LOCUS       Exported                5194 bp ds-DNA     circular SYN 25-NOV-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5194)
  AUTHORS   Tivan69
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 18, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5194
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(318..1196)
                     /codon_start=1
                     /label=araC
                     /note="/vntifkey=4"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1223..1507
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     promoter        1226..1498
                     /label=PBAD
                     /note="/vntifkey=30"
     primer_bind     1339..1359
                     /label=NT79
     primer_bind     complement(1520..1540)
                     /label=NT74
     misc_feature    1527..1532
                     /label=RBS
     CDS             1541..2299
                     /codon_start=1
                     /label=XpCdnC_CD-NTase
                     /translation="MGDQYLNGILAREAVDVSLLSPAFSAKATLTPVINAWGGNLVQSV
                     SPSGSFAKGTANASGTDVDLFISLHPSTTHGLREIYITLFNALRDVGYTVRKQDVSLGI
                     RVGLIDVDLVPGKQQTPLTTDHSLYRSRADTWTKTNVQTHIDVVRAAGRQSETRIIKLW
                     RDQNKLEFPSVYLEFVVIRALQLNFSGSLAERVWTVLRFLADNFAAVRVVDPANGSNVI
                     SDDLTLAEKLAIERAAKFSLTQQTWGSIVR"
     CDS             2296..3015
                     /codon_start=1
                     /label=Effector_Endonuclease_NucC
                     /translation="MTQASLAKLLDHLHVQVEAELKTARSALHHPTDKGDASEQVWINL
                     LSNYLPRRYVVSKAHVVDSEGNFSQQIDVVVHDRQYSPLVFTFQGLHYVPAESVYAIFE
                     AKQETNAQTVNYAQTKAESVRRLIRTSMPVPTVDGPKPAKEPGHILAGLLSLSCPWKPP
                     LGEALACHVHARGGDSQLDMGCIADAGIFFMNDEGALEVQPGARAATRFLLELIARLQE
                     LGTVPMLDIRSYARHIL"
     misc_feature    2512..2514
                     /product="D>N mutation"
                     /label=Active site residue
                     /note="> metal coordination> D to N mutation inactivates"
     misc_feature    2605..2607
                     /label=Active site residue
                     /note="> metal coordination"
     primer_bind     3016..3042
                     /label=NT73
     terminator      3033..3104
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      3165..3192
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     primer_bind     complement(3187..3209)
                     /label=NT94
     promoter        3211..3302
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3303..4163
                     /codon_start=1
                     /label=Ampicillin
                     /note="/vntifkey=4"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4308..4981
                     /direction=RIGHT
                     /label=pBR322 ori
                     /label=pBR322\ori
                     /note="/vntifkey=33"
ORIGIN
        1 atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga
       61 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
      121 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
      181 aaacgcgcga ggcagcagat caattcgcgc gcgaaggcga agcggcatgc ataatgtgcc
      241 tgtcaaatgg acgaagcagg gattctgcaa accctatgct actccgtcaa gccgtcaatt
      301 gtctgattcg ttaccaatta tgacaacttg acggctacat cattcacttt ttcttcacaa
      361 ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag
      421 agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc
      481 aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct
      541 aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg
      601 ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt
      661 acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac
      721 aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg
      781 ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag
      841 aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga
      901 cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga
      961 atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga
     1021 tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc
     1081 ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc
     1141 agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt
     1201 ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt
     1261 cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc
     1321 attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat
     1381 cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca
     1441 tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt
     1501 tctccatacc cgttttttgg gctaacagga ggaattaacc atgggcgacc agtaccttaa
     1561 tgggattctc gcgcgcgagg ccgttgatgt gtctttgctc tcacctgcat tctcagcgaa
     1621 ggcaacgctg acgccggtaa tcaatgcctg gggcggcaac ctagtgcagt cggttagtcc
     1681 aagcggatcg ttcgcgaagg gtacggcgaa cgccagcggc accgatgtgg atctgtttat
     1741 ctccttgcat ccctctacca cccacggcct cagggagatt tacatcacgc tcttcaatgc
     1801 tctgagagat gttggctaca cggtacgtaa gcaggacgtg tccctaggca tacgcgtggg
     1861 tctgatcgat gtggatctgg ttcccggaaa gcaacagacg ccactcacca ccgaccatag
     1921 cctctatcgc agccgggctg atacatggac caagaccaac gttcaaactc acatcgacgt
     1981 ggtccgcgca gccggacggc agtctgagac gagaatcatc aagctttggc gagatcagaa
     2041 caagttggaa ttcccatcgg tctacttgga attcgtggtg atcagggcct tgcagttgaa
     2101 cttctccggc agtttggccg agcgggtttg gacggtactg cggttcttgg ccgacaattt
     2161 tgcggctgtt cgagtcgttg atccggctaa tggaagcaat gtgatttcgg acgaccttac
     2221 gctcgcagag aagcttgcta tcgagcgcgc cgcgaaattt tcgcttacgc aacagacatg
     2281 gggcagcatc gtccgatgac acaggcatcg ctagctaaac ttctggatca cctgcacgta
     2341 caggtggagg cagagctgaa gactgcccgc tcagcgttgc atcaccccac cgacaagggc
     2401 gacgccagtg agcaggtttg gatcaactta ctgagcaact acttgcccag gcgctacgtg
     2461 gtgagcaaag cgcatgtggt cgatagcgag ggtaacttca gccagcagat cgatgtggtc
     2521 gtacacgatc gccagtattc cccattggta ttcaccttcc agggattgca ttatgtgcct
     2581 gcggaaagtg tctatgcgat ctttgaggcc aagcaagaga ccaacgcgca aacggtgaac
     2641 tatgcgcaga ccaaagcaga gagcgtgagg aggctgatca ggactagcat gccagtgccc
     2701 acggtagacg gccccaagcc ggccaaggaa ccgggccaca tcctggccgg tctgctgtca
     2761 ttgagctgcc cttggaagcc tccgctagga gaggctttgg cctgccatgt tcacgccagg
     2821 ggcggggact cgcagctcga tatgggctgt atcgcagatg cgggtatatt tttcatgaac
     2881 gatgagggtg cgctggaagt ccagcctgga gcacgcgcag caacgcgatt cctcctcgag
     2941 ctaatagcca ggttgcagga gctgggaaca gttccgatgc tggatatccg cagttacgcc
     3001 agacacatcc tgtaataaga attcccaggc atcaaataaa acgaaaggct cagtcgaaag
     3061 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctccagctg gtaccatatg
     3121 gcgggcagga cgcccgccat aaactgccag gcatcaaatt aagcagaagg ccatcctgac
     3181 ggatggcctt tttgcgtttc tacaaactct tttgtttatt tttctaaata cattcaaata
     3241 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     3301 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     3361 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     3421 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     3481 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     3541 cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     3601 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     3661 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     3721 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     3781 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     3841 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     3901 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     3961 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     4021 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     4081 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     4141 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     4201 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     4261 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     4321 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     4381 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     4441 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     4501 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     4561 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     4621 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     4681 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     4741 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     4801 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     4861 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     4921 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     4981 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     5041 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     5101 aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat
     5161 ggtgcactct cagtacaatc tgctctgatg ccgc
//
