LOCUS       Pet28a-AzaD             8567 bp ds-DNA     circular     18-JUL-2024
DEFINITION  .
ACCESSION   urn.local...hl-hz7s0hd
KEYWORDS    .
SOURCE      .
  ORGANISM  .
COMMENT     ApEinfo:methylated:1.
FEATURES             Location/Qualifiers
     promoter        9..27
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="0-pET-28a"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; T7
                     promoter (<a
                     href=""urn:local:.:br-hz7ptc3"">0-pET-28a</a>)"
                     /label="T7 promoter"
     misc_binding    28..50
                     /locus_tag="LacO"
                     /ApEinfo_label="LacO"
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="LacO"
     protein_bind    28..52
                     /bound_moiety="<i>lac</i> repressor encoded by
                     <i>lacI</i>"
                     /note="The <i>lac</i> repressor binds to the <i>lac</i>
                     operator to inhibit transcription in <i>E. coli</i>. This
                     inhibition can be relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="0-pET-28a"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lac
                     operator (<a
                     href=""urn:local:.:br-hz7ptc3"">0-pET-28a</a>)"
                     /label="lac operator"
     RBS             83..88
                     /note="ribosome binding site"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="0-pET-28a"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; RBS (<a
                     href=""urn:local:.:br-hz7ptc3"">0-pET-28a</a>)"
                     /label="RBS"
     CDS             96..98
                     /product="start codon"
                     /transl_table=1
                     /Transferred_Translation="M"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="0-pET-28a"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ATG (<a
                     href=""urn:local:.:br-hz7ptc3"">0-pET-28a</a>)"
                     /label="ATG"
     CDS             108..125
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /transl_table=1
                     /Transferred_Translation="HHHHHH"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="0-pET-28a"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; 6xHis
                     (<a href=""urn:local:.:br-hz7ptc3"">0-pET-28a</a>)"
                     /label="6xHis"
     CDS             135..152
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /transl_table=1
                     /Transferred_Translation="LVPRGS"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="0-pET-28a"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; thrombin
                     site (<a href=""urn:local:.:br-hz7ptc3"">0-pET-28a</a>)"
                     /label="thrombin site"
     gene            159..3413
                     /locus_tag="AzaD"
                     /ApEinfo_label="AzaD"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /modified_by="Devon"
                     /label="azaD"
     CDS             3435..3452
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /transl_table=1
                     /Transferred_Translation="HHHHHH"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="0-pET-28a"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; 6xHis
                     (<a href=""urn:local:.:br-hz7ptc3"">0-pET-28a</a>)"
                     /label="6xHis"
     terminator      3519..3566
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="0-pET-28a"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; T7
                     terminator (<a
                     href=""urn:local:.:br-hz7ptc3"">0-pET-28a</a>)"
                     /label="T7 terminator"
     rep_origin      3603..4058
                     /snapgene_direction="LEFT"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="0-pET-28a"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; f1 ori
                     (<a href=""urn:local:.:br-hz7ptc3"">0-pET-28a</a>)"
                     /label="f1 ori"
     CDS             complement(4151..4966)
                     /codon_start=1
                     /gene="<i>aph(3')-Ia</i>"
                     /note="confers resistance to kanamycin"
                     /product="aminoglycoside phosphotransferase"
                     /transl_table=1
                     /Transferred_Translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDN
                     VGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRT
