LOCUS       Exported                4709 bp ds-DNA     circular SYN 10-DEC-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jul 22, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4709
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      268..295
                     /label=T2
     terminator      387..473
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      427..470
                     /label=T1
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     537..553
                     /label=M13F
     primer_bind     537..552
                     /label=M13F
     protein_bind    593..688
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_recomb     593..688
                     /label=attL4
     misc_feature    668..688
                     /label=att4_shared
     misc_feature    689..779
                     /label=pBS MCS T7-T3
                     /label=pBS MCS T7-T3(1)
     promoter        690..708
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     734..750
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        895..1697
                     /label=CMV IE enhancer/chicken B-actin promoter
     enhancer        897..1276
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     961..978
                     /label=oJDW 746
     primer_bind     complement(964..986)
                     /label=1089
     primer_bind     complement(964..986)
                     /label=oJDW 1089
     primer_bind     1045..1066
                     /label=oJDW 1198
     primer_bind     1063..1082
                     /label=oJDW 855
     primer_bind     1260..1283
                     /label=oJDW 1375 (B-actin FWD SEQ)
     gene            1278..2523
                     /label=match to X00182 chicken B-actin
     promoter        1278..1555
                     /label=chicken beta-actin promoter
     primer_bind     1310..1329
                     /label=Chicken B-actin FWD
     intron          1556..2572
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     intron          1649..2572
                     /label=chicken b-actin intron + rabbit b-globin intron
     primer_bind     1704..1723
                     /label=oJDW 747
     intron          1722..2557
                     /label=intron
     primer_bind     complement(2211..2228)
                     /label=Chicken B-actin REV
     primer_bind     complement(2238..2261)
                     /label=oJDW 1374 (B-actin REV SEQ)
     misc_feature    2496..2518
                     /label=pCAGGS FWDSeq
     primer_bind     2496..2518
                     /label=oJDW 972
     primer_bind     2526..2546
                     /label=oJDW 1202
     primer_bind     2568..2587
                     /label=BZS20
                     /note="/vntifkey=28"
     intron          2573..2625
                     /label=rabbit B-globin intron
     primer_bind     complement(2584..2606)
                     /label=oJDW 1201
     misc_feature    2664..2754
                     /label=pBS MCS T7-T3
     primer_bind     complement(2683..2699)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(2735..2754)
                     /label=T3
     promoter        complement(2736..2754)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    2754..2878
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_recomb     2755..2911
                     /label=attR1
     misc_feature    2759..2772
                     /label=att1_shared
     promoter        complement(2947..2966)
                     /label=T7
     promoter        complement(2951..2969)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2970..2990)
                     /label=M13R
     primer_bind     complement(2974..2990)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2974..2990)
                     /label=M13 R
     CDS             3103..3912
                     /label=kanR
     rep_origin      4033..4706
                     /label=pUC ori
     misc_feature    4043..4052
                     /label=TCF/LEF DBD
     rep_origin      4059..4647
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccgc gttaacgcta ccatggagct ccaaataatg
      601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt
      661 ataatgccaa ctttgtatag aaaagttggt aatacgactc actatagggc gaattgggta
      721 ccgggccccc cctcgaggtc gacggtatcg ataagcttga tatcgaattc ctgcagccca
      781 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
      841 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctggtcgaca
      901 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      961 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     1021 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     1081 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     1141 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     1201 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     1261 catcgctatt accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc
     1321 cccctcccca cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg
     1381 ggcggggggg gggggggggc gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg
     1441 ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa agtttccttt
     1501 tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc gggcgggagt
     1561 cgctgcgcgc tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg
     1621 gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg
     1681 ctgtaattag cgcttggttt aatgacggct tgtttctttt ctgtggctgc gtgaaagcct
     1741 tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc tcggggggtg cgtgcgtgtg
     1801 tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc ggcggctgtg agcgctgcgg
     1861 gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg ccgggggcgg
     1921 tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt
     1981 gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg caaccccccc tgcacccccc
     2041 tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg ggcgtggcgc
     2101 ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc
     2161 gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg ccggcggctg
     2221 tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg
     2281 acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc
     2341 tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct
     2401 tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct gtccgcgggg
     2461 ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc gtgtgaccgg
     2521 cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac agctcctggg
     2581 caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgctcg aggtcgatcg
     2641 acggtatcga taagcttgat atcgaattcc tgcagcccgg gggatccact agttctagag
     2701 cggccgccac cgcggtggag ctccagcttt tgttcccttt agtgagggtt aattcaagtt
     2761 tgtacaaaaa agttgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga
     2821 ttttgcataa aaaacagact acataatact gtaaaacaca acatatgcag tcactatgaa
     2881 ccaactactt agatggtatt agtgacctgt agaattcgag ctctagagct gcagggcggc
     2941 cgcgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg
     3001 cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca
     3061 ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg
     3121 gaaacgtcga ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg
     3181 gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg gaagcccgat
     3241 gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag
     3301 atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc
     3361 cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggaaaaac agcattccag
     3421 gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg
     3481 cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt
     3541 ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac
     3601 gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc
     3661 tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag
     3721 gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat
     3781 cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt
     3841 caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat
     3901 gagtttttct aatcagaatt ggttaattgg ttgtaacact ggcagagcat tacgctgact
     3961 tgacgggacg gcgcaagctc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac
     4021 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     4081 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     4141 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     4201 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     4261 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     4321 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     4381 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     4441 cagcgtgagc attgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     4501 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     4561 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     4621 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     4681 gccttttgct ggccttttgc tcacatgtt
//
