LOCUS       p1412-_A2               6734 bp    DNA     circular SYN 20-JUL-2024
DEFINITION  p1412-_A2.
ACCESSION   p1412-_A2
VERSION     p1412-_A2
KEYWORDS    .
SOURCE      
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     rep_origin      complement(2539..2967)
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     enhancer        complement(4954..5333)
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     polyA_signal    complement(3013..3237)
                     /note="pLannotate"
                     /label="bGH poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        complement(4750..4953)
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(1338..2129)
                     /note="pLannotate"
                     /label="neo"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     polyA_signal    complement(1040..1161)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        5599..5703
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             5704..6564
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      1..589
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        complement(2196..2525)
                     /note="pLannotate"
                     /label="SV40 promoter"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        913..943
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(3429..4646)
                     /note="pLannotate"
                     /label="SLC17A3"
                     /database="swissprot"
                     /identity="40.3"
                     /match_length="96.7"
                     /fragment="False"
                     /other="CDS"
     protein_bind    877..898
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        complement(4687..4705)
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        3263..3281
                     /note="pLannotate"
                     /label="SP6 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    951..967
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             complement(1156..1314)
                     /note="pLannotate"
                     /label="ble (fragment)"
                     /database="swissprot"
                     /identity="98.1"
                     /match_length="42.1"
                     /fragment="True"
                     /other="CDS"
     misc_feature    5488..5540
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="37.6"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(3320..3344)
                     /note="pLannotate"
                     /label="3xFLAG (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="34.7"
                     /fragment="True"
                     /other="CDS"
     CDS             772..864
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
     LTR             complement(4721..4754)
                     /note="pLannotate"
                     /label="5' LTR (truncated) (fragment)"
                     /database="snapgene"
                     /identity="97.1"
                     /match_length="18.8"
                     /fragment="True"
                     /other="LTR"
     promoter        complement(2988..3009)
                     /note="pLannotate"
                     /label="SV40 promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="6.1"
                     /fragment="True"
                     /other="promoter"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttt gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgacggtata cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     1081 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     1141 ataagctgca ataaacaagt tggggtgggc gaagaactcc agcatgagat ccccgcgctg
     1201 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
     1261 ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa
     1321 ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa
     1381 tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct
     1441 tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg
     1501 ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca
     1561 tcgccatggg tcacgacgag atcctcgccg tcgggcatgc gcgccttgag cctggcgaac
     1621 agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg
     1681 gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag
     1741 gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg
     1801 gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag
     1861 tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc
     1921 agccacgata gccgcgctgc ctcgtcctgc agttcattca gggcaccgga caggtcggtc
     1981 ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag
     2041 ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa
     2101 cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatca
     2161 gatccgaaaa tggatataca agctcccggg agctttttgc aaaagcctag gcctccaaaa
     2221 aagcctcctc actacttctg gaatagctca gaggcagagg cggcctcggc ctctgcataa
     2281 ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc
     2341 gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc
     2401 atacttctgc ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga
