LOCUS       Exported                5164 bp ds-DNA     circular SYN 29-DEC-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 5164)
  AUTHORS   Wythe Lab
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jul 22, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5164
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     terminator      268..295
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      387..473
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    593..688
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     668..719
                     /label=FWD
     primer_bind     689..719
                     /label=oJDW XXX (p3E mmCdh5 FWD)
     promoter        702..3187
                     /label=mouse VE-cadherin promoter Y10887.2
     primer_bind     complement(775..796)
                     /label=mVEcP74r
                     /note="mouse VEcadherin promoter reverse primer from base 
                     74"
     primer_bind     1340..1360
                     /label=oJDW 658
     primer_bind     complement(1581..1604)
                     /label=oJDW 667
     primer_bind     2027..2048
                     /label=oJDW 659
     primer_bind     complement(2538..2558)
                     /label=oJDW 666
     primer_bind     2754..2776
                     /label=oJDW 660
     primer_bind     3004..3023
                     /label=oJDW 173
     primer_bind     3054..3077
                     /label=mVEcP2376f
                     /note="mouse VEcadherin promoter forward primer from base 
                     2376"
     primer_bind     complement(3179..3230)
                     /label=oJDW XXX (p3E mmCdh5 REV)
     primer_bind     complement(3179..3227)
                     /label=REV
     exon            3188..3202
                     /gene="Cdh5"
                     /product="VE-cadherin"
                     /number=1
     protein_bind    3210..3333
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(3406..3424)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3429..3445)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3558..4367
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      4514..5102
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccgc gttaacgcta ccatggagct ccaaataatg
      601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt
      661 ataatgccaa ctttgtatag aaaagttgct cgaggtcgac tctagtagca gaaacaaggt
      721 cctctggaag agcaactgat gctcttaggt actgaagcat catcctgccc cagagaccac
      781 tcgcatatga agcacacata ttcagtctgc cttacttgtg ttaatgattg ccagtgtccc
      841 tctgacctcc tagccctgaa aagtgtggcc tgaaggtcat ttcagagacg gggagagctg
      901 ctcagagaag ccaatcggcg agtctaggac acacagacag gatctagtcc cagagttcgc
      961 tagcctaggt gagcgtcccc tggcccctta taccacttcc ttctccagct tgcatctaat
     1021 ctgctctggc agaccatcgt gtttcctgtc ttcctggcag cctccagcac gctcagtgct
     1081 actccctgcg catgcgccct cctcccagta ccttctctga ctccagtggg cttggagtgc
     1141 gaggaggaag ggtgaggaag gggtgaaatc aggtattgga tccacagggg gtctgaagag
     1201 cactagcctg gccttttggg actgaacttc tgctatgaag acctccactg ccatccctgg
     1261 agtccggggc acatccaagg cttgctgtcc atcgtttact gtttacagat gacaacaatg
     1321 actgtgttcg gggcagaaat atccaccagg gctagagtac aaaaggagtt tgcattgatg
     1381 gccggacagg ccctgtccct ggcagcctgc cagcgctgag tatgagaccc agcgggaagt
     1441 gctaccctgg cagacgtgtc cactgagtac acagaccacc aaggcaggca gctctcgggg
     1501 aagctgtcta tgctgggcca gcccaccttg agggcaggga acagaacaga ttgtggcaga
     1561 gaggaaaatg tggagcttct gtttgttcac agacacacgc actcgcccac gcacgcacgc
     1621 acgcacgcac gcacgcacga atgcacgcac gcagtagttg aatgctatgg attccgctca
     1681 gagctgagaa cagccccagc gacagttccc tggcctctct ccttactctg atgtcctcat
     1741 ctgtcttcac atggtctcag gacgctaata ctccatccta atgtacactc ctttccctgg
     1801 gcctccgttc cagttcagtt ctcagaggac ctggagggag tgattggcta caccaacttt
     1861 gctttcgttc accaagccca tgtctctact tgggtgtcta atgggcatct ccaacattac
     1921 ctaccccaaa cagaaaaccc tttcttcccc ccaaccacac cccaccctac ccccacagta
     1981 ttttctccat gcccggaaag atctgctctc ttatggtccc tctttgcctc actgaaaagc
     2041 aggacaagtt ggggacttcc caaactttta tgcatgaaga aacccaggca atttgccaaa
     2101 aggtacactc tgggggtctg tcatttactc tgagccagaa ccctgaaatt tttactaacc
     2161 catcacataa tgaatgaaga gaatcttttt cttttttttt ttttttcttt ttttttggtt
     2221 tttcgagaca gggtttctct gtatagccct ggctatcctg gaacacactc tgtagaccag
     2281 gctggcctcg aactcagaaa tccacctgcc tctgcctccc gagtgctggg attaaaggcg
     2341 tgcgccacca cgcctggctg aatgaagaga atcttgacct catctcccca gcctcttggt
     2401 cctgagggac cctggtctac ctactgcttt gctgtcttct tagctcttct tacttttttg
     2461 ctgactcaga cctatggcta tctccattat acagatgagg agactgaggc atggatccct
     2521 ggttggtcca tggtcacgtg aagcccatca cccagtattt gtaaagtgag atgggccagg
     2581 ctggtacctt ggaactgaaa ctcacactgc cctacctgga agaatctgac aggcaaaatc
     2641 tgctgctgaa agtgattgtc tgtcacgttt ctcagctgcc cgactctgag aactccacag
     2701 ccccctttcg ttccaccata ctacagagtc gccacggaaa gccggctctg tggagaagct
     2761 gaggtagctg ggtttctgtc tgggttactc tgtccagcga ggaaacaagt accttagacc
     2821 cactaagcct ctgctttctg aactgtaaag tgggggatat gacacctgcc tcccagggat
     2881 ggctgaatgc tctggcagaa gcttagagcc cccacagcta cccctaggct cacagctcct
     2941 ccgatgagac ctagaattga ggtatgagtt gaatacccca ggcaggtcca aggcttccac
     3001 gggcccaggc tgaccaagct gaggccgccc accgtagggc ttgcctatct gcaggcagct
     3061 cacaaaggaa caataacagg aaaccatccc gaggggaagt gggccagggc cagttggaaa
     3121 acctgcctcc ctcccagcct gggtgtggct cccctctccc ctcctgaggc aatcaactgt
     3181 gctctccaca aagctcggcc ctgaattctc aagtttgtac aaaaaagttg aacgagaaac
     3241 gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac agactacata
     3301 atactgtaaa acacaacata tgcagtcact atgaatcaac tacttagatg gtattagtga
     3361 cctgtagaat tcgagctcta gagctgcagg gcggccgcga tatcccctat agtgagtcgt
     3421 attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt
     3481 acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca
     3541 gtaatacaag gggtgttatg agccatattc aacgggaaac gtcgaggccg cgattaaatt
     3601 ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag
     3661 gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg
     3721 gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg
     3781 aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac
     3841 tcaccactgc gatccccgga aaaacagcat tccaggtatt agaagaatat cctgattcag
     3901 gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt
     3961 gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga
     4021 ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac
     4081 aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc gtcactcatg
     4141 gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg
     4201 ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg
     4261 gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg
     4321 atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta
     4381 attggttgta acactggcag agcattacgc tgacttgacg ggacggcgca agctcatgac
     4441 caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga
     4501 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     4561 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     4621 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     4681 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     4741 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     4801 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     4861 tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga gaaagcgcca
     4921 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     4981 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     5041 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     5101 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     5161 tgtt
//
