTACTGCCGCGCAAGATGGCGATATGATT
                     TTCTTCGGTGCCGACAACAAGAAAATTGTTGCCGACGCGATGGGTGCACTGCGCCTGA
                     AAGTGGGTAAAGACCTTGGTCTGACCGACGAAAGCAAATGGGCACCGCTGTGGGTTAT
                     CGACTTCCCGATGTTTGAAGACGACGGTGAAGGCGGCCTGACGGCAATGCACCATCCG
                     TTCACCTCACCGAAAGATATGACGGCTGCAGAACTGAAAGCTGCACCGGAAAATGCGG
                     TGGCGAACGCTTACGATATGGTCATCAATGGTTACGAAGTGGGCGGTGGTTCAGTACG
                     TATCCATAATGGTGATATGCAGCAGACGGTGTTTGGTATTCTGGGTATCAACGAAGAG
                     GAACAGCGCGAGAAATTCGGCTTCCTGCTCGACGCTCTGAAATACGGTACTCCGCCGC
                     ACGCAGGTCTGGCATTCGGTCTTGACCGTCTGACCATGCTGCTGACCGGCACCGACAA
                     TATCCGTGACGTTATCGCCTTCCCGAAAACCACGGCGGCAGCGTGTCTGATGACTGAA
                     GCACCGAGCTTTGCTAACCCGACTGCACTGGCTGAGCTGAGCATTCAGGTTGTGAAGA
                     AGGCTGAGAATAACCTCGAGCACCACCACCACCACCAC"
                     /note="Geneious type: Editing History Deletion"
     CDS             5586..41
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="39.682539682539684"
                     /fragment="True"
                     /other="CDS"
                     /label="SET1"
     terminator      49..96
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
                     /label="T7 terminator"
     rep_origin      133..588
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
                     /label="f1 ori"
     promoter        614..718
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /label="AmpR promoter"
     CDS             719..1579
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /label="AmpR"
     misc_feature    1750..5321
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     rep_origin      1750..2338
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
                     /label="ori"
     misc_feature    2524..2663
                     /note="pLannotate"
                     /database="addgene"
                     /identity="98.592"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc feature"
                     /label="bom"
     CDS             complement(2765..2956)
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /label="rop"
     CDS             complement(3425..3716)
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="24.517212426532325"
                     /fragment="True"
                     /other="CDS"
                     /label="TcR"
     CDS             complement(3765..4847)
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /label="lacI"
     promoter        complement(4848..4925)
                     /note="pLannotate"
                     /database="addgene"
                     /identity="97.5"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /label="lacI promoter"
     CDS             complement(4933..5212)
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="23.509655751469353"
                     /fragment="True"
                     /other="CDS"
                     /label="TcR"
     promoter        5234..5252
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /label="T7 promoter"
     protein_bind    5253..5277
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein bind"
                     /label="lac operator"
     RBS             5292..5314
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="RBS"
                     /label="RBS"
     ligation        5300..5321
                     /label="Ligation"
     misc_feature    5300..5594
                     /label="OP-21 IF1 (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     ORF             5322..5576
                     /Genetic_code="Standard"
                     /Reading_frame=1
                     /created_by="ORF Finder"
                     /label="ORF 4 (frame 1)"
     primer_bind     5322..5341
                     /annotation_group="12549733711935afac306b-604a-4ef2-8a07-4
                     14a2aecb0ee"
                     /Sequence="ATGAGAGGATCGCATCACCA"
                     /created_by="primer3"
                     /Product_Size=255
                     /#_Local_Off-target_Sites=0
                     /Local_Off-target="OP-21 IF1"
                     /Extension="ACTTTAAGAAGGAGATATACAT"
                     /Tm_(binding_region)="58.6"
                     /Tm_(with_extension)="62.0"
                     /%GC_(binding_region)="50.0"
                     /%GC_(with_extension)="38.1"
                     /Hairpin_Tm_(with_extension)="36.9"
                     /Self_Dimer_Tm_(with_extension)="7.2"
                     /Pair_Dimer_Tm_(binding_region)="None"
                     /label="OP-21 IF1 (1 F) - BB OP-4"
     CDS             5334..5351
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /label="6xHis"
     source          <5358..>5573
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From=" IF1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; source
                     synthetic DNA construct (<a
                     href=""urn:local:zoila:9i-e1bfodt""> IF1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     source synthetic DNA construct ( IF1 translation)"
                     /label="source synthetic DNA construct"
     CDS             5358..5573
                     /created_by="zoila"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From=" IF1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; IF1 (<a
                     href=""urn:local:zoila:9i-e1bfodt""> IF1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; IF1
                     ( IF1 translation)"
                     /label="IF1"
     ORF             complement(5452..>5576)
                     /codon_start=3
                     /Genetic_code="Standard"
                     /Reading_frame=3
                     /created_by="ORF Finder"
                     /label="ORF 33 (frame 3)"
     primer_bind     complement(5557..5576)
                     /annotation_group="12550007054594868d4b6b-53f2-4880-a525-5
                     7e2cc53f082"
                     /Sequence="TCAGCGACTACGGAAGACAA"
                     /created_by="primer3"
                     /Product_Size=255
                     /#_Local_Off-target_Sites=0
                     /Local_Off-target="OP-21 IF1"
                     /Extension="GTTAGCAGCCGGATCTCA"
                     /Tm_(binding_region)="58.8"
                     /Tm_(with_extension)="67.3"
                     /%GC_(binding_region)="50.0"
                     /%GC_(with_extension)="52.6"