     2461 tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga
     2521 cacacattcc acagaattaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     2581 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     2641 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     2701 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     2761 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     2821 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     2881 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     2941 cgcttaatgc gccgctacag ggcgcgtggg gataccccct agagccccag ctggttcttt
     3001 ccgcctcaga agccatagag cccaccgcat ccccagcatg cctgctattg tcttcccaat
     3061 cctccccctt gctgtcctgc cccaccccac cccccagaat agaatgacac ctactcagac
     3121 aatgcgatgc aatttcctca ttttattagg aaaggacagt gggagtggca ccttccaggg
     3181 tcaaggaagg cacgggggag gggcaaacaa cagatggctg gcaactagaa ggcacagtcg
     3241 aggctgatca gcgagctcta gcatttaggt gacactatag aatagggccc tctagatgca
     3301 tgctcgagcc atcattaaac cttatcgtcg tcatccttgt aatcactgcc agatccgcca
     3361 gtttgtaact ttatgtcctc agaggcgggt aagggtcctc tctttggccc agtcttgaag
     3421 ttctgcttgt ccaaacgtga ggtaaaagac caggccaaac atgttgactg cagcagacag
     3481 gaaaaagaca ttcctccaac cagactcaaa atcctgactg atgaggaatc cagtggcagt
     3541 ggaagagatg attcctgcga tgagcccaaa tccccttgag attcccatga ggaaacttgc
     3601 atatcaagag atgaaaagag ctttcgcaca gtgatcaatc tgagaagatt cctggacaaa
     3661 aggaaatctg ccagctgacc tcctaaaatt gtacagcttg cagcagcaat aaaaggcagg
     3721 gaggacagaa ctccactatc tctgatgtta acatggagca gagtactgat atacgttggt
     3781 aggtatgtta ggatgatggt gcacaaccag aaatggctga aaaaacccag gaaaatggcc
     3841 caaagtggta ggcatgtgac catcgccttt atggggacag ctcgtccagg agaactgggc
     3901 tgttgagcca gtgaggacag gatgtgctcc ttttccctaa cacttatgca cgggtgatgc
     3961 atggggtcat cataaatcac tgtgaaccat aggagacagc agacacagcc agtgctacca
     4021 aagatgtaga agataaaagg ccagctcaag gcctgtgaga ttagtccccc cacacagagg
     4081 atgatgaagg atccaaatgc tgaccctgat cctgcaatgg tggtgagctt gcttcgttca
     4141 agtggaggag cccactttgc ccaaatagta aactgacctg tccatgccat tccctgggcc
     4201 atgccctgga ctgtccgaac catgatgacc aaaatcactc cgaagtcagc agccagtggt
     4261 gtaaagaggg tgagaaggga agagatcagc aaaccagcac caagcatttt ttttgctcca
     4321 aatatccctg ctaaatatcc acttgggatc agagtcagta ttatcccata gttgatggag
     4381 ctaaagatga taccctgagt ttctgggctc cattgataca cagaggcctt tgtatcaaat
     4441 tccttgatgg atatgctgga gttattgaag gcatctgcaa caggcccctc agtggaggca
     4501 ttagatagac cttgctgctg agtggtgttc accatggcga tgatcgcaat gctcagactc
     4561 acacgctgcg ttatcatggt gaagtttgag aagtgcatga taagagccag cccatagcgt
     4621 aatgaacaga aatctggacc tttcctggtg gcaggcttcc cgtccatcta gataagcttg
     4681 ggtctcccta tagtgagtcg tattaatttc gataagccag taagcagtgg gttctctagt
     4741 tagccagaga gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt
     4801 caatggggcg gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa
     4861 actcccattg acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc
     4921 acgcccattg atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta
     4981 gatgtactgc caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg
     5041 ccatttaccg tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac
     5101 tgccaagtgg gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat
     5161 tggcgttact atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg
     5221 tcagccaggc gggccattta ccgtaagtta tgtaacgcgg aactccatat atgggctatg
     5281 aactaatgac cccgtaattg attactatta ataactagtc aataatcaat gtcaacgcgt
     5341 atatctggcc cgtacatcgc gaagcagcgc aaaacgccta accctaagca gattcttcat
     5401 gcaattgtcg gtcaagcctt gccttgttgt agcttaaatt ttgctcgcgc actactcagc
     5461 gacctccaac acacaagcag ggagcagata ctggcttaac tatgcggcat cagagcagat
     5521 tgtactgaga gtgcaccata ggggatcggg agatctcccg atccgtcgac gtcaggtggc
     5581 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     5641 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     5701 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     5761 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     5821 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     5881 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     5941 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     6001 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     6061 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     6121 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     6181 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     6241 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     6301 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     6361 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     6421 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     6481 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     6541 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     6601 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     6661 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     6721 atcaaaggat cttc
//