                     PDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFR
                     LAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIF
                     DEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFH
                     LMLDEFF*"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="0-pET-28a"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; KanR (<a
                     href=""urn:local:.:br-hz7ptc3"">0-pET-28a</a>)"
                     /label="KanR"
     rep_origin      5088..5676
                     /snapgene_direction="LEFT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="0-pET-28a"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ori (<a
                     href=""urn:local:.:br-hz7ptc3"">0-pET-28a</a>)"
                     /label="ori"
     CDS             complement(6106..6297)
                     /codon_start=1
                     /gene="<i>rop</i>"
                     /product="Rop protein"
                     /transl_table=1
                     /Transferred_Translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDAD
                     EQADICESLHDHADELYRSCLARFGDDGENL*"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="0-pET-28a"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; rop (<a
                     href=""urn:local:.:br-hz7ptc3"">0-pET-28a</a>)"
                     /label="rop"
     CDS             complement(7106..8188)
                     /codon_start=1
                     /gene="<i>lacI</i>"
                     /note="The <i>lac</i> repressor binds to the <i>lac</i>
                     operator to inhibit transcription in <i>E. coli. </i>This
                     inhibition can be relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /product="<i>lac</i> repressor"
                     /transl_table=1
                     /Transferred_Translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSA
                     KTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQL
                     GASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFL
                     DVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLT
                     RNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRV
                     GADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVS
                     LVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="0-pET-28a"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacI (<a
                     href=""urn:local:.:br-hz7ptc3"">0-pET-28a</a>)"
                     /label="lacI"
     promoter        complement(8189..8266)
                     /gene="<i>lacI</i>"
                     /note="<br>"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="0-pET-28a"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacI
                     promoter (<a
                     href=""urn:local:.:br-hz7ptc3"">0-pET-28a</a>)"
                     /label="lacI promoter"
ORIGIN      
        1 cgcgaaatta atacgactca ctatagggga attgtgagcg gataacaatt cccctctaga
       61 aataattttg tttaacttta agaaggagat ataccatggg cagcagccat catcatcatc
      121 atcacagcag cggcctggtg ccgcgcggca gccatatgac cgacactccc gccgccgtcc
      181 tcgccgacct ccgcgaagcg ggcctgtcgc tgtccgccgc gggcggcgac ctcaggctcg
      241 aaggccccaa gacggccatg acccccgacc tcgtcgcgag gctcaaggcc gccaaggccg
      301 acctcgtcgc gcacctggcc gcgttcgagg acgccttcgc gctgacgccc ctgcaacgcg
      361 cctacctggt cggacggtcc ggcctgctcg aactcggcga cgtcgccaac caggtctacc
      421 acgagatcga aggcgcctgg gacctcgacc gactgcgaaa gtcccttgag gacaccgcac
      481 gggcccactc ggcgctgcga ctggccgtcc tcgacgagga ccggcaggcc gagaccccca
      541 ccctgcccgg cttcaccgta acggacctgc gcggcctcga cccgagcgag cgggaccggc
      601 gccgcgccgc cgtccgcgag cgccgctccc accgcaggct cccggccacc gggcccctcg
      661 tcgatgccga ggtctgcgtc ctcgccgacg accggatggt cctgtgcgtc aaccacgacg
      721 gcatggccgt cgacggcatc ggcatgttcc tcctcttcgg cgaatggcac cgccgatacg
      781 agaccggcgg cggcgacgac gcgccgcgga tgcccttccg cgcccacgtg gcggcgatcg
      841 aggccgcggc cgccggcccc gcctccgccc gctcgcgcga gtactggcgc tcgcgcctgg
      901 acgaactccc tcccgcgccc cggctgccgc tcgccgccga cccgtccgcg atcggcccca
      961 cccgggtcgt ccagcgccgg gtggtgctcg aggccgacga ctggacccgc ctccagcgcg
     1021 agatcaaggc cgccgggctc accccctcgg ccgcgctgct cgccgcctgg gccgaggcac
     1081 tgtccctgtg gggcgccgga aacgcgttca cgctcaacac caccgtctcg cagcgcctcc