                     /Hairpin_Tm_(with_extension)="43.2"
                     /Self_Dimer_Tm_(with_extension)="23.9"
                     /Pair_Dimer_Tm_(binding_region)="None"
                     /label="OP-21 IF1 (255 R) - BB OP-4"
     ligation        5577..5594
                     /label="Ligation"
ORIGIN      
        1 aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc
       61 cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga
      121 ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
      181 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
      241 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
      301 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
      361 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
      421 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
      481 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
      541 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
      601 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      661 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
      721 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
      781 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
      841 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
      901 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
      961 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     1021 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     1081 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     1141 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     1201 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     1261 tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     1321 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     1381 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     1441 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     1501 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     1561 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     1621 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     1681 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     1741 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     1801 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     1861 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     1921 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     1981 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     2041 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     2101 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     2161 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     2221 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     2281 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     2341 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     2401 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     2461 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     2521 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcaatggtgc
     2581 actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc
     2641 tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac
     2701 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     2761 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat
     2821 cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga
     2881 gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt
     2941 tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga
     3001 taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt
     3061 tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa
     3121 tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc
     3181 agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt
     3241 ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg
     3301 ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag
     3361 taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc
     3421 gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac
     3481 cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga
     3541 tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca
     3601 cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc
     3661 cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc
     3721 ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc
     3781 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     3841 gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga
     3901 ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc
     3961 agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg
     4021 gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg
     4081 gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg
     4141 ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc
     4201 cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc
     4261 agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat
     4321 gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg
     4381 atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc
     4441 acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc
     4501 gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac
     4561 accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac
     4621 ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc
     4681 agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt
     4741 tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa
     4801 gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg
     4861 aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg
     4921 gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag
     4981 taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc
     5041 caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag
     5101 cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac
     5161 cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc
     5221 gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct
     5281 ctagaaataa ttttgtttaa ctttaagaag gagatataca tatgagagga tcgcatcacc
     5341 atcaccatca cggatccatg gccaaagaag acaatattga aatgcaaggt accgttcttg
     5401 aaacgttgcc taataccatg ttccgcgtag agttagaaaa cggtcacgtg gttactgcac
     5461 acatctccgg taaaatgcgc aaaaactaca tccgcatcct gacgggcgac aaagtgactg
     5521 ttgaactgac cccgtacgac ctgagcaaag gccgcattgt cttccgtagt cgctgatgag
     5581 atccggctgc taac
//
