LOCUS       Exported                5145 bp ds-DNA     circular SYN 25-SEP-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5145)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jul 22, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5145
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        10..996
                     /locus_tag="sCMV IE94"
                     /label=sCMV IE94
     promoter        1022..1038
                     /locus_tag="SP6"
                     /label=SP6
     misc_feature    1078..1454
                     /locus_tag="H2B"
                     /label=H2B
     misc_feature    1462..2151
                     /locus_tag="mScarlet3"
                     /label=mScarlet3
     polyA_signal    2181..2378
                     /locus_tag="SV40 late polyA (from pCS2+)"
                     /label=SV40 late polyA (from pCS2+)
     promoter        complement(2427..2446)
                     /locus_tag="T3"
                     /label=T3
     primer_bind     complement(2462..2483)
                     /locus_tag="M13R"
                     /label=M13R
     rep_origin      complement(2810..3483)
                     /direction=LEFT
                     /locus_tag="pUC ori"
                     /label=pUC ori
     rep_origin      complement(2851..3533)
                     /direction=LEFT
                     /locus_tag="colE1 ori"
                     /label=colE1 ori
     CDS             complement(3631..4488)
                     /locus_tag="ampR"
                     /label=ampR
     rep_origin      complement(4751..5057)
                     /direction=LEFT
                     /locus_tag="f1(+) ori"
                     /label=f1(+) ori
ORIGIN
        1 tcgaccatag ccaattcaat atggcgtata tggactcatg ccaattcaat atggtggatc
       61 tggacctgtg ccaattcaat atggcgtata tggactcgtg ccaattcaat atggtggatc
      121 tggaccccag ccaattcaat atggcggact tggcaccatg ccaattcaat atggcggact
      181 tggcactgtg ccaactgggg aggggtctac ttggcacggt gccaagtttg aggaggggtc
      241 ttggccctgt gccaagtccg ccatattgaa ttggcatggt gccaataatg gcggccatat
      301 tggctatatg ccaggatcaa tatataggca atatccaata tggccctatg ccaatatggc
      361 tattggccag gttcaatact atgtattggc cctatgccat atagtattcc atatatgggt
      421 tttcctattg acgtagatag cccctcccaa tgggcggtcc catataccat atatggggct
      481 tcctaatacc gcccatagcc actcccccat tgacgtcaat ggtctctata tatggtcttt
      541 cctattgacg tcatatgggc ggtcctattg acgtatatgg cgcctccccc attgacgtca
      601 attacggtaa atggcccgcc tggctcaatg cccattgacg tcaataggac cacccaccat
      661 tgacgtcaat gggatggctc attgcccatt catatccgtt ctcacgcccc ctattgacgt
      721 caatgacggt aaatggccca cttggcagta catcaatatc tattaatagt aacttggcaa
      781 gtacattact attggaagta cgccagggta cattggcagt actcccattg acgtcaatgg
      841 cggtaaatgg cccgcgatgg ctgccaagta catccccatt gacgtcaatg gggaggggca
      901 atgacgcaaa tgggcgttcc attgacgtaa atgggcggta ggcgtgccta atgggaggtc
      961 tatataagca atgctcgttt agggaaccgc cattctgcct ggggacgtcg gagcaagctt
     1021 gatttaggtg acactataga atacaagcta cttgttcttt ttgcaggatc catggccatg
     1081 ccagagccag cgaagtctgc tcccgccccg aaaaagggct ccaagaaggc ggtgactaag
     1141 gcgcagaaga aaggcggcaa gaagcgcaag cgcagccgca aggagagcta ttccatctat
     1201 gtgtacaagg ttctgaagca ggtccaccct gacaccggca tttcgtccaa ggccatgggc
     1261 atcatgaatt cgtttgtgaa cgacattttc gagcgcatcg caggtgaggc ttcccgcctg
     1321 gcgcattaca acaagcgctc gaccatcacc tccagggaga tccagacggc cgtgcgcctg
     1381 ctgctgcctg gggagttggc caagcacgcc gtgtccgagg gtactaaggc catcaccaag
     1441 tacaccagcg ctaaggatct catggatagc accgaggcag tgatcaagga gttcatgcgg
     1501 ttcaaggtgc acatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc
     1561 gagggccgcc cctacgaggg cacccagacc gccaagctga gggtgaccaa gggtggcccc
     1621 ctgcccttct cctgggacat cctgtcccct cagttcatgt acggctccag ggccttcacg
     1681 aagcaccccg ccgacatccc cgactactgg aagcagtcct tccccgaggg cttcaagtgg
     1741 gagcgcgtga tgaacttcga ggacggcggc gccgtgtccg tggcccagga cacctccctg
     1801 gaggacggca ccctgatcta caaggtgaag ctccgcggca ccaacttccc tcctgacggc
     1861 cccgtaatgc agaagaagac aatgggctgg gaagcatcca ccgagcggtt gtaccccgag
     1921 gacgtcgtgc tgaagggcga cattaagatg gccctgcgcc tgaaggacgg cggccgctac
     1981 ctggcggact tcaagaccac ctacagggcc aagaagcccg tgcagatgcc cggcgccttc
     2041 aacatcgacc gcaagttgga catcacatcc cacaacgagg actacaccgt ggtggaacag
     2101 tacgaacgct ccgtggcccg ccactccacc ggcggctccg gtggctccta atctagaact
     2161 atagtgagtc gtattacgta gatccagaca tgataagata cattgatgag tttggacaaa
     2221 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
     2281 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
     2341 tgtttcaggt tcagggggag gtgtgggagg ttttttaatt cgcggccgcg gcgccaatgc
     2401 attgggcccg gtacccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta
     2461 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     2521 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     2581 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     2641 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     2701 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     2761 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     2821 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     2881 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     2941 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     3001 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     3061 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     3121 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     3181 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     3241 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     3301 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     3361 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     3421 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     3481 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     3541 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     3601 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     3661 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     3721 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     3781 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     3841 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     3901 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     3961 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     4021 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     4081 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     4141 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     4201 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     4261 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     4321 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     4381 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     4441 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     4501 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     4561 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctaa
     4621 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
     4681 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
     4741 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
     4801 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
     4861 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     4921 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
     4981 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
     5041 cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac
     5101 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccag
//