     1141 cgatccaccc cggcgccttc accgccgtcg gccagttctc cgacccgctc ctcgtcgccg
     1201 tccacatcga tcggacccgg accttcgccg aacgcgcccg cgccctccag ggccgactcc
     1261 gcaccgacct cgaccaccgc cactactccg gcatcgacgt catgcgcgac ctcgcacgcc
     1321 accactcgga cacccgcgcc gccgccatgc cctacacctt caactccaca ctggacgcca
     1381 tcggcggcgt cgacggctcc gcgctcgaat cgttcggccc cgaggtcttc accgtctccc
     1441 agaccccgca ggtccacctc gacgtgttcg tcctccagca gcacggcgac ctcgtcatcc
     1501 gcctcgacgc ggtcgaagcg ctcttcccgc ccggcctgct cgacgccctc accgccggac
     1561 tcgacaccct cctgcaccgc ctctgcgacg caaccgcctg ggacgccgaa cggttcgacc
     1621 tcctgccgga cgcgcagcgc cgcgcccgca agcgcgccaa cgacaccgag cgggacttcg
     1681 cgcccgccat gctcaccgac gccttcctgc ggcacgccgc gcagcgcccc gacgcgaccg
     1741 cgatcatcac cgcggacgcg tcgatcacct acggcgacct cctcgcccgc gccgcgggcg
     1801 ccgcgacctg gctgcgcgac aacggcgccg gccgcggcga cctcgtcggc ctggtgatgc
     1861 ggcgcggacc cgagcagatc gtcggcatcc tcggcgccct gctcgccggc gccgcgtacg
     1921 tcccggtcga cgccgacctc ccctcgaccc gccgcgaccg gctcctgcgc gacgaccgcc
     1981 tcgcccacgt cctcaccgac gacgacctgg atcacggcct cccgtcgctc gacctgcgcg
     2041 cggggccgcg cgcggccgcg gcccccgacg tgcccggcgc cgaccccggc gacctcgcct
     2101 acgtcctcta cacctcgggc accaccggcg aacccaaggg cgtcatggtg acccaccgga
     2161 acgtcgccaa cgtcgtcgcc gactgcgccg agcggttcgc gatcggcccg gacgaccgct
     2221 tcttcgccat cagcgccttc accttcgacc tgtcggtgtg ggacgtcttc ggcgccctga
     2281 gcgccggggc cgcgctggtc gtcccggacg ccgaccgggc cgccgacccc gcgcactgga
     2341 agacgctcgc cgaagccgcc ggagcgacgg tgtggaactc ggttccggcg atcgtgcgga
     2401 tgctcgccga ccacgacggg tcactgccgc cctcgctgcg actggtcatg atgagcggcg
     2461 accgcatccc gcccgacctg cccgccgcgc tctaccgcgc ccgacccgac ctgcgcgtcg
     2521 tctccctcgg cgggccgacc gaaacgacga tctggaacat ctggcatccg atcccgcccg
     2581 accacccggc ggacgtgccg ctcccgtacg gccgccccaa cgccaacaac cgcgcgcaca
     2641 tccgcgacgc cgacggcaac gactgccccg actgggtccc cggcgagatc ctcgccgccg
     2701 gcgccggcgt cacccccggc taccggggcg cccccgacct gaccgccgaa cgctacgtcg
     2761 acgaccgcgg cgaccggctc taccgcaccg gcgacctcgg ccgctaccgg cccgacggca
     2821 cgatcgacat cctgggccgc accgacttcc agatcaaggt caacggccac cgcatcgagg
     2881 ccggcgaggt cgagacccgg ctcgcggccc tcgacccggt cgccgaggcc gcggtcgtgg
     2941 cccgcaaggg cgcctccggg gcggtcctcg tcgcccacct cgtccccgcc gccggccggc
     3001 ccccgctcgc cgagatccgc gaggccctcc gcgccgacct gcccgactac atggtcccgg
     3061 ccgcggtcgt gtggcacgac cggctgccgc tcaaccgcaa ccgcaaggtc gaccgggccg
     3121 cgctcgccga cgtcccgatc gccgaacagg gagccgaaga ccgcccgccc gccgaccccg
     3181 ccgtcgaatc caggatcgcc gcgctgtggg cgagcacgct gcgggtcccc gaggtcgacc
     3241 ccgaagccga cttcaccgcc ctcggcggcg actccctgac cgccgcgcgc atcctgaccg
     3301 ccgtgcgcgc cgagtacggg atcggcctgc cgctggaggc gatcttcgcg ctcaacaccg
     3361 tgcgcgccat ggcgggccac ctcaccgcca ccaccgaacg ggagcagccg tagaagcttg
     3421 cggccgcact cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa
     3481 aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct
     3541 ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga ttggcgaatg
     3601 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     3661 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     3721 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     3781 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     3841 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     3901 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     3961 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     4021 taacgcgaat tttaacaaaa tattaacgtt tacaatttca ggtggcactt ttcggggaaa
     4081 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     4141 gaattaattc ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag
     4201 gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga
     4261 ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact cgtccaacat
     4321 caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat
     4381 gagtgacgac tgaatccggt gagaatggca aaagtttatg catttctttc cagacttgtt
     4441 caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca
     4501 ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct gttaaaagga caattacaaa
     4561 caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata ttttcacctg
     4621 aatcaggata ttcttctaat acctggaatg ctgttttccc ggggatcgca gtggtgagta
     4681 accatgcatc atcaggagta cggataaaat gcttgatggt cggaagaggc ataaattccg
     4741 tcagccagtt tagtctgacc atctcatctg taacatcatt ggcaacgcta cctttgccat
     4801 gtttcagaaa caactctggc gcatcgggct tcccatacaa tcgatagatt gtcgcacctg
     4861 attgcccgac attatcgcga gcccatttat acccatataa atcagcatcc atgttggaat
     4921 ttaatcgcgg cctagagcaa gacgtttccc gttgaatatg gctcataaca ccccttgtat
     4981 tactgtttat gtaagcagac agttttattg ttcatgacca aaatccctta acgtgagttt
     5041 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     5101 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     5161 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     5221 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     5281 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     5341 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     5401 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     5461 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     5521 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     5581 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     5641 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     5701 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     5761 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     5821 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc
     5881 cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct
     5941 ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc
     6001 tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     6061 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     6121 gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga
     6181 ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa
     6241 tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga
     6301 tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag
     6361 gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg
     6421 taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca
     6481 gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca
     6541 gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg
     6601 gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct
     6661 tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc
     6721 tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggggccg ccatgccggc
     6781 gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc
     6841 gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg
     6901 aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat
     6961 gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga
     7021 gctgactggg ttgaaggctc tcaagggcat cggtcgagat cccggtgcct aatgagtgag
     7081 ctaacttaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     7141 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca
     7201 gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc accgcctggc
     7261 cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga aaatcctgtt
     7321 tgatggtggt taacggcggg atataacatg agctgtcttc ggtatcgtcg tatcccacta
     7381 ccgagatatc cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg
     7441 ccatctgatc gttggcaacc agcatcgcag tgggaacgat gccctcattc agcatttgca
     7501 tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct atcggctgaa
     7561 tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc gagacagaac
     7621 ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc
     7681 ccagtcgcgt accgtcttca tgggagaaaa taatactgtt gatgggtgtc tggtcagaga
     7741 catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg gcatcctggt
     7801 catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg
     7861 ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg ctggcaccca
     7921 gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc agggccagac
     7981 tggaggtggc aacgccaatc agcaacgact gtttgcccgc cagttgttgt gccacgcggt
     8041 tgggaatgta attcagctcc gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa
     8101 cgtggctggc ctggttcacc acgcgggaaa cggtctgata agagacaccg gcatactctg
     8161 cgacatcgta taacgttact ggtttcacat tcaccaccct gaattgactc tcttccgggc
     8221 gctatcatgc cataccgcga aaggttttgc gccattcgat ggtgtccggg atctcgacgc
     8281 tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc
     8341 accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg
     8401 gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga
     8461 tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg
     8521 atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatccc
//
